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Gene information
General information
TC005083 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1880050..1881670
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005083
    iBeetle screen iB_03900

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles sometimes size shortened - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005083 RA
      MENLWQGSS LSSIEEIFD PIEIKKVVD SLVMSRFSA 
      QIMKSRTRR SRSSCSESQ DNVSLSSGR SDSSINTYN 
      LSALISNPY PQPYYYENI FTCSETDPT CELSDYCSI 
      GPSFNLMDT KLFILSQNK DQILKAIHY DDLYNKTRL 
      SKERLEAER LLLINDLTS NLLLTRDNR FEEKNELCT 
      GSVKIRNCY TWCDNQQLE EFFLCVVIC GCKLLASEL 
      TQIKSNGVI HFKEEFVVT GLRPDFTIN IKIYCASRK 
      AMKKSKQSF ACNYAPRPE EEIPLRKSL FTLWGETYT 
      TLQDLPKSN SMEVWMNSG RFTASIFSW TDLNVNLSG 
      FLNVNQSEE CPLWNREWC CLDGRYLQY FNYPPDESF 
      ECKPIGTID LATCVEFKL LSREECARK QTLFLKTYF 
      GKTVLLSCD TRTEFECWR NCIERILSA IKIWEH
    CDS sequence
    • >TC005083 RA
      atggaaaac ctctggcaa ggaagttcc cttagttca 
      attgaagaa attttcgac ccaatagaa atcaaaaaa 
      gttgtggac tctttagtc atgtcgcga tttagtgcc 
      caaattatg aaatctcgc acacgaaga tccagatct 
      tcatgttct gaaagtcaa gacaatgtc tctctttca 
      agtgggcgt tccgattcc agtataaat acttataat 
      ttatcggcg ttaatttca aatccatac ccacagcca 
      tattattat gagaatatc ttcacttgc tctgaaact 
      gaccccact tgtgaatta agcgattat tgctcaatt 
      ggtccaagc tttaattta atggacaca aaattgttc 
      attttgagt caaaataag gaccagatt ttgaaagcg 
      atacattat gacgattta tataacaaa acgagacta 
      agcaaggag cggcttgag gcggaaaga cttctttta 
      attaacgat ctgacaagt aatttatta ctaacacga 
      gacaataga tttgaagaa aaaaatgag ctgtgtaca 
      ggatcagta aaaattaga aactgctac acatggtgt 
      gataaccaa caacttgag gaatttttc ctttgtgta 
      gtaatctgt ggttgcaaa ctacttgct tccgaattg 
      actcaaata aaatcaaac ggagtgata cattttaag 
      gaagaattt gttgtaacc ggactgcga ccagatttt 
      acaatcaac attaaaatt tattgcgct agtagaaag 
      gccatgaaa aaatcgaaa caatcattt gcctgcaat 
      tacgctcca cgtccagaa gaagaaatt cctttacga 
      aaatcactt tttacctta tggggagag acctacaca 
      acattgcaa gatctccca aaatcgaat tcgatggaa 
      gtttggatg aattctgga cgttttact gccagcatt 
      ttcagttgg actgattta aatgtaaat ttgtctggt 
      tttttaaat gttaatcag agtgaagag tgtccgcta 
      tggaatagg gaatggtgt tgtttggat gggagatat 
      ttgcaatat tttaattat cctccagat gaatccttc 
      gaatgtaaa ccgataggg acaattgat ttagcaacg 
      tgtgttgaa ttcaagttg ctttcgaga gaggagtgt 
      gcgcgaaag caaacactt ttcttaaaa acatatttt 
      ggaaaaact gttttatta tcatgtgat accagaact 
      gaatttgaa tgttggcga aattgtatc gaaaggata 
      ttaagcgca atcaaaatt tgggaacac tga
    mRNA sequence
    • >TC005083 RA
      agcagtgac ttttctgca gtacatcgt gccagtaat 
      tgtgccttt tctctgaca tgaaaacaa tttacaatt 
      ctacgttta acgaaaact gtatgcccg aaaaaatgg 
      aaaacctct ggcaaggaa gttccctta gttcaattg 
      aagaaattt tcgacccaa tagaaatca aaaaagttg 
      tggactctt tagtcatgt cgcgattta gtgcccaaa 
      ttatgaaat ctcgcacac gaagatcca gatcttcat 
      gttctgaaa gtcaagaca atgtctctc tttcaagtg 
      ggcgttccg attccagta taaatactt ataatttat 
      cggcgttaa tttcaaatc catacccac agccatatt 
      attatgaga atatcttca cttgctctg aaactgacc 
      ccacttgtg aattaagcg attattgct caattggtc 
      caagcttta atttaatgg acacaaaat tgttcattt 
      tgagtcaaa ataaggacc agattttga aagcgatac 
      attatgacg atttatata acaaaacga gactaagca 
      aggagcggc ttgaggcgg aaagacttc ttttaatta 
      acgatctga caagtaatt tattactaa cacgagaca 
      atagatttg aagaaaaaa atgagctgt gtacaggat 
      cagtaaaaa ttagaaact gctacacat ggtgtgata 
      accaacaac ttgaggaat ttttccttt gtgtagtaa 
      tctgtggtt gcaaactac ttgcttccg aattgactc 
      aaataaaat caaacggag tgatacatt ttaaggaag 
      aatttgttg taaccggac tgcgaccag attttacaa 
      tcaacatta aaatttatt gcgctagta gaaaggcca 
      tgaaaaaat cgaaacaat catttgcct gcaattacg 
      ctccacgtc cagaagaag aaattcctt tacgaaaat 
      cacttttta ccttatggg gagagacct acacaacat 
      tgcaagatc tcccaaaat cgaattcga tggaagttt 
      ggatgaatt ctggacgtt ttactgcca gcattttca 
      gttggactg atttaaatg taaatttgt ctggttttt 
      taaatgtta atcagagtg aagagtgtc cgctatgga 
      atagggaat ggtgttgtt tggatggga gatatttgc 
      aatatttta attatcctc cagatgaat ccttcgaat 
      gtaaaccga tagggacaa ttgatttag caacgtgtg 
      ttgaattca agttgcttt cgagagagg agtgtgcgc 
      gaaagcaaa cacttttct taaaaacat attttggaa 
      aaactgttt tattatcat gtgatacca gaactgaat 
      ttgaatgtt ggcgaaatt gtatcgaaa ggatattaa 
      gcgcaatca aaatttggg aacactgaa cttattgat 
      tgtttgtat atgcatgtt ttgttttgt ttgtatatt 
      tcgtaataa atttatatt gaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY