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Gene information
General information
TC031598
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005078
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451761.1: 14..2105
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031598
      iBeetle screen iB_03899

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031598 RA
        MNLFSKLKE DGYFAGTFL DKSLIQFCF 
        MDIIRDELD EICLEWNSH SISSSRNSI 
        NPTGRPFVM YNMPELYES SDYLLQVPD 
        DEIEVVKNE CTFLDKGMP CDKDIFELC 
        NVLIEEGHL EKPSDPYNA VNLYVYLRT 
        QILNLL
      CDS sequence
      • >TC031598 RA
        atgaattta tttagtaaa ctaaaggaa gacggatat 
        tttgcagga acatttctt gacaagtct ctaattcag 
        ttttgtttc atggatata attcgagat gaactggac 
        gaaatatgc ttagaatgg aactcacat tcaatctca 
        agttctagg aacagtata aatccaaca ggtagaccc 
        tttgtgatg tataatatg ccagaactg tatgaatct 
        tctgattac ctattacaa gtgccagat gatgaaata 
        gaagttgtg aaaaatgag tgtacattt ttggacaaa 
        ggaatgcct tgtgataaa gatattttt gaactgtgt 
        aatgtacta atagaagaa gggcactta gaaaaacct 
        tctgatcca tacaatgca gttaatctg tacgtatat 
        ctccgaact caaatatta aacttatta taa
      mRNA sequence
      • >TC031598 RA
        tcggaattg ccagttaat tcctatgat aagcttaaa 
        ccatatggt attgcaata aatggttgc atagatgga 
        ttttctaga tccataatg tggcttgag gctggaagt 
        acaagcaat gatcctcga gttattgct ggctacttt 
        ttaaaaaca gtgtatcgt tgtggagga tgcccacgt 
        actgttcgt actgatatg ggtacaaaa aattattat 
        ctagaacaa attcaactt ttctttaag cgtcttgaa 
        gatgatgaa catgctcca ccagctttc atatatgga 
        gctagtaca gcaaatcaa aaaattgaa tgttggtgg 
        tctatccta cgcagacat aatgctcaa ttttggatg 
        aatttattt agtaaacta aaggaagac ggatatttt 
        gcaggaaca tttcttgac aagtctcta attcagttt 
        tgtttcatg gatataatt cgagatgaa ctggacgaa 
        atatgctta gaatggaac tcacattca atctcaagt 
        tctaggaac agtataaat ccaacaggt agacccttt 
        gtgatgtat aatatgcca gaactgtat gaatcttct 
        gattaccta ttacaagtg ccagatgat gaaatagaa 
        gttgtgaaa aatgagtgt acatttttg gacaaagga 
        atgccttgt gataaagat atttttgaa ctgtgtaat 
        gtactaata gaagaaggg cacttagaa aaaccttct 
        gatccatac aatgcagtt aatctgtac gtatatctc 
        cgaactcaa atattaaac ttattataa tgtaaatta 
        ttaataaac tatgttaaa gtaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY