Status
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Gene information
General information
TC005073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7147770..7188445
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005073
    iBeetle screen iB_03898

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages slightly shape rounded - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs coxa potentially shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: in all cases only one or two abdominal segments are missing.
    abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005073 RA
      MKWLGSGEG DAIVKAEMD RGPQRTQDD AAVGYVFQR 
      QPDPEFPSF GPKQLRWAL GDDSIIDNH DKWKYPVNS 
      KVGTPPNSM SYIGASSTM PGLYDLGQN KSIPSEHLV 
      YMSNQIPGG MHMHPQYLQ QPLTPQMQM RQGPIAPAA 
      KKLWGLEAK DAKSLALNH LNHEQVWRD STWAAPEHA 
      VSASLAMGR RGGFVGGDT GSILSPRGS DNGGLGVKM 
      VEYVLGGSP TNKDNIGLE PRMRALHLV DDKDKDKPQ 
      SPKEEVNGQ AVQNGQVSE DEKGFNRTP GSRQPSPAE 
      EELTKNGIS LDPTVVVMK PQEQLPPHL QHHHLPPHL 
      APHLGVTLT ESVNQQFDH FEQPPPAPY DQQPHQYPP 
      PPPQQHQVD SAVLQQQQH NFDVQAPAA LLDPLDYRQ 
      TPTCLKMLP KRYINVHQW LGPVCPKDL KAQLQLFRS 
      QQTQAATPS GAAQLQLLQ QQQQYLAQQ QQQAAFPQP 
      PYVLNHQQE PSYLITAGV PQYYGVGPW VYPAPANLI 
      QQGASNGAR RPLTPNGTA EAQQQVQPQ VPGGYIVPY 
      YDQNTPILM AQGAAMRNG TPMRLVSPA PVLVQPQAN 
      RQTPAGASL YSSTPQSLY GANVNTSVN GGTLGGVAQ 
      GVGSGLFPP LHAAPPSAP FGSSSLGNI GSSATTTSL 
      HTGLGLNTT SSGRRDSFD RNTSAFSPS LDYRQKWPT 
      SYNIGSSPS PLTGSLTPP PSAPLQLGG LMTSRVLSA 
      APGAEAKYR NSVAAGLTT NAMFGSTNS LFTKINSSL 
      STVPSSLDK GQQGRSRLL EDFRNNRYP NLQLRDLAN 
      HIVEFSQDQ HGSRFIQQK LERASATEK QMVFNEILS 
      AAYNLMTDV FGNYVIQKF FEFGTAEQK TTLAQKVRG 
      HVLPLALQM YGCRVIQKA LESIPPEQQ QEIVRELDG 
      HVLKCVKDQ NGNHVVQKC IECVDPNAL QFIIQSFSG 
      QVYTLSTHP YGCRVIQRI LEHCTPEQT APILAELHQ 
      HTDQLIQDQ FGNYVIQHV LEHGKPEDK SQLISSVRG 
      KVLALSQHK FASNVVEKC VTHATRAER ALLIEEVCG 
      FNDNALHVM MKDQYANYV VQKMIDVSE PTQRKVLMH 
      KIRPHLNSL RKYTYGKHI IAKLEKYFM KTPGSMGSI 
      GGELGPIGP PTNGVL
    CDS sequence
    • >TC005073 RA
      atgaagtgg ttgggaagc ggtgaagga gatgcaatt 
      gtaaaagct gaaatggac agagggcct cagcgcact 
      caagatgat gctgcagtt ggttatgtt ttccaaaga 
      caaccagac ccagaattt ccatctttc ggaccaaag 
      cagctgcgt tgggctctt ggagacgac agtattatc 
      gataatcat gacaagtgg aaataccca gtcaatagc 
      aaagttgga actcctccc aacagtatg tcatacatt 
      ggagcaagt tcaacgatg cccggcctt tatgattta 
      gggcaaaat aagtcaatt ccttctgaa cacttggtc 
      tatatgtcc aatcagatt ccaggaggg atgcacatg 
      catccgcag tatttgcaa caacctctt acgccacaa 
      atgcaaatg cggcaagga ccgattgct ccggcagca 
      aaaaaacta tggggtctc gaagcaaaa gacgcaaaa 
      tctttagca ttgaatcat ttaaatcac gagcaagtt 
      tggagggat tcaacatgg gcagcacca gaacatgcg 
      gtgtcggcg tctcttgcc atgggacga cgaggaggc 
      ttcgtagga ggcgatact ggcagcata ctttcgcct 
      cgagggtcc gacaacgga ggtctcggc gtcaaaatg 
      gtggaatat gtgttaggt ggttcacca actaacaaa 
      gacaacatt ggactcgaa cctaggatg cgtgccttg 
      catttggtt gatgataaa gataaagat aagccacaa 
      agtccgaaa gaggaagtc aacggacag gcggttcaa 
      aatggtcaa gtttcagaa gacgaaaag ggtttcaac 
      cgaacgccc ggtagccga caaccatcg ccggcagag 
      gaagaactt accaaaaat ggaatctcg ttggaccct 
      accgtggtg gttatgaag ccgcaagaa cagttacca 
      ccacattta caacatcat cacttacca ccccatttg 
      gcaccacat ttgggcgtg acattaacc gaatcggtt 
      aatcaacaa tttgaccat tttgaacaa ccaccgcct 
      gctccctat gaccaacaa cctcatcag tatccacca 
      cctccgccg caacaacat caagtcgac agtgccgtt 
      ctacagcaa caacaacac aattttgac gtgcaggcg 
      cccgcagcc ctgttagat cctctggat tacaggcaa 
      acgccgacc tgtctgaag atgcttccc aagagatat 
      attaacgtg caccagtgg ctaggaccg gtgtgcccc 
      aaggaccta aaagctcag cttcaactc ttccgatct 
      caacaaacc caagcggca actcctagc ggagccgcc 
      caattgcag ttgctgcaa cagcaacaa caatatctc 
      gcccaacag caacaacaa gcggcgttt cctcaacca 
      ccttacgtt cttaaccat cagcaagaa ccctcctac 
      ctcattacc gccggtgtt ccacaatat tatggtgtt 
      ggtccatgg gtatatccg gcaccggcg aatctcatc 
      caacaagga gctagcaac ggagccaga agacctctc 
      actccaaat ggcacagcc gaagcacaa cagcaagta 
      caaccgcaa gttcctggg ggttacatt gttccatac 
      tatgatcaa aatacccca attcttatg gcacaaggt 
      gcggcaatg cgaaatggt acacccatg cgcttggtt 
      agccctgct cctgtactt gtgcaacct caagctaac 
      cgacaaaca ccagcaggt gcttccctg tattctagt 
      actccccag agtttgtat ggtgctaat gtgaatact 
      tcggttaat ggaggaact ttaggaggt gtggcccaa 
      ggtgttggt tctgggtta ttcccacct cttcatgca 
      gcaccacct tctgctcca ttcggtagt tcttctcta 
      ggaaatatc ggttcatcc gctacaacc acatccctt 
      catacaggc ctgggactg aacacgaca tcgtctgga 
      cgtcgcgac tcgttcgac cggaacacg tcagcgttc 
      tcgccgtcg ctggactac agacagaaa tggccaaca 
      tcctacaac atcggcagc agtcccagt cctctaacg 
      ggaagcctg acacctccg ccgtcggcg cctctccaa 
      ctcggcgga ctgatgaca tcgcgggtt ctttcggcg 
      gcgcccgga gccgaagcg aaataccga aattccgtg 
      gcggcagga ctgacaacg aatgcgatg tttggatcg 
      acgaattcc ctcttcacg aaaataaat tcatcgtta 
      tcaacggtc cctagttcg ctggataag ggacaacag 
      ggccgatca cgcctgctc gaagacttt agaaataac 
      agatatccg aatttacaa ttgagagat ttggccaat 
      cacatcgtg gagttctcg caggaccag cacgggtcg 
      cggtttatc caacagaag ttagaacgg gcttcggcg 
      accgaaaaa cagatggtt tttaatgaa attctatcg 
      gcagcttat aatcttatg actgacgtc ttcggcaat 
      tatgttata caaaagttc ttcgaattc gggaccgct 
      gaacagaag acgacgttg gcgcagaaa gtcagaggt 
      cacgtgctt ccattagct ctacaaatg tatggttgt 
      cgggtcatt caaaaggct ctcgaatcg attccgcct 
      gaacaacag caagaaatt gtgagagaa ttggacggg 
      catgttttg aagtgcgtc aaggaccaa aacggaaat 
      catgtggtc caaaagtgc atcgaatgt gtcgacccc 
      aacgccttg cagtttatt attcagtca ttctcgggg 
      caagtgtac acgttatcg acccatccg tacggctgc 
      cgggtcatt caacgcatt ttagagcat tgcacacct 
      gagcagact gctccaatt ttagccgaa ttgcaccaa 
      cacaccgat cagttgatc caggatcag tttggaaac 
      tatgtcatc cagcatgtg ttggaacac ggaaagccc 
      gaagataag agtcagctg attagcagc gtgagaggg 
      aaagtttta gctttaagt cagcataaa ttcgccagt 
      aacgtcgtc gagaagtgc gtcactcac gcgacccgg 
      gccgagagg gcgctacta atcgaggaa gtgtgtgga 
      tttaacgac aatgctctc catgtcatg atgaaagat 
      cagtatgcg aattacgtg gttcaaaaa atgatagac 
      gtgagtgag ccaacacag cgaaaggtg ttgatgcat 
      aaaatcagg cctcatctt aactcgttg aggaaatac 
      acgtacggc aagcatatt attgccaag ttggagaaa 
      tattttatg aagactccg ggatctatg ggctctatc 
      ggaggagag ctcggtccg attggaccg ccgaccaat 
      ggagtgctc taa
    mRNA sequence
    • >TC005073 RA
      cgacgaata aaatggcga gcgatttat tcagaattt 
      gctgtcatt tttaaatct gcaaaaatc aagctgtat 
      aaatggcgt aatccgcga atcttgtac attttgtaa 
      attgtaaat agtttcttg tatagttag tggtaatat 
      tttgtgttg tgaatccga ttatagtgt ataaatctc 
      aatcgaaaa tagtgtttg tgtctccta agaaaatct 
      ttaacaaag ccgacgccg actcgtaga acattaaaa 
      atttcagaa aatacactt tgttgaagc acacggcat 
      tcatcaatt aacaacagt gacaccaat gaagtggtt 
      gggaagcgg tgaaggaga tgcaattgt aaaagctga 
      aatggacag agggcctca gcgcactca agatgatgc 
      tgcagttgg ttatgtttt ccaaagaca accagaccc 
      agaatttcc atctttcgg accaaagca gctgcgttg 
      ggctcttgg agacgacag tattatcga taatcatga 
      caagtggaa atacccagt caatagcaa agttggaac 
      tcctcccaa cagtatgtc atacattgg agcaagttc 
      aacgatgcc cggccttta tgatttagg gcaaaataa 
      gtcaattcc ttctgaaca cttggtcta tatgtccaa 
      tcagattcc aggagggat gcacatgca tccgcagta 
      tttgcaaca acctcttac gccacaaat gcaaatgcg 
      gcaaggacc gattgctcc ggcagcaaa aaaactatg 
      gggtctcga agcaaaaga cgcaaaatc tttagcatt 
      gaatcattt aaatcacga gcaagtttg gagggattc 
      aacatgggc agcaccaga acatgcggt gtcggcgtc 
      tcttgccat gggacgacg aggaggctt cgtaggagg 
      cgatactgg cagcatact ttcgcctcg agggtccga 
      caacggagg tctcggcgt caaaatggt ggaatatgt 
      gttaggtgg ttcaccaac taacaaaga caacattgg 
      actcgaacc taggatgcg tgccttgca tttggttga 
      tgataaaga taaagataa gccacaaag tccgaaaga 
      ggaagtcaa cggacaggc ggttcaaaa tggtcaagt 
      ttcagaaga cgaaaaggg tttcaaccg aacgcccgg 
      tagccgaca accatcgcc ggcagagga agaacttac 
      caaaaatgg aatctcgtt ggaccctac cgtggtggt 
      tatgaagcc gcaagaaca gttaccacc acatttaca 
      acatcatca cttaccacc ccatttggc accacattt 
      gggcgtgac attaaccga atcggttaa tcaacaatt 
      tgaccattt tgaacaacc accgcctgc tccctatga 
      ccaacaacc tcatcagta tccaccacc tccgccgca 
      acaacatca agtcgacag tgccgttct acagcaaca 
      acaacacaa ttttgacgt gcaggcgcc cgcagccct 
      gttagatcc tctggatta caggcaaac gccgacctg 
      tctgaagat gcttcccaa gagatatat taacgtgca 
      ccagtggct aggaccggt gtgccccaa ggacctaaa 
      agctcagct tcaactctt ccgatctca acaaaccca 
      agcggcaac tcctagcgg agccgccca attgcagtt 
      gctgcaaca gcaacaaca atatctcgc ccaacagca 
      acaacaagc ggcgtttcc tcaaccacc ttacgttct 
      taaccatca gcaagaacc ctcctacct cattaccgc 
      cggtgttcc acaatatta tggtgttgg tccatgggt 
      atatccggc accggcgaa tctcatcca acaaggagc 
      tagcaacgg agccagaag acctctcac tccaaatgg 
      cacagccga agcacaaca gcaagtaca accgcaagt 
      tcctggggg ttacattgt tccatacta tgatcaaaa 
      taccccaat tcttatggc acaaggtgc ggcaatgcg 
      aaatggtac acccatgcg cttggttag ccctgctcc 
      tgtacttgt gcaacctca agctaaccg acaaacacc 
      agcaggtgc ttccctgta ttctagtac tccccagag 
      tttgtatgg tgctaatgt gaatacttc ggttaatgg 
      aggaacttt aggaggtgt ggcccaagg tgttggttc 
      tgggttatt cccacctct tcatgcagc accaccttc 
      tgctccatt cggtagttc ttctctagg aaatatcgg 
      ttcatccgc tacaaccac atcccttca tacaggcct 
      gggactgaa cacgacatc gtctggacg tcgcgactc 
      gttcgaccg gaacacgtc agcgttctc gccgtcgct 
      ggactacag acagaaatg gccaacatc ctacaacat 
      cggcagcag tcccagtcc tctaacggg aagcctgac 
      acctccgcc gtcggcgcc tctccaact cggcggact 
      gatgacatc gcgggttct ttcggcggc gcccggagc 
      cgaagcgaa ataccgaaa ttccgtggc ggcaggact 
      gacaacgaa tgcgatgtt tggatcgac gaattccct 
      cttcacgaa aataaattc atcgttatc aacggtccc 
      tagttcgct ggataaggg acaacaggg ccgatcacg 
      cctgctcga agactttag aaataacag atatccgaa 
      tttacaatt gagagattt ggccaatca catcgtgga 
      gttctcgca ggaccagca cgggtcgcg gtttatcca 
      acagaagtt agaacgggc ttcggcgac cgaaaaaca 
      gatggtttt taatgaaat tctatcggc agcttataa 
      tcttatgac tgacgtctt cggcaatta tgttataca 
      aaagttctt cgaattcgg gaccgctga acagaagac 
      gacgttggc gcagaaagt cagaggtca cgtgcttcc 
      attagctct acaaatgta tggttgtcg ggtcattca 
      aaaggctct cgaatcgat tccgcctga acaacagca 
      agaaattgt gagagaatt ggacgggca tgttttgaa 
      gtgcgtcaa ggaccaaaa cggaaatca tgtggtcca 
      aaagtgcat cgaatgtgt cgaccccaa cgccttgca 
      gtttattat tcagtcatt ctcggggca agtgtacac 
      gttatcgac ccatccgta cggctgccg ggtcattca 
      acgcatttt agagcattg cacacctga gcagactgc 
      tccaatttt agccgaatt gcaccaaca caccgatca 
      gttgatcca ggatcagtt tggaaacta tgtcatcca 
      gcatgtgtt ggaacacgg aaagcccga agataagag 
      tcagctgat tagcagcgt gagagggaa agttttagc 
      tttaagtca gcataaatt cgccagtaa cgtcgtcga 
      gaagtgcgt cactcacgc gacccgggc cgagagggc 
      gctactaat cgaggaagt gtgtggatt taacgacaa 
      tgctctcca tgtcatgat gaaagatca gtatgcgaa 
      ttacgtggt tcaaaaaat gatagacgt gagtgagcc 
      aacacagcg aaaggtgtt gatgcataa aatcaggcc 
      tcatcttaa ctcgttgag gaaatacac gtacggcaa 
      gcatattat tgccaagtt ggagaaata ttttatgaa 
      gactccggg atctatggg ctctatcgg aggagagct 
      cggtccgat tggaccgcc gaccaatgg agtgctcta 
      atttaagcg atgcttatt cattttaaa tatattttt 
      aattttttt gaccaattg tgtgtgttg attgagttt 
      tgttgacac accaacaag gttccagta tttttcgtt 
      aattttttc tcttcatcg atggaattt tcagttcgt 
      aattttatt tctgacttt atttgtaag gttaatatg 
      ttattgtga atacataca aatatctgt attatagct 
      gtaatttat gtaatttgt tttgcatca agtttcatc 
      gtttcattg tttgacttt tgtttcacg agctgtagc 
      gaagtcgtt cttaagttt tttatatat agatattta 
      atttatttt gctttttta cgttcgggg tgagcctgt 
      aaaacaaaa ggcaaccaa tttcatcat gtgcatgtt 
      taatttgtg tctgaaaca gttgtaatt aattattga 
      gcagagttc tgaaacgaa tctcaaatt ttattttag 
      ttcagttcg aaatggatg tcttgtaaa atttcccct 
      aaaattaaa ccgaatacc tgtacataa gaactaaac 
      agtttcatc atggaatac taagtgtcc cccaaatta 
      tcccaacag tatgttaaa aatgtgata aggtcctca 
      ggaaataga tttgacatc attcaagtt catccaaat 
      caaaatttt tctagttcc gcacattac acgttatca 
      cattcaacc taacacatt tgaagcaat cgcttgaat 
      ttattaaaa ttttaggcc gtattgact caatttttg 
      acgtttaag atcaaaaac aatgttgga atatctacg 
      tagggtagg taaagtgct ttgaattgt agcgatttt 
      aaacttttg ggccaatac ggcggctgc taacggcat 
      ttaatgcac acaatattt gcatttatg gtagtttag 
      tcatgtttg atgtagtat ctcacgaaa aaccttatc 
      ttgaataga aaaatcggt cgattattt cttcaaagt 
      tcaaatgtg actcgtttc gtttaaatg actattcaa 
      ctctagttt cgaatacat tttctcata agta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY