General information | |||
TC005070 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7132460..7134606 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005070 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03897 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
Comment: Head pronotum curled inwards. |
elytra dorsal orientation not closed - Number of animals showing the phenotype: 6
elytra surface irregular - Number of animals showing the phenotype: 6
thorax orientation ventralized - Number of animals showing the phenotype: 2
Comment: Head pronotum curled inwards. |
- >TC005070 RA
MDIGELLDY KPPQGVKRP VENENDEIE KQRKMRRIA RDKANRMSR AASPTPSIQ TLDQISEEE RQEILKFVE TEQTEGEVI DEAAIKRIV LNFEKRSLK NREMRIKFP DSPEKFMES ELELHEILQ EMRVLSTAP DYYPLLVKL QVIPSLLEL LAHDNTDVA VATVELLQE LTDVDILHE SEEGAEALI DALLDHQVI ALLVQNLDR LDEKVKEEA DGVHNSLSI IENLTELRN DICNEATKQ GLLQWLLKR LKVKAPFDA NKLYASELL SILLQDNEK NRTALGEMD GIDTLLQQL AFYKRHDPS SAEEHELME NLFNCLCSA LMIVSNRDR FLKGEGLQL MNLMLREKK TSRNGSLKV LDYAMSGPH GKDNCNKFV DILGLRTIF PLFMKTPKK NRKKVLSTE EHEEHVTSI IASMLRNCR GSQRQRLIS KFTENDHEK VDRLLELHF KYLEKVEAI DEAIEEENG VEDDDASYM KRLEGGLFT LQLVDYIIV EVCAAGPAS IKQRVMQIL NLRGASLKT IRHIMREYA GNLGDEGDK EWRDQEQQH ILHLVDKF
- >TC005070 RA
atggatatt ggtgaatta ctggactac aaaccccct caaggcgta aaacgccca gtcgagaat gaaaacgat gaaatcgaa aaacagaga aaaatgcgg cgaattgca cgggataaa gccaataga atgtcccgt gccgcatcc ccaaccccg tcaatacaa actttggac cagatatcc gaagaagaa agacaagaa atattaaaa tttgtggaa actgaacaa acagaaggc gaagtcatt gacgaagcg gcaatcaag cgaattgtt ctaaatttc gaaaaacgg agtctcaaa aacagagaa atgaggatc aagtttcca gactcgcct gaaaagttt atggagagc gagctcgaa ctacatgaa attttacaa gaaatgagg gtgttatcg accgcacct gactattac ccccttctt gtgaagctg caagtcata cccagcttg ctggaactg ttggcacat gataacact gatgtggca gtggcgacc gtggagctg ttgcaagaa ctgaccgat gtggacatc ctccacgag tctgaagaa ggtgccgaa gccctaatt gacgccttg ctcgaccac caagtgata gccctcctc gtccaaaac ctggaccgt ttggacgaa aaagtgaag gaggaagca gacggtgtc cacaactcc ctatcaata atagaaaac ttgaccgaa ctccgcaat gacatttgc aacgaggcc accaaacag ggcttgctc cagtggctc ttgaaacgg ttgaaagtc aaagcccct ttcgacgcc aacaaactc tacgccagc gaactcctc tcaatcctg ctccaagac aacgaaaaa aaccgaact gctttgggc gaaatggac ggaatcgac actttgttg caacagttg gccttctac aagcgccac gacccctcc agtgccgaa gaacacgag ctaatggag aacctgttc aactgtttg tgcagcgca ctgatgatc gtctcaaac cgagaccgg tttctcaaa ggcgagggc ttgcaactg atgaatttg atgctcaga gagaagaaa acctcgcgg aacggctca cttaaagtg ctggattac gcaatgtcg ggcccccat ggcaaagac aattgcaac aagttcgtg gatatttta ggcctgcgg accattttc cccctattt atgaaaacg ccgaagaag aacaggaag aaagtcttg tcgacggaa gagcacgaa gagcacgtc acttcgatt atcgcgtcc atgctgcgg aattgtcgc gggtcgcag agacaaagg ctcattagt aaatttacg gaaaatgat cacgagaaa gtcgacagg ttgcttgag ctacacttc aaatatttg gagaaggtt gaagccatt gatgaggcc attgaggag gaaaatgga gttgaggat gatgatgcg agttatatg aagcggctg gaaggagga ttgttcacg ttgcagttg gtggattat attattgtg gaagtttgt gctgcggga cccgccagt attaagcag cgagttatg caaatcctc aatcttagg ggggcttcg ttgaagacg ataaggcac attatgcgg gaatatgcc gggaatttg ggggacgaa ggggataag gagtggagg gatcaggag cagcaacac attttgcac ttagttgat aagttttga
- >TC005070 RA
ataggttaa attcacaga aatttgaca taaacaaag aggttagtt attaagttt cccaaaaaa ccttaaaaa tggatattg gtgaattac tggactaca aaccccctc aaggcgtaa aacgcccag tcgagaatg aaaacgatg aaatcgaaa aacagagaa aaatgcggc gaattgcac gggataaag ccaatagaa tgtcccgtg ccgcatccc caaccccgt caatacaaa ctttggacc agatatccg aagaagaaa gacaagaaa tattaaaat ttgtggaaa ctgaacaaa cagaaggcg aagtcattg acgaagcgg caatcaagc gaattgttc taaatttcg aaaaacgga gtctcaaaa acagagaaa tgaggatca agtttccag actcgcctg aaaagttta tggagagcg agctcgaac tacatgaaa ttttacaag aaatgaggg tgttatcga ccgcacctg actattacc cccttcttg tgaagctgc aagtcatac ccagcttgc tggaactgt tggcacatg ataacactg atgtggcag tggcgaccg tggagctgt tgcaagaac tgaccgatg tggacatcc tccacgagt ctgaagaag gtgccgaag ccctaattg acgccttgc tcgaccacc aagtgatag ccctcctcg tccaaaacc tggaccgtt tggacgaaa aagtgaagg aggaagcag acggtgtcc acaactccc tatcaataa tagaaaact tgaccgaac tccgcaatg acatttgca acgaggcca ccaaacagg gcttgctcc agtggctct tgaaacggt tgaaagtca aagcccctt tcgacgcca acaaactct acgccagcg aactcctct caatcctgc tccaagaca acgaaaaaa accgaactg ctttgggcg aaatggacg gaatcgaca ctttgttgc aacagttgg ccttctaca agcgccacg acccctcca gtgccgaag aacacgagc taatggaga acctgttca actgtttgt gcagcgcac tgatgatcg tctcaaacc gagaccggt ttctcaaag gcgagggct tgcaactga tgaatttga tgctcagag agaagaaaa cctcgcgga acggctcac ttaaagtgc tggattacg caatgtcgg gcccccatg gcaaagaca attgcaaca agttcgtgg atattttag gcctgcgga ccattttcc ccctattta tgaaaacgc cgaagaaga acaggaaga aagtcttgt cgacggaag agcacgaag agcacgtca cttcgatta tcgcgtcca tgctgcgga attgtcgcg ggtcgcaga gacaaaggc tcattagta aatttacgg aaaatgatc acgagaaag tcgacaggt tgcttgagc tacacttca aatatttgg agaaggttg aagccattg atgaggcca ttgaggagg aaaatggag ttgaggatg atgatgcga gttatatga agcggctgg aaggaggat tgttcacgt tgcagttgg tggattata ttattgtgg aagtttgtg ctgcgggac ccgccagta ttaagcagc gagttatgc aaatcctca atcttaggg gggcttcgt tgaagacga taaggcaca ttatgcggg aatatgccg ggaatttgg gggacgaag gggataagg agtggaggg atcaggagc agcaacaca ttttgcact tagttgata agttttgat atgttattg ccattactt cgaataaaa attatttca tttggtatt ttcctaatt tctcgtcac aagggattt tgcagtgtt caaaaatta taatcactt tattaacag ccaacggat cttttaaaa acaaagaca ctacacttg actcaagta caaacacta aaacacttc tttctaaga ctaaaaata agtaataaa cgtaacaaa ataag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
