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Gene information
General information
TC005070 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7132460..7134606
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005070
    iBeetle screen iB_03897

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    Adult morphology
    head orientation ventralized - Number of animals showing the phenotype: 2

    Comment: Head pronotum curled inwards.

    elytra dorsal orientation not closed - Number of animals showing the phenotype: 6


    elytra surface irregular - Number of animals showing the phenotype: 6


    thorax orientation ventralized - Number of animals showing the phenotype: 2
    Comment: Head pronotum curled inwards.

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005070 RA
      MDIGELLDY KPPQGVKRP VENENDEIE KQRKMRRIA 
      RDKANRMSR AASPTPSIQ TLDQISEEE RQEILKFVE 
      TEQTEGEVI DEAAIKRIV LNFEKRSLK NREMRIKFP 
      DSPEKFMES ELELHEILQ EMRVLSTAP DYYPLLVKL 
      QVIPSLLEL LAHDNTDVA VATVELLQE LTDVDILHE 
      SEEGAEALI DALLDHQVI ALLVQNLDR LDEKVKEEA 
      DGVHNSLSI IENLTELRN DICNEATKQ GLLQWLLKR 
      LKVKAPFDA NKLYASELL SILLQDNEK NRTALGEMD 
      GIDTLLQQL AFYKRHDPS SAEEHELME NLFNCLCSA 
      LMIVSNRDR FLKGEGLQL MNLMLREKK TSRNGSLKV 
      LDYAMSGPH GKDNCNKFV DILGLRTIF PLFMKTPKK 
      NRKKVLSTE EHEEHVTSI IASMLRNCR GSQRQRLIS 
      KFTENDHEK VDRLLELHF KYLEKVEAI DEAIEEENG 
      VEDDDASYM KRLEGGLFT LQLVDYIIV EVCAAGPAS 
      IKQRVMQIL NLRGASLKT IRHIMREYA GNLGDEGDK 
      EWRDQEQQH ILHLVDKF
    CDS sequence
    • >TC005070 RA
      atggatatt ggtgaatta ctggactac aaaccccct 
      caaggcgta aaacgccca gtcgagaat gaaaacgat 
      gaaatcgaa aaacagaga aaaatgcgg cgaattgca 
      cgggataaa gccaataga atgtcccgt gccgcatcc 
      ccaaccccg tcaatacaa actttggac cagatatcc 
      gaagaagaa agacaagaa atattaaaa tttgtggaa 
      actgaacaa acagaaggc gaagtcatt gacgaagcg 
      gcaatcaag cgaattgtt ctaaatttc gaaaaacgg 
      agtctcaaa aacagagaa atgaggatc aagtttcca 
      gactcgcct gaaaagttt atggagagc gagctcgaa 
      ctacatgaa attttacaa gaaatgagg gtgttatcg 
      accgcacct gactattac ccccttctt gtgaagctg 
      caagtcata cccagcttg ctggaactg ttggcacat 
      gataacact gatgtggca gtggcgacc gtggagctg 
      ttgcaagaa ctgaccgat gtggacatc ctccacgag 
      tctgaagaa ggtgccgaa gccctaatt gacgccttg 
      ctcgaccac caagtgata gccctcctc gtccaaaac 
      ctggaccgt ttggacgaa aaagtgaag gaggaagca 
      gacggtgtc cacaactcc ctatcaata atagaaaac 
      ttgaccgaa ctccgcaat gacatttgc aacgaggcc 
      accaaacag ggcttgctc cagtggctc ttgaaacgg 
      ttgaaagtc aaagcccct ttcgacgcc aacaaactc 
      tacgccagc gaactcctc tcaatcctg ctccaagac 
      aacgaaaaa aaccgaact gctttgggc gaaatggac 
      ggaatcgac actttgttg caacagttg gccttctac 
      aagcgccac gacccctcc agtgccgaa gaacacgag 
      ctaatggag aacctgttc aactgtttg tgcagcgca 
      ctgatgatc gtctcaaac cgagaccgg tttctcaaa 
      ggcgagggc ttgcaactg atgaatttg atgctcaga 
      gagaagaaa acctcgcgg aacggctca cttaaagtg 
      ctggattac gcaatgtcg ggcccccat ggcaaagac 
      aattgcaac aagttcgtg gatatttta ggcctgcgg 
      accattttc cccctattt atgaaaacg ccgaagaag 
      aacaggaag aaagtcttg tcgacggaa gagcacgaa 
      gagcacgtc acttcgatt atcgcgtcc atgctgcgg 
      aattgtcgc gggtcgcag agacaaagg ctcattagt 
      aaatttacg gaaaatgat cacgagaaa gtcgacagg 
      ttgcttgag ctacacttc aaatatttg gagaaggtt 
      gaagccatt gatgaggcc attgaggag gaaaatgga 
      gttgaggat gatgatgcg agttatatg aagcggctg 
      gaaggagga ttgttcacg ttgcagttg gtggattat 
      attattgtg gaagtttgt gctgcggga cccgccagt 
      attaagcag cgagttatg caaatcctc aatcttagg 
      ggggcttcg ttgaagacg ataaggcac attatgcgg 
      gaatatgcc gggaatttg ggggacgaa ggggataag 
      gagtggagg gatcaggag cagcaacac attttgcac 
      ttagttgat aagttttga
    mRNA sequence
    • >TC005070 RA
      ataggttaa attcacaga aatttgaca taaacaaag 
      aggttagtt attaagttt cccaaaaaa ccttaaaaa 
      tggatattg gtgaattac tggactaca aaccccctc 
      aaggcgtaa aacgcccag tcgagaatg aaaacgatg 
      aaatcgaaa aacagagaa aaatgcggc gaattgcac 
      gggataaag ccaatagaa tgtcccgtg ccgcatccc 
      caaccccgt caatacaaa ctttggacc agatatccg 
      aagaagaaa gacaagaaa tattaaaat ttgtggaaa 
      ctgaacaaa cagaaggcg aagtcattg acgaagcgg 
      caatcaagc gaattgttc taaatttcg aaaaacgga 
      gtctcaaaa acagagaaa tgaggatca agtttccag 
      actcgcctg aaaagttta tggagagcg agctcgaac 
      tacatgaaa ttttacaag aaatgaggg tgttatcga 
      ccgcacctg actattacc cccttcttg tgaagctgc 
      aagtcatac ccagcttgc tggaactgt tggcacatg 
      ataacactg atgtggcag tggcgaccg tggagctgt 
      tgcaagaac tgaccgatg tggacatcc tccacgagt 
      ctgaagaag gtgccgaag ccctaattg acgccttgc 
      tcgaccacc aagtgatag ccctcctcg tccaaaacc 
      tggaccgtt tggacgaaa aagtgaagg aggaagcag 
      acggtgtcc acaactccc tatcaataa tagaaaact 
      tgaccgaac tccgcaatg acatttgca acgaggcca 
      ccaaacagg gcttgctcc agtggctct tgaaacggt 
      tgaaagtca aagcccctt tcgacgcca acaaactct 
      acgccagcg aactcctct caatcctgc tccaagaca 
      acgaaaaaa accgaactg ctttgggcg aaatggacg 
      gaatcgaca ctttgttgc aacagttgg ccttctaca 
      agcgccacg acccctcca gtgccgaag aacacgagc 
      taatggaga acctgttca actgtttgt gcagcgcac 
      tgatgatcg tctcaaacc gagaccggt ttctcaaag 
      gcgagggct tgcaactga tgaatttga tgctcagag 
      agaagaaaa cctcgcgga acggctcac ttaaagtgc 
      tggattacg caatgtcgg gcccccatg gcaaagaca 
      attgcaaca agttcgtgg atattttag gcctgcgga 
      ccattttcc ccctattta tgaaaacgc cgaagaaga 
      acaggaaga aagtcttgt cgacggaag agcacgaag 
      agcacgtca cttcgatta tcgcgtcca tgctgcgga 
      attgtcgcg ggtcgcaga gacaaaggc tcattagta 
      aatttacgg aaaatgatc acgagaaag tcgacaggt 
      tgcttgagc tacacttca aatatttgg agaaggttg 
      aagccattg atgaggcca ttgaggagg aaaatggag 
      ttgaggatg atgatgcga gttatatga agcggctgg 
      aaggaggat tgttcacgt tgcagttgg tggattata 
      ttattgtgg aagtttgtg ctgcgggac ccgccagta 
      ttaagcagc gagttatgc aaatcctca atcttaggg 
      gggcttcgt tgaagacga taaggcaca ttatgcggg 
      aatatgccg ggaatttgg gggacgaag gggataagg 
      agtggaggg atcaggagc agcaacaca ttttgcact 
      tagttgata agttttgat atgttattg ccattactt 
      cgaataaaa attatttca tttggtatt ttcctaatt 
      tctcgtcac aagggattt tgcagtgtt caaaaatta 
      taatcactt tattaacag ccaacggat cttttaaaa 
      acaaagaca ctacacttg actcaagta caaacacta 
      aaacacttc tttctaaga ctaaaaata agtaataaa 
      cgtaacaaa ataag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY