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Gene information
General information
TC004976 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5409457..5431333
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004976
    iBeetle screen iB_03886

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna flagellum shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages shape rounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs pretarsus rounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen posterior circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004976 RA
      MFWNRCFVL ISFTWIVGV ECQLSDNTP PTMWLDRNW 
      QIPESEPVG SKIIQAHGR DNEGGRVIY GLEPLQSLG 
      NEKPTEKLP FRIDPYTGI VYLNESLQG RGGQNVRLY 
      VTASDGELA AKFEVFVNI LKDSDPNSS LNFPGSKQN 
      GLVPPPFLT IPNFNQRPK SPTTATSRQ PHFQPKPNV 
      TTLLRPIKI EPVKNEIDE KEEATTRKI TKTTPTVQP 
      LNKTIDQAV KTPPDLAVT VVPIISVCA VFLMVGIIA 
      IVFRKKIHL GKPKSSKDD IRKPSSGAI VLPEDTTIG 
      MQQWRGPTA FNNRYELWE REVNRSQAS HQISAPIKD 
      SDHWEFPRH RLKFFNILG EGAFGQVWK CEASDIDGK 
      EGVSVVAVK TLKENANEK EKSDLLSEL RVMKMLETH 
      PNVVRLLGC CTDKEPIFL IMEYISKGK LQSYLRNSR 
      AERYYNNMH GQSKSLTSR DLTSFVYQV AKGMEFLSA 
      NGIIHRDLA ARNILITEE HTCKVADFG FARDVIVSH 
      VYERKSEGR LPIRWMAPE SLYDNIFSV KSDVWSFGV 
      LIWEVVTLG STPYPGLSA AEVMKKVRD GYRLDKPEH 
      CRRELYNIM YYCWDKDPK QRPSFGECV QLLEKLLMT 
      ETDYIELER FPDHSYYNM MSLSGEKL
    • >TC004976 RB
      MFWNRCFVL ISFTWIVGE CQLSDNTPP TMWLDRNWQ 
      IPESEPVGS KIIQAHGRD NEGGRVIYG LEPLQSLGN 
      EKPTEKLPF RIDPYTGIV YLNESLQGR GGQNVRLYV 
      TASDGELAA KFEVFVNIL KDSDPNSSL NFPGSKQNG 
      LVPPPFLTI PNFNQRPKS PTTATSRQP HFQPKPNVT 
      TLLRPIKIE PVKNEIDEK EEATTRKIT KTTPTVQPL 
      NKTIDQAVK TPPDLAVTV VPIISVCAV FLMVGIIAI 
      VFRKKIHLG KPKSSKDDI RKPSSGAIV LPEDTTIGM 
      QQWRGPTAF NNRYELWER EVNRSQASH QISAPIKDS 
      DHWEFPRHR LKFFNILGE GAFGQVWKC EASDIDGKE 
      GVSVVAVKT LKENANEKE KSDLLSELR VMKMLETHP 
      NVVRLLGCC TDKEPIFLI MEYISKGKL QSYLRNSRA 
      ERYYNNMHG QSKSLTSRD LTSFVYQVA KGMEFLSAN 
      GIIHRDLAA RNILITEEH TCKVADFGF ARDVIVSHV 
      YERKSEGRL PIRWMAPES LYDNIFSVK SDVWSFGVL 
      IWEVVTLGS TPYPGLSAA EVMKKVRDG YRLDKPEHC 
      RRELYNIMY YCWDKDPKQ RPSFGECVQ LLEKLLMTE 
      TDYIELERF PDHSYYNMM SLSGEKL
    CDS sequence
    • >TC004976 RA
      atgttttgg aatcgttgc tttgttcta atttctttc 
      acctggatc gtcggagta gaatgccaa ctcagcgac 
      aacactcct cccaccatg tggttagac cggaactgg 
      caaattccc gaatcggaa cctgtggga agcaaaata 
      atccaggcg cacggtaga gacaatgag ggagggcgc 
      gttatttac ggcctagag cccctccag tctttaggg 
      aacgaaaaa ccaaccgag aaactacct ttccggata 
      gatccttac acgggaatc gtctacttg aacgaatcc 
      ttgcaagga cgaggaggt cagaacgtg cggctgtat 
      gtcacagcg tctgatggc gaactagcg gccaagttt 
      gaggttttt gtcaacatt ttgaaggat tcggacccg 
      aattcgagc cttaatttc ccgggaagt aaacaaaat 
      gggttggta ccgccgcct tttctcact attccgaat 
      tttaaccaa cgtccgaaa agccccaca acggctacg 
      tcaagacaa ccgcacttt caaccaaaa ccaaacgtt 
      acgaccctt ctaagaccg attaaaatc gaaccagtt 
      aaaaatgaa attgatgaa aaagaggaa gcaacgacg 
      aggaaaatc actaagacg acaccgaca gttcaaccc 
      cttaataaa actatagac caagcggtt aaaacacca 
      cctgatttg gccgttact gttgtacca attatctca 
      gtttgtgct gtgtttctc atggttggc atcattgcc 
      atagttttt aggaagaaa atacattta gggaaaccc 
      aaaagttcc aaagacgat attaggaaa ccatcttca 
      ggtgcaatt gttctacca gaagacaca actattggt 
      atgcaacaa tggagagga cccacagct ttcaacaat 
      cgctacgaa ctgtgggaa cgtgaagta aaccgttca 
      caggccagt catcaaatt tccgcccca atcaaggac 
      tccgaccac tgggagttc ccccgacac cgtttgaaa 
      tttttcaat atattgggc gagggagca ttcggccaa 
      gtgtggaaa tgcgaagct tccgatatt gacggtaag 
      gagggcgtt tccgtggta gcggtgaaa accctgaaa 
      gagaacgcg aacgagaag gagaaatca gatttgctt 
      tcggagctt cgcgtgatg aaaatgctg gaaactcat 
      ccaaatgtc gtgcgactt ttgggctgt tgtacggac 
      aaagagccg atatttttg attatggaa tatatcagt 
      aaaggcaaa ctgcaaagt tatttacga aattcacga 
      gcagagagg tattacaac aacatgcac ggccaaagc 
      aaatcgcta acgtcgagg gatctcaca tcttttgtg 
      tatcaggtc gccaaggga atggaattt ctgtccgct 
      aatgggata atccatcgg gaccttgcc gcccgcaac 
      atcctcatt acggaggag catacgtgc aaagtggcc 
      gactttggc tttgctaga gacgttata gtctcacac 
      gtttacgaa agaaagagc gaaggaaga ctcccgatc 
      cggtggatg gcccctgag tcactttac gataacatt 
      ttttcggtc aagtcggac gtgtggagc ttcggggtg 
      ctaatttgg gaggtggta acactgggc agcaccccc 
      tacccgggg ctatccgcc gccgaagtc atgaaaaag 
      gtccgagat ggctacaga ttggacaag ccggagcac 
      tgccgtcgt gaactttac aacataatg tattattgt 
      tgggacaaa gacccgaaa cagcggccg tcgtttgga 
      gaatgcgtc cagctgctc gagaagctt ctaatgaca 
      gaaaccgat tatatcgaa ttagaacgc ttccctgat 
      cattcctat tacaacatg atgagtttg agtggcgaa 
      aaactctaa
    • >TC004976 RB
      atgttttgg aatcgttgc tttgttcta atttctttc 
      acctggatc gtcggagaa tgccaactc agcgacaac 
      actcctccc accatgtgg ttagaccgg aactggcaa 
      attcccgaa tcggaacct gtgggaagc aaaataatc 
      caggcgcac ggtagagac aatgaggga gggcgcgtt 
      atttacggc ctagagccc ctccagtct ttagggaac 
      gaaaaacca accgagaaa ctacctttc cggatagat 
      ccttacacg ggaatcgtc tacttgaac gaatccttg 
      caaggacga ggaggtcag aacgtgcgg ctgtatgtc 
      acagcgtct gatggcgaa ctagcggcc aagtttgag 
      gtttttgtc aacattttg aaggattcg gacccgaat 
      tcgagcctt aatttcccg ggaagtaaa caaaatggg 
      ttggtaccg ccgcctttt ctcactatt ccgaatttt 
      aaccaacgt ccgaaaagc cccacaacg gctacgtca 
      agacaaccg cactttcaa ccaaaacca aacgttacg 
      acccttcta agaccgatt aaaatcgaa ccagttaaa 
      aatgaaatt gatgaaaaa gaggaagca acgacgagg 
      aaaatcact aagacgaca ccgacagtt caacccctt 
      aataaaact atagaccaa gcggttaaa acaccacct 
      gatttggcc gttactgtt gtaccaatt atctcagtt 
      tgtgctgtg tttctcatg gttggcatc attgccata 
      gtttttagg aagaaaata catttaggg aaacccaaa 
      agttccaaa gacgatatt aggaaacca tcttcaggt 
      gcaattgtt ctaccagaa gacacaact attggtatg 
      caacaatgg agaggaccc acagctttc aacaatcgc 
      tacgaactg tgggaacgt gaagtaaac cgttcacag 
      gccagtcat caaatttcc gccccaatc aaggactcc 
      gaccactgg gagttcccc cgacaccgt ttgaaattt 
      ttcaatata ttgggcgag ggagcattc ggccaagtg 
      tggaaatgc gaagcttcc gatattgac ggtaaggag 
      ggcgtttcc gtggtagcg gtgaaaacc ctgaaagag 
      aacgcgaac gagaaggag aaatcagat ttgctttcg 
      gagcttcgc gtgatgaaa atgctggaa actcatcca 
      aatgtcgtg cgacttttg ggctgttgt acggacaaa 
      gagccgata tttttgatt atggaatat atcagtaaa 
      ggcaaactg caaagttat ttacgaaat tcacgagca 
      gagaggtat tacaacaac atgcacggc caaagcaaa 
      tcgctaacg tcgagggat ctcacatct tttgtgtat 
      caggtcgcc aagggaatg gaatttctg tccgctaat 
      gggataatc catcgggac cttgccgcc cgcaacatc 
      ctcattacg gaggagcat acgtgcaaa gtggccgac 
      tttggcttt gctagagac gttatagtc tcacacgtt 
      tacgaaaga aagagcgaa ggaagactc ccgatccgg 
      tggatggcc cctgagtca ctttacgat aacattttt 
      tcggtcaag tcggacgtg tggagcttc ggggtgcta 
      atttgggag gtggtaaca ctgggcagc accccctac 
      ccggggcta tccgccgcc gaagtcatg aaaaaggtc 
      cgagatggc tacagattg gacaagccg gagcactgc 
      cgtcgtgaa ctttacaac ataatgtat tattgttgg 
      gacaaagac ccgaaacag cggccgtcg tttggagaa 
      tgcgtccag ctgctcgag aagcttcta atgacagaa 
      accgattat atcgaatta gaacgcttc cctgatcat 
      tcctattac aacatgatg agtttgagt ggcgaaaaa 
      ctctaa
    mRNA sequence
    • >TC004976 RA
      cgcgtttga atcgagcgc ttcgcagag tcccgttaa 
      tttatttta actgaatcg aactttcta aagagatac 
      ctgtcgtgg tctcacatg gcctactcc attcagtat 
      tcccccgtt cgccatccg actacaacc gattaaaaa 
      tcgcgcaaa atgaaggaa aaaggttga ttttcgcga 
      atgtgtgaa aatccgtgt gaaccccag tgtcagtgc 
      gggaaaatg tgagtggtt tgggagatt tcaacgcac 
      gatcaagcg aaaaatgta gactacata ccaagtgaa 
      gtgacttaa aattgataa acaatgttt tggaatcgt 
      tgctttgtt ctaatttct ttcacctgg atcgtcgga 
      gtagaatgc caactcagc gacaacact cctcccacc 
      atgtggtta gaccggaac tggcaaatt cccgaatcg 
      gaacctgtg ggaagcaaa ataatccag gcgcacggt 
      agagacaat gagggaggg cgcgttatt tacggccta 
      gagcccctc cagtcttta gggaacgaa aaaccaacc 
      gagaaacta cctttccgg atagatcct tacacggga 
      atcgtctac ttgaacgaa tccttgcaa ggacgagga 
      ggtcagaac gtgcggctg tatgtcaca gcgtctgat 
      ggcgaacta gcggccaag tttgaggtt tttgtcaac 
      attttgaag gattcggac ccgaattcg agccttaat 
      ttcccggga agtaaacaa aatgggttg gtaccgccg 
      ccttttctc actattccg aattttaac caacgtccg 
      aaaagcccc acaacggct acgtcaaga caaccgcac 
      tttcaacca aaaccaaac gttacgacc cttctaaga 
      ccgattaaa atcgaacca gttaaaaat gaaattgat 
      gaaaaagag gaagcaacg acgaggaaa atcactaag 
      acgacaccg acagttcaa ccccttaat aaaactata 
      gaccaagcg gttaaaaca ccacctgat ttggccgtt 
      actgttgta ccaattatc tcagtttgt gctgtgttt 
      ctcatggtt ggcatcatt gccatagtt tttaggaag 
      aaaatacat ttagggaaa cccaaaagt tccaaagac 
      gatattagg aaaccatct tcaggtgca attgttcta 
      ccagaagac acaactatt ggtatgcaa caatggaga 
      ggacccaca gctttcaac aatcgctac gaactgtgg 
      gaacgtgaa gtaaaccgt tcacaggcc agtcatcaa 
      atttccgcc ccaatcaag gactccgac cactgggag 
      ttcccccga caccgtttg aaatttttc aatatattg 
      ggcgaggga gcattcggc caagtgtgg aaatgcgaa 
      gcttccgat attgacggt aaggagggc gtttccgtg 
      gtagcggtg aaaaccctg aaagagaac gcgaacgag 
      aaggagaaa tcagatttg ctttcggag cttcgcgtg 
      atgaaaatg ctggaaact catccaaat gtcgtgcga 
      cttttgggc tgttgtacg gacaaagag ccgatattt 
      ttgattatg gaatatatc agtaaaggc aaactgcaa 
      agttattta cgaaattca cgagcagag aggtattac 
      aacaacatg cacggccaa agcaaatcg ctaacgtcg 
      agggatctc acatctttt gtgtatcag gtcgccaag 
      ggaatggaa tttctgtcc gctaatggg ataatccat 
      cgggacctt gccgcccgc aacatcctc attacggag 
      gagcatacg tgcaaagtg gccgacttt ggctttgct 
      agagacgtt atagtctca cacgtttac gaaagaaag 
      agcgaagga agactcccg atccggtgg atggcccct 
      gagtcactt tacgataac attttttcg gtcaagtcg 
      gacgtgtgg agcttcggg gtgctaatt tgggaggtg 
      gtaacactg ggcagcacc ccctacccg gggctatcc 
      gccgccgaa gtcatgaaa aaggtccga gatggctac 
      agattggac aagccggag cactgccgt cgtgaactt 
      tacaacata atgtattat tgttgggac aaagacccg 
      aaacagcgg ccgtcgttt ggagaatgc gtccagctg 
      ctcgagaag cttctaatg acagaaacc gattatatc 
      gaattagaa cgcttccct gatcattcc tattacaac 
      atgatgagt ttgagtggc gaaaaactc taacgtttg 
      cacaaagtt ttgttgtta taagttaag ctcactccc 
      agtggttgt tacaaagaa ataaccaaa gttatgaca 
      aacagtttt tcacgcggt ttaaattat ttatggtag 
      ctttagtga caagactgt aagttagtt aagttcgag 
      ttagtttaa ttgtaattg tgtgctcca actttatta 
      ttgtattaa tcctgagaa gggcacttt cagcccaat 
      taaagtgat gcgaacaaa gaagggcgg taagtacct 
      gcaagtccg gtgtcgtta acttaactc actaaatta 
      taattttta ttttattac attgtgcaa taaaaacga 
      aaacattta
    • >TC004976 RB
      cgcgtttga atcgagcgc ttcgcagag tcccgttaa 
      tttatttta actgaatcg aactttcta aagagatac 
      ctgtcgtgg tctcacatg gcctactcc attcagtat 
      tcccccgtt cgccatccg actacaacc gattaaaaa 
      tcgcgcaaa atgaaggaa aaaggttga ttttcgcga 
      atgtgtgaa aatccgtgt gaaccccag tgtcagtgc 
      gggaaaatg tgagtggtt tgggagatt tcaacgcac 
      gatcaagcg aaaaatgta gactacata ccaagtgaa 
      gtgacttaa aattgataa acaatgttt tggaatcgt 
      tgctttgtt ctaatttct ttcacctgg atcgtcgga 
      gaatgccaa ctcagcgac aacactcct cccaccatg 
      tggttagac cggaactgg caaattccc gaatcggaa 
      cctgtggga agcaaaata atccaggcg cacggtaga 
      gacaatgag ggagggcgc gttatttac ggcctagag 
      cccctccag tctttaggg aacgaaaaa ccaaccgag 
      aaactacct ttccggata gatccttac acgggaatc 
      gtctacttg aacgaatcc ttgcaagga cgaggaggt 
      cagaacgtg cggctgtat gtcacagcg tctgatggc 
      gaactagcg gccaagttt gaggttttt gtcaacatt 
      ttgaaggat tcggacccg aattcgagc cttaatttc 
      ccgggaagt aaacaaaat gggttggta ccgccgcct 
      tttctcact attccgaat tttaaccaa cgtccgaaa 
      agccccaca acggctacg tcaagacaa ccgcacttt 
      caaccaaaa ccaaacgtt acgaccctt ctaagaccg 
      attaaaatc gaaccagtt aaaaatgaa attgatgaa 
      aaagaggaa gcaacgacg aggaaaatc actaagacg 
      acaccgaca gttcaaccc cttaataaa actatagac 
      caagcggtt aaaacacca cctgatttg gccgttact 
      gttgtacca attatctca gtttgtgct gtgtttctc 
      atggttggc atcattgcc atagttttt aggaagaaa 
      atacattta gggaaaccc aaaagttcc aaagacgat 
      attaggaaa ccatcttca ggtgcaatt gttctacca 
      gaagacaca actattggt atgcaacaa tggagagga 
      cccacagct ttcaacaat cgctacgaa ctgtgggaa 
      cgtgaagta aaccgttca caggccagt catcaaatt 
      tccgcccca atcaaggac tccgaccac tgggagttc 
      ccccgacac cgtttgaaa tttttcaat atattgggc 
      gagggagca ttcggccaa gtgtggaaa tgcgaagct 
      tccgatatt gacggtaag gagggcgtt tccgtggta 
      gcggtgaaa accctgaaa gagaacgcg aacgagaag 
      gagaaatca gatttgctt tcggagctt cgcgtgatg 
      aaaatgctg gaaactcat ccaaatgtc gtgcgactt 
      ttgggctgt tgtacggac aaagagccg atatttttg 
      attatggaa tatatcagt aaaggcaaa ctgcaaagt 
      tatttacga aattcacga gcagagagg tattacaac 
      aacatgcac ggccaaagc aaatcgcta acgtcgagg 
      gatctcaca tcttttgtg tatcaggtc gccaaggga 
      atggaattt ctgtccgct aatgggata atccatcgg 
      gaccttgcc gcccgcaac atcctcatt acggaggag 
      catacgtgc aaagtggcc gactttggc tttgctaga 
      gacgttata gtctcacac gtttacgaa agaaagagc 
      gaaggaaga ctcccgatc cggtggatg gcccctgag 
      tcactttac gataacatt ttttcggtc aagtcggac 
      gtgtggagc ttcggggtg ctaatttgg gaggtggta 
      acactgggc agcaccccc tacccgggg ctatccgcc 
      gccgaagtc atgaaaaag gtccgagat ggctacaga 
      ttggacaag ccggagcac tgccgtcgt gaactttac 
      aacataatg tattattgt tgggacaaa gacccgaaa 
      cagcggccg tcgtttgga gaatgcgtc cagctgctc 
      gagaagctt ctaatgaca gaaaccgat tatatcgaa 
      ttagaacgc ttccctgat cattcctat tacaacatg 
      atgagtttg agtggcgaa aaactctaa cgtttgcac 
      aaagttttg ttgttataa gttaagctc actcccagt 
      ggttgttac aaagaaata accaaagtt atgacaaac 
      agtttttca cgcggttta aattattta tggtagctt 
      tagtgacaa gactgtaag ttagttaag ttcgagtta 
      gtttaattg taattgtgt gctccaact ttattattg 
      tattaatcc tgagaaggg cactttcag cccaattaa 
      agtgatgcg aacaaagaa gggcggtaa gtacctgca 
      agtccggtg tcgttaact taactcact aaattataa 
      tttttattt tattacatt gtgcaataa aaacgaaaa 
      cattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY