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Gene information
General information
TC004969 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 3283822..3285554
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004969
    iBeetle screen iB_03884
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    tropharium size shortened - Number of animals showing the phenotype: 4

    egg chamber mostly number not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004969 RA
      MALYDNKYW LLSHIRNSF ISTDDTGMC ELIMTGEEG 
      VRKHLKQSL DSYPDDEDS EDEDDLSHE SYDLQLDTD 
      FPLRYRSNT AVRLEKLEQ VKKRTGRIK HIKWETNKN 
      ELSEEQIDQ LFVKKEPKV IEPKQSLFS KTFKEHKMM 
      PINPYMDYA KFDGSGQVN LQVKKYRIF LTMLPEHQR 
      NYPLAVCCT SSAKIQELI GLILLKCST MYEDYPWKP 
      VNNYGLYIT EEDGEVDRD FPNLDPREN IAKFGFACL 
      GLVEHSDPS KCVSFGNSE TVTIRKFDY NDKKGKDSS 
      KQDQEKQIT TDLHLMDVH KKAMEAPLY KSFKVYMIN 
      KMRAKVEIH LGISGEKIE IDPVQKNSK FVLVKQKPI 
      NHDMDMIAW CEPIETTKS NKMTFKIIY SHSFANAEN 
      VSSPTLQSS ASFKSYEFE ADQSIAEEI VNKINLILD 
      LRSSDTRKE YLAAQERKY YKKKALI
    CDS sequence
    • >TC004969 RA
      atggccctt tacgacaac aaatactgg ttattatca 
      cacatacgc aactctttc atttctaca gatgataca 
      ggaatgtgc gagttaata atgacaggg gaggaaggt 
      gtcaggaaa cacctgaag caaagcctt gattcgtac 
      cctgatgac gaagacagt gaagatgaa gatgatttg 
      tctcacgaa tcttacgac ctacaactt gacaccgat 
      ttcccgtta cgatacaga tcgaatact gccgtaaga 
      ttagaaaaa ttagaacag gttaaaaag agaactggc 
      agaatcaaa catattaaa tgggaaacg aacaagaac 
      gagttgagt gaggaacaa attgatcag ttattcgta 
      aaaaaggaa cccaaagta atagaaccg aagcaatct 
      ttgttttct aaaaccttc aaggagcat aagatgatg 
      cccattaac ccttatatg gattatgcc aagtttgat 
      ggcagcgga caagtcaat cttcaagtg aagaagtat 
      agaattttc ctaacaatg ctaccagag caccagagg 
      aattacccc ttggctgtt tgttgcacc tcttctgct 
      aaaattcaa gagttgatt ggacttata ttactcaaa 
      tgcagtaca atgtatgaa gactatcct tggaagccc 
      gtaaacaat tacggtttg tacataact gaagaagac 
      ggagaagtg gatagggac ttccccaac ttggacccg 
      agggaaaat attgccaaa tttgggttt gcttgttta 
      gggcttgtg gaacattca gatccctca aagtgtgtc 
      agttttggg aactctgaa actgttaca atcagaaaa 
      ttcgattac aatgacaaa aaaggaaaa gattcgtct 
      aaacaagac caggaaaaa caaatcacc accgattta 
      cacttaatg gacgttcat aaaaaagca atggaagcc 
      ccactgtac aaatcgttc aaagtctac atgattaat 
      aaaatgcgg gccaaagtt gaaatacat ttaggaata 
      agtggcgaa aaaatcgaa attgaccca gtgcaaaaa 
      aactcgaaa tttgttcta gttaaacaa aaacccata 
      aaccacgat atggacatg atcgcgtgg tgcgaaccc 
      attgaaact accaaaagc aacaaaatg actttcaaa 
      atcatttac agccattcg ttcgcaaac gctgaaaat 
      gtttcaagt ccaacttta caatcttca gctagcttc 
      aaaagttac gaatttgag gcagaccaa tcaattgcc 
      gaagaaatc gtaaataaa ataaacctg attttggat 
      ttgaggtct agtgacaca cggaaggag tatttggca 
      gcgcaagaa cggaaatat tataagaaa aaagcgttg 
      atttaa
    mRNA sequence
    • >TC004969 RA
      ttacatcag acacgatgg ccctttacg acaacaaat 
      actggttat tatcacaca tacgcaact ctttcattt 
      ctacagatg atacaggaa tgtgcgagt taataatga 
      caggggagg aaggtgtca ggaaacacc tgaagcaaa 
      gccttgatt cgtaccctg atgacgaag acagtgaag 
      atgaagatg atttgtctc acgaatctt acgacctac 
      aacttgaca ccgatttcc cgttacgat acagatcga 
      atactgccg taagattag aaaaattag aacaggtta 
      aaaagagaa ctggcagaa tcaaacata ttaaatggg 
      aaacgaaca agaacgagt tgagtgagg aacaaattg 
      atcagttat tcgtaaaaa aggaaccca aagtaatag 
      aaccgaagc aatctttgt tttctaaaa ccttcaagg 
      agcataaga tgatgccca ttaaccctt atatggatt 
      atgccaagt ttgatggca gcggacaag tcaatcttc 
      aagtgaaga agtatagaa ttttcctaa caatgctac 
      cagagcacc agaggaatt accccttgg ctgtttgtt 
      gcacctctt ctgctaaaa ttcaagagt tgattggac 
      ttatattac tcaaatgca gtacaatgt atgaagact 
      atccttgga agcccgtaa acaattacg gtttgtaca 
      taactgaag aagacggag aagtggata gggacttcc 
      ccaacttgg acccgaggg aaaatattg ccaaatttg 
      ggtttgctt gtttagggc ttgtggaac attcagatc 
      cctcaaagt gtgtcagtt ttgggaact ctgaaactg 
      ttacaatca gaaaattcg attacaatg acaaaaaag 
      gaaaagatt cgtctaaac aagaccagg aaaaacaaa 
      tcaccaccg atttacact taatggacg ttcataaaa 
      aagcaatgg aagccccac tgtacaaat cgttcaaag 
      tctacatga ttaataaaa tgcgggcca aagttgaaa 
      tacatttag gaataagtg gcgaaaaaa tcgaaattg 
      acccagtgc aaaaaaact cgaaatttg ttctagtta 
      aacaaaaac ccataaacc acgatatgg acatgatcg 
      cgtggtgcg aacccattg aaactacca aaagcaaca 
      aaatgactt tcaaaatca tttacagcc attcgttcg 
      caaacgctg aaaatgttt caagtccaa ctttacaat 
      cttcagcta gcttcaaaa gttacgaat ttgaggcag 
      accaatcaa ttgccgaag aaatcgtaa ataaaataa 
      acctgattt tggatttga ggtctagtg acacacgga 
      aggagtatt tggcagcgc aagaacgga aatattata 
      agaaaaaag cgttgattt aagtaactt gtatactaa 
      aatataata ataaattca taaattatt t