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Gene information
General information
TC004954 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5007298..5008672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004954
    iBeetle screen iB_03878

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004954 RA
      MNPSTVFKL LKPQNWLWK SVTRSKTIS DFEKQHKTP 
KCVKPTPIP LALYGGKHT VTMIPGSGI GPELMEFVR 
QVFTVAKAP VHFETVTID ENREDNTDL EYAIISIKR 
NGVAIKGNV DTFSADTTV RLSNVAIRN ELDLCVYLM 
RCKSYPGVR AKHENVDIV VVRQNTEGE YCMLEHSIR 
NGTVVENLK IITEENSQR VAKYAFDYA RKNGRKKVT 
TVHKANIMK FSDGLFLQT ARKVAKDYP DIQHNDIIV 
DNCCMQLVT RPQQFEVLL TPNLYGNIV ANVICGLIG 
GPGLVSGRN YGAHYAVFE PGTRNSGRT IANKNIANP 
VAMLNASVD MLYHLGYVQ HAKVIENAI FQAVAKDKV 
HTPDLGGSA SSVDVVKKI LEYI
    CDS sequence
    • >TC004954 RA
      atgaaccca agcacagtt ttcaagtta ttgaaaccc 
caaaactgg ctttggaaa agtgtgaca cgctctaaa 
accatctcc gatttcgag aagcagcac aagactcca 
aagtgcgtc aaacccacg ccgatacct ttggctctt 
tacggcggc aaacacacc gtcactatg atccccgga 
agtgggata gggccggaa ctgatggaa ttcgtccgc 
caagtgttc acagtggcc aaggccccg gtccatttc 
gaaacggtc acaattgac gaaaatcgc gaagacaac 
accgacctg gaatacgct attatttcg atcaaacgc 
aacggagtt gccattaag ggtaacgtg gacacgttc 
agtgcagac accactgtt cgcctaagc aatgtggcc 
atccgtaac gagctggat ttgtgcgtc tatttgatg 
cgttgcaag tcttatcct ggtgtcaga gcaaagcac 
gagaatgtg gacattgtg gtcgtgcgc caaaacacc 
gaaggtgaa tactgcatg cttgagcac agcattaga 
aacggaacg gtcgtcgaa aacctcaaa atcataacg 
gaggagaac tcgcaacgc gtcgccaaa tacgccttc 
gactatgct cgcaagaac ggccggaaa aaagtaaca 
acggtgcac aaagccaac attatgaaa ttctccgac 
gggttgttc ctccaaacg gcgaggaaa gtcgccaag 
gattatcca gacatccag cacaacgat atcatcgtc 
gataattgt tgtatgcag cttgtgacc agaccgcaa 
cagtttgaa gttctttta actccgaat ttgtatggg 
aatatcgtg gctaatgtc atttgtggg ttaattggg 
ggaccgggt cttgtgtct ggacggaac tatggggcc 
cactatgcc gtgtttgag ccagggacc agaaacagt 
ggtcgaaca attgctaat aaaaatatc gcgaatcca 
gtcgcgatg ttgaacgct tctgtggat atgttgtac 
catttgggg tacgtgcag catgcgaag gtgatcgaa 
aatgcgatt tttcaagct gtggctaag gataaggtg 
catacgccg gatttaggg gggagtgcg agtagtgtt 
gatgttgta aagaaaatt ctcgaatat atttga
    mRNA sequence
    • >TC004954 RA
      ttgtcattg tcaaaattt gacaatgaa cccaagcac 
agttttcaa gttattgaa accccaaaa ctggctttg 
gaaaagtgt gacacgctc taaaaccat ctccgattt 
cgagaagca gcacaagac tccaaagtg cgtcaaacc 
cacgccgat acctttggc tctttacgg cggcaaaca 
caccgtcac tatgatccc cggaagtgg gatagggcc 
ggaactgat ggaattcgt ccgccaagt gttcacagt 
ggccaaggc cccggtcca tttcgaaac ggtcacaat 
tgacgaaaa tcgcgaaga caacaccga cctggaata 
cgctattat ttcgatcaa acgcaacgg agttgccat 
taagggtaa cgtggacac gttcagtgc agacaccac 
tgttcgcct aagcaatgt ggccatccg taacgagct 
ggatttgtg cgtctattt gatgcgttg caagtctta 
tcctggtgt cagagcaaa gcacgagaa tgtggacat 
tgtggtcgt gcgccaaaa caccgaagg tgaatactg 
catgcttga gcacagcat tagaaacgg aacggtcgt 
cgaaaacct caaaatcat aacggagga gaactcgca 
acgcgtcgc caaatacgc cttcgacta tgctcgcaa 
gaacggccg gaaaaaagt aacaacggt gcacaaagc 
caacattat gaaattctc cgacgggtt gttcctcca 
aacggcgag gaaagtcgc caaggatta tccagacat 
ccagcacaa cgatatcat cgtcgataa ttgttgtat 
gcagcttgt gaccagacc gcaacagtt tgaagttct 
tttaactcc gaatttgta tgggaatat cgtggctaa 
tgtcatttg tgggttaat tgggggacc gggtcttgt 
gtctggacg gaactatgg ggcccacta tgccgtgtt 
tgagccagg gaccagaaa cagtggtcg aacaattgc 
taataaaaa tatcgcgaa tccagtcgc gatgttgaa 
cgcttctgt ggatatgtt gtaccattt ggggtacgt 
gcagcatgc gaaggtgat cgaaaatgc gatttttca 
agctgtggc taaggataa ggtgcatac gccggattt 
aggggggag tgcgagtag tgttgatgt tgtaaagaa 
aattctcga atatatttg attaaaatg attactgct 
atagtagtt aagtagacc tcaactact tggtggggt 
aatttttag cgttatacg gtgtgttta accaactat 
tttttgata tagatatca ctatagtag atattactg 
ttagcgtga ttagcagtt actattaaa ttttgatag 
acttcgacg aggtaggta acaacaata cagtgatta 
taaatat
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