Status
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Gene information
General information
TC004952 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4988418..4994982
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004952
    iBeetle screen iB_03877

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004952 RA
      MTHAREDFA VMEATVTTF GKQRFVAVS KALALFIFI 
      LFVGCIIGT ALLVYHLSS CQPISKFEE TTLIVYDKH 
      SDLEVTTTS EEVVTESVS TTTEEIKTE ELDVRLPRS 
      VKPHSYAIR LIPFIVEGN FTFHGEVTI LINVTVTTF 
      NVTLHADDL AIDRVNVYD FNNEILSIR QVTNETRRQ 
      FLIIHMNEP LKSGHQYYV SISFKGVLN DLLQGFYRS 
      SYSVNNELR WIATTQFQA TDARKAFPC FDEPALKAR 
      FQISLARLK NMTSISNMP KIGSPEPVK NLPDYFWDH 
      YEESLPMST YLIAFVISD FDCLKNGSF SVWARPSAL 
      SQTKYSLQI GPQILQFYE NFFGIKYPL PKIDMIGLP 
      DFSAGAMEN WGLITYRES VLLYEEKVS SKASLQRIA 
      HVIAHELAH QWFGNLVTP VWWSDLWLN EGFATYVEC 
      LGANAVNPH LKELDQFVI NELHGALVL DALRTSHQI 
      SIKVNNPDE INDIFDRIS YSKGASILR MMQHFLSMR 
      VFQKGLNRY LKSRMYSNA EQDDLWHTL TLQSHEDKV 
      LDQNVTIKE IMDTWTLQT GFPLVTAYR NYENDSVTF 
      TQERFLVND DDQRSKSVL WWIPITYTN PKNVLRSNW 
      MRNEQILTI HELKQPKNH WLLVNVNQT GYYRVNYDP 
      RNWNLIVQQ LLKKNGHLV FDPKNRAQL LDDALHLAS 
      VGYLDYNIA LNVTKYLKQ EREYVPWKA ALTSLDYLY 
      QMFVRTAHF DKYKKYLLD LLNDFYHEL GFNESENDQ 
      HLTSYNRLE INSRACRLG VRDCIINAV QQFESWRNS 
      PDPDKRNLI SENLREIVY CTAISVGGQ EEWDFAWKR 
      YLNANVENE KETLLMALG CSKEIWILS RFLEWSITE 
      NSGIRKHDS ARVFAAVTS NPIGQQLAY RFLKTHWNR 
      LRTYLGASS MSLSSIVRS CTTKFNSQI EVDDFKMFV 
      DARENEFGV ALRTARQSI EQGEANAKW MMRYSEKIV 
      KWLSVNV
    CDS sequence
    • >TC004952 RA
      atgacccat gctcgcgaa gattttgcc gtaatggaa 
      gcaacagtt acaacgttc ggcaagcag agatttgtc 
      gccgtttcc aaagctcta gcgcttttt attttcatt 
      cttttcgtg ggatgtatc atcgggacg gcgctttta 
      gtctatcac ttatcgtca tgtcagccg atttccaag 
      tttgaagaa accactttg atagtctac gacaagcac 
      tcggacttg gaagttaca acgacgagt gaagaagtc 
      gtaacggaa tcagtgtcc accacaact gaagaaata 
      aagactgaa gaattggac gtgagacta cctagatcg 
      gtcaaaccg cattcttac gccatcaga ctcatccct 
      tttatcgtc gaaggaaac ttcacattc cacggagaa 
      gttacaatt ctcatcaat gtaactgtt actactttt 
      aatgtcacg ttacacgcc gatgatttg gccatagat 
      cgcgtcaac gtctacgac tttaataac gaaatactt 
      tcaattagg caagtcact aatgagact cgtcgtcag 
      ttcttgatt atccacatg aacgaaccg ctgaaatcc 
      ggtcaccag tattatgtc agtattagt tttaaggga 
      gttttgaac gatctactc caaggcttt taccgaagc 
      tcttacagt gttaataac gaactcagg tggattgcc 
      acaacccag tttcaagca acagatgcg cgtaaagct 
      ttcccgtgc ttcgatgaa ccagctttg aaagctcga 
      tttcaaatc agtctcgct agactcaaa aacatgact 
      tcgatctca aacatgccc aaaattgga agtccagaa 
      cctgtcaaa aatctccct gattatttt tgggaccat 
      tacgaagaa agcctccca atgtctacc tacttgatt 
      gctttcgta atctccgac ttcgactgt ctcaaaaac 
      gggagtttc tcggtctgg gcccgcccc agtgcgctt 
      tcacaaacc aaatacagt ctccagata ggtccccaa 
      attttacaa ttctacgaa aacttcttc ggcattaaa 
      taccctctc ccaaaaatc gacatgatc ggtcttccc 
      gacttttcc gcaggggct atggagaat tggggtttg 
      atcacgtac cgggagtcg gttttgctg tacgaagag 
      aaggtttca agtaaagcg agtttgcaa aggatcgct 
      catgtgata gcgcatgaa ttggctcat cagtggttt 
      ggtaatttg gtgactccc gtttggtgg tcggatctg 
      tggttgaac gaagggttt gcgacttat gtggagtgt 
      ttgggggcc aatgcggtc aatccgcat ttgaaagag 
      ttggaccaa tttgtgatt aatgagctc cacggggcc 
      ttggttttg gacgctttg aggacttcg caccaaata 
      tcgatcaaa gtgaataat ccggacgag atcaacgat 
      attttcgac cgtatctcg tactccaaa ggtgcttca 
      attttgcga atgatgcaa catttccta tcaatgcga 
      gtgttccaa aaaggcttg aacaggtat ttgaaaagc 
      cggatgtat agtaatgcg gaacaggac gacttgtgg 
      cacaccctg actttgcaa agtcacgag gataaagtt 
      cttgatcag aacgttaca attaaggag attatggac 
      acttggaca ctacaaact ggttttccg ctagtcaca 
      gcttacagg aactacgaa aatgattca gtcactttc 
      acccaagaa cgttttcta gttaatgac gatgaccaa 
      aggtcaaag tcggttttg tggtggata ccaatcact 
      tacaccaac cccaaaaac gttctccgt tcaaattgg 
      atgcgtaac gaacagatt ttgacaatc catgagtta 
      aagcaaccg aagaaccat tggttactt gtcaatgtt 
      aaccaaacg ggttactac agagtgaat tatgatcca 
      agaaactgg aatttgatt gtgcaacaa ctgttgaag 
      aagaacggc catttggtc tttgacccc aaaaaccgg 
      gcccaactt ctggatgat gctcttcat ctcgcgtcg 
      gttggttac ctcgactat aacattgct ctaaatgtt 
      accaaatat ctgaaacaa gaacgggaa tacgtcccg 
      tggaaagct gccctgact agtttggac tacttgtac 
      caaatgttt gtacgaacg gctcatttc gacaagtat 
      aaaaaatac cttctcgac ttactgaac gatttttac 
      catgagctg ggtttcaac gaaagcgaa aacgaccaa 
      catttgact tcatacaat cgtttagaa attaatagt 
      cgcgcttgc agattaggg gtccgtgat tgtattatc 
      aacgcggtt cagcagttt gaatcctgg aggaactct 
      ccagaccct gacaaacgc aacttgata tcggaaaat 
      ctgcgcgaa attgtatac tgcactgct atcagtgtc 
      ggaggccag gaagagtgg gattttgcg tggaagcgc 
      tacctaaac gccaacgtt gaaaacgag aaagaaact 
      ctcttgatg gctctcggt tgttcaaaa gaaatttgg 
      attttgagc cgttttctc gaatggtct attactgaa 
      aattcgggg attcggaaa cacgactca gctcgtgtt 
      tttgccgct gttacaagt aacccaatt gggcaacaa 
      ctggcttac aggttccta aaaacgcac tggaatcgt 
      ctgaggact tatcttggg gcgtcttcc atgtcattg 
      agtagcata gtcaggagt tgcacgacc aagttcaac 
      agccagatt gaagttgat gattttaaa atgtttgtt 
      gatgcgcgg gaaaatgag tttggtgtt gctttaaga 
      acagcaaga cagtcgata gagcaaggg gaagcgaat 
      gcaaagtgg atgatgaga tattcggag aagattgtt 
      aagtggcta agtgttaat gtctaa
    mRNA sequence
    • >TC004952 RA
      cgaaagacg ctagttcaa agttaggtg tttctgata 
      agagcgata ggggaatca tgtcttagg aggccccag 
      aagcggaat acgaaggca tgacccatg ctcgcgaag 
      attttgccg taatggaag caacagtta caacgttcg 
      gcaagcaga gatttgtcg ccgtttcca aagctctag 
      cgcttttta ttttcattc ttttcgtgg gatgtatca 
      tcgggacgg cgcttttag tctatcact tatcgtcat 
      gtcagccga tttccaagt ttgaagaaa ccactttga 
      tagtctacg acaagcact cggacttgg aagttacaa 
      cgacgagtg aagaagtcg taacggaat cagtgtcca 
      ccacaactg aagaaataa agactgaag aattggacg 
      tgagactac ctagatcgg tcaaaccgc attcttacg 
      ccatcagac tcatccctt ttatcgtcg aaggaaact 
      tcacattcc acggagaag ttacaattc tcatcaatg 
      taactgtta ctactttta atgtcacgt tacacgccg 
      atgatttgg ccatagatc gcgtcaacg tctacgact 
      ttaataacg aaatacttt caattaggc aagtcacta 
      atgagactc gtcgtcagt tcttgatta tccacatga 
      acgaaccgc tgaaatccg gtcaccagt attatgtca 
      gtattagtt ttaagggag ttttgaacg atctactcc 
      aaggctttt accgaagct cttacagtg ttaataacg 
      aactcaggt ggattgcca caacccagt ttcaagcaa 
      cagatgcgc gtaaagctt tcccgtgct tcgatgaac 
      cagctttga aagctcgat ttcaaatca gtctcgcta 
      gactcaaaa acatgactt cgatctcaa acatgccca 
      aaattggaa gtccagaac ctgtcaaaa atctccctg 
      attattttt gggaccatt acgaagaaa gcctcccaa 
      tgtctacct acttgattg ctttcgtaa tctccgact 
      tcgactgtc tcaaaaacg ggagtttct cggtctggg 
      cccgcccca gtgcgcttt cacaaacca aatacagtc 
      tccagatag gtccccaaa ttttacaat tctacgaaa 
      acttcttcg gcattaaat accctctcc caaaaatcg 
      acatgatcg gtcttcccg acttttccg caggggcta 
      tggagaatt ggggtttga tcacgtacc gggagtcgg 
      ttttgctgt acgaagaga aggtttcaa gtaaagcga 
      gtttgcaaa ggatcgctc atgtgatag cgcatgaat 
      tggctcatc agtggtttg gtaatttgg tgactcccg 
      tttggtggt cggatctgt ggttgaacg aagggtttg 
      cgacttatg tggagtgtt tgggggcca atgcggtca 
      atccgcatt tgaaagagt tggaccaat ttgtgatta 
      atgagctcc acggggcct tggttttgg acgctttga 
      ggacttcgc accaaatat cgatcaaag tgaataatc 
      cggacgaga tcaacgata ttttcgacc gtatctcgt 
      actccaaag gtgcttcaa ttttgcgaa tgatgcaac 
      atttcctat caatgcgag tgttccaaa aaggcttga 
      acaggtatt tgaaaagcc ggatgtata gtaatgcgg 
      aacaggacg acttgtggc acaccctga ctttgcaaa 
      gtcacgagg ataaagttc ttgatcaga acgttacaa 
      ttaaggaga ttatggaca cttggacac tacaaactg 
      gttttccgc tagtcacag cttacagga actacgaaa 
      atgattcag tcactttca cccaagaac gttttctag 
      ttaatgacg atgaccaaa ggtcaaagt cggttttgt 
      ggtggatac caatcactt acaccaacc ccaaaaacg 
      ttctccgtt caaattgga tgcgtaacg aacagattt 
      tgacaatcc atgagttaa agcaaccga agaaccatt 
      ggttacttg tcaatgtta accaaacgg gttactaca 
      gagtgaatt atgatccaa gaaactgga atttgattg 
      tgcaacaac tgttgaaga agaacggcc atttggtct 
      ttgacccca aaaaccggg cccaacttc tggatgatg 
      ctcttcatc tcgcgtcgg ttggttacc tcgactata 
      acattgctc taaatgtta ccaaatatc tgaaacaag 
      aacgggaat acgtcccgt ggaaagctg ccctgacta 
      gtttggact acttgtacc aaatgtttg tacgaacgg 
      ctcatttcg acaagtata aaaaatacc ttctcgact 
      tactgaacg atttttacc atgagctgg gtttcaacg 
      aaagcgaaa acgaccaac atttgactt catacaatc 
      gtttagaaa ttaatagtc gcgcttgca gattagggg 
      tccgtgatt gtattatca acgcggttc agcagtttg 
      aatcctgga ggaactctc cagaccctg acaaacgca 
      acttgatat cggaaaatc tgcgcgaaa ttgtatact 
      gcactgcta tcagtgtcg gaggccagg aagagtggg 
      attttgcgt ggaagcgct acctaaacg ccaacgttg 
      aaaacgaga aagaaactc tcttgatgg ctctcggtt 
      gttcaaaag aaatttgga ttttgagcc gttttctcg 
      aatggtcta ttactgaaa attcgggga ttcggaaac 
      acgactcag ctcgtgttt ttgccgctg ttacaagta 
      acccaattg ggcaacaac tggcttaca ggttcctaa 
      aaacgcact ggaatcgtc tgaggactt atcttgggg 
      cgtcttcca tgtcattga gtagcatag tcaggagtt 
      gcacgacca agttcaaca gccagattg aagttgatg 
      attttaaaa tgtttgttg atgcgcggg aaaatgagt 
      ttggtgttg ctttaagaa cagcaagac agtcgatag 
      agcaagggg aagcgaatg caaagtgga tgatgagat 
      attcggaga agattgtta agtggctaa gtgttaatg 
      tctaagtga ctttaaatg tattactga atcaaattg 
      atttgaatt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY