Status
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Gene information
General information
TC030986
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004924,TC004925
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4710800..4729199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030986
    iBeetle screen iB_03873

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030986 RA
      MGQQPRPQL NMNPSAPAR SPGYHQKAM EEIQNSLRP 
      FAKSGGEAV GSSAASTIS NFSATSGVS SLSSTSGTN 
      GTDKDVLQL RHTLSQLVN MGYSEEASL RALQLSNSR 
      LDGALDYLM KQQIDSGNK CGYTKLIRK PSIERELVL 
      PRGSPALDS GAGSSRSDS PRLTEIHST VSRYSPNFS 
      EPPPPPPPR NTPPPPPPP HVPSSYPAL NNVQQLLKR 
      MSPAPIVPA RAPPAVPSY NSPALQQRG TSPVSSVSS 
      SSGRQPMVV QNGPQVQQQ LSQQIQALS LYQSSSAST 
      AEPPPPYPL IPPSPSNSA PPPPSYSAS MQNRHSPTQ 
      DFRKSPSSG IYSGSTSAG SPSPIPVST TTPTAMARP 
      TPLLAWGAR QTKTQPPII MQSVKSTQV QKPVLQTAV 
      APTVPQIPT SPTSTTPLV TCAPPPSYT ASIQQKGYT 
      TSPKPAAPL PTTPANNTT PVVVPTTEP PSYASTMQA 
      KAKAQRGMG LSPHPPPPY SDENHAQVN TVECAMSPR 
      ASPNVHPPL QRKYSPVVS SECAASRSD SPQSTSSGD 
      SRPSYPDNG APPLPPTAS SSVKNNHNA INGNGESKN 
      NDIPPQLPP YTKIKHQSP IPERKKISK EKEDERRDG 
      KVKNYSPQA FKFFMEQHI ENVIKSYKQ RMSRRIQLE 
      NEMTKIGLS SEAQKQMRK MLSQKESNY IRLKRAKMD 
      KSMFVKIKP IGVGAFGEV TLVRKIDTK HLYAMKTLR 
      KADVLKRNQ VAHVKAERD ILAEADNEW VVKLYYSFQ 
      DSDILYFVM DYIPGGDLM SLLIKLGVF EEHLARFYI 
      AELTCAVES VHKMGFIHR DIKPDNILI DKEGHIKLT 
      DFGLCTGFR WTHNSKYYQ PNGDHSRQD SMDPIEDWG 
      EECKCMKPL ERRRRREHR CLAHSLVGT PNYIAPEVL 
      QRIGYTHVC DWWSVGVIL YEMLVGQPP FLANTPAET 
      QHKVINWET TLQIPKQAN LSKESTDLI LKLCCSADK 
      RLGKNASEV KSHPFFKDI DFDRGLRRQ PAPHIPRIE 
      YPTDTSNFD PIDPDKLRN SASIDSSTS DELTDSSKP 
      FHGFFEFTF RRFFDDGVV LIGDSGVGK SNLLSRFTR 
      NEFNLESKS TIGVEFATR SMQVDGKTI KAQIWDTAG 
      QERYRAITA AYYRGAVGA LLVYDIAKH LTYENVERW 
      LRELRDHAD QNIVIMLVG NKSDLRHLR AVLTEEAKA 
      FAERNGLSF IETSALDST NVDLAFQNI LTEIYRIVS 
      QKQIRDPPE GDVIRPHNV EPIDVKPTM NTDGPYTEF 
      CIM
    CDS sequence
    • >TC030986 RA
      atgggccaa cagcctcgg ccccaactg aacatgaat 
      cccagcgcg ccagcccgc tcgccgggc taccaccaa 
      aaggccatg gaggagatc cagaactcg ctgcgcccg 
      ttcgcaaag agcggcggc gaggccgtg ggctccagt 
      gcggccagc accatttcg aatttctcc gccacaagc 
      ggagtgagc tcgctgagc tcgacgtcc ggcactaac 
      ggcaccgac aaggacgtc ctccagctc agacacacg 
      ctctcgcag ctcgtcaac atgggctat tcggaggag 
      gcctcgttg cgagcgctc caactctcc aactcacgt 
      ctggacggc gccctcgac tatctcatg aaacaacaa 
      atcgactcc ggaaacaaa tgcggttac accaaactc 
      atacgtaag cctagcatc gaacgagaa ttggttctg 
      ccgagagga agtccggct ttggatagc ggggccgga 
      agttcccga tctgatagt ccgagattg accgaaata 
      cactcaaca gtcagccga tactctccg aatttcagc 
      gaaccgcca cccccacca ccacctcgg aacacgccg 
      ccaccgccg ccgccacct cacgtccca tcctcatac 
      cccgcatta aacaacgtt caacaatta ctaaaacga 
      atgtcaccc gcgcccatt gtaccagca agagcgcca 
      cctgccgta ccttcatat aactcacca gcgttgcaa 
      cagcgagga actagtcct gttagtagt gtgtcctct 
      tcttccggt cggcaacca atggtagtc caaaacggc 
      ccccaagtc caacaacaa ctatctcaa caaatccaa 
      gctctgagc ttgtatcag agcagtagc gcgtcaact 
      gccgaacct ccgccccct tatcctcta atcccgccc 
      agtccttcc aactccgcc ccgccccct ccgtcatac 
      tccgcctcg atgcagaac cggcacagc cccactcaa 
      gacttccgt aaaagcccc tcgtctggc atttattcc 
      ggctcaact tcagccggt tctcctagt cctatccca 
      gtctcaaca accacgccc acagccatg gcccgcccc 
      acccctctc ctggcatgg ggcgcccgc caaaccaaa 
      actcaaccc cccatcatc atgcaatcc gttaaaagt 
      actcaagtc cagaaaccc gtactgcaa actgccgtc 
      gcaccgact gttcctcaa atcccgacg tctccaacg 
      agtactact cccttagtc acctgcgcg ccccctccg 
      tcatacacc gcttccatc caacagaag gggtacacc 
      accagtcct aaaccggcg gctcctttg cccaccaca 
      cccgccaat aacaccact cctgtggtg gtaccaacg 
      acagaaccg ccgagttac gccagtacc atgcaagcg 
      aaagctaaa gctcaacgg gggatgggg ttatcgccc 
      catccaccg ccgccttat tcggacgaa aatcatgct 
      caagtcaat acagtcgaa tgtgcgatg tcgccgagg 
      gcgtcccca aatgttcat cctcctctt cagaggaag 
      tactcgccg gttgtgtct tcggagtgc gcagcatcg 
      aggtccgat agtccgcag tcgacgtcg tcgggggat 
      agcaggcct tcgtatccc gataatggg gccccacca 
      ctgccacca acagcatcc tcatctgtc aaaaacaac 
      cacaacgcg ataaacgga aacggcgag agcaagaat 
      aacgacatc ccgccgcaa ttacctcct tacacaaaa 
      ataaaacat cagtcaccg ataccggaa cggaaaaag 
      attagtaaa gaaaaagaa gacgaaagg cgggacggc 
      aaagtgaag aattactcg cctcaagca ttcaaattc 
      ttcatggaa cagcacatc gagaacgtc atcaaatcg 
      tacaaacag cgaatgtcg agaagaatc caactcgaa 
      aacgaaatg acaaaaatc ggtttgagt tccgaggca 
      caaaaacaa atgcgtaaa atgctctcg caaaaagaa 
      tcgaattac attagactc aaacgagca aaaatggac 
      aaaagtatg tttgttaag atcaaaccg attggagtt 
      ggggcgttc ggagaggtg actctagtg aggaaaatc 
      gacacgaaa catttgtac gcgatgaaa accttgcga 
      aaagctgac gttctgaag aggaatcaa gtggcgcac 
      gtgaaagcg gagcgagac atccttgca gaggctgat 
      aacgaatgg gttgttaaa ttgtattat tcgtttcaa 
      gatagtgat attctttat tttgttatg gactatatc 
      cccggcggg gatcttatg tcgttgttg atcaagttg 
      ggggttttc gaagagcat ttggcaaga ttttatatt 
      gcggaactg acttgcgcc gtcgaaagt gtgcataaa 
      atgggtttc attcatcgc gatatcaag ccggacaat 
      attttgatc gataaagaa ggtcacatt aaactcacc 
      gatttcggt ttgtgtaca ggatttagg tggacacat 
      aactcgaaa tattaccaa cctaacggt gaccacagt 
      agacaggat tctatggac ccgattgaa gattgggga 
      gaggagtgc aagtgtatg aaaccgtta gaaaggaga 
      aggcggagg gaacatcga tgtttggca cattcgtta 
      gttggcact ccgaattat atcgcacct gaagtgttg 
      caacgtata ggatatacg cacgtctgt gattggtgg 
      agtgtcggt gttatcctg tatgagatg ttggtgggg 
      caacctccg ttcctcgcg aatacgcct gcagaaaca 
      caacacaag gtgataaac tgggagacg acgttgcaa 
      ataccaaag caagcgaat ctttccaag gaaagtact 
      gacttgata ttaaaattg tgttgtagt gcggacaaa 
      cgcttaggt aaaaatgct agtgaagtg aagtcgcat 
      ccgttcttc aaagacatc gatttcgac cgaggtttg 
      cgtcgccag ccggccccg cacatacct cgaatcgaa 
      taccccaca gatacgtca aatttcgat ccgatcgat 
      cccgacaag ttacgaaat tcggcttcg atcgattcg 
      agcacttcc gacgaactg accgatagt tctaagccg 
      tttcacggc tttttcgaa ttcacattc aggcgtttt 
      ttcgatgac ggagttgtc ctcattgga gactccggg 
      gtaggaaaa agcaatctg ttgtctaga tttactagg 
      aatgaattt aatttggaa tcaaaatcg acaatagga 
      gtggaattc gcgactcgt agcatgcag gtcgatggt 
      aaaacaata aaggcacaa atctgggat acggcagga 
      caggaaaga taccgtgcc atcacagcg gcgtattac 
      agaggggcc gtcggtgct ctgttagtc tacgacatt 
      gctaagcat ttgacttac gaaaatgtg gagcgttgg 
      ttgcgtgag ctccgggac cacgcggac caaaacatc 
      gtgatcatg ttggttggc aataaatcg gatttaagg 
      catctacgc gccgtccta accgaggag gcgaaagct 
      ttcgccgaa agaaacggc ctctcattc atagaaacg 
      tcagctctt gattcgacc aacgtcgat ttggctttc 
      cagaacata ctcacagaa atctaccga atcgtatca 
      cagaaacaa atcagagat cctcccgaa ggcgacgtt 
      attcgacca cataacgtg gaaccaata gacgtaaaa 
      ccaacgatg aacacggat ggtccctac acagaattc 
      tgcatcatg tag
    mRNA sequence
    • >TC030986 RA
      tgacacttc aaaatctgt cagttcttg cagcagatt 
      ccatttgtt ttagaagaa taaaagtcg tgaagtcgc 
      gaacgatcg aatccactg caagaatcg tcaggagtg 
      cgtcaaaac tcgtcttcc aatgtaatg caaaatgta 
      tgaatgggc gtaattgta gtgcatact catgtgcgg 
      ggagccatg tgaatcgct agtgagtgg cagggccca 
      gcatgggcc aacagcctc ggccccaac tgaacatga 
      atcccagcg cgccagccc gctcgccgg gctaccacc 
      aaaaggcca tggaggaga tccagaact cgctgcgcc 
      cgttcgcaa agagcggcg gcgaggccg tgggctcca 
      gtgcggcca gcaccattt cgaatttct ccgccacaa 
      gcggagtga gctcgctga gctcgacgt ccggcacta 
      acggcaccg acaaggacg tcctccagc tcagacaca 
      cgctctcgc agctcgtca acatgggct attcggagg 
      aggcctcgt tgcgagcgc tccaactct ccaactcac 
      gtctggacg gcgccctcg actatctca tgaaacaac 
      aaatcgact ccggaaaca aatgcggtt acaccaaac 
      tcatacgta agcctagca tcgaacgag aattggttc 
      tgccgagag gaagtccgg ctttggata gcggggccg 
      gaagttccc gatctgata gtccgagat tgaccgaaa 
      tacactcaa cagtcagcc gatactctc cgaatttca 
      gcgaaccgc cacccccac caccacctc ggaacacgc 
      cgccaccgc cgccgccac ctcacgtcc catcctcat 
      accccgcat taaacaacg ttcaacaat tactaaaac 
      gaatgtcac ccgcgccca ttgtaccag caagagcgc 
      cacctgccg taccttcat ataactcac cagcgttgc 
      aacagcgag gaactagtc ctgttagta gtgtgtcct 
      cttcttccg gtcggcaac caatggtag tccaaaacg 
      gcccccaag tccaacaac aactatctc aacaaatcc 
      aagctctga gcttgtatc agagcagta gcgcgtcaa 
      ctgccgaac ctccgcccc cttatcctc taatcccgc 
      ccagtcctt ccaactccg ccccgcccc ctccgtcat 
      actccgcct cgatgcaga accggcaca gccccactc 
      aagacttcc gtaaaagcc cctcgtctg gcatttatt 
      ccggctcaa cttcagccg gttctccta gtcctatcc 
      cagtctcaa caaccacgc ccacagcca tggcccgcc 
      ccacccctc tcctggcat ggggcgccc gccaaacca 
      aaactcaac cccccatca tcatgcaat ccgttaaaa 
      gtactcaag tccagaaac ccgtactgc aaactgccg 
      tcgcaccga ctgttcctc aaatcccga cgtctccaa 
      cgagtacta ctcccttag tcacctgcg cgccccctc 
      cgtcataca ccgcttcca tccaacaga aggggtaca 
      ccaccagtc ctaaaccgg cggctcctt tgcccacca 
      cacccgcca ataacacca ctcctgtgg tggtaccaa 
      cgacagaac cgccgagtt acgccagta ccatgcaag 
      cgaaagcta aagctcaac gggggatgg ggttatcgc 
      cccatccac cgccgcctt attcggacg aaaatcatg 
      ctcaagtca atacagtcg aatgtgcga tgtcgccga 
      gggcgtccc caaatgttc atcctcctc ttcagagga 
      agtactcgc cggttgtgt cttcggagt gcgcagcat 
      cgaggtccg atagtccgc agtcgacgt cgtcggggg 
      atagcaggc cttcgtatc ccgataatg gggccccac 
      cactgccac caacagcat cctcatctg tcaaaaaca 
      accacaacg cgataaacg gaaacggcg agagcaaga 
      ataacgaca tcccgccgc aattacctc cttacacaa 
      aaataaaac atcagtcac cgataccgg aacggaaaa 
      agattagta aagaaaaag aagacgaaa ggcgggacg 
      gcaaagtga agaattact cgcctcaag cattcaaat 
      tcttcatgg aacagcaca tcgagaacg tcatcaaat 
      cgtacaaac agcgaatgt cgagaagaa tccaactcg 
      aaaacgaaa tgacaaaaa tcggtttga gttccgagg 
      cacaaaaac aaatgcgta aaatgctct cgcaaaaag 
      aatcgaatt acattagac tcaaacgag caaaaatgg 
      acaaaagta tgtttgtta agatcaaac cgattggag 
      ttggggcgt tcggagagg tgactctag tgaggaaaa 
      tcgacacga aacatttgt acgcgatga aaaccttgc 
      gaaaagctg acgttctga agaggaatc aagtggcgc 
      acgtgaaag cggagcgag acatccttg cagaggctg 
      ataacgaat gggttgtta aattgtatt attcgtttc 
      aagatagtg atattcttt attttgtta tggactata 
      tccccggcg gggatctta tgtcgttgt tgatcaagt 
      tgggggttt tcgaagagc atttggcaa gattttata 
      ttgcggaac tgacttgcg ccgtcgaaa gtgtgcata 
      aaatgggtt tcattcatc gcgatatca agccggaca 
      atattttga tcgataaag aaggtcaca ttaaactca 
      ccgatttcg gtttgtgta caggattta ggtggacac 
      ataactcga aatattacc aacctaacg gtgaccaca 
      gtagacagg attctatgg acccgattg aagattggg 
      gagaggagt gcaagtgta tgaaaccgt tagaaagga 
      gaaggcgga gggaacatc gatgtttgg cacattcgt 
      tagttggca ctccgaatt atatcgcac ctgaagtgt 
      tgcaacgta taggatata cgcacgtct gtgattggt 
      ggagtgtcg gtgttatcc tgtatgaga tgttggtgg 
      ggcaacctc cgttcctcg cgaatacgc ctgcagaaa 
      cacaacaca aggtgataa actgggaga cgacgttgc 
      aaataccaa agcaagcga atctttcca aggaaagta 
      ctgacttga tattaaaat tgtgttgta gtgcggaca 
      aacgcttag gtaaaaatg ctagtgaag tgaagtcgc 
      atccgttct tcaaagaca tcgatttcg accgaggtt 
      tgcgtcgcc agccggccc cgcacatac ctcgaatcg 
      aatacccca cagatacgt caaatttcg atccgatcg 
      atcccgaca agttacgaa attcggctt cgatcgatt 
      cgagcactt ccgacgaac tgaccgata gttctaagc 
      cgtttcacg gctttttcg aattcacat tcaggcgtt 
      ttttcgatg acggagttg tcctcattg gagactccg 
      gggtaggaa aaagcaatc tgttgtcta gatttacta 
      ggaatgaat ttaatttgg aatcaaaat cgacaatag 
      gagtggaat tcgcgactc gtagcatgc aggtcgatg 
      gtaaaacaa taaaggcac aaatctggg atacggcag 
      gacaggaaa gataccgtg ccatcacag cggcgtatt 
      acagagggg ccgtcggtg ctctgttag tctacgaca 
      ttgctaagc atttgactt acgaaaatg tggagcgtt 
      ggttgcgtg agctccggg accacgcgg accaaaaca 
      tcgtgatca tgttggttg gcaataaat cggatttaa 
      ggcatctac gcgccgtcc taaccgagg aggcgaaag 
      ctttcgccg aaagaaacg gcctctcat tcatagaaa 
      cgtcagctc ttgattcga ccaacgtcg atttggctt 
      tccagaaca tactcacag aaatctacc gaatcgtat 
      cacagaaac aaatcagag atcctcccg aaggcgacg 
      ttattcgac cacataacg tggaaccaa tagacgtaa 
      aaccaacga tgaacacgg atggtccct acacagaat 
      tctgcatca tgtagtgag cctctgtac cacccaccc 
      atggctatt tgtgttttt gttataatt tacataaat 
      ctgaatgtc ctaatgcta acatattgt gacttagcg 
      tggagactc cacatctgt ttgctgcgc acttagagc 
      tgttacagt tttgtatac tattataat ttcagtaaa 
      ttttttcta tataaaata taactgtag gtatttaag 
      taatgaaaa gtttattta tcaatccta tgctttaag 
      gcatgtgat catatttaa cgatagttt tatcaattc 
      aggtacaga cctaagttt gaatatctg tgtctctga 
      tagatatta aaaacaata gtccttggt tttattgta 
      aatgtgctc tgttattta taattgaaa atttactac 
      aatataata accgttatt tgctggttt tgttgattt 
      ggtaaagct agacgtgga ctaccgttc cagaatcgg 
      ggcaaacac ccgacagta ccattccag tgtattatt 
      ttttcgcaa gttttgtga aagactgtt gcagtttgc 
      tcaggttta ggaaggata gttgaagtt accaaaatt 
      cgcgttaca aaatttgtt tttgttact gtctttagc 
      tatattaaa gttaatagg tcagattgt acacctgta 
      acaaaataa cggtctttg tatgtaata taagcttat 
      tcgaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY