| General information | |||
| TC030986
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 4710800..4729199 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030986 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03873 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC030986 RA
MGQQPRPQL NMNPSAPAR SPGYHQKAM EEIQNSLRP FAKSGGEAV GSSAASTIS NFSATSGVS SLSSTSGTN GTDKDVLQL RHTLSQLVN MGYSEEASL RALQLSNSR LDGALDYLM KQQIDSGNK CGYTKLIRK PSIERELVL PRGSPALDS GAGSSRSDS PRLTEIHST VSRYSPNFS EPPPPPPPR NTPPPPPPP HVPSSYPAL NNVQQLLKR MSPAPIVPA RAPPAVPSY NSPALQQRG TSPVSSVSS SSGRQPMVV QNGPQVQQQ LSQQIQALS LYQSSSAST AEPPPPYPL IPPSPSNSA PPPPSYSAS MQNRHSPTQ DFRKSPSSG IYSGSTSAG SPSPIPVST TTPTAMARP TPLLAWGAR QTKTQPPII MQSVKSTQV QKPVLQTAV APTVPQIPT SPTSTTPLV TCAPPPSYT ASIQQKGYT TSPKPAAPL PTTPANNTT PVVVPTTEP PSYASTMQA KAKAQRGMG LSPHPPPPY SDENHAQVN TVECAMSPR ASPNVHPPL QRKYSPVVS SECAASRSD SPQSTSSGD SRPSYPDNG APPLPPTAS SSVKNNHNA INGNGESKN NDIPPQLPP YTKIKHQSP IPERKKISK EKEDERRDG KVKNYSPQA FKFFMEQHI ENVIKSYKQ RMSRRIQLE NEMTKIGLS SEAQKQMRK MLSQKESNY IRLKRAKMD KSMFVKIKP IGVGAFGEV TLVRKIDTK HLYAMKTLR KADVLKRNQ VAHVKAERD ILAEADNEW VVKLYYSFQ DSDILYFVM DYIPGGDLM SLLIKLGVF EEHLARFYI AELTCAVES VHKMGFIHR DIKPDNILI DKEGHIKLT DFGLCTGFR WTHNSKYYQ PNGDHSRQD SMDPIEDWG EECKCMKPL ERRRRREHR CLAHSLVGT PNYIAPEVL QRIGYTHVC DWWSVGVIL YEMLVGQPP FLANTPAET QHKVINWET TLQIPKQAN LSKESTDLI LKLCCSADK RLGKNASEV KSHPFFKDI DFDRGLRRQ PAPHIPRIE YPTDTSNFD PIDPDKLRN SASIDSSTS DELTDSSKP FHGFFEFTF RRFFDDGVV LIGDSGVGK SNLLSRFTR NEFNLESKS TIGVEFATR SMQVDGKTI KAQIWDTAG QERYRAITA AYYRGAVGA LLVYDIAKH LTYENVERW LRELRDHAD QNIVIMLVG NKSDLRHLR AVLTEEAKA FAERNGLSF IETSALDST NVDLAFQNI LTEIYRIVS QKQIRDPPE GDVIRPHNV EPIDVKPTM NTDGPYTEF CIM
- >TC030986 RA
atgggccaa cagcctcgg ccccaactg aacatgaat cccagcgcg ccagcccgc tcgccgggc taccaccaa aaggccatg gaggagatc cagaactcg ctgcgcccg ttcgcaaag agcggcggc gaggccgtg ggctccagt gcggccagc accatttcg aatttctcc gccacaagc ggagtgagc tcgctgagc tcgacgtcc ggcactaac ggcaccgac aaggacgtc ctccagctc agacacacg ctctcgcag ctcgtcaac atgggctat tcggaggag gcctcgttg cgagcgctc caactctcc aactcacgt ctggacggc gccctcgac tatctcatg aaacaacaa atcgactcc ggaaacaaa tgcggttac accaaactc atacgtaag cctagcatc gaacgagaa ttggttctg ccgagagga agtccggct ttggatagc ggggccgga agttcccga tctgatagt ccgagattg accgaaata cactcaaca gtcagccga tactctccg aatttcagc gaaccgcca cccccacca ccacctcgg aacacgccg ccaccgccg ccgccacct cacgtccca tcctcatac cccgcatta aacaacgtt caacaatta ctaaaacga atgtcaccc gcgcccatt gtaccagca agagcgcca cctgccgta ccttcatat aactcacca gcgttgcaa cagcgagga actagtcct gttagtagt gtgtcctct tcttccggt cggcaacca atggtagtc caaaacggc ccccaagtc caacaacaa ctatctcaa caaatccaa gctctgagc ttgtatcag agcagtagc gcgtcaact gccgaacct ccgccccct tatcctcta atcccgccc agtccttcc aactccgcc ccgccccct ccgtcatac tccgcctcg atgcagaac cggcacagc cccactcaa gacttccgt aaaagcccc tcgtctggc atttattcc ggctcaact tcagccggt tctcctagt cctatccca gtctcaaca accacgccc acagccatg gcccgcccc acccctctc ctggcatgg ggcgcccgc caaaccaaa actcaaccc cccatcatc atgcaatcc gttaaaagt actcaagtc cagaaaccc gtactgcaa actgccgtc gcaccgact gttcctcaa atcccgacg tctccaacg agtactact cccttagtc acctgcgcg ccccctccg tcatacacc gcttccatc caacagaag gggtacacc accagtcct aaaccggcg gctcctttg cccaccaca cccgccaat aacaccact cctgtggtg gtaccaacg acagaaccg ccgagttac gccagtacc atgcaagcg aaagctaaa gctcaacgg gggatgggg ttatcgccc catccaccg ccgccttat tcggacgaa aatcatgct caagtcaat acagtcgaa tgtgcgatg tcgccgagg gcgtcccca aatgttcat cctcctctt cagaggaag tactcgccg gttgtgtct tcggagtgc gcagcatcg aggtccgat agtccgcag tcgacgtcg tcgggggat agcaggcct tcgtatccc gataatggg gccccacca ctgccacca acagcatcc tcatctgtc aaaaacaac cacaacgcg ataaacgga aacggcgag agcaagaat aacgacatc ccgccgcaa ttacctcct tacacaaaa ataaaacat cagtcaccg ataccggaa cggaaaaag attagtaaa gaaaaagaa gacgaaagg cgggacggc aaagtgaag aattactcg cctcaagca ttcaaattc ttcatggaa cagcacatc gagaacgtc atcaaatcg tacaaacag cgaatgtcg agaagaatc caactcgaa aacgaaatg acaaaaatc ggtttgagt tccgaggca caaaaacaa atgcgtaaa atgctctcg caaaaagaa tcgaattac attagactc aaacgagca aaaatggac aaaagtatg tttgttaag atcaaaccg attggagtt ggggcgttc ggagaggtg actctagtg aggaaaatc gacacgaaa catttgtac gcgatgaaa accttgcga aaagctgac gttctgaag aggaatcaa gtggcgcac gtgaaagcg gagcgagac atccttgca gaggctgat aacgaatgg gttgttaaa ttgtattat tcgtttcaa gatagtgat attctttat tttgttatg gactatatc cccggcggg gatcttatg tcgttgttg atcaagttg ggggttttc gaagagcat ttggcaaga ttttatatt gcggaactg acttgcgcc gtcgaaagt gtgcataaa atgggtttc attcatcgc gatatcaag ccggacaat attttgatc gataaagaa ggtcacatt aaactcacc gatttcggt ttgtgtaca ggatttagg tggacacat aactcgaaa tattaccaa cctaacggt gaccacagt agacaggat tctatggac ccgattgaa gattgggga gaggagtgc aagtgtatg aaaccgtta gaaaggaga aggcggagg gaacatcga tgtttggca cattcgtta gttggcact ccgaattat atcgcacct gaagtgttg caacgtata ggatatacg cacgtctgt gattggtgg agtgtcggt gttatcctg tatgagatg ttggtgggg caacctccg ttcctcgcg aatacgcct gcagaaaca caacacaag gtgataaac tgggagacg acgttgcaa ataccaaag caagcgaat ctttccaag gaaagtact gacttgata ttaaaattg tgttgtagt gcggacaaa cgcttaggt aaaaatgct agtgaagtg aagtcgcat ccgttcttc aaagacatc gatttcgac cgaggtttg cgtcgccag ccggccccg cacatacct cgaatcgaa taccccaca gatacgtca aatttcgat ccgatcgat cccgacaag ttacgaaat tcggcttcg atcgattcg agcacttcc gacgaactg accgatagt tctaagccg tttcacggc tttttcgaa ttcacattc aggcgtttt ttcgatgac ggagttgtc ctcattgga gactccggg gtaggaaaa agcaatctg ttgtctaga tttactagg aatgaattt aatttggaa tcaaaatcg acaatagga gtggaattc gcgactcgt agcatgcag gtcgatggt aaaacaata aaggcacaa atctgggat acggcagga caggaaaga taccgtgcc atcacagcg gcgtattac agaggggcc gtcggtgct ctgttagtc tacgacatt gctaagcat ttgacttac gaaaatgtg gagcgttgg ttgcgtgag ctccgggac cacgcggac caaaacatc gtgatcatg ttggttggc aataaatcg gatttaagg catctacgc gccgtccta accgaggag gcgaaagct ttcgccgaa agaaacggc ctctcattc atagaaacg tcagctctt gattcgacc aacgtcgat ttggctttc cagaacata ctcacagaa atctaccga atcgtatca cagaaacaa atcagagat cctcccgaa ggcgacgtt attcgacca cataacgtg gaaccaata gacgtaaaa ccaacgatg aacacggat ggtccctac acagaattc tgcatcatg tag
- >TC030986 RA
tgacacttc aaaatctgt cagttcttg cagcagatt ccatttgtt ttagaagaa taaaagtcg tgaagtcgc gaacgatcg aatccactg caagaatcg tcaggagtg cgtcaaaac tcgtcttcc aatgtaatg caaaatgta tgaatgggc gtaattgta gtgcatact catgtgcgg ggagccatg tgaatcgct agtgagtgg cagggccca gcatgggcc aacagcctc ggccccaac tgaacatga atcccagcg cgccagccc gctcgccgg gctaccacc aaaaggcca tggaggaga tccagaact cgctgcgcc cgttcgcaa agagcggcg gcgaggccg tgggctcca gtgcggcca gcaccattt cgaatttct ccgccacaa gcggagtga gctcgctga gctcgacgt ccggcacta acggcaccg acaaggacg tcctccagc tcagacaca cgctctcgc agctcgtca acatgggct attcggagg aggcctcgt tgcgagcgc tccaactct ccaactcac gtctggacg gcgccctcg actatctca tgaaacaac aaatcgact ccggaaaca aatgcggtt acaccaaac tcatacgta agcctagca tcgaacgag aattggttc tgccgagag gaagtccgg ctttggata gcggggccg gaagttccc gatctgata gtccgagat tgaccgaaa tacactcaa cagtcagcc gatactctc cgaatttca gcgaaccgc cacccccac caccacctc ggaacacgc cgccaccgc cgccgccac ctcacgtcc catcctcat accccgcat taaacaacg ttcaacaat tactaaaac gaatgtcac ccgcgccca ttgtaccag caagagcgc cacctgccg taccttcat ataactcac cagcgttgc aacagcgag gaactagtc ctgttagta gtgtgtcct cttcttccg gtcggcaac caatggtag tccaaaacg gcccccaag tccaacaac aactatctc aacaaatcc aagctctga gcttgtatc agagcagta gcgcgtcaa ctgccgaac ctccgcccc cttatcctc taatcccgc ccagtcctt ccaactccg ccccgcccc ctccgtcat actccgcct cgatgcaga accggcaca gccccactc aagacttcc gtaaaagcc cctcgtctg gcatttatt ccggctcaa cttcagccg gttctccta gtcctatcc cagtctcaa caaccacgc ccacagcca tggcccgcc ccacccctc tcctggcat ggggcgccc gccaaacca aaactcaac cccccatca tcatgcaat ccgttaaaa gtactcaag tccagaaac ccgtactgc aaactgccg tcgcaccga ctgttcctc aaatcccga cgtctccaa cgagtacta ctcccttag tcacctgcg cgccccctc cgtcataca ccgcttcca tccaacaga aggggtaca ccaccagtc ctaaaccgg cggctcctt tgcccacca cacccgcca ataacacca ctcctgtgg tggtaccaa cgacagaac cgccgagtt acgccagta ccatgcaag cgaaagcta aagctcaac gggggatgg ggttatcgc cccatccac cgccgcctt attcggacg aaaatcatg ctcaagtca atacagtcg aatgtgcga tgtcgccga gggcgtccc caaatgttc atcctcctc ttcagagga agtactcgc cggttgtgt cttcggagt gcgcagcat cgaggtccg atagtccgc agtcgacgt cgtcggggg atagcaggc cttcgtatc ccgataatg gggccccac cactgccac caacagcat cctcatctg tcaaaaaca accacaacg cgataaacg gaaacggcg agagcaaga ataacgaca tcccgccgc aattacctc cttacacaa aaataaaac atcagtcac cgataccgg aacggaaaa agattagta aagaaaaag aagacgaaa ggcgggacg gcaaagtga agaattact cgcctcaag cattcaaat tcttcatgg aacagcaca tcgagaacg tcatcaaat cgtacaaac agcgaatgt cgagaagaa tccaactcg aaaacgaaa tgacaaaaa tcggtttga gttccgagg cacaaaaac aaatgcgta aaatgctct cgcaaaaag aatcgaatt acattagac tcaaacgag caaaaatgg acaaaagta tgtttgtta agatcaaac cgattggag ttggggcgt tcggagagg tgactctag tgaggaaaa tcgacacga aacatttgt acgcgatga aaaccttgc gaaaagctg acgttctga agaggaatc aagtggcgc acgtgaaag cggagcgag acatccttg cagaggctg ataacgaat gggttgtta aattgtatt attcgtttc aagatagtg atattcttt attttgtta tggactata tccccggcg gggatctta tgtcgttgt tgatcaagt tgggggttt tcgaagagc atttggcaa gattttata ttgcggaac tgacttgcg ccgtcgaaa gtgtgcata aaatgggtt tcattcatc gcgatatca agccggaca atattttga tcgataaag aaggtcaca ttaaactca ccgatttcg gtttgtgta caggattta ggtggacac ataactcga aatattacc aacctaacg gtgaccaca gtagacagg attctatgg acccgattg aagattggg gagaggagt gcaagtgta tgaaaccgt tagaaagga gaaggcgga gggaacatc gatgtttgg cacattcgt tagttggca ctccgaatt atatcgcac ctgaagtgt tgcaacgta taggatata cgcacgtct gtgattggt ggagtgtcg gtgttatcc tgtatgaga tgttggtgg ggcaacctc cgttcctcg cgaatacgc ctgcagaaa cacaacaca aggtgataa actgggaga cgacgttgc aaataccaa agcaagcga atctttcca aggaaagta ctgacttga tattaaaat tgtgttgta gtgcggaca aacgcttag gtaaaaatg ctagtgaag tgaagtcgc atccgttct tcaaagaca tcgatttcg accgaggtt tgcgtcgcc agccggccc cgcacatac ctcgaatcg aatacccca cagatacgt caaatttcg atccgatcg atcccgaca agttacgaa attcggctt cgatcgatt cgagcactt ccgacgaac tgaccgata gttctaagc cgtttcacg gctttttcg aattcacat tcaggcgtt ttttcgatg acggagttg tcctcattg gagactccg gggtaggaa aaagcaatc tgttgtcta gatttacta ggaatgaat ttaatttgg aatcaaaat cgacaatag gagtggaat tcgcgactc gtagcatgc aggtcgatg gtaaaacaa taaaggcac aaatctggg atacggcag gacaggaaa gataccgtg ccatcacag cggcgtatt acagagggg ccgtcggtg ctctgttag tctacgaca ttgctaagc atttgactt acgaaaatg tggagcgtt ggttgcgtg agctccggg accacgcgg accaaaaca tcgtgatca tgttggttg gcaataaat cggatttaa ggcatctac gcgccgtcc taaccgagg aggcgaaag ctttcgccg aaagaaacg gcctctcat tcatagaaa cgtcagctc ttgattcga ccaacgtcg atttggctt tccagaaca tactcacag aaatctacc gaatcgtat cacagaaac aaatcagag atcctcccg aaggcgacg ttattcgac cacataacg tggaaccaa tagacgtaa aaccaacga tgaacacgg atggtccct acacagaat tctgcatca tgtagtgag cctctgtac cacccaccc atggctatt tgtgttttt gttataatt tacataaat ctgaatgtc ctaatgcta acatattgt gacttagcg tggagactc cacatctgt ttgctgcgc acttagagc tgttacagt tttgtatac tattataat ttcagtaaa ttttttcta tataaaata taactgtag gtatttaag taatgaaaa gtttattta tcaatccta tgctttaag gcatgtgat catatttaa cgatagttt tatcaattc aggtacaga cctaagttt gaatatctg tgtctctga tagatatta aaaacaata gtccttggt tttattgta aatgtgctc tgttattta taattgaaa atttactac aatataata accgttatt tgctggttt tgttgattt ggtaaagct agacgtgga ctaccgttc cagaatcgg ggcaaacac ccgacagta ccattccag tgtattatt ttttcgcaa gttttgtga aagactgtt gcagtttgc tcaggttta ggaaggata gttgaagtt accaaaatt cgcgttaca aaatttgtt tttgttact gtctttagc tatattaaa gttaatagg tcagattgt acacctgta acaaaataa cggtctttg tatgtaata taagcttat tcgaaat
