Status
Please wait ...
Gene information
General information
TC004900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4093626..4107139
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004900
    iBeetle screen iB_03870

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004900 RA
      MNASIKAQT GGTTPDGDS YVCVPPGYC NNGGTPTTP 
      GIDPRIVTP GELCPSGTI PCYTQGPSS RCGERFVSP 
      VSTADGQAT PGGHPWQAY LRNSTHPQY AGSGVLIDN 
      YHVLTAAHK VYGDQNNPA NVIISMGVH NPSDLTSPN 
      VQTRTASAI RVHPNYDPQ HLINDIAIV RVSSPFSLS 
      QNNINSACL PTADASYTG QTCVVAGWG ETNFGVQDH 
      PTNPMKQVN LSPVDIATC RAGLLPVLP TVDTYLDMT 
      GGEICAGGE SMKDACTYD GGAPLTCPN TGKGNIAGL 
      VIWGKSCGQ PSVYGVYVS VPFYRAWID STITQLNNS
    • >TC004900 RB
      MHAKEASIK AQTGGTTPD GDSYVCVPP GYCNNGGTP 
      TTPGIDPRI VTPGELCPS GTIPCYTQG PSSRCGERF 
      VSPVSTADG QATPGGHPW QAYLRNSTH PQYAGSGVL 
      IDNYHVLTA AHKVYGDQN NPANVIISM GVHNPSDLT 
      SPNVQTRTA SAIRVHPNY DPQHLINDI AIVRVSSPF 
      SLSQNNINS ACLPTADAS YTGQTCVVA GWGETNFGV 
      QDHPTNPMK QVNLSPVDI ATCRAGLLP VLPTVDTYL 
      DMTGGEICA GGESMKDAC TYDGGAPLT CPNTGKGNI 
      AGLVIWGKS CGQPSVYGV YVSVPFYRA WIDSTITQL 
      NNS
    CDS sequence
    • >TC004900 RA
      atgaacgct tcaatcaaa gcacaaaca ggaggaacc 
      acacctgac ggtgactcc tacgtgtgc gtacctccg 
      ggatattgc aacaacggt ggcacccca acaactcca 
      gggatcgat ccccggatt gttacaccc ggagaactt 
      tgtccgagt ggaacaatc ccatgttac actcaaggg 
      ccatcgagc cgatgtggc gaaagattc gtgtctcct 
      gtttcaaca gccgatggg caagcaact ccaggcggt 
      cacccatgg caagcttac ctacgaaat tccacccat 
      ccacaatat gcgggcagt ggtgttcta atcgacaat 
      taccacgtt cttacagca gcacacaaa gtctacgga 
      gaccaaaat aatcccgcg aatgttatt ataagcatg 
      ggtgtgcac aatcccagt gatttaacg tcgcccaat 
      gttcagact cgaacagct tccgcgata agagttcat 
      ccaaattac gacccccag catttgatc aacgacatt 
      gcaatagtc agagtgtct tccccattt tctctaagt 
      caaaacaat atcaattct gcttgtctt ccgacagct 
      gacgcatca tacaccggt caaacatgt gttgtggct 
      ggatggggc gaaacaaat tttggcgtc caagaccat 
      cccactaat cccatgaaa caagtcaac ctatcacct 
      gttgatatt gccacttgt cgagcagga ttattgcca 
      gttttgcca acagttgac acgtatctg gatatgacc 
      ggaggagaa atttgcgca ggaggcgaa tcaatgaag 
      gatgcgtgc acgtacgac ggaggagca cctctcaca 
      tgtcccaac actgggaaa ggcaacatt gctggattg 
      gtcatttgg gggaaaagt tgcggacaa ccgagtgtc 
      tatggagtc tacgtaagc gtgccgttt tatagggca 
      tggatcgac agcaccata acacagctg aataattcg 
      taa
    • >TC004900 RB
      atgcacgcg aaagaagct tcaatcaaa gcacaaaca 
      ggaggaacc acacctgac ggtgactcc tacgtgtgc 
      gtacctccg ggatattgc aacaacggt ggcacccca 
      acaactcca gggatcgat ccccggatt gttacaccc 
      ggagaactt tgtccgagt ggaacaatc ccatgttac 
      actcaaggg ccatcgagc cgatgtggc gaaagattc 
      gtgtctcct gtttcaaca gccgatggg caagcaact 
      ccaggcggt cacccatgg caagcttac ctacgaaat 
      tccacccat ccacaatat gcgggcagt ggtgttcta 
      atcgacaat taccacgtt cttacagca gcacacaaa 
      gtctacgga gaccaaaat aatcccgcg aatgttatt 
      ataagcatg ggtgtgcac aatcccagt gatttaacg 
      tcgcccaat gttcagact cgaacagct tccgcgata 
      agagttcat ccaaattac gacccccag catttgatc 
      aacgacatt gcaatagtc agagtgtct tccccattt 
      tctctaagt caaaacaat atcaattct gcttgtctt 
      ccgacagct gacgcatca tacaccggt caaacatgt 
      gttgtggct ggatggggc gaaacaaat tttggcgtc 
      caagaccat cccactaat cccatgaaa caagtcaac 
      ctatcacct gttgatatt gccacttgt cgagcagga 
      ttattgcca gttttgcca acagttgac acgtatctg 
      gatatgacc ggaggagaa atttgcgca ggaggcgaa 
      tcaatgaag gatgcgtgc acgtacgac ggaggagca 
      cctctcaca tgtcccaac actgggaaa ggcaacatt 
      gctggattg gtcatttgg gggaaaagt tgcggacaa 
      ccgagtgtc tatggagtc tacgtaagc gtgccgttt 
      tatagggca tggatcgac agcaccata acacagctg 
      aataattcg taa
    mRNA sequence
    • >TC004900 RA
      ttagtaatg atgaacgct tcaatcaaa gcacaaaca 
      ggaggaacc acacctgac ggtgactcc tacgtgtgc 
      gtacctccg ggatattgc aacaacggt ggcacccca 
      acaactcca gggatcgat ccccggatt gttacaccc 
      ggagaactt tgtccgagt ggaacaatc ccatgttac 
      actcaaggg ccatcgagc cgatgtggc gaaagattc 
      gtgtctcct gtttcaaca gccgatggg caagcaact 
      ccaggcggt cacccatgg caagcttac ctacgaaat 
      tccacccat ccacaatat gcgggcagt ggtgttcta 
      atcgacaat taccacgtt cttacagca gcacacaaa 
      gtctacgga gaccaaaat aatcccgcg aatgttatt 
      ataagcatg ggtgtgcac aatcccagt gatttaacg 
      tcgcccaat gttcagact cgaacagct tccgcgata 
      agagttcat ccaaattac gacccccag catttgatc 
      aacgacatt gcaatagtc agagtgtct tccccattt 
      tctctaagt caaaacaat atcaattct gcttgtctt 
      ccgacagct gacgcatca tacaccggt caaacatgt 
      gttgtggct ggatggggc gaaacaaat tttggcgtc 
      caagaccat cccactaat cccatgaaa caagtcaac 
      ctatcacct gttgatatt gccacttgt cgagcagga 
      ttattgcca gttttgcca acagttgac acgtatctg 
      gatatgacc ggaggagaa atttgcgca ggaggcgaa 
      tcaatgaag gatgcgtgc acgtacgac ggaggagca 
      cctctcaca tgtcccaac actgggaaa ggcaacatt 
      gctggattg gtcatttgg gggaaaagt tgcggacaa 
      ccgagtgtc tatggagtc tacgtaagc gtgccgttt 
      tatagggca tggatcgac agcaccata acacagctg 
      aataattcg taataaagc gataatatt tatatgata 
      tttatttgt gaaaactgg actgaataa atcaactaa 
      attctt
    • >TC004900 RB
      gaccaggca tctctcggg cgttatcag caccggtcg 
      cgtccatag atcaaggaa gagcctatc gggggaaag 
      atattagag gctccatac aatgcagac attatgcac 
      gcgaaagaa gcttcaatc aaagcacaa acaggagga 
      accacacct gacggtgac tcctacgtg tgcgtacct 
      ccgggatat tgcaacaac ggtggcacc ccaacaact 
      ccagggatc gatccccgg attgttaca cccggagaa 
      ctttgtccg agtggaaca atcccatgt tacactcaa 
      gggccatcg agccgatgt ggcgaaaga ttcgtgtct 
      cctgtttca acagccgat gggcaagca actccaggc 
      ggtcaccca tggcaagct tacctacga aattccacc 
      catccacaa tatgcgggc agtggtgtt ctaatcgac 
      aattaccac gttcttaca gcagcacac aaagtctac 
      ggagaccaa aataatccc gcgaatgtt attataagc 
      atgggtgtg cacaatccc agtgattta acgtcgccc 
      aatgttcag actcgaaca gcttccgcg ataagagtt 
      catccaaat tacgacccc cagcatttg atcaacgac 
      attgcaata gtcagagtg tcttcccca ttttctcta 
      agtcaaaac aatatcaat tctgcttgt cttccgaca 
      gctgacgca tcatacacc ggtcaaaca tgtgttgtg 
      gctggatgg ggcgaaaca aattttggc gtccaagac 
      catcccact aatcccatg aaacaagtc aacctatca 
      cctgttgat attgccact tgtcgagca ggattattg 
      ccagttttg ccaacagtt gacacgtat ctggatatg 
      accggagga gaaatttgc gcaggaggc gaatcaatg 
      aaggatgcg tgcacgtac gacggagga gcacctctc 
      acatgtccc aacactggg aaaggcaac attgctgga 
      ttggtcatt tgggggaaa agttgcgga caaccgagt 
      gtctatgga gtctacgta agcgtgccg ttttatagg 
      gcatggatc gacagcacc ataacacag ctgaataat 
      tcgtaataa agcgataat atttatatg atatttatt 
      tgtgaaaac tggactgaa taaatcaac taaattctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY