Status
Please wait ...
Gene information
General information
TC004898 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4080908..4085185
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004898
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10

    maxillary palp size elongated - Number of animals showing the phenotype: 10

    Adult morphology
    antenna orientation bent - Number of animals showing the phenotype: 9


    maxillary palp size elongated - Number of animals showing the phenotype: 9


    Fertility
    egg production: number <50% of expected -
    Comment: No phenotype found!
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004898 RA
      MGTTVVCAV LFGAVFGVL SASLSKALR YQAPDECKW 
      VVVDSGTED DVALVCRLQ TINSELENT NFSVIQPQH 
      TVRLRLECS DALFFQSSL SAGSFKPLV ELRELSIEY 
      CKIGNLSDG AFRGLKELR NLTVRTHNT DWSAMTLEV 
      SPNAFTDEL RLLERLDLG ENNMWSLPD GVLCPLYAL 
      EVLNLTRNR LREVSSFRF AHNPSESCG SNLRLLDLS 
      RNNIDRLPS SQFSALSRL QKLYLQGNG LTHLADRAL 
      EGLVALNVL KLADNRLVS LPPELFSDT KDIREMYLQ 
      NNSINVLAP GLFSELTQL LVLDLSHNE LTADWINAA 
      TFAGLVRLV VLDISHNRI TKLEQSVFR DLYSLQILR 
      LNDNFIENI PENTFSALY NLHTLIISN NKITKIESD 
      TFNGLYVLS LLSLDNNRI SWIHQEALK NCSSLQDLH 
      LNGNKLVQV PEVLYSVPM LKTLDLGEN HIDVITNET 
      FRDMNQMYG LRLTENNIG NISKGVFDK MTALKILNL 
      SRNKIQKVA AGAFDANVN LQAIRLDGN YLTDIQELF 
      AKLPSLVWL NISDNQLKW FDYALIPTG LQWLDIHSN 
      QIEELGNFF EIESSLSLS TFDASANKL TEITGSAIP 
      NSVEVLFLN DNLISKVQS YTFFKKPNL TRVDLFGNK 
      ITSLDPNAL RISSVPPDK PLPEFYIGG NPYQCDCTM 
      EWLQKVNVG NRNRNQPKL VDLESIYCQ LLYNRGRTY 
      VPLVEAAPF QFLCTYETH CFALCHCCD FDACDCEMT 
      CPTNCTCYH DQSWSANVV DCSSAGYVS KLPEQIPMY 
      ATQLYLDGN DLRSLNSHA FIGRKKLKI LFVNNSNIE 
      VIQNRTFNG LKDLEILHL DENRIAELH GYEFEGLES 
      LREIFLQQN RITHINNTT FVGLRQLRI VRLDHNRLH 
      LYDVWQLPT TMIEVTLAY NPWSCECEF TEKLREWMV 
      RGDAVSDSA MVRCVFNTT EIDYYSEDV YSDNPETGF 
      YVNQENGTS CTGLPNIDN SVNGNLTAT KTIIQRQII 
      QDYLPLLVI TLAVFAFLI IVILLLFIF RQEVRVWLH 
      SKFGVRLFQ RSSDLDRDD RDKLFDAFV SYSSKDEAW 
      VAEVLAPAL EPNYKLCLH YRDFPVGAF LADTIVQAV 
      ESSKRTIMI LSENFIKSE WCRFEFKSA HHQVLRDRR 
      RRLIVVLLG EVPQKDLDP DIRLYLKTN VYLQWGDKL 
      FWEKLKFAL PDVPNNQRL RGSVRGPHV HHHVHRHST 
      RAQAPNPPG SGGAGGGAT RTVAIHI
    CDS sequence
    • >TC004898 RA
      atgggcacc acagtggtg tgtgcggtg ttattcggc 
      gccgtcttc ggcgtgctc tcggcgtcg ctgagcaag 
      gcgctgcgc taccaggcc ccagacgag tgcaagtgg 
      gtggtggtg gacagcggc accgaagac gacgtcgca 
      ctcgtctgt cgcttgcaa acaatcaac agtgaactg 
      gaaaacacc aacttcagt gtcatccag ccccagcac 
      accgtcaga ttgcggctc gaatgcagc gacgccttg 
      ttttttcaa agctcgtta agcgccggc agctttaag 
      ccgctagtc gaactccgg gagctttcc atcgagtac 
      tgcaagatc ggaaatctc tccgatgga gccttccgg 
      ggactgaaa gaactccgg aacctcaca gtacggact 
      cataacacg gattggtca gccatgacc ctcgaagtg 
      tcgccgaac gcgttcacg gacgagctg cgtctacta 
      gagcggctg gatctcggt gaaaacaac atgtggagt 
      ttaccggac ggtgtgctg tgccccttg tatgcgttg 
      gaagtgctc aatttgact cggaatcgg ttacgcgaa 
      gtgtcgagt tttcggttt gcccacaac ccgtcggaa 
      agctgcggc tccaacttg cgtcttctc gatctatca 
      cgtaacaac atagacagg cttccatcc agtcaattt 
      tccgcgctg tcacgtctt cagaaactt taccttcaa 
      ggaaacgga ctcacacat ttggcggat cgtgcactc 
      gaaggactc gtagcactc aacgtcctc aaattagcc 
      gacaaccga ttagtcagc ctaccacct gaattattt 
      tccgacacg aaagatatc agagaaatg tatttacaa 
      aataattca ataaatgtc ctagcacct ggacttttt 
      agtgagtta acccaatta ctcgtactg gatttatct 
      cacaacgaa ctcacagct gattggata aacgcagct 
      acttttgca ggcctagta cgccttgtc gttttagac 
      atttcccat aacagaata acgaaactg gaacaatca 
      gtatttcgt gacttgtat agtctccaa atccttcgc 
      ttgaatgat aattttatc gagaacata cctgaaaat 
      accttttca gctctttac aacctgcac acattaatt 
      atctccaac aataagata actaaaatt gaaagtgat 
      acgttcaat ggactttac gtgttatcg ctactgtca 
      ttagataat aacagaata tcatggata caccaagaa 
      gcactgaag aactgttcc agtttacaa gacctccac 
      cttaacggt aacaaatta gtgcaagtg cctgaagtg 
      ttatacagc gtacccatg ctgaaaacg ttagatcta 
      ggagagaat cacatcgac gttataacg aacgaaact 
      tttcgtgac atgaatcaa atgtacggt ctgcgattg 
      accgaaaat aacatcggc aacatatcg aaaggagtt 
      ttcgataaa atgacagcg ctgaaaatc ctgaacctt 
      tcccgcaat aaaatccaa aaagttgcg gcaggtgca 
      ttcgacgca aacgtcaat ttgcaagcg attcgtttg 
      gacggcaat tatttaacc gacattcag gaattgttc 
      gccaaatta ccgagttta gtttggctc aatatttcg 
      gacaatcaa ctgaaatgg ttcgactat gccctaata 
      ccaaccggc ctacaatgg ctcgatatc cactctaat 
      caaatcgaa gaattgggc aattttttc gaaatcgag 
      tcaagtctc tctctaagc acattcgac gccagcgcc 
      aacaagctg accgaaatc accggaagt gcaattccc 
      aacagcgta gaagtactg tttctcaac gacaattta 
      atttcaaaa gtacagtct tacacgttt ttcaaaaag 
      ccgaacttg actcgtgtc gatttattc gggaataaa 
      attacaagt ttggacccg aatgccctc aggatttcg 
      tcggtgcct ccggacaaa ccactgccg gaattttac 
      atcggcggc aacccgtat caatgcgac tgcacgatg 
      gaatggcta caaaaagtc aacgtcgga aaccgtaac 
      cgaaatcaa ccgaaatta gtggacttg gagagcatt 
      tactgccaa ttattgtat aatcggggc cgcacctat 
      gttcctctt gtagaggca gctcctttt cagttcctt 
      tgtacgtat gaaacgcac tgttttgca ttgtgtcac 
      tgctgtgat tttgacgcg tgtgattgt gaaatgaca 
      tgtccgacg aattgtacc tgctaccac gatcaatcc 
      tggtcagct aacgtcgtc gattgttcc tcagccggg 
      tacgtttcc aaattaccc gagcaaatt ccgatgtat 
      gcgacgcaa ctttatctc gacggtaac gatttacgt 
      tcattgaac agccacgca ttcatcggc cgaaaaaag 
      ctcaagatt ttattcgta aataattcc aacatcgaa 
      gtcatccaa aaccgcact ttcaacggc ctcaaagac 
      ttggaaata cttcattta gacgagaac aggatcgcc 
      gaactgcac ggctatgaa ttcgagggc ttggaaagt 
      ctccgagag attttcctt cagcaaaac cgtatcacg 
      catattaac aatacaact ttcgttgga cttcgacag 
      ctgcgtatt gttcggttg gaccacaac aggttgcat 
      ttgtacgac gtatggcaa ttaccaacc accatgatc 
      gaggtgact ctggcctac aacccctgg tcttgcgaa 
      tgcgaattt acggaaaaa ctccgcgag tggatggtg 
      cgaggtgac gcggtttcg gacagcgcg atggttcgt 
      tgtgttttc aacacaacc gaaatcgac tattacagt 
      gaggacgtt tattcggat aatcccgaa accgggttt 
      tacgtaaac caagagaac ggtacgtcg tgcacgggc 
      ctgccgaac atcgataac agtgtcaac ggtaatctg 
      acagcgacg aaaaccata atccaacgc caaataatc 
      caagattat ttgccgctc ctagtgata actctcgcc 
      gttttcgcg tttttgatc atcgtgatc ctcctgttg 
      ttcatcttc cgccaagaa gtgcgcgtt tggctgcat 
      tctaaattc ggagttcgc ctcttccag aggtcatcc 
      gacctggac cgcgacgac agagacaaa ttattcgac 
      gctttcgtt agttacagt tcaaaggac gaggcgtgg 
      gtcgccgag gtgttggcg cccgccctc gagcccaac 
      tacaaactg tgcctccac taccgcgac ttcccggtg 
      ggggccttc ctcgccgac accatcgtg caagcggtc 
      gagtcctcc aagcgcacc atcatgatc ctgtccgaa 
      aacttcatc aagtccgaa tggtgccgc ttcgagttc 
      aaatcggca caccaccaa gtgttgcgc gaccgccgg 
      cgccgcctc atcgtggtg ctcctcggg gaagtgccc 
      cagaaggac ctggacccc gacattcgg ctctatctc 
      aagactaac gtatacttg caatggggc gacaaactc 
      ttttgggag aagctcaaa ttcgctttg cccgacgtg 
      cctaacaac cagcgactt aggggctcc gtgaggggg 
      cctcacgtg caccaccac gtgcaccgg cacagcaca 
      cgggcccaa gcacccaac ccccctggg agcggcggt 
      gctggaggc ggcgccacc cggacagtg gccatccac 
      atatga
    mRNA sequence
    • >TC004898 RA
      gcaagaaga catcgttac gtccgcata caatggtgt 
      agtttttag tcatttaaa ctatgggca ccacagtgg 
      tgtgtgcgg tgttattcg gcgccgtct tcggcgtgc 
      tctcggcgt cgctgagca aggcgctgc gctaccagg 
      ccccagacg agtgcaagt gggtggtgg tggacagcg 
      gcaccgaag acgacgtcg cactcgtct gtcgcttgc 
      aaacaatca acagtgaac tggaaaaca ccaacttca 
      gtgtcatcc agccccagc acaccgtca gattgcggc 
      tcgaatgca gcgacgcct tgttttttc aaagctcgt 
      taagcgccg gcagcttta agccgctag tcgaactcc 
      gggagcttt ccatcgagt actgcaaga tcggaaatc 
      tctccgatg gagccttcc ggggactga aagaactcc 
      ggaacctca cagtacgga ctcataaca cggattggt 
      cagccatga ccctcgaag tgtcgccga acgcgttca 
      cggacgagc tgcgtctac tagagcggc tggatctcg 
      gtgaaaaca acatgtgga gtttaccgg acggtgtgc 
      tgtgcccct tgtatgcgt tggaagtgc tcaatttga 
      ctcggaatc ggttacgcg aagtgtcga gttttcggt 
      ttgcccaca acccgtcgg aaagctgcg gctccaact 
      tgcgtcttc tcgatctat cacgtaaca acatagaca 
      ggcttccat ccagtcaat tttccgcgc tgtcacgtc 
      ttcagaaac tttaccttc aaggaaacg gactcacac 
      atttggcgg atcgtgcac tcgaaggac tcgtagcac 
      tcaacgtcc tcaaattag ccgacaacc gattagtca 
      gcctaccac ctgaattat tttccgaca cgaaagata 
      tcagagaaa tgtatttac aaaataatt caataaatg 
      tcctagcac ctggacttt ttagtgagt taacccaat 
      tactcgtac tggatttat ctcacaacg aactcacag 
      ctgattgga taaacgcag ctacttttg caggcctag 
      tacgccttg tcgttttag acatttccc ataacagaa 
      taacgaaac tggaacaat cagtatttc gtgacttgt 
      atagtctcc aaatccttc gcttgaatg ataatttta 
      tcgagaaca tacctgaaa atacctttt cagctcttt 
      acaacctgc acacattaa ttatctcca acaataaga 
      taactaaaa ttgaaagtg atacgttca atggacttt 
      acgtgttat cgctactgt cattagata ataacagaa 
      tatcatgga tacaccaag aagcactga agaactgtt 
      ccagtttac aagacctcc accttaacg gtaacaaat 
      tagtgcaag tgcctgaag tgttataca gcgtaccca 
      tgctgaaaa cgttagatc taggagaga atcacatcg 
      acgttataa cgaacgaaa cttttcgtg acatgaatc 
      aaatgtacg gtctgcgat tgaccgaaa ataacatcg 
      gcaacatat cgaaaggag ttttcgata aaatgacag 
      cgctgaaaa tcctgaacc tttcccgca ataaaatcc 
      aaaaagttg cggcaggtg cattcgacg caaacgtca 
      atttgcaag cgattcgtt tggacggca attatttaa 
      ccgacattc aggaattgt tcgccaaat taccgagtt 
      tagtttggc tcaatattt cggacaatc aactgaaat 
      ggttcgact atgccctaa taccaaccg gcctacaat 
      ggctcgata tccactcta atcaaatcg aagaattgg 
      gcaattttt tcgaaatcg agtcaagtc tctctctaa 
      gcacattcg acgccagcg ccaacaagc tgaccgaaa 
      tcaccggaa gtgcaattc ccaacagcg tagaagtac 
      tgtttctca acgacaatt taatttcaa aagtacagt 
      cttacacgt ttttcaaaa agccgaact tgactcgtg 
      tcgatttat tcgggaata aaattacaa gtttggacc 
      cgaatgccc tcaggattt cgtcggtgc ctccggaca 
      aaccactgc cggaatttt acatcggcg gcaacccgt 
      atcaatgcg actgcacga tggaatggc tacaaaaag 
      tcaacgtcg gaaaccgta accgaaatc aaccgaaat 
      tagtggact tggagagca tttactgcc aattattgt 
      ataatcggg gccgcacct atgttcctc ttgtagagg 
      cagctcctt ttcagttcc tttgtacgt atgaaacgc 
      actgttttg cattgtgtc actgctgtg attttgacg 
      cgtgtgatt gtgaaatga catgtccga cgaattgta 
      cctgctacc acgatcaat cctggtcag ctaacgtcg 
      tcgattgtt cctcagccg ggtacgttt ccaaattac 
      ccgagcaaa ttccgatgt atgcgacgc aactttatc 
      tcgacggta acgatttac gttcattga acagccacg 
      cattcatcg gccgaaaaa agctcaaga ttttattcg 
      taaataatt ccaacatcg aagtcatcc aaaaccgca 
      ctttcaacg gcctcaaag acttggaaa tacttcatt 
      tagacgaga acaggatcg ccgaactgc acggctatg 
      aattcgagg gcttggaaa gtctccgag agattttcc 
      ttcagcaaa accgtatca cgcatatta acaatacaa 
      ctttcgttg gacttcgac agctgcgta ttgttcggt 
      tggaccaca acaggttgc atttgtacg acgtatggc 
      aattaccaa ccaccatga tcgaggtga ctctggcct 
      acaacccct ggtcttgcg aatgcgaat ttacggaaa 
      aactccgcg agtggatgg tgcgaggtg acgcggttt 
      cggacagcg cgatggttc gttgtgttt tcaacacaa 
      ccgaaatcg actattaca gtgaggacg tttattcgg 
      ataatcccg aaaccgggt tttacgtaa accaagaga 
      acggtacgt cgtgcacgg gcctgccga acatcgata 
      acagtgtca acggtaatc tgacagcga cgaaaacca 
      taatccaac gccaaataa tccaagatt atttgccgc 
      tcctagtga taactctcg ccgttttcg cgtttttga 
      tcatcgtga tcctcctgt tgttcatct tccgccaag 
      aagtgcgcg tttggctgc attctaaat tcggagttc 
      gcctcttcc agaggtcat ccgacctgg accgcgacg 
      acagagaca aattattcg acgctttcg ttagttaca 
      gttcaaagg acgaggcgt gggtcgccg aggtgttgg 
      cgcccgccc tcgagccca actacaaac tgtgcctcc 
      actaccgcg acttcccgg tgggggcct tcctcgccg 
      acaccatcg tgcaagcgg tcgagtcct ccaagcgca 
      ccatcatga tcctgtccg aaaacttca tcaagtccg 
      aatggtgcc gcttcgagt tcaaatcgg cacaccacc 
      aagtgttgc gcgaccgcc ggcgccgcc tcatcgtgg 
      tgctcctcg gggaagtgc cccagaagg acctggacc 
      ccgacattc ggctctatc tcaagacta acgtatact 
      tgcaatggg gcgacaaac tcttttggg agaagctca 
      aattcgctt tgcccgacg tgcctaaca accagcgac 
      ttaggggct ccgtgaggg ggcctcacg tgcaccacc 
      acgtgcacc ggcacagca cacgggccc aagcaccca 
      acccccctg ggagcggcg gtgctggag gcggcgcca 
      cccggacag tggccatcc acatatgac agtgatcct 
      aattaatgt caaccaatt gtgatacta cgtaataca 
      gactgtgag tgcaatgtg agtgccgga tgtgatagt 
      caaatgccg ggggtgtgt gtgtgcgag gctgcgacg 
      gccggactg accaaaaag ccacgggag aattgatga 
      atgagtgag tgtatggac cccggacac tccgacccg 
      gagtataac acttgttgc tgtatataa ggacttgta 
      tagctaagt atgttcaaa actgtatgt aaaactgta 
      aatatttat atatttttt atattcgat atttattgt 
      tgtaagttg tctacgtct tctcaaatt acttgccaa 
      ctctgcctt ttgtaccat cttttataa tgcaatttc 
      atatttttt aaagaccgt accgtaggt aaattctat 
      ttataaagt aacatataa tgccttttt tactgtgaa 
      tataagaaa taataaaat ctgaacact gat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY