Status
Please wait ...
Gene information
General information
TC004895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1782529..1804920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004895
    iBeetle screen iB_03868
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004895 RA
      MAVFSGLFV LCLVRCVFG TETLPTDSS APLRYEAPD 
      DCQWWIRES AVSGGQDEV ALTCNLRTI NSEFDTTNF 
      SVIPSEHTT SLRIECNAE IMSRSSLDD RSFVHLTKL 
      RELELEHCK LGRWPAGTL LGLRDLRNL TVRTHNTDW 
      PAMNLDFAK DSFMPVRQL ERLDLSCNN IWSFPENIF 
      CPLINLVAL NVSENRLQD VSDLGFREK APGLPQPLI 
      SVSDDDQPR NAETTRSFA HPCSLDIQV LDTSFNHFV 
      LIPANGFSA LRRLRELYI HNNEISMVA DRAIAGLKA 
      LQIFDLSNN KVVALPSEL FKDCSNAIK EIYLQNNSI 
      SVLAPGLFS NLEQLLALD LSKNLLSSS WINSETFAG 
      LIRLVLLNL SYNRITKLE PAFFRDLYT LQILNLEHN 
      SLETIPADT FAPMNNLHT LIISYNKIT YLDAYSLNG 
      LYVLSLLAL DNNLLEGIH PEAFRNCSS LQDLNLNGN 
      SLKAVPLAL KDMRLLRTV DLGENLISN LEEPGFRGM 
      SNLYGLRLI GNQLQNISK RAFNDLPAL QILNLARNK 
      IQRIEHGAF ESSPNLQAI RLDDNVLTD VSGLFANIP 
      SLLWLNISD NQIEWFDYA LIPKGLQWL DVHKNKVRE 
      LANRNNLEL QLRLQTLDA SFNKLTRIN AAAVPNSIE 
      LLFLNDNLI TTVEPHTFL KKTNLTRVD LYANQIVSM 
      DLNALRLTP VDPERPLPE FYIGGNPYQ CDCTMEWLQ 
      RINKLDHLR QHPRVMDLE SIYCKLLYN RDSNYLPLI 
      DAESSQFLC TYKTHCFAL CHCCDFDAC DCEMTCPTN 
      CTCYHDQSW SANVVECSG VGYTEMPSR IPMDATEVY 
      LDGNNFGEL SSHSFIGRK NLKILYANN SNIAAIYNH 
      TFSGLRRLT ILHLENNKI KELLGFELA SLENLRELY 
      LQGNMIHYI DNRTFLELK QLEVLRLDG NRLNSFEVW 
      QFALNPYLV EIGLGENQW SCECHYVEK FKSWFKVNH 
      GKVLDAGKV VCMFNNVTK AVGPHMGDF NATACAAYT 
      GGTRAIVET QVMNDYLPL LLVTMCVFL ASVVIVCGA 
      FYYRRELRV WIYSRCGLR MCYKTTAFE EQQDKDRLF 
      DAYVSYSVK DEAFVSQVL APGLESGDP GYRLCLHYR 
      DFNVSAYVA DTIIEAVES SRRTIIVLS KNFLQNEWC 
      RFEFKSALH EVLKDRRRR LIFVVTGEL PQRDLDPDL 
      RLYLKTNTV IEWGDRQFW QKLQFAMPD VRRPCLHQR 
      SANIYASAT PSRSPSAKR RSQHAPSSV GVADVQPQR 
      STAPACVFD ELNSEMDGL SLAPEVTVI VIFL
    CDS sequence
    • >TC004895 RA
      atggcggtc ttttccgga cttttcgtg ttgtgttta 
      gtgagatgt gtgtttggg acggagacg ctgccgacg 
      gactcgtcg gcacccctt cgatatgaa gctccagat 
      gattgccag tggtggatc cgggagtct gccgtgtct 
      ggcgggcaa gacgaagtg gccttaacg tgtaatttg 
      aggaccatc aacagtgaa ttcgacacg accaatttc 
      agtgtcatc ccatccgaa cacaccact tccctccgg 
      atcgagtgc aatgcggaa attatgtcc cggagtagc 
      ttagacgac cggagtttt gtccatttg acgaaatta 
      agggaactg gagctggag cattgtaaa ttgggcaga 
      tggccggct ggtacgtta ttaggactg agggatttg 
      aggaatttg acggtgagg acgcacaac accgattgg 
      ccggcgatg aatttggat tttgcgaaa gacagcttt 
      atgccggtt cgacaattg gagaggttg gatttgagt 
      tgtaataac atttggtcg tttccggag aacattttt 
      tgtcctttg atcaacttg gtggctttg aatgtcagt 
      gaaaataga ctgcaagat gtgagcgat ttgggcttt 
      cgggaaaaa gctcccgga cttccacaa ccgctgatt 
      agtgtgtct gatgatgat cagcctcgt aatgctgaa 
      accacacgc tcgtttgca catccgtgc agtttagac 
      attcaggta cttgatact tcatttaat catttcgtt 
      ttgattccg gctaacgga ttcagcgca ctccggcga 
      ctccgggag ctttatatt cacaataac gagatttcg 
      atggtggcg gatcgtgcc atcgccgga ctcaaagcg 
      ctacaaata ttcgatttg tctaataat aaagtagtg 
      gctttacca tccgaactt tttaaagac tgttccaat 
      gccataaaa gagatttat ttgcaaaac aattcgatt 
      agtgtgctc gctcccgga ctgttttcg aatttggag 
      caattgttg gcgttggac ttgtcgaaa aatctttta 
      tcaagttcc tggatcaac tcggagaca ttcgccggt 
      ttgatccgc ttagtactt ttgaacttg tcatataat 
      cgcataacg aaattagag ccggctttc tttagggac 
      ctgtacacg cttcaaatc ctcaattta gaacataat 
      tccttagaa actattcca gccgacacg tttgctccc 
      atgaataac ttgcacacg ttaattatt tcctataat 
      aaaatcacg tacctcgac gcatactcg ctaaacggg 
      ctgtacgtt ctgtctctg ctggcgctt gataataat 
      ctcctcgag ggaattcat cccgaagct ttcagaaac 
      tgttccagt ttgcaggac ttgaatttg aacggtaac 
      tcactgaaa gccgtgcct ctagcactc aaagatatg 
      cgtttactt cgtacagtg gatttaggc gaaaacttg 
      atctctaac ctcgaagag cccggtttc cgaggcatg 
      agcaacttg tacggcttg aggttaatc ggtaatcaa 
      cttcagaac atcagtaaa cgcgcattt aacgattta 
      ccagcacta caaatttta aatctcgcc cgcaataaa 
      atccagaga atagagcac ggagctttc gaatcgagt 
      cccaacctc caggcgata cgtctcgac gacaacgtt 
      ttaacggac gttagcggt cttttcgcc aatattccc 
      agtttatta tggctaaac atatccgat aaccaaatc 
      gaatggttc gattacgcc ctcatcccc aaaggactg 
      caatggctc gatgtccac aaaaacaaa gtccgggaa 
      cttgccaat cgcaacaac ttggaacta caattacgc 
      ttgcagact ttagacgcc agttttaac aaactaacg 
      aggattaat gccgcggcc gtgcccaac agcatcgag 
      ttattattt ctaaacgac aatcttata accacagtc 
      gaaccacac actttccta aagaaaaca aacctgacg 
      agagtggat ttatacgca aatcagatt gttagtatg 
      gatctgaac gctttacgc ttaactccg gtcgatccg 
      gaaagaccg ttgcccgaa ttttatatc ggaggtaat 
      ccgtatcag tgcgattgc accatggag tggctgcaa 
      cgcatcaat aaattggat catttgcgc caacaccct 
      cgtgtcatg gatttggaa agtatttat tgcaagctt 
      ctgtacaat cgcgatagc aactatctc ccgttgatc 
      gatgccgaa tcgtcgcag ttcctctgc acttacaag 
      acgcattgt tttgctctt tgccattgt tgcgacttt 
      gacgcctgt gattgcgaa atgacgtgt cccacaaac 
      tgtacttgc tatcacgat caatcctgg tcggccaac 
      gtcgtcgaa tgttccggt gtgggctac acagaaatg 
      cctagtcga atccccatg gacgctact gaagtctac 
      ttagacgga aacaatttt ggcgagttg tcgagtcac 
      tcgttcatt gggcgaaaa aacctaaag attttgtac 
      gccaacaat tcgaacatt gctgcgatt tacaatcac 
      acgtttagt ggcttgcgc cgcttaacc atcttgcac 
      ttggagaat aacaaaatc aaggagttg ttggggttc 
      gaattggcg agtctggaa aatttgagg gagctttat 
      ttgcaagga aatatgatc cactacatt gacaatagg 
      acgttcctg gaattgaag caattggag gtgttacgt 
      ttggacggg aatcgcttg aattctttc gaggtgtgg 
      caatttgca ttgaatcca tatttggtc gaaatagga 
      ttaggggaa aatcagtgg tcttgcgaa tgccactac 
      gtggagaaa tttaagtcg tggtttaag gtaaaccac 
      gggaaagtg ctcgatgct ggtaaagta gtttgtatg 
      tttaacaac gtaacgaaa gctgtgggc ccccacatg 
      ggcgatttc aacgcaacc gcttgtgct gcctacacc 
      gggggcacc cgagccatc gtggaaacc caagttatg 
      aacgactat ttgccttta ctcctcgtc acaatgtgc 
      gttttcctg gccagcgtt gtgatcgtt tgtggcgcc 
      ttttactac cgtcgcgag ttacgcgtg tggatatac 
      tctcggtgc ggcttgcga atgtgctac aagaccaca 
      gctttcgaa gagcagcaa gacaaagac cgccttttc 
      gacgcctac gtcagctac agcgtcaag gacgaagcc 
      ttcgtcagc caggtcctg gctccgggc ctggagtcc 
      ggcgatccg ggctaccgc ttgtgtttg cactaccgg 
      gactttaac gtgtccgcg tacgtcgcc gacacgata 
      atcgaagcg gtggagtcg tcccgacgc acaattatc 
      gtcctttcg aaaaacttt ctccagaac gagtggtgt 
      cgcttcgag ttcaagtcc gccctgcac gaagtgttg 
      aaagaccgg aggcgacgc ctcattttc gtggtcact 
      ggcgagttg ccccagcgg gatttggac ccggacttg 
      cggttgtac ttgaagacg aacacagtg atcgagtgg 
      ggggaccgc cagttctgg caaaaactc cagtttgcg 
      atgccggac gtgcgaagg ccgtgcttg caccagagg 
      tcggccaat atttacgct tcggcgacg ccgtcaagg 
      agtccgtct gccaagaga cgctcgcaa catgcccca 
      tcctctgtg ggcgtagcg gatgttcag ccgcagcga 
      tcgacagct ccagcgtgc gtttttgac gaattaaat 
      tcggagatg gacggtctt tccttggct cccgaggtg 
      actgtgatt gtgatattt ttgtga
    mRNA sequence
    • >TC004895 RA
      atcttcgga gagaggtgg tagtggtcc acgttcaca 
      ttccgggcg tgaacagca cttttcagc cagttgggg 
      caacatgac actgctctc gttaattac ttcacgtgc 
      ttcctgatg cggtgtcgc aagtggggt gcgagtcga 
      cagttccgt tgcgaccac cctaacgca cgcaccatg 
      agttaagac tgactcagt ctaagagtt gaacgatgg 
      cggtctttt ccggacttt tcgtgttgt gtttagtga 
      gatgtgtgt ttgggacgg agacgctgc cgacggact 
      cgtcggcac cccttcgat atgaagctc cagatgatt 
      gccagtggt ggatccggg agtctgccg tgtctggcg 
      ggcaagacg aagtggcct taacgtgta atttgagga 
      ccatcaaca gtgaattcg acacgacca atttcagtg 
      tcatcccat ccgaacaca ccacttccc tccggatcg 
      agtgcaatg cggaaatta tgtcccgga gtagcttag 
      acgaccgga gttttgtcc atttgacga aattaaggg 
      aactggagc tggagcatt gtaaattgg gcagatggc 
      cggctggta cgttattag gactgaggg atttgagga 
      atttgacgg tgaggacgc acaacaccg attggccgg 
      cgatgaatt tggattttg cgaaagaca gctttatgc 
      cggttcgac aattggaga ggttggatt tgagttgta 
      ataacattt ggtcgtttc cggagaaca ttttttgtc 
      ctttgatca acttggtgg ctttgaatg tcagtgaaa 
      atagactgc aagatgtga gcgatttgg gctttcggg 
      aaaaagctc ccggacttc cacaaccgc tgattagtg 
      tgtctgatg atgatcagc ctcgtaatg ctgaaacca 
      cacgctcgt ttgcacatc cgtgcagtt tagacattc 
      aggtacttg atacttcat ttaatcatt tcgttttga 
      ttccggcta acggattca gcgcactcc ggcgactcc 
      gggagcttt atattcaca ataacgaga tttcgatgg 
      tggcggatc gtgccatcg ccggactca aagcgctac 
      aaatattcg atttgtcta ataataaag tagtggctt 
      taccatccg aacttttta aagactgtt ccaatgcca 
      taaaagaga tttatttgc aaaacaatt cgattagtg 
      tgctcgctc ccggactgt tttcgaatt tggagcaat 
      tgttggcgt tggacttgt cgaaaaatc ttttatcaa 
      gttcctgga tcaactcgg agacattcg ccggtttga 
      tccgcttag tacttttga acttgtcat ataatcgca 
      taacgaaat tagagccgg ctttcttta gggacctgt 
      acacgcttc aaatcctca atttagaac ataattcct 
      tagaaacta ttccagccg acacgtttg ctcccatga 
      ataacttgc acacgttaa ttatttcct ataataaaa 
      tcacgtacc tcgacgcat actcgctaa acgggctgt 
      acgttctgt ctctgctgg cgcttgata ataatctcc 
      tcgagggaa ttcatcccg aagctttca gaaactgtt 
      ccagtttgc aggacttga atttgaacg gtaactcac 
      tgaaagccg tgcctctag cactcaaag atatgcgtt 
      tacttcgta cagtggatt taggcgaaa acttgatct 
      ctaacctcg aagagcccg gtttccgag gcatgagca 
      acttgtacg gcttgaggt taatcggta atcaacttc 
      agaacatca gtaaacgcg catttaacg atttaccag 
      cactacaaa ttttaaatc tcgcccgca ataaaatcc 
      agagaatag agcacggag ctttcgaat cgagtccca 
      acctccagg cgatacgtc tcgacgaca acgttttaa 
      cggacgtta gcggtcttt tcgccaata ttcccagtt 
      tattatggc taaacatat ccgataacc aaatcgaat 
      ggttcgatt acgccctca tccccaaag gactgcaat 
      ggctcgatg tccacaaaa acaaagtcc gggaacttg 
      ccaatcgca acaacttgg aactacaat tacgcttgc 
      agactttag acgccagtt ttaacaaac taacgagga 
      ttaatgccg cggccgtgc ccaacagca tcgagttat 
      tatttctaa acgacaatc ttataacca cagtcgaac 
      cacacactt tcctaaaga aaacaaacc tgacgagag 
      tggatttat acgcaaatc agattgtta gtatggatc 
      tgaacgctt tacgcttaa ctccggtcg atccggaaa 
      gaccgttgc ccgaatttt atatcggag gtaatccgt 
      atcagtgcg attgcacca tggagtggc tgcaacgca 
      tcaataaat tggatcatt tgcgccaac accctcgtg 
      tcatggatt tggaaagta tttattgca agcttctgt 
      acaatcgcg atagcaact atctcccgt tgatcgatg 
      ccgaatcgt cgcagttcc tctgcactt acaagacgc 
      attgttttg ctctttgcc attgttgcg actttgacg 
      cctgtgatt gcgaaatga cgtgtccca caaactgta 
      cttgctatc acgatcaat cctggtcgg ccaacgtcg 
      tcgaatgtt ccggtgtgg gctacacag aaatgccta 
      gtcgaatcc ccatggacg ctactgaag tctacttag 
      acggaaaca attttggcg agttgtcga gtcactcgt 
      tcattgggc gaaaaaacc taaagattt tgtacgcca 
      acaattcga acattgctg cgatttaca atcacacgt 
      ttagtggct tgcgccgct taaccatct tgcacttgg 
      agaataaca aaatcaagg agttgttgg ggttcgaat 
      tggcgagtc tggaaaatt tgagggagc tttatttgc 
      aaggaaata tgatccact acattgaca ataggacgt 
      tcctggaat tgaagcaat tggaggtgt tacgtttgg 
      acgggaatc gcttgaatt ctttcgagg tgtggcaat 
      ttgcattga atccatatt tggtcgaaa taggattag 
      gggaaaatc agtggtctt gcgaatgcc actacgtgg 
      agaaattta agtcgtggt ttaaggtaa accacggga 
      aagtgctcg atgctggta aagtagttt gtatgttta 
      acaacgtaa cgaaagctg tgggccccc acatgggcg 
      atttcaacg caaccgctt gtgctgcct acaccgggg 
      gcacccgag ccatcgtgg aaacccaag ttatgaacg 
      actatttgc ctttactcc tcgtcacaa tgtgcgttt 
      tcctggcca gcgttgtga tcgtttgtg gcgcctttt 
      actaccgtc gcgagttac gcgtgtgga tatactctc 
      ggtgcggct tgcgaatgt gctacaaga ccacagctt 
      tcgaagagc agcaagaca aagaccgcc ttttcgacg 
      cctacgtca gctacagcg tcaaggacg aagccttcg 
      tcagccagg tcctggctc cgggcctgg agtccggcg 
      atccgggct accgcttgt gtttgcact accgggact 
      ttaacgtgt ccgcgtacg tcgccgaca cgataatcg 
      aagcggtgg agtcgtccc gacgcacaa ttatcgtcc 
      tttcgaaaa actttctcc agaacgagt ggtgtcgct 
      tcgagttca agtccgccc tgcacgaag tgttgaaag 
      accggaggc gacgcctca ttttcgtgg tcactggcg 
      agttgcccc agcgggatt tggacccgg acttgcggt 
      tgtacttga agacgaaca cagtgatcg agtgggggg 
      accgccagt tctggcaaa aactccagt ttgcgatgc 
      cggacgtgc gaaggccgt gcttgcacc agaggtcgg 
      ccaatattt acgcttcgg cgacgccgt caaggagtc 
      cgtctgcca agagacgct cgcaacatg ccccatcct 
      ctgtgggcg tagcggatg ttcagccgc agcgatcga 
      cagctccag cgtgcgttt ttgacgaat taaattcgg 
      agatggacg gtctttcct tggctcccg aggtgactg 
      tgattgtga tatttttgt gaactttaa ttattctaa 
      atagatggc ccattcgtg tgaacgggc gcgtttcta 
      gttaattat tgttgttat aatttataa acaaaggac 
      tgaaaaagt ttgaatcga atttaacta aaaaagtta 
      taaagtcag tcttgatat atcaatgtt ctttacata 
      ctgcttcat gagcttatt attaatagg tgtattgaa 
      agaaattct catgtatta taaatattt caattagtt 
      caaacagta ctgtgacgt ttcatttat cgatcagct 
      agtaaggtt tttctaact ttaaacggg ttttacgtt 
      gttttatac gccgattat tattattta tttgccgcg 
      tattacaga aaggttgtg atttgtgta tttcctaca 
      gaacttgtt attagtatt ttaacagta tccacaacg 
      atattgata gacttaagt atcttgatt ggacaagtc 
      gacatttga tcgttccgt tttggtcaa acaccaatt 
      tttacaaaa tcaataagt catcaccaa attattttt 
      agttttgat gccactgga aacgaagcg atcaagtgg 
      aggacatcc atgatttac gattcctaa cttaatata 
      ttgtaatat tattaattg tatatactg tgtataaat 
      aatttaaag gtatatttt catcagaaa tcccaaagc 
      acactatgt aaatagcat tattatttg cttatttgg 
      aacgtaact taaaacaaa taaaattat ttaatttta