| General information | |||
| TC004895 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 3872262..3894653 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004895 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03868 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004895 RA
MAVFSGLFV LCLVRCVFG TETLPTDSS APLRYEAPD DCQWWIRES AVSGGQDEV ALTCNLRTI NSEFDTTNF SVIPSEHTT SLRIECNAE IMSRSSLDD RSFVHLTKL RELELEHCK LGRWPAGTL LGLRDLRNL TVRTHNTDW PAMNLDFAK DSFMPVRQL ERLDLSCNN IWSFPENIF CPLINLVAL NVSENRLQD VSDLGFREK APGLPQPLI SVSDDDQPR NAETTRSFA HPCSLDIQV LDTSFNHFV LIPANGFSA LRRLRELYI HNNEISMVA DRAIAGLKA LQIFDLSNN KVVALPSEL FKDCSNAIK EIYLQNNSI SVLAPGLFS NLEQLLALD LSKNLLSSS WINSETFAG LIRLVLLNL SYNRITKLE PAFFRDLYT LQILNLEHN SLETIPADT FAPMNNLHT LIISYNKIT YLDAYSLNG LYVLSLLAL DNNLLEGIH PEAFRNCSS LQDLNLNGN SLKAVPLAL KDMRLLRTV DLGENLISN LEEPGFRGM SNLYGLRLI GNQLQNISK RAFNDLPAL QILNLARNK IQRIEHGAF ESSPNLQAI RLDDNVLTD VSGLFANIP SLLWLNISD NQIEWFDYA LIPKGLQWL DVHKNKVRE LANRNNLEL QLRLQTLDA SFNKLTRIN AAAVPNSIE LLFLNDNLI TTVEPHTFL KKTNLTRVD LYANQIVSM DLNALRLTP VDPERPLPE FYIGGNPYQ CDCTMEWLQ RINKLDHLR QHPRVMDLE SIYCKLLYN RDSNYLPLI DAESSQFLC TYKTHCFAL CHCCDFDAC DCEMTCPTN CTCYHDQSW SANVVECSG VGYTEMPSR IPMDATEVY LDGNNFGEL SSHSFIGRK NLKILYANN SNIAAIYNH TFSGLRRLT ILHLENNKI KELLGFELA SLENLRELY LQGNMIHYI DNRTFLELK QLEVLRLDG NRLNSFEVW QFALNPYLV EIGLGENQW SCECHYVEK FKSWFKVNH GKVLDAGKV VCMFNNVTK AVGPHMGDF NATACAAYT GGTRAIVET QVMNDYLPL LLVTMCVFL ASVVIVCGA FYYRRELRV WIYSRCGLR MCYKTTAFE EQQDKDRLF DAYVSYSVK DEAFVSQVL APGLESGDP GYRLCLHYR DFNVSAYVA DTIIEAVES SRRTIIVLS KNFLQNEWC RFEFKSALH EVLKDRRRR LIFVVTGEL PQRDLDPDL RLYLKTNTV IEWGDRQFW QKLQFAMPD VRRPCLHQR SANIYASAT PSRSPSAKR RSQHAPSSV GVADVQPQR STAPACVFD ELNSEMDGL SLAPEVTVI VIFL
- >TC004895 RA
atggcggtc ttttccgga cttttcgtg ttgtgttta gtgagatgt gtgtttggg acggagacg ctgccgacg gactcgtcg gcacccctt cgatatgaa gctccagat gattgccag tggtggatc cgggagtct gccgtgtct ggcgggcaa gacgaagtg gccttaacg tgtaatttg aggaccatc aacagtgaa ttcgacacg accaatttc agtgtcatc ccatccgaa cacaccact tccctccgg atcgagtgc aatgcggaa attatgtcc cggagtagc ttagacgac cggagtttt gtccatttg acgaaatta agggaactg gagctggag cattgtaaa ttgggcaga tggccggct ggtacgtta ttaggactg agggatttg aggaatttg acggtgagg acgcacaac accgattgg ccggcgatg aatttggat tttgcgaaa gacagcttt atgccggtt cgacaattg gagaggttg gatttgagt tgtaataac atttggtcg tttccggag aacattttt tgtcctttg atcaacttg gtggctttg aatgtcagt gaaaataga ctgcaagat gtgagcgat ttgggcttt cgggaaaaa gctcccgga cttccacaa ccgctgatt agtgtgtct gatgatgat cagcctcgt aatgctgaa accacacgc tcgtttgca catccgtgc agtttagac attcaggta cttgatact tcatttaat catttcgtt ttgattccg gctaacgga ttcagcgca ctccggcga ctccgggag ctttatatt cacaataac gagatttcg atggtggcg gatcgtgcc atcgccgga ctcaaagcg ctacaaata ttcgatttg tctaataat aaagtagtg gctttacca tccgaactt tttaaagac tgttccaat gccataaaa gagatttat ttgcaaaac aattcgatt agtgtgctc gctcccgga ctgttttcg aatttggag caattgttg gcgttggac ttgtcgaaa aatctttta tcaagttcc tggatcaac tcggagaca ttcgccggt ttgatccgc ttagtactt ttgaacttg tcatataat cgcataacg aaattagag ccggctttc tttagggac ctgtacacg cttcaaatc ctcaattta gaacataat tccttagaa actattcca gccgacacg tttgctccc atgaataac ttgcacacg ttaattatt tcctataat aaaatcacg tacctcgac gcatactcg ctaaacggg ctgtacgtt ctgtctctg ctggcgctt gataataat ctcctcgag ggaattcat cccgaagct ttcagaaac tgttccagt ttgcaggac ttgaatttg aacggtaac tcactgaaa gccgtgcct ctagcactc aaagatatg cgtttactt cgtacagtg gatttaggc gaaaacttg atctctaac ctcgaagag cccggtttc cgaggcatg agcaacttg tacggcttg aggttaatc ggtaatcaa cttcagaac atcagtaaa cgcgcattt aacgattta ccagcacta caaatttta aatctcgcc cgcaataaa atccagaga atagagcac ggagctttc gaatcgagt cccaacctc caggcgata cgtctcgac gacaacgtt ttaacggac gttagcggt cttttcgcc aatattccc agtttatta tggctaaac atatccgat aaccaaatc gaatggttc gattacgcc ctcatcccc aaaggactg caatggctc gatgtccac aaaaacaaa gtccgggaa cttgccaat cgcaacaac ttggaacta caattacgc ttgcagact ttagacgcc agttttaac aaactaacg aggattaat gccgcggcc gtgcccaac agcatcgag ttattattt ctaaacgac aatcttata accacagtc gaaccacac actttccta aagaaaaca aacctgacg agagtggat ttatacgca aatcagatt gttagtatg gatctgaac gctttacgc ttaactccg gtcgatccg gaaagaccg ttgcccgaa ttttatatc ggaggtaat ccgtatcag tgcgattgc accatggag tggctgcaa cgcatcaat aaattggat catttgcgc caacaccct cgtgtcatg gatttggaa agtatttat tgcaagctt ctgtacaat cgcgatagc aactatctc ccgttgatc gatgccgaa tcgtcgcag ttcctctgc acttacaag acgcattgt tttgctctt tgccattgt tgcgacttt gacgcctgt gattgcgaa atgacgtgt cccacaaac tgtacttgc tatcacgat caatcctgg tcggccaac gtcgtcgaa tgttccggt gtgggctac acagaaatg cctagtcga atccccatg gacgctact gaagtctac ttagacgga aacaatttt ggcgagttg tcgagtcac tcgttcatt gggcgaaaa aacctaaag attttgtac gccaacaat tcgaacatt gctgcgatt tacaatcac acgtttagt ggcttgcgc cgcttaacc atcttgcac ttggagaat aacaaaatc aaggagttg ttggggttc gaattggcg agtctggaa aatttgagg gagctttat ttgcaagga aatatgatc cactacatt gacaatagg acgttcctg gaattgaag caattggag gtgttacgt ttggacggg aatcgcttg aattctttc gaggtgtgg caatttgca ttgaatcca tatttggtc gaaatagga ttaggggaa aatcagtgg tcttgcgaa tgccactac gtggagaaa tttaagtcg tggtttaag gtaaaccac gggaaagtg ctcgatgct ggtaaagta gtttgtatg tttaacaac gtaacgaaa gctgtgggc ccccacatg ggcgatttc aacgcaacc gcttgtgct gcctacacc gggggcacc cgagccatc gtggaaacc caagttatg aacgactat ttgccttta ctcctcgtc acaatgtgc gttttcctg gccagcgtt gtgatcgtt tgtggcgcc ttttactac cgtcgcgag ttacgcgtg tggatatac tctcggtgc ggcttgcga atgtgctac aagaccaca gctttcgaa gagcagcaa gacaaagac cgccttttc gacgcctac gtcagctac agcgtcaag gacgaagcc ttcgtcagc caggtcctg gctccgggc ctggagtcc ggcgatccg ggctaccgc ttgtgtttg cactaccgg gactttaac gtgtccgcg tacgtcgcc gacacgata atcgaagcg gtggagtcg tcccgacgc acaattatc gtcctttcg aaaaacttt ctccagaac gagtggtgt cgcttcgag ttcaagtcc gccctgcac gaagtgttg aaagaccgg aggcgacgc ctcattttc gtggtcact ggcgagttg ccccagcgg gatttggac ccggacttg cggttgtac ttgaagacg aacacagtg atcgagtgg ggggaccgc cagttctgg caaaaactc cagtttgcg atgccggac gtgcgaagg ccgtgcttg caccagagg tcggccaat atttacgct tcggcgacg ccgtcaagg agtccgtct gccaagaga cgctcgcaa catgcccca tcctctgtg ggcgtagcg gatgttcag ccgcagcga tcgacagct ccagcgtgc gtttttgac gaattaaat tcggagatg gacggtctt tccttggct cccgaggtg actgtgatt gtgatattt ttgtga
- >TC004895 RA
atcttcgga gagaggtgg tagtggtcc acgttcaca ttccgggcg tgaacagca cttttcagc cagttgggg caacatgac actgctctc gttaattac ttcacgtgc ttcctgatg cggtgtcgc aagtggggt gcgagtcga cagttccgt tgcgaccac cctaacgca cgcaccatg agttaagac tgactcagt ctaagagtt gaacgatgg cggtctttt ccggacttt tcgtgttgt gtttagtga gatgtgtgt ttgggacgg agacgctgc cgacggact cgtcggcac cccttcgat atgaagctc cagatgatt gccagtggt ggatccggg agtctgccg tgtctggcg ggcaagacg aagtggcct taacgtgta atttgagga ccatcaaca gtgaattcg acacgacca atttcagtg tcatcccat ccgaacaca ccacttccc tccggatcg agtgcaatg cggaaatta tgtcccgga gtagcttag acgaccgga gttttgtcc atttgacga aattaaggg aactggagc tggagcatt gtaaattgg gcagatggc cggctggta cgttattag gactgaggg atttgagga atttgacgg tgaggacgc acaacaccg attggccgg cgatgaatt tggattttg cgaaagaca gctttatgc cggttcgac aattggaga ggttggatt tgagttgta ataacattt ggtcgtttc cggagaaca ttttttgtc ctttgatca acttggtgg ctttgaatg tcagtgaaa atagactgc aagatgtga gcgatttgg gctttcggg aaaaagctc ccggacttc cacaaccgc tgattagtg tgtctgatg atgatcagc ctcgtaatg ctgaaacca cacgctcgt ttgcacatc cgtgcagtt tagacattc aggtacttg atacttcat ttaatcatt tcgttttga ttccggcta acggattca gcgcactcc ggcgactcc gggagcttt atattcaca ataacgaga tttcgatgg tggcggatc gtgccatcg ccggactca aagcgctac aaatattcg atttgtcta ataataaag tagtggctt taccatccg aacttttta aagactgtt ccaatgcca taaaagaga tttatttgc aaaacaatt cgattagtg tgctcgctc ccggactgt tttcgaatt tggagcaat tgttggcgt tggacttgt cgaaaaatc ttttatcaa gttcctgga tcaactcgg agacattcg ccggtttga tccgcttag tacttttga acttgtcat ataatcgca taacgaaat tagagccgg ctttcttta gggacctgt acacgcttc aaatcctca atttagaac ataattcct tagaaacta ttccagccg acacgtttg ctcccatga ataacttgc acacgttaa ttatttcct ataataaaa tcacgtacc tcgacgcat actcgctaa acgggctgt acgttctgt ctctgctgg cgcttgata ataatctcc tcgagggaa ttcatcccg aagctttca gaaactgtt ccagtttgc aggacttga atttgaacg gtaactcac tgaaagccg tgcctctag cactcaaag atatgcgtt tacttcgta cagtggatt taggcgaaa acttgatct ctaacctcg aagagcccg gtttccgag gcatgagca acttgtacg gcttgaggt taatcggta atcaacttc agaacatca gtaaacgcg catttaacg atttaccag cactacaaa ttttaaatc tcgcccgca ataaaatcc agagaatag agcacggag ctttcgaat cgagtccca acctccagg cgatacgtc tcgacgaca acgttttaa cggacgtta gcggtcttt tcgccaata ttcccagtt tattatggc taaacatat ccgataacc aaatcgaat ggttcgatt acgccctca tccccaaag gactgcaat ggctcgatg tccacaaaa acaaagtcc gggaacttg ccaatcgca acaacttgg aactacaat tacgcttgc agactttag acgccagtt ttaacaaac taacgagga ttaatgccg cggccgtgc ccaacagca tcgagttat tatttctaa acgacaatc ttataacca cagtcgaac cacacactt tcctaaaga aaacaaacc tgacgagag tggatttat acgcaaatc agattgtta gtatggatc tgaacgctt tacgcttaa ctccggtcg atccggaaa gaccgttgc ccgaatttt atatcggag gtaatccgt atcagtgcg attgcacca tggagtggc tgcaacgca tcaataaat tggatcatt tgcgccaac accctcgtg tcatggatt tggaaagta tttattgca agcttctgt acaatcgcg atagcaact atctcccgt tgatcgatg ccgaatcgt cgcagttcc tctgcactt acaagacgc attgttttg ctctttgcc attgttgcg actttgacg cctgtgatt gcgaaatga cgtgtccca caaactgta cttgctatc acgatcaat cctggtcgg ccaacgtcg tcgaatgtt ccggtgtgg gctacacag aaatgccta gtcgaatcc ccatggacg ctactgaag tctacttag acggaaaca attttggcg agttgtcga gtcactcgt tcattgggc gaaaaaacc taaagattt tgtacgcca acaattcga acattgctg cgatttaca atcacacgt ttagtggct tgcgccgct taaccatct tgcacttgg agaataaca aaatcaagg agttgttgg ggttcgaat tggcgagtc tggaaaatt tgagggagc tttatttgc aaggaaata tgatccact acattgaca ataggacgt tcctggaat tgaagcaat tggaggtgt tacgtttgg acgggaatc gcttgaatt ctttcgagg tgtggcaat ttgcattga atccatatt tggtcgaaa taggattag gggaaaatc agtggtctt gcgaatgcc actacgtgg agaaattta agtcgtggt ttaaggtaa accacggga aagtgctcg atgctggta aagtagttt gtatgttta acaacgtaa cgaaagctg tgggccccc acatgggcg atttcaacg caaccgctt gtgctgcct acaccgggg gcacccgag ccatcgtgg aaacccaag ttatgaacg actatttgc ctttactcc tcgtcacaa tgtgcgttt tcctggcca gcgttgtga tcgtttgtg gcgcctttt actaccgtc gcgagttac gcgtgtgga tatactctc ggtgcggct tgcgaatgt gctacaaga ccacagctt tcgaagagc agcaagaca aagaccgcc ttttcgacg cctacgtca gctacagcg tcaaggacg aagccttcg tcagccagg tcctggctc cgggcctgg agtccggcg atccgggct accgcttgt gtttgcact accgggact ttaacgtgt ccgcgtacg tcgccgaca cgataatcg aagcggtgg agtcgtccc gacgcacaa ttatcgtcc tttcgaaaa actttctcc agaacgagt ggtgtcgct tcgagttca agtccgccc tgcacgaag tgttgaaag accggaggc gacgcctca ttttcgtgg tcactggcg agttgcccc agcgggatt tggacccgg acttgcggt tgtacttga agacgaaca cagtgatcg agtgggggg accgccagt tctggcaaa aactccagt ttgcgatgc cggacgtgc gaaggccgt gcttgcacc agaggtcgg ccaatattt acgcttcgg cgacgccgt caaggagtc cgtctgcca agagacgct cgcaacatg ccccatcct ctgtgggcg tagcggatg ttcagccgc agcgatcga cagctccag cgtgcgttt ttgacgaat taaattcgg agatggacg gtctttcct tggctcccg aggtgactg tgattgtga tatttttgt gaactttaa ttattctaa atagatggc ccattcgtg tgaacgggc gcgtttcta gttaattat tgttgttat aatttataa acaaaggac tgaaaaagt ttgaatcga atttaacta aaaaagtta taaagtcag tcttgatat atcaatgtt ctttacata ctgcttcat gagcttatt attaatagg tgtattgaa agaaattct catgtatta taaatattt caattagtt caaacagta ctgtgacgt ttcatttat cgatcagct agtaaggtt tttctaact ttaaacggg ttttacgtt gttttatac gccgattat tattattta tttgccgcg tattacaga aaggttgtg atttgtgta tttcctaca gaacttgtt attagtatt ttaacagta tccacaacg atattgata gacttaagt atcttgatt ggacaagtc gacatttga tcgttccgt tttggtcaa acaccaatt tttacaaaa tcaataagt catcaccaa attattttt agttttgat gccactgga aacgaagcg atcaagtgg aggacatcc atgatttac gattcctaa cttaatata ttgtaatat tattaattg tatatactg tgtataaat aatttaaag gtatatttt catcagaaa tcccaaagc acactatgt aaatagcat tattatttg cttatttgg aacgtaact taaaacaaa taaaattat ttaatttta
