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Gene information
General information
TC004879 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1537076..1538345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004879
    iBeetle screen iB_03866
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004879 RA
      MTSNVPNIT TNNGVNIPM LGLGTWKSK PGEVTQAVK 
      DAIDVGYRH IDCAHVYCN EKEVGEALK AKIAEGVIK 
      REDIFITSK LWNTYHRPD LVEKALRTT LANLGLEYL 
      DLYLIHWPF ALKEGDELF PVDEAGKAV YSDVDYVDT 
      WKAMEEMSK KGLTRLIGI SNFNKRQIE RLLQNATIV 
      PATNQIECH PYLTQLKLS DFCKSKGIT ITAYSPLGS 
      PDRPWAKPG DPLLLEDEK IKKIAEKYN KTPAQVVLK 
      YQIQRGHIV IPKSVTQSR IRENFNIWD FELGADDIE 
      LINSFDCNG RICPYVDAV THKDHPFAN DEY
    CDS sequence
    • >TC004879 RA
      atgacgagt aatgtacca aatattacc accaacaat 
      ggggtgaac atccccatg ctggggtta gggacttgg 
      aagtccaaa cctggggag gtaacccaa gcggtgaaa 
      gacgcaatt gacgtcggt tatcgccac attgattgt 
      gcccacgtt tattgcaac gaaaaggaa gtcggggaa 
      gcactgaag gcaaaaatc gccgaaggt gtgatcaaa 
      cgtgaggat attttcatc accagcaaa ctgtggaac 
      acgtaccat cggccagat ttggtcgag aaagccctc 
      agaacaaca cttgctaat ttaggactg gaatacctc 
      gacctttac ctgatccac tggcctttt gccttgaaa 
      gaaggcgac gagttgttt ccggtggac gaggcagga 
      aaggcggtt tatagcgat gttgattac gtcgacacg 
      tggaaagct atggaagaa atgagcaaa aagggccta 
      actaggtta atcggaatc tcaaacttc aacaagcgg 
      caaatcgag agattgcta caaaacgcc acaattgtt 
      ccagcaact aatcaaatc gagtgtcac ccttatttg 
      actcaactg aaactctcc gatttttgc aaatcgaag 
      ggtattacc atcacagcg tacagtccc ttgggatcc 
      cccgaccga ccgtgggct aaaccgggg gatcccctt 
      ttacttgaa gatgagaaa attaagaaa attgcagag 
      aaatataac aaaacgcca gctcaagtc gtgttaaaa 
      taccaaatt cagagggga catatcgtg attcccaaa 
      tctgtaaca caatcaagg attcgggaa aatttcaat 
      atttgggat tttgaactt ggggccgat gatatcgaa 
      ttgattaac tcgtttgac tgcaatgga cgaatctgt 
      ccttatgtg gatgctgtg acacacaag gaccatcct 
      tttgccaac gatgaatat taa
    mRNA sequence
    • >TC004879 RA
      tcgcattat tattctaaa atgacgagt aatgtacca 
      aatattacc accaacaat ggggtgaac atccccatg 
      ctggggtta gggacttgg aagtccaaa cctggggag 
      gtaacccaa gcggtgaaa gacgcaatt gacgtcggt 
      tatcgccac attgattgt gcccacgtt tattgcaac 
      gaaaaggaa gtcggggaa gcactgaag gcaaaaatc 
      gccgaaggt gtgatcaaa cgtgaggat attttcatc 
      accagcaaa ctgtggaac acgtaccat cggccagat 
      ttggtcgag aaagccctc agaacaaca cttgctaat 
      ttaggactg gaatacctc gacctttac ctgatccac 
      tggcctttt gccttgaaa gaaggcgac gagttgttt 
      ccggtggac gaggcagga aaggcggtt tatagcgat 
      gttgattac gtcgacacg tggaaagct atggaagaa 
      atgagcaaa aagggccta actaggtta atcggaatc 
      tcaaacttc aacaagcgg caaatcgag agattgcta 
      caaaacgcc acaattgtt ccagcaact aatcaaatc 
      gagtgtcac ccttatttg actcaactg aaactctcc 
      gatttttgc aaatcgaag ggtattacc atcacagcg 
      tacagtccc ttgggatcc cccgaccga ccgtgggct 
      aaaccgggg gatcccctt ttacttgaa gatgagaaa 
      attaagaaa attgcagag aaatataac aaaacgcca 
      gctcaagtc gtgttaaaa taccaaatt cagagggga 
      catatcgtg attcccaaa tctgtaaca caatcaagg 
      attcgggaa aatttcaat atttgggat tttgaactt 
      ggggccgat gatatcgaa ttgattaac tcgtttgac 
      tgcaatgga cgaatctgt ccttatgtg gatgctgtg 
      acacacaag gaccatcct tttgccaac gatgaatat 
      taaatttaa aaaacatcg gattttgtt tgtttagta 
      aaccttctg tgcacattt taaaaaata atgagaatt 
      ttgaaataa atttgtatt tattac