Status
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Gene information
General information
TC004859 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3263199..3278375
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004859
    iBeetle screen iB_03861

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bent - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004859 RA
      MAARRLPSS APMESVPNS CDPLRELFE ACKVGDISR 
      VRKLVTPQT VNARDTAGR KSTPLHFAA GYGRRDVVE 
      FLLSAGASI QARDDGGLH PLHNACSFG HADVVRLLL 
      EAGANPNTR DNWNYTPLH EAAIKGKVD VCIALLQHG 
      AEPDITNSE GKTPLDVAD NSTRAVLTG EYRKDELLE 
      AARCGAEDK LLALLTPLN VNCHASDGR RSTPLHLAA 
      GYNRSRVVQ LLLQHGADV HAKDKGGLV PLHNACSYG 
      HFEVTEMLI KHGANVNAN DLWAFTPLH EAASKSRLE 
      VCSLLLSEG ADPTQLNCH SKSAIDVAP TRELQERLS 
      CEYRGHQLL DACKQADTT KLKKFLTSE VVNFKHPYT 
      GDTALHVAV NSPYPKRKQ IIELLIRKG IHLNEKNKD 
      FLTPLHLAA DNSHLDLME VLLRHGAKV NALDGLGQT 
      ALHRCTKDD NVQACRLLL SYNVDPSIV SLQGYTAAQ 
      LAGENAQKL FQDPPAVNG DVECQVLEA AKSGDLEQV 
      QRLLETYPN TVNCRDLDG RHSTPLHFA SGYNRVAVV 
      EYLLEQGAD VHAKDKGGL VPLHNACSY GHYEVTELL 
      VKHGANVNV ADLWKFTPL HEASAKGKY EIVKLLLKH 
      GADPTKKNR DGATALDLV REGDQDVAD LLRGNAALL 
      DAAKKGNLA RIQRLITAE NINCRDVQG RNSTPLHLA 
      AGYNNVEVA EYLLEHGAD VNAQDKGGL IPLHNASSY 
      GHLDIAALL IKYNTVVNA TDKWGFTPL HEAAQKGRT 
      QLCALLLAH GADPFLKNQ EGQTPMDLS SAEDVRCLL 
      QDAMASQQG VPTAPPSRP VSVVSSPSP SLTPTVNTE 
      TVLMPSGAS IQLSIPIGS RSSVAIVEG CCSSSKLEG 
      DDQDLIGNI TNIPSFLAS LNLEQLIDI FEREQITLD 
      ILAEMNHDD LKQIGISAY GFRHKLIKG MERLMSSCE 
      GLRLMPSNP GTLLIDLLA DDKEFITVE EEMQNSIRE 
      HRDQGHAGG VFNRYNIVR IQKVQNRKL WERYTHRRQ 
      EVSEENNNQ CNERMLFHG SPFINAIVQ KGFDERHAY 
      IGGMFGAGI YFGEHSSKS NQYVYGIGG GTGCPTHKD 
      RSCYICHRH LLLCRVTLG KMFLQFSAM KMAHAPPGH 
      HSVAGRPSA GGLNFPEYV VYRGEQAYP EYLITYQIV 
      KPENCPMTS DSGVR
    CDS sequence
    • >TC004859 RA
      atggcggcc cgtcgtctc ccgagttcg gccccaatg 
      gagtccgtc cccaattcc tgcgatcct cttcgcgag 
      ctgttcgag gcttgcaaa gtcggggac atctcccgc 
      gtccgcaaa ctggtaacc ccccaaaca gtcaacgct 
      cgcgataca gctggccgc aaatctact ccgcttcat 
      ttcgcagct ggatacggc agacgggac gtggtggag 
      ttcctgtta tcggccggg gcttccatc caggctcgc 
      gacgacggg ggcctccac cccttgcac aacgcgtgt 
      tcctttgga catgcggac gttgtgcgg ttgcttctg 
      gaggcaggg gcgaatcca aacacgcgc gataactgg 
      aattacacg ccgctgcat gaggctgcg attaaggga 
      aaggtcgat gtttgtatt gctctgctg cagcacggg 
      gctgaaccc gatataacg aattcggaa gggaaaact 
      ccgttagat gttgctgat aattcaact agagctgtt 
      ttgacgggg gagtaccgc aaagatgag cttctggaa 
      gctgcacgt tgcggtgct gaagacaag cttttagcg 
      cttttgact ccacttaat gttaattgt catgctagt 
      gatggacgg cgctcgacg cctctgcat ttggcagct 
      gggtataat cggagtcgt gtggtgcag ttgttgcta 
      caacatgga gcagatgtc catgccaaa gataaaggc 
      ggcctcgtc cctctacac aacgcgtgt tcatacggc 
      cacttcgaa gtgaccgaa atgctaatt aaacatggt 
      gctaacgtc aacgccaac gatctctgg gcttttaca 
      cccttacac gaagcggct tcaaaatca cgccttgaa 
      gtgtgttcg ttattactg agcgaaggt gcggatcca 
      actcagctc aattgtcat tcgaaatcg gcaatcgat 
      gtagcaccg actcgagaa ttacaagaa cgactctca 
      tgtgagtat cgtggacat caattactg gatgcttgc 
      aaacaagcc gacaccacc aaactaaaa aagttcctc 
      acatccgaa gtagtcaat tttaaacat ccatatacc 
      ggtgatacg gcgctacat gtagccgtg aattcgccc 
      tatccgaag cgtaaacaa ataatcgaa ttgttgatt 
      cgtaaagga attcatttg aatgagaaa aataaagat 
      tttctgaca ccgcttcat ctggctgct gataattcg 
      catcttgat ctcatggag gtgctacta agacacggt 
      gctaaagtc aatgctttg gacggatta ggacaaact 
      gccttgcac agatgtacc aaagatgat aacgtccaa 
      gcttgccga cttttactg tcctacaac gtcgatccc 
      tcaattgtc tcgctacag ggctacaca gcggcgcaa 
      ttagccggc gaaaacgcc caaaaactg ttccaagat 
      cctccagcc gttaacggc gatgtcgaa tgccaagtg 
      ttggaagct gcgaaatcc ggcgatctt gaacaggtt 
      caaagacta ctagaaacg taccccaac acggttaac 
      tgtcgcgat ttggacggt cgtcattcg actcccctc 
      catttcgcg tccggttat aaccgagta gccgttgtc 
      gagtatctc ctcgagcaa ggagccgac gtacatgcg 
      aaagataaa ggcggttta gtgccttta cacaacgct 
      tgctcctac ggtcactac gaagtgacc gaattatta 
      gtcaaacat ggagctaat gtaaacgtt gccgatttg 
      tggaagttt acaccgtta cacgaggct tcagcgaaa 
      ggtaaatac gagattgtg aaattgttg ttgaaacat 
      ggtgctgat cccacgaag aaaaaccga gatggagct 
      acggctctt gatttggtg cgagagggt gatcaagat 
      gttgccgat ttgttgcga gggaatgca gcattgttg 
      gatgccgcc aaaaagggg aatttggcg aggattcaa 
      cggttgatt accgcggaa aatatcaat tgtcgcgat 
      gtgcaaggg agaaattcc actccgctc catttggcc 
      gcgggctat aataatgtg gaagttgcc gagtattta 
      ctcgaacat ggggctgat gttaacgct caggataag 
      ggtggttta ataccgttg cataacgct tccagttac 
      ggtcattta gatattgct gctttgttg attaagtat 
      aatactgtg gtgaatgct acggacaag tggggtttt 
      acgccgttg catgaagcc gcccagaaa ggcaggact 
      caactgtgt gcccttctt ttagcgcat ggcgctgac 
      ccatttctg aaaaaccaa gaaggccaa actccgatg 
      gatttatca agcgctgaa gacgtccgt tgtctcctt 
      caagacgca atggcgtcg caacaaggt gtgccaaca 
      gcgccccct tcacgtcca gtctccgtc gtttcttcg 
      ccaagtcca tctctcact cctactgtc aataccgag 
      actgtttta atgccatcg ggcgcttca attcaatta 
      tcgattcct ataggtagt agatcaagt gtggcaata 
      gttgagggt tgttgttcc tcctcgaaa ttggagggt 
      gacgatcag gatttaatc ggaaatatt acgaatatt 
      ccttcgttc cttgctagt ctcaatttg gaacaattg 
      attgatatt ttcgaaaga gagcaaatc acgttggat 
      attttggcc gaaatgaat cacgacgat ttgaaacaa 
      attgggatt tcggcgtat ggttttcga cacaagttg 
      atcaaagga atggaacgg ttgatgtcg agttgtgaa 
      ggcttgagg ttgatgccg tcgaatccg gggactttg 
      cttattgac cttttggca gacgataaa gagtttatt 
      actgttgaa gaggagatg cagaatagt atcagggag 
      catagggat caagggcat gcgggcggt gtcttcaat 
      cgatacaac atcgtcagg atacagaaa gtccaaaac 
      cggaagctg tgggagcgc tacacccac cgtcgccag 
      gaagtgtcg gaagagaac aacaaccaa tgcaacgaa 
      aggatgttg tttcacggt tcacccttt attaacgcg 
      attgtgcaa aagggtttc gatgaaagg catgcgtat 
      atcgggggg atgttcggg gctgggatt tacttcggg 
      gagcattcg tctaaaagc aaccaatac gtgtatggg 
      atcgggggc ggcaccgga tgtccgaca cacaaagac 
      cgctcttgt tatatttgt cacaggcat cttttgctc 
      tgtcgcgtg actttagga aagatgttt cttcagttt 
      agcgccatg aagatggca catgcccct ccaggacac 
      cattcagtg gccggaagg ccgtctgct ggtggttta 
      aatttcccg gaatatgtg gtttacaga ggggaacag 
      gcttatccc gagtacctc atcacgtac cagattgtt 
      aaacctgag aattgtcca atgacgagt gatagtggt 
      gtcaggtga
    mRNA sequence
    • >TC004859 RA
      ttgcgtgaa aaatttgac agttgttga cgagggtgt 
      tttatggta gtgtttgta gcaataata atcaatatt 
      tttgcaatc ttaaatgtg aaatgagtg gttccgcta 
      gctgattcc gactttgga atggcggcc cgtcgtctc 
      ccgagttcg gccccaatg gagtccgtc cccaattcc 
      tgcgatcct cttcgcgag ctgttcgag gcttgcaaa 
      gtcggggac atctcccgc gtccgcaaa ctggtaacc 
      ccccaaaca gtcaacgct cgcgataca gctggccgc 
      aaatctact ccgcttcat ttcgcagct ggatacggc 
      agacgggac gtggtggag ttcctgtta tcggccggg 
      gcttccatc caggctcgc gacgacggg ggcctccac 
      cccttgcac aacgcgtgt tcctttgga catgcggac 
      gttgtgcgg ttgcttctg gaggcaggg gcgaatcca 
      aacacgcgc gataactgg aattacacg ccgctgcat 
      gaggctgcg attaaggga aaggtcgat gtttgtatt 
      gctctgctg cagcacggg gctgaaccc gatataacg 
      aattcggaa gggaaaact ccgttagat gttgctgat 
      aattcaact agagctgtt ttgacgggg gagtaccgc 
      aaagatgag cttctggaa gctgcacgt tgcggtgct 
      gaagacaag cttttagcg cttttgact ccacttaat 
      gttaattgt catgctagt gatggacgg cgctcgacg 
      cctctgcat ttggcagct gggtataat cggagtcgt 
      gtggtgcag ttgttgcta caacatgga gcagatgtc 
      catgccaaa gataaaggc ggcctcgtc cctctacac 
      aacgcgtgt tcatacggc cacttcgaa gtgaccgaa 
      atgctaatt aaacatggt gctaacgtc aacgccaac 
      gatctctgg gcttttaca cccttacac gaagcggct 
      tcaaaatca cgccttgaa gtgtgttcg ttattactg 
      agcgaaggt gcggatcca actcagctc aattgtcat 
      tcgaaatcg gcaatcgat gtagcaccg actcgagaa 
      ttacaagaa cgactctca tgtgagtat cgtggacat 
      caattactg gatgcttgc aaacaagcc gacaccacc 
      aaactaaaa aagttcctc acatccgaa gtagtcaat 
      tttaaacat ccatatacc ggtgatacg gcgctacat 
      gtagccgtg aattcgccc tatccgaag cgtaaacaa 
      ataatcgaa ttgttgatt cgtaaagga attcatttg 
      aatgagaaa aataaagat tttctgaca ccgcttcat 
      ctggctgct gataattcg catcttgat ctcatggag 
      gtgctacta agacacggt gctaaagtc aatgctttg 
      gacggatta ggacaaact gccttgcac agatgtacc 
      aaagatgat aacgtccaa gcttgccga cttttactg 
      tcctacaac gtcgatccc tcaattgtc tcgctacag 
      ggctacaca gcggcgcaa ttagccggc gaaaacgcc 
      caaaaactg ttccaagat cctccagcc gttaacggc 
      gatgtcgaa tgccaagtg ttggaagct gcgaaatcc 
      ggcgatctt gaacaggtt caaagacta ctagaaacg 
      taccccaac acggttaac tgtcgcgat ttggacggt 
      cgtcattcg actcccctc catttcgcg tccggttat 
      aaccgagta gccgttgtc gagtatctc ctcgagcaa 
      ggagccgac gtacatgcg aaagataaa ggcggttta 
      gtgccttta cacaacgct tgctcctac ggtcactac 
      gaagtgacc gaattatta gtcaaacat ggagctaat 
      gtaaacgtt gccgatttg tggaagttt acaccgtta 
      cacgaggct tcagcgaaa ggtaaatac gagattgtg 
      aaattgttg ttgaaacat ggtgctgat cccacgaag 
      aaaaaccga gatggagct acggctctt gatttggtg 
      cgagagggt gatcaagat gttgccgat ttgttgcga 
      gggaatgca gcattgttg gatgccgcc aaaaagggg 
      aatttggcg aggattcaa cggttgatt accgcggaa 
      aatatcaat tgtcgcgat gtgcaaggg agaaattcc 
      actccgctc catttggcc gcgggctat aataatgtg 
      gaagttgcc gagtattta ctcgaacat ggggctgat 
      gttaacgct caggataag ggtggttta ataccgttg 
      cataacgct tccagttac ggtcattta gatattgct 
      gctttgttg attaagtat aatactgtg gtgaatgct 
      acggacaag tggggtttt acgccgttg catgaagcc 
      gcccagaaa ggcaggact caactgtgt gcccttctt 
      ttagcgcat ggcgctgac ccatttctg aaaaaccaa 
      gaaggccaa actccgatg gatttatca agcgctgaa 
      gacgtccgt tgtctcctt caagacgca atggcgtcg 
      caacaaggt gtgccaaca gcgccccct tcacgtcca 
      gtctccgtc gtttcttcg ccaagtcca tctctcact 
      cctactgtc aataccgag actgtttta atgccatcg 
      ggcgcttca attcaatta tcgattcct ataggtagt 
      agatcaagt gtggcaata gttgagggt tgttgttcc 
      tcctcgaaa ttggagggt gacgatcag gatttaatc 
      ggaaatatt acgaatatt ccttcgttc cttgctagt 
      ctcaatttg gaacaattg attgatatt ttcgaaaga 
      gagcaaatc acgttggat attttggcc gaaatgaat 
      cacgacgat ttgaaacaa attgggatt tcggcgtat 
      ggttttcga cacaagttg atcaaagga atggaacgg 
      ttgatgtcg agttgtgaa ggcttgagg ttgatgccg 
      tcgaatccg gggactttg cttattgac cttttggca 
      gacgataaa gagtttatt actgttgaa gaggagatg 
      cagaatagt atcagggag catagggat caagggcat 
      gcgggcggt gtcttcaat cgatacaac atcgtcagg 
      atacagaaa gtccaaaac cggaagctg tgggagcgc 
      tacacccac cgtcgccag gaagtgtcg gaagagaac 
      aacaaccaa tgcaacgaa aggatgttg tttcacggt 
      tcacccttt attaacgcg attgtgcaa aagggtttc 
      gatgaaagg catgcgtat atcgggggg atgttcggg 
      gctgggatt tacttcggg gagcattcg tctaaaagc 
      aaccaatac gtgtatggg atcgggggc ggcaccgga 
      tgtccgaca cacaaagac cgctcttgt tatatttgt 
      cacaggcat cttttgctc tgtcgcgtg actttagga 
      aagatgttt cttcagttt agcgccatg aagatggca 
      catgcccct ccaggacac cattcagtg gccggaagg 
      ccgtctgct ggtggttta aatttcccg gaatatgtg 
      gtttacaga ggggaacag gcttatccc gagtacctc 
      atcacgtac cagattgtt aaacctgag aattgtcca 
      atgacgagt gatagtggt gtcaggtga tttggttgc 
      aaggataca gtgaaggag ttgtggtac ggagtcgtg 
      gctattttg ctcaaatgt ttcgttcca acaacgatt 
      tgtacttgt caaaagtcg aggcaataa tgatgccct 
      tttatgtgt tcataattc atgttttac tgatgtaaa 
      gcgaggact gaatctgca gcaaattgt gatatctca 
      tttcgaacg attagtcaa agcgatttt tgttttatt 
      tatggtgtg gtgtttgtc tttattttt actgtagtg 
      ttaataaaa tatacacaa acat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY