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Gene information
General information
TC004850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3058499..3069564
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004850
    iBeetle screen iB_03858

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004850 RA
      MLKLVAFIL YCTNLANGQ TTQNINVLF VNEEGNLVA 
      EKAVDVATN YIKKNNKLG VNADPVKVV GNRTDASGL 
      LDSLCSSYN EMIANSMNP HLVLDTTMT GLASETVKS 
      FTAALGLPT ISASFGQEG DLRQWRNID ENEKEYLVQ 
      ISPPADVIP EIIRSLVLS KNVTNAAIL FDDSFVMDH 
      KYKSLLQNV ATRHVIAPI KEADKIGDQ LRQLRKLDI 
      VNFFILGSF ENIKRVLDA ADSVGFFNR KFSWHAITQ 
      DKGELKCNC RNATITLAK PLIDAQYQD RLGLIKTSY 
      QLNAEPEIA AAFYFDLAL YSFLAVKEM IADGVWKRN 
      NATNYITCD DFDGKNTPR RAGLNLKKY FSKEVSETP 
      TYGPISIVS NGYSFMEFT MQISAVGVR ESSSDKSVP 
      LGSWKAGYD NNLTLVDPQ IMKNYTADV VYRVVTVEQ 
      KPFIIKDET APKGYKGYC IDLIQRISE ILNFDYEIT 
      PVGDQKFGN MDENGKWNG VVRELMEKR ADIGLGSMS 
      VMAERENVI DFTVPYYDL VGITILMKL PKTPTSLFK 
      FLTVLENEV WLCILAAYF FTSFLMWVF DRWSPYSYQ 
      NNREKYKDD EEKREFNLK ECLWFCMTS LTPQGGGEA 
      PKNLSGRLV AATWWLFGF IIIASYTAN LAAFLTVSR 
      LDTPIESLD DLSKQYKIQ YAPLNGSST MTYFERMAN 
      IEAKFYEIW KDMSLNDSL SEVERAKLA VWDYPVSDK 
      YTKMWQAMK EAGLPNTLD EAVKRVKDS RSSSEGFAY 
      LGDATDIRY LEITSCDLQ MVGEEFSRK PYAIAVQQG 
      SPLKDQFNT AILQLLNRR ELERLKEKW WSKNPEAKK 
      CDKQEDQSD GISIQNIGG VFIVIFVGI GLACITLAF 
      EYWWYKYRK GGKVVDVQA KHSDVATKI NDGFHAKIN 
      KLYPRSRF
    CDS sequence
    • >TC004850 RA
      atgctcaaa ctcgtcgca ttcatcctg tattgcaca 
      aatttggca aacgggcaa accacgcaa aacatcaac 
      gttttattt gtgaacgag gaaggcaat ttagttgcg 
      gaaaaagcc gtcgatgtt gccaccaat tacataaag 
      aaaaacaac aagctgggg gtcaatgcc gaccctgtc 
      aaggtcgtg ggcaacagg actgatgcc tcgggactc 
      ctagattca ttgtgtagc agttacaat gaaatgatt 
      gcaaactcg atgaaccca catttggtc ctcgacaca 
      accatgaca ggactcgcc tcagaaacg gtaaaatcg 
      ttcaccgca gccttgggt ttgccaacg ataagcgcg 
      agctttggc caggaaggc gatttgcgc cagtggcgc 
      aatatcgac gaaaatgag aaggaatat ctcgtgcaa 
      atttcacca ccagctgac gtaatcccg gaaataatc 
      cgctcctta gtccttagc aaaaatgtg acaaacgct 
      gcgatcctc ttcgatgat tctttcgtc atggaccac 
      aagtacaag tctctgctc caaaacgtg gccacgcga 
      cacgtcatt gccccgatt aaagaagct gacaaaatc 
      ggcgatcag ttgcgacag ctccgcaaa ctcgacatt 
      gtcaatttc ttcattttg ggcagtttt gaaaacatc 
      aaacgtgtc ttggacgct gcggacagt gtcggtttc 
      ttcaaccgg aagttctcc tggcatgcc atcacacaa 
      gacaagggc gaactgaaa tgtaactgc agaaacgca 
      acgattacg ttggccaag cctttgatc gacgcccag 
      tatcaggat cgtctcgga cttatcaaa acctcttat 
      cagctgaat gccgaaccg gagatcgca gctgccttc 
      tacttcgac ctagccctt tactcattc ctcgcagtc 
      aaggaaatg atcgctgat ggtgtttgg aagcgaaac 
      aacgcgaca aactacata acttgcgac gactttgac 
      gggaaaaac accccgagg cgtgcgggc cttaacttg 
      aaaaaatac ttcagcaag gaagtttcc gaaacccca 
      acttatggc cccattagc attgtttca aacggttac 
      agtttcatg gagtttacc atgcagata agtgcggtg 
      ggagtgcgc gaaagctcg tcggataag tcggtacct 
      ttgggctct tggaaggcg ggttatgat aacaattta 
      acgcttgtg gatccgcaa attatgaaa aattatacg 
      gccgatgtg gtctaccgt gttgtcacg gttgagcaa 
      aaaccgttc attattaag gacgaaacg gcccccaaa 
      ggctacaag ggctattgc attgatcta atacaaaga 
      ataagtgag atattgaac tttgattat gaaattact 
      ccggttggt gaccagaaa ttcggaaat atggacgag 
      aatggcaag tggaatggc gtagttcga gaattgatg 
      gagaaaagg gctgatatt gggctcggg tcaatgtca 
      gtcatggcc gaaagggaa aacgtgatt gatttcacg 
      gtcccgtat tatgatctg gtcggaatt acgattttg 
      atgaagctg ccaaagact ccaacttcg cttttcaag 
      ttcttgacg gtgctcgag aacgaggtt tggttgtgc 
      attttggcg gcctatttc ttcaccagt tttttgatg 
      tgggtcttc gaccggtgg agcccttac agctaccag 
      aacaaccgc gaaaagtac aaggacgac gaggaaaag 
      cgcgaattt aacctgaag gaatgtttg tggttttgt 
      atgacatca ctgacacca caaggcgga ggcgaagcg 
      ccgaaaaat ttatctggt cggttggtt gcagccact 
      tggtggctc ttcggtttc attatcatt gcctcatac 
      actgcgaat cttgcggct ttcttgacc gtttcgcgc 
      ttggatact cccatcgag tccttggac gacttatcc 
      aaacagtac aaaattcaa tatgcaccg cttaacgga 
      tcgtccact atgacttac ttcgaacga atggcaaac 
      attgaggcg aagttttac gagatttgg aaggatatg 
      agcttgaat gatagcctt tcggaagtg gagagggcg 
      aaattagca gtttgggat tatccagtc agtgataaa 
      tacaccaag atgtggcaa gctatgaag gaagctggt 
      ttaccaaac actctggac gaagctgtg aaacgggtg 
      aaggattcc cggtcttca agcgaaggt tttgcctat 
      ttgggtgat gccactgat atcagatat ctcgaaatc 
      acaagttgt gacctgcaa atggtcggc gaggaattt 
      tccagaaaa ccgtacgca atcgccgtt caacaagga 
      tcacccttg aaggaccaa ttcaatacg gcaattttg 
      caattactc aatcgccgc gaactcgaa cgactgaag 
      gagaaatgg tggagcaaa aacccggaa gccaagaaa 
      tgtgacaaa caagaggac cagtccgac ggaatcagc 
      attcagaac attgggggc gttttcatt gtcattttc 
      gtaggaatt ggactggct tgtatcaca ctagcgttt 
      gagtactgg tggtataaa tacaggaaa ggcgggaaa 
      gtggtggac gtccaagcc aaacattcc gatgtggcg 
      acgaaaatt aacgacggg tttcatgca aaaataaac 
      aagttgtat ccacgatca cgattttaa
    mRNA sequence
    • >TC004850 RA
      atcgatttc gctgccaaa caacaaacg attaacgaa 
      tcgatacat ttaaacacc attaaagtc ggaattaca 
      caacaagcg tacaagaaa aactaatat caaaactct 
      tccaatatt cctgaaacg gaattggac acgcaagtg 
      aatgtcaag aactgaccc aaaataaaa taatattta 
      cgtcgttca caaaccatt gtccggcaa tagaaaacc 
      taaattatt aatcatgtg tatgtttac accccaatc 
      acttggcaa caaactata acaagcttt ttttcgagc 
      aaagctcgt tcgattaaa cgcatttca caaagcttc 
      ctcaataat gtattaact gaaacgcac aaaatgctc 
      aaactcgtc gcattcatc ctgtattgc acaaatttg 
      gcaaacggg caaaccacg caaaacatc aacgtttta 
      tttgtgaac gaggaaggc aatttagtt gcggaaaaa 
      gccgtcgat gttgccacc aattacata aagaaaaac 
      aacaagctg ggggtcaat gccgaccct gtcaaggtc 
      gtgggcaac aggactgat gcctcggga ctcctagat 
      tcattgtgt agcagttac aatgaaatg attgcaaac 
      tcgatgaac ccacatttg gtcctcgac acaaccatg 
      acaggactc gcctcagaa acggtaaaa tcgttcacc 
      gcagccttg ggtttgcca acgataagc gcgagcttt 
      ggccaggaa ggcgatttg cgccagtgg cgcaatatc 
      gacgaaaat gagaaggaa tatctcgtg caaatttca 
      ccaccagct gacgtaatc ccggaaata atccgctcc 
      ttagtcctt agcaaaaat gtgacaaac gctgcgatc 
      ctcttcgat gattctttc gtcatggac cacaagtac 
      aagtctctg ctccaaaac gtggccacg cgacacgtc 
      attgccccg attaaagaa gctgacaaa atcggcgat 
      cagttgcga cagctccgc aaactcgac attgtcaat 
      ttcttcatt ttgggcagt tttgaaaac atcaaacgt 
      gtcttggac gctgcggac agtgtcggt ttcttcaac 
      cggaagttc tcctggcat gccatcaca caagacaag 
      ggcgaactg aaatgtaac tgcagaaac gcaacgatt 
      acgttggcc aagcctttg atcgacgcc cagtatcag 
      gatcgtctc ggacttatc aaaacctct tatcagctg 
      aatgccgaa ccggagatc gcagctgcc ttctacttc 
      gacctagcc ctttactca ttcctcgca gtcaaggaa 
      atgatcgct gatggtgtt tggaagcga aacaacgcg 
      acaaactac ataacttgc gacgacttt gacgggaaa 
      aacaccccg aggcgtgcg ggccttaac ttgaaaaaa 
      tacttcagc aaggaagtt tccgaaacc ccaacttat 
      ggccccatt agcattgtt tcaaacggt tacagtttc 
      atggagttt accatgcag ataagtgcg gtgggagtg 
      cgcgaaagc tcgtcggat aagtcggta cctttgggc 
      tcttggaag gcgggttat gataacaat ttaacgctt 
      gtggatccg caaattatg aaaaattat acggccgat 
      gtggtctac cgtgttgtc acggttgag caaaaaccg 
      ttcattatt aaggacgaa acggccccc aaaggctac 
      aagggctat tgcattgat ctaatacaa agaataagt 
      gagatattg aactttgat tatgaaatt actccggtt 
      ggtgaccag aaattcgga aatatggac gagaatggc 
      aagtggaat ggcgtagtt cgagaattg atggagaaa 
      agggctgat attgggctc gggtcaatg tcagtcatg 
      gccgaaagg gaaaacgtg attgatttc acggtcccg 
      tattatgat ctggtcgga attacgatt ttgatgaag 
      ctgccaaag actccaact tcgcttttc aagttcttg 
      acggtgctc gagaacgag gtttggttg tgcattttg 
      gcggcctat ttcttcacc agttttttg atgtgggtc 
      ttcgaccgg tggagccct tacagctac cagaacaac 
      cgcgaaaag tacaaggac gacgaggaa aagcgcgaa 
      tttaacctg aaggaatgt ttgtggttt tgtatgaca 
      tcactgaca ccacaaggc ggaggcgaa gcgccgaaa 
      aatttatct ggtcggttg gttgcagcc acttggtgg 
      ctcttcggt ttcattatc attgcctca tacactgcg 
      aatcttgcg gctttcttg accgtttcg cgcttggat 
      actcccatc gagtccttg gacgactta tccaaacag 
      tacaaaatt caatatgca ccgcttaac ggatcgtcc 
      actatgact tacttcgaa cgaatggca aacattgag 
      gcgaagttt tacgagatt tggaaggat atgagcttg 
      aatgatagc ctttcggaa gtggagagg gcgaaatta 
      gcagtttgg gattatcca gtcagtgat aaatacacc 
      aagatgtgg caagctatg aaggaagct ggtttacca 
      aacactctg gacgaagct gtgaaacgg gtgaaggat 
      tcccggtct tcaagcgaa ggttttgcc tatttgggt 
      gatgccact gatatcaga tatctcgaa atcacaagt 
      tgtgacctg caaatggtc ggcgaggaa ttttccaga 
      aaaccgtac gcaatcgcc gttcaacaa ggatcaccc 
      ttgaaggac caattcaat acggcaatt ttgcaatta 
      ctcaatcgc cgcgaactc gaacgactg aaggagaaa 
      tggtggagc aaaaacccg gaagccaag aaatgtgac 
      aaacaagag gaccagtcc gacggaatc agcattcag 
      aacattggg ggcgttttc attgtcatt ttcgtagga 
      attggactg gcttgtatc acactagcg tttgagtac 
      tggtggtat aaatacagg aaaggcggg aaagtggtg 
      gacgtccaa gccaaacat tccgatgtg gcgacgaaa 
      attaacgac gggtttcat gcaaaaata aacaagttg 
      tatccacga tcacgattt taagggttg ttagacaat 
      ttatttagc acgggcttg caatattgt tgtttgcgc 
      acaagtgtg ttgaaataa aataacctt ttccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY