| General information | |||
| TC004846 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2989808..3002123 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004846 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03857 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
| Comment: effect seems to be strongest in the thoracic and first abdominal segments. leg musculature seems not to be ffected at all. |
larval musculature lateral partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC004846 RA
MTNCLFISD FFSVQLFML LFMCIGVLL FVVDFRGWQ RHRVELLPF EEASVGRDD VALKESCAN RRKQRVRGR ACDLGPGGP THRLLHDAA RDVTAPQRR PVLALRSAE AAHSRVRAR TARECCRRR RRGGASAAW HREAAPAPA ASKMIALMT MRRERDETR SDRERDAGA ADEFVDILQ VQQLLLDGG RREPPPAQA ATYPPPPYY LGQGYGQPA ASVDDLVAL WFAGGNTAS GPPASAPNP PATMIMDGL NTLPAQHHH HHHHHHHSA FLLTESAAA AAAAAHHFN VLSFDTCLY KSVASSVDA GGSPAPAAV CTAADEPAV SEAQPGDLN TPVTTSSDI PSFFGPSTV VEPPPITGS LDPEELTLD QQQQQQQQI SPHHSPSGS HIGERATPQ DSALSPSLR EEENTNHST LSMYPTGTS HANNMGKMH HRTVYTSAS SPNMSGNIQ MQSVEGMAH SPSTVNPWL LSSGDKPIY PAMFGLLGQ GSSQSPQQQ YPSATPSPA GTQYEEQLM SMDCGLKQP PSYPSCSAS TSLQLELQQ QQDLVYSRV GQPLVASTA KYQWDSQEY SPQSSSALV VPSTSNLVP KQEPFSAGC SSEIGQQSN SSYGVQLAE YNPSTSKGH EILSQVYQQ SPVPLKLVP VKPRKYPNR PSKTPVHER PYACPVENC DRRFSRSDE LTRHIRIHT GQKPFQCRI CMRSFSRSD HLTTHIRTH TGEKPFSCD VCGRKFARS DEKKRHAKV HLKQRMKKE SKMGQQQQQ QQQPPQQHM QHHMHASHT VTSEDGLSV VTTTL
- >TC004846 RB
MTNCLFISD FFSVQLFML LFMCIGVLL FVVDFRGWQ RHRVELLPF EEASVGRDD VALKESCAN RRKQRVRGR ACDLGPGGP THRLLHDAA RDVTAPQRR PVLALRSAE AAHSRVRAR TARECCRRR RRGGASAAW HREAAPAPA ASKMIALMT MRRERDETR SDRERDAGA ADEFVDILQ VQQLLLDGG RREPPPAQA ATYPPPPYY LGQGYGQPA ASVDDLVAL WFAGGNTAS APCYVTATS RYVCWWWGV IGGVLLAIG SPLACDFQI WNRYSIHKS KVALAPRPV SEKLPCSED GCPSGVTLD TSGAEDASA VRAAGATSP CSVVGPPAS APNPPATMI MDGLNTLPA QHHHHHHHH HHSAFLLTE SAAAAAAAA HHFNVLSFD TCLYKSVAS SVDAGGSPA PAAVCTAAD EPAVSEAQP GDLNTPVTT SSDIPSFFG PSTVVEPPP ITGSLDPEE LTLDQQQQQ QQQISPHHS PSGSHIGER ATPQDSALS PSLREEENT NHSTLSMYP TGTSHANNM GKMHHRTVY TSASSPNMS GNIQMQSVE GMAHSPSTV NPWLLSSGD KPIYPAMFG LLGQGSSQS PQQQYPSAT PSPAGTQYE EQLMSMDCG LKQPPSYPS CSASTSLQL ELQQQQDLV YSRVGQPLV ASTAKYQWD SQEYSPQSS SALVVPSTS NLVPKQEPF SAGCSSEIG QQSNSSYGV QLAEYNPST SKGHEILSQ VYQQSPVPL KLVPVKPRK YPNRPSKTP VHERPYACP VENCDRRFS RSDELTRHI RIHTGQKPF QCRICMRSF SRSDHLTTH IRTHTGEKP FSCDVCGRK FARSDEKKR HAKVHLKQR MKKESKMGQ QQQQQQQPP QQHMQHHMH ASHTVTSED GLSVVTTTL
- >TC004846 RA
atgacgaat tgtttattt atttccgat tttttcagt gtccagttg tttatgtta ttgtttatg tgtataggt gtgttgtta ttcgttgtc gatttccgc ggctggcaa cggcatcgc gtcgaattg ctacctttc gaggaggcg tccgttggt cgtgatgac gtagccctg aaagagtcg tgtgccaac cgacggaag cagcgggtt cgaggccgt gcgtgtgac ctggggccc gggggaccc acgcaccgg ctgctccat gacgccgcg cgtgacgtc accgccccg cagaggcgg ccggtgttg gcgttgagg agcgccgaa gccgctcat tcacgtgtc cgtgctcga acagcgcgc gagtgctgt cgtcgtcgt cgtcgaggt ggtgcaagt gcggcatgg caccgggaa gccgctccg gcgccggcc gcttccaag atgatagct ctgatgacg atgcgcagg gaacgcgac gagacgcgg agtgatcgc gaacgcgac gccggtgcc gccgacgag ttcgtggac atcctgcag gtgcaacag ctgctgttg gacgggggc cgccgcgag ccgccccca gcacaggcc gccacgtac ccgccgccc ccctattac ctaggacag gggtatgga cagccggcg gccagcgtc gacgatctc gtcgcgctt tggttcgcc ggcggaaac actgcttca gggcccccg gcgagcgcc cccaacccg ccggccacc atgatcatg gacggcctg aacacgctg cccgcacag caccaccac caccaccac catcatcac cactccgcc ttcctgctg accgagtcg gcggccgcc gccgccgcc gccgctcac cacttcaac gtgctcagc ttcgacacg tgtctgtac aagagcgtc gcctcctcg gtggacgcc ggcggcagc cccgcgccc gccgccgtc tgcaccgcc gccgacgag cccgccgtc tccgaggcg cagcccggc gacctcaac acccccgtc accacctcc agcgacatt ccctccttc ttcgggccc tccaccgtc gtcgaaccg ccgcccatc acaggctcc cttgacccg gaggagctc actttggac caacaacag cagcaacag cagcagata agtccccac cattcacct tcaggcagt cacatcggc gaacgtgca accccccaa gacagcgct ctgagtccc agccttcgc gaggaagaa aacacgaac cacagcacc cttagcatg tatcccacg ggaacatct cacgcgaac aacatggga aaaatgcat catcgaacc gtttacaca tcagcgtcg agtccaaac atgtcgggg aacatccaa atgcagtcg gttgaaggc atggctcat tctccctcg acggtgaat ccgtggttg ttgtccagc ggggacaag ccgatttat ccggcgatg ttcgggctt ttggggcaa ggaagctcc caatcgccc caacagcag tacccctca gcaaccccc agccctgca gggacgcaa tacgaggaa cagctcatg agcatggac tgcgggctg aaacaacct ccaagttac cccagttgt tcagcttcg acgagtctc cagttagag ttgcaacaa caacaggac ttggtttat tcccgcgtc gggcagccg ctcgtggcg agcaccgcc aagtaccaa tgggactcc caggagtac agtccgcaa agttcaagt gcgttagtg gtgcccagc acttcgaat ttagtaccg aaacaagaa ccgttcagt gcggggtgt tcgagtgaa atcggccaa caatcaaac tcctcgtac ggtgtgcag ctagcagag tacaaccct tcgacgagt aaaggccac gagatcctc tcccaggtg taccagcag tccccagtc ccgctcaag ctcgtcccg gtcaagccc cgcaagtac cccaaccgc cccagcaaa accccggtc catgagcgc ccctacgca tgtcccgtg gagaactgc gaccgccgc ttttcccgc tccgacgag ctcacgcgc cacatccgc atccacacg ggccagaaa cccttccag tgccgcatc tgcatgcgg tccttctcg cggtcggac cacctcacc acgcacatt cgcacccac acgggggaa aagcccttc tcgtgcgac gtctgcggg aggaagttc gcccgcagc gacgagaag aagaggcac gcgaaggtg cacctcaag cagaggatg aagaaggag agcaagatg gggcagcag cagcagcag cagcagcaa cccccgcaa cagcacatg cagcaccac atgcatgcc agtcacacg gtgaccagt gaggacggc ctctcggtg gtgacgacg actctgtga
- >TC004846 RB
atgacgaat tgtttattt atttccgat tttttcagt gtccagttg tttatgtta ttgtttatg tgtataggt gtgttgtta ttcgttgtc gatttccgc ggctggcaa cggcatcgc gtcgaattg ctacctttc gaggaggcg tccgttggt cgtgatgac gtagccctg aaagagtcg tgtgccaac cgacggaag cagcgggtt cgaggccgt gcgtgtgac ctggggccc gggggaccc acgcaccgg ctgctccat gacgccgcg cgtgacgtc accgccccg cagaggcgg ccggtgttg gcgttgagg agcgccgaa gccgctcat tcacgtgtc cgtgctcga acagcgcgc gagtgctgt cgtcgtcgt cgtcgaggt ggtgcaagt gcggcatgg caccgggaa gccgctccg gcgccggcc gcttccaag atgatagct ctgatgacg atgcgcagg gaacgcgac gagacgcgg agtgatcgc gaacgcgac gccggtgcc gccgacgag ttcgtggac atcctgcag gtgcaacag ctgctgttg gacgggggc cgccgcgag ccgccccca gcacaggcc gccacgtac ccgccgccc ccctattac ctaggacag gggtatgga cagccggcg gccagcgtc gacgatctc gtcgcgctt tggttcgcc ggcggaaac actgcttca gcgccgtgt tacgtcacc gcgacgtca cgttacgtt tgttggtgg tggggcgtc attggaggg gtcctgctc gcgattggc tcgcccctc gcttgcgac ttccaaata tggaacaga tactccatt cataaaagt aaagtagcg ctcgcgccg cgaccagtt tctgagaag ctcccgtgc tcggaggac gggtgtcct tctggagtc accctcgac acgagtggt gccgaggac gctagtgca gtgcgtgcc gccggcgct acaagcccc tgttctgtg gtagggccc ccggcgagc gcccccaac ccgccggcc accatgatc atggacggc ctgaacacg ctgcccgca cagcaccac caccaccac caccatcat caccactcc gccttcctg ctgaccgag tcggcggcc gccgccgcc gccgccgct caccacttc aacgtgctc agcttcgac acgtgtctg tacaagagc gtcgcctcc tcggtggac gccggcggc agccccgcg cccgccgcc gtctgcacc gccgccgac gagcccgcc gtctccgag gcgcagccc ggcgacctc aacaccccc gtcaccacc tccagcgac attccctcc ttcttcggg ccctccacc gtcgtcgaa ccgccgccc atcacaggc tcccttgac ccggaggag ctcactttg gaccaacaa cagcagcaa cagcagcag ataagtccc caccattca ccttcaggc agtcacatc ggcgaacgt gcaaccccc caagacagc gctctgagt cccagcctt cgcgaggaa gaaaacacg aaccacagc acccttagc atgtatccc acgggaaca tctcacgcg aacaacatg ggaaaaatg catcatcga accgtttac acatcagcg tcgagtcca aacatgtcg gggaacatc caaatgcag tcggttgaa ggcatggct cattctccc tcgacggtg aatccgtgg ttgttgtcc agcggggac aagccgatt tatccggcg atgttcggg cttttgggg caaggaagc tcccaatcg ccccaacag cagtacccc tcagcaacc cccagccct gcagggacg caatacgag gaacagctc atgagcatg gactgcggg ctgaaacaa cctccaagt taccccagt tgttcagct tcgacgagt ctccagtta gagttgcaa caacaacag gacttggtt tattcccgc gtcgggcag ccgctcgtg gcgagcacc gccaagtac caatgggac tcccaggag tacagtccg caaagttca agtgcgtta gtggtgccc agcacttcg aatttagta ccgaaacaa gaaccgttc agtgcgggg tgttcgagt gaaatcggc caacaatca aactcctcg tacggtgtg cagctagca gagtacaac ccttcgacg agtaaaggc cacgagatc ctctcccag gtgtaccag cagtcccca gtcccgctc aagctcgtc ccggtcaag ccccgcaag taccccaac cgccccagc aaaaccccg gtccatgag cgcccctac gcatgtccc gtggagaac tgcgaccgc cgcttttcc cgctccgac gagctcacg cgccacatc cgcatccac acgggccag aaacccttc cagtgccgc atctgcatg cggtccttc tcgcggtcg gaccacctc accacgcac attcgcacc cacacgggg gaaaagccc ttctcgtgc gacgtctgc gggaggaag ttcgcccgc agcgacgag aagaagagg cacgcgaag gtgcacctc aagcagagg atgaagaag gagagcaag atggggcag cagcagcag cagcagcag caacccccg caacagcac atgcagcac cacatgcat gccagtcac acggtgacc agtgaggac ggcctctcg gtggtgacg acgactctg tga
- >TC004846 RA
aggagaaaa aggatttct acacactgc atgaaattt tgttcagat cgccaattg ataaaaaat ctgagccac attatgacg aattgttta tttatttcc gattttttc agtgtccag ttgtttatg ttattgttt atgtgtata ggtgtgttg ttattcgtt gtcgatttc cgcggctgg caacggcat cgcgtcgaa ttgctacct ttcgaggag gcgtccgtt ggtcgtgat gacgtagcc ctgaaagag tcgtgtgcc aaccgacgg aagcagcgg gttcgaggc cgtgcgtgt gacctgggg cccggggga cccacgcac cggctgctc catgacgcc gcgcgtgac gtcaccgcc ccgcagagg cggccggtg ttggcgttg aggagcgcc gaagccgct cattcacgt gtccgtgct cgaacagcg cgcgagtgc tgtcgtcgt cgtcgtcga ggtggtgca agtgcggca tggcaccgg gaagccgct ccggcgccg gccgcttcc aagatgata gctctgatg acgatgcgc agggaacgc gacgagacg cggagtgat cgcgaacgc gacgccggt gccgccgac gagttcgtg gacatcctg caggtgcaa cagctgctg ttggacggg ggccgccgc gagccgccc ccagcacag gccgccacg tacccgccg cccccctat tacctagga caggggtat ggacagccg gcggccagc gtcgacgat ctcgtcgcg ctttggttc gccggcgga aacactgct tcagggccc ccggcgagc gcccccaac ccgccggcc accatgatc atggacggc ctgaacacg ctgcccgca cagcaccac caccaccac caccatcat caccactcc gccttcctg ctgaccgag tcggcggcc gccgccgcc gccgccgct caccacttc aacgtgctc agcttcgac acgtgtctg tacaagagc gtcgcctcc tcggtggac gccggcggc agccccgcg cccgccgcc gtctgcacc gccgccgac gagcccgcc gtctccgag gcgcagccc ggcgacctc aacaccccc gtcaccacc tccagcgac attccctcc ttcttcggg ccctccacc gtcgtcgaa ccgccgccc atcacaggc tcccttgac ccggaggag ctcactttg gaccaacaa cagcagcaa cagcagcag ataagtccc caccattca ccttcaggc agtcacatc ggcgaacgt gcaaccccc caagacagc gctctgagt cccagcctt cgcgaggaa gaaaacacg aaccacagc acccttagc atgtatccc acgggaaca tctcacgcg aacaacatg ggaaaaatg catcatcga accgtttac acatcagcg tcgagtcca aacatgtcg gggaacatc caaatgcag tcggttgaa ggcatggct cattctccc tcgacggtg aatccgtgg ttgttgtcc agcggggac aagccgatt tatccggcg atgttcggg cttttgggg caaggaagc tcccaatcg ccccaacag cagtacccc tcagcaacc cccagccct gcagggacg caatacgag gaacagctc atgagcatg gactgcggg ctgaaacaa cctccaagt taccccagt tgttcagct tcgacgagt ctccagtta gagttgcaa caacaacag gacttggtt tattcccgc gtcgggcag ccgctcgtg gcgagcacc gccaagtac caatgggac tcccaggag tacagtccg caaagttca agtgcgtta gtggtgccc agcacttcg aatttagta ccgaaacaa gaaccgttc agtgcgggg tgttcgagt gaaatcggc caacaatca aactcctcg tacggtgtg cagctagca gagtacaac ccttcgacg agtaaaggc cacgagatc ctctcccag gtgtaccag cagtcccca gtcccgctc aagctcgtc ccggtcaag ccccgcaag taccccaac cgccccagc aaaaccccg gtccatgag cgcccctac gcatgtccc gtggagaac tgcgaccgc cgcttttcc cgctccgac gagctcacg cgccacatc cgcatccac acgggccag aaacccttc cagtgccgc atctgcatg cggtccttc tcgcggtcg gaccacctc accacgcac attcgcacc cacacgggg gaaaagccc ttctcgtgc gacgtctgc gggaggaag ttcgcccgc agcgacgag aagaagagg cacgcgaag gtgcacctc aagcagagg atgaagaag gagagcaag atggggcag cagcagcag cagcagcag caacccccg caacagcac atgcagcac cacatgcat gccagtcac acggtgacc agtgaggac ggcctctcg gtggtgacg acgactctg tgagaatta cccaccgcc gagttggca cccggcgaa tgtgttctt ttcatacgc gatgactgt gatctcggc accgactcc gctcggctc atctacgtg cgaatcgaa tgaaaagca cagcgacct gaaaccatg atctccgtt ttcaagggc tggccgacg ggatacagg gcctggcga aaggttttc accaaagtt ttccttttt acaaccttg cttttgcac aactttttc aacgaaatt tccggtcat tatacaaaa aaacgtgtt tcaacttat tatacggtc caaaagtcc ttcttatct ttctcatat tttcataaa acgaatttt taactttac catcatttt aaaaaatgc actattttt atatttact aaatcttgc atttacac
- >TC004846 RB
aggagaaaa aggatttct acacactgc atgaaattt tgttcagat cgccaattg ataaaaaat ctgagccac attatgacg aattgttta tttatttcc gattttttc agtgtccag ttgtttatg ttattgttt atgtgtata ggtgtgttg ttattcgtt gtcgatttc cgcggctgg caacggcat cgcgtcgaa ttgctacct ttcgaggag gcgtccgtt ggtcgtgat gacgtagcc ctgaaagag tcgtgtgcc aaccgacgg aagcagcgg gttcgaggc cgtgcgtgt gacctgggg cccggggga cccacgcac cggctgctc catgacgcc gcgcgtgac gtcaccgcc ccgcagagg cggccggtg ttggcgttg aggagcgcc gaagccgct cattcacgt gtccgtgct cgaacagcg cgcgagtgc tgtcgtcgt cgtcgtcga ggtggtgca agtgcggca tggcaccgg gaagccgct ccggcgccg gccgcttcc aagatgata gctctgatg acgatgcgc agggaacgc gacgagacg cggagtgat cgcgaacgc gacgccggt gccgccgac gagttcgtg gacatcctg caggtgcaa cagctgctg ttggacggg ggccgccgc gagccgccc ccagcacag gccgccacg tacccgccg cccccctat tacctagga caggggtat ggacagccg gcggccagc gtcgacgat ctcgtcgcg ctttggttc gccggcgga aacactgct tcagcgccg tgttacgtc accgcgacg tcacgttac gtttgttgg tggtggggc gtcattgga ggggtcctg ctcgcgatt ggctcgccc ctcgcttgc gacttccaa atatggaac agatactcc attcataaa agtaaagta gcgctcgcg ccgcgacca gtttctgag aagctcccg tgctcggag gacgggtgt ccttctgga gtcaccctc gacacgagt ggtgccgag gacgctagt gcagtgcgt gccgccggc gctacaagc ccctgttct gtggtaggg cccccggcg agcgccccc aacccgccg gccaccatg atcatggac ggcctgaac acgctgccc gcacagcac caccaccac caccaccat catcaccac tccgccttc ctgctgacc gagtcggcg gccgccgcc gccgccgcc gctcaccac ttcaacgtg ctcagcttc gacacgtgt ctgtacaag agcgtcgcc tcctcggtg gacgccggc ggcagcccc gcgcccgcc gccgtctgc accgccgcc gacgagccc gccgtctcc gaggcgcag cccggcgac ctcaacacc cccgtcacc acctccagc gacattccc tccttcttc gggccctcc accgtcgtc gaaccgccg cccatcaca ggctccctt gacccggag gagctcact ttggaccaa caacagcag caacagcag cagataagt ccccaccat tcaccttca ggcagtcac atcggcgaa cgtgcaacc ccccaagac agcgctctg agtcccagc cttcgcgag gaagaaaac acgaaccac agcaccctt agcatgtat cccacggga acatctcac gcgaacaac atgggaaaa atgcatcat cgaaccgtt tacacatca gcgtcgagt ccaaacatg tcggggaac atccaaatg cagtcggtt gaaggcatg gctcattct ccctcgacg gtgaatccg tggttgttg tccagcggg gacaagccg atttatccg gcgatgttc gggcttttg gggcaagga agctcccaa tcgccccaa cagcagtac ccctcagca acccccagc cctgcaggg acgcaatac gaggaacag ctcatgagc atggactgc gggctgaaa caacctcca agttacccc agttgttca gcttcgacg agtctccag ttagagttg caacaacaa caggacttg gtttattcc cgcgtcggg cagccgctc gtggcgagc accgccaag taccaatgg gactcccag gagtacagt ccgcaaagt tcaagtgcg ttagtggtg cccagcact tcgaattta gtaccgaaa caagaaccg ttcagtgcg gggtgttcg agtgaaatc ggccaacaa tcaaactcc tcgtacggt gtgcagcta gcagagtac aacccttcg acgagtaaa ggccacgag atcctctcc caggtgtac cagcagtcc ccagtcccg ctcaagctc gtcccggtc aagccccgc aagtacccc aaccgcccc agcaaaacc ccggtccat gagcgcccc tacgcatgt cccgtggag aactgcgac cgccgcttt tcccgctcc gacgagctc acgcgccac atccgcatc cacacgggc cagaaaccc ttccagtgc cgcatctgc atgcggtcc ttctcgcgg tcggaccac ctcaccacg cacattcgc acccacacg ggggaaaag cccttctcg tgcgacgtc tgcgggagg aagttcgcc cgcagcgac gagaagaag aggcacgcg aaggtgcac ctcaagcag aggatgaag aaggagagc aagatgggg cagcagcag cagcagcag cagcaaccc ccgcaacag cacatgcag caccacatg catgccagt cacacggtg accagtgag gacggcctc tcggtggtg acgacgact ctgtgagaa ttacccacc gccgagttg gcacccggc gaatgtgtt cttttcata cgcgatgac tgtgatctc ggcaccgac tccgctcgg ctcatctac gtgcgaatc gaatgaaaa gcacagcga cctgaaacc atgatctcc gttttcaag ggctggccg acgggatac agggcctgg cgaaaggtt ttcaccaaa gttttcctt tttacaacc ttgcttttg cacaacttt ttcaacgaa atttccggt cattataca aaaaaacgt gtttcaact tattatacg gtccaaaag tccttctta tctttctca tattttcat aaaacgaat ttttaactt taccatcat tttaaaaaa tgcactatt tttatattt actaaatct tgcatttac ac
