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Gene information
General information
TC004846 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2989808..3002123
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004846
    iBeetle screen iB_03857

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: effect seems to be strongest in the thoracic and first abdominal segments. leg musculature seems not to be ffected at all.
    larval musculature ventral partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature lateral partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004846 RA
      MTNCLFISD FFSVQLFML LFMCIGVLL FVVDFRGWQ 
      RHRVELLPF EEASVGRDD VALKESCAN RRKQRVRGR 
      ACDLGPGGP THRLLHDAA RDVTAPQRR PVLALRSAE 
      AAHSRVRAR TARECCRRR RRGGASAAW HREAAPAPA 
      ASKMIALMT MRRERDETR SDRERDAGA ADEFVDILQ 
      VQQLLLDGG RREPPPAQA ATYPPPPYY LGQGYGQPA 
      ASVDDLVAL WFAGGNTAS GPPASAPNP PATMIMDGL 
      NTLPAQHHH HHHHHHHSA FLLTESAAA AAAAAHHFN 
      VLSFDTCLY KSVASSVDA GGSPAPAAV CTAADEPAV 
      SEAQPGDLN TPVTTSSDI PSFFGPSTV VEPPPITGS 
      LDPEELTLD QQQQQQQQI SPHHSPSGS HIGERATPQ 
      DSALSPSLR EEENTNHST LSMYPTGTS HANNMGKMH 
      HRTVYTSAS SPNMSGNIQ MQSVEGMAH SPSTVNPWL 
      LSSGDKPIY PAMFGLLGQ GSSQSPQQQ YPSATPSPA 
      GTQYEEQLM SMDCGLKQP PSYPSCSAS TSLQLELQQ 
      QQDLVYSRV GQPLVASTA KYQWDSQEY SPQSSSALV 
      VPSTSNLVP KQEPFSAGC SSEIGQQSN SSYGVQLAE 
      YNPSTSKGH EILSQVYQQ SPVPLKLVP VKPRKYPNR 
      PSKTPVHER PYACPVENC DRRFSRSDE LTRHIRIHT 
      GQKPFQCRI CMRSFSRSD HLTTHIRTH TGEKPFSCD 
      VCGRKFARS DEKKRHAKV HLKQRMKKE SKMGQQQQQ 
      QQQPPQQHM QHHMHASHT VTSEDGLSV VTTTL
    • >TC004846 RB
      MTNCLFISD FFSVQLFML LFMCIGVLL FVVDFRGWQ 
      RHRVELLPF EEASVGRDD VALKESCAN RRKQRVRGR 
      ACDLGPGGP THRLLHDAA RDVTAPQRR PVLALRSAE 
      AAHSRVRAR TARECCRRR RRGGASAAW HREAAPAPA 
      ASKMIALMT MRRERDETR SDRERDAGA ADEFVDILQ 
      VQQLLLDGG RREPPPAQA ATYPPPPYY LGQGYGQPA 
      ASVDDLVAL WFAGGNTAS APCYVTATS RYVCWWWGV 
      IGGVLLAIG SPLACDFQI WNRYSIHKS KVALAPRPV 
      SEKLPCSED GCPSGVTLD TSGAEDASA VRAAGATSP 
      CSVVGPPAS APNPPATMI MDGLNTLPA QHHHHHHHH 
      HHSAFLLTE SAAAAAAAA HHFNVLSFD TCLYKSVAS 
      SVDAGGSPA PAAVCTAAD EPAVSEAQP GDLNTPVTT 
      SSDIPSFFG PSTVVEPPP ITGSLDPEE LTLDQQQQQ 
      QQQISPHHS PSGSHIGER ATPQDSALS PSLREEENT 
      NHSTLSMYP TGTSHANNM GKMHHRTVY TSASSPNMS 
      GNIQMQSVE GMAHSPSTV NPWLLSSGD KPIYPAMFG 
      LLGQGSSQS PQQQYPSAT PSPAGTQYE EQLMSMDCG 
      LKQPPSYPS CSASTSLQL ELQQQQDLV YSRVGQPLV 
      ASTAKYQWD SQEYSPQSS SALVVPSTS NLVPKQEPF 
      SAGCSSEIG QQSNSSYGV QLAEYNPST SKGHEILSQ 
      VYQQSPVPL KLVPVKPRK YPNRPSKTP VHERPYACP 
      VENCDRRFS RSDELTRHI RIHTGQKPF QCRICMRSF 
      SRSDHLTTH IRTHTGEKP FSCDVCGRK FARSDEKKR 
      HAKVHLKQR MKKESKMGQ QQQQQQQPP QQHMQHHMH 
      ASHTVTSED GLSVVTTTL
    CDS sequence
    • >TC004846 RA
      atgacgaat tgtttattt atttccgat tttttcagt 
      gtccagttg tttatgtta ttgtttatg tgtataggt 
      gtgttgtta ttcgttgtc gatttccgc ggctggcaa 
      cggcatcgc gtcgaattg ctacctttc gaggaggcg 
      tccgttggt cgtgatgac gtagccctg aaagagtcg 
      tgtgccaac cgacggaag cagcgggtt cgaggccgt 
      gcgtgtgac ctggggccc gggggaccc acgcaccgg 
      ctgctccat gacgccgcg cgtgacgtc accgccccg 
      cagaggcgg ccggtgttg gcgttgagg agcgccgaa 
      gccgctcat tcacgtgtc cgtgctcga acagcgcgc 
      gagtgctgt cgtcgtcgt cgtcgaggt ggtgcaagt 
      gcggcatgg caccgggaa gccgctccg gcgccggcc 
      gcttccaag atgatagct ctgatgacg atgcgcagg 
      gaacgcgac gagacgcgg agtgatcgc gaacgcgac 
      gccggtgcc gccgacgag ttcgtggac atcctgcag 
      gtgcaacag ctgctgttg gacgggggc cgccgcgag 
      ccgccccca gcacaggcc gccacgtac ccgccgccc 
      ccctattac ctaggacag gggtatgga cagccggcg 
      gccagcgtc gacgatctc gtcgcgctt tggttcgcc 
      ggcggaaac actgcttca gggcccccg gcgagcgcc 
      cccaacccg ccggccacc atgatcatg gacggcctg 
      aacacgctg cccgcacag caccaccac caccaccac 
      catcatcac cactccgcc ttcctgctg accgagtcg 
      gcggccgcc gccgccgcc gccgctcac cacttcaac 
      gtgctcagc ttcgacacg tgtctgtac aagagcgtc 
      gcctcctcg gtggacgcc ggcggcagc cccgcgccc 
      gccgccgtc tgcaccgcc gccgacgag cccgccgtc 
      tccgaggcg cagcccggc gacctcaac acccccgtc 
      accacctcc agcgacatt ccctccttc ttcgggccc 
      tccaccgtc gtcgaaccg ccgcccatc acaggctcc 
      cttgacccg gaggagctc actttggac caacaacag 
      cagcaacag cagcagata agtccccac cattcacct 
      tcaggcagt cacatcggc gaacgtgca accccccaa 
      gacagcgct ctgagtccc agccttcgc gaggaagaa 
      aacacgaac cacagcacc cttagcatg tatcccacg 
      ggaacatct cacgcgaac aacatggga aaaatgcat 
      catcgaacc gtttacaca tcagcgtcg agtccaaac 
      atgtcgggg aacatccaa atgcagtcg gttgaaggc 
      atggctcat tctccctcg acggtgaat ccgtggttg 
      ttgtccagc ggggacaag ccgatttat ccggcgatg 
      ttcgggctt ttggggcaa ggaagctcc caatcgccc 
      caacagcag tacccctca gcaaccccc agccctgca 
      gggacgcaa tacgaggaa cagctcatg agcatggac 
      tgcgggctg aaacaacct ccaagttac cccagttgt 
      tcagcttcg acgagtctc cagttagag ttgcaacaa 
      caacaggac ttggtttat tcccgcgtc gggcagccg 
      ctcgtggcg agcaccgcc aagtaccaa tgggactcc 
      caggagtac agtccgcaa agttcaagt gcgttagtg 
      gtgcccagc acttcgaat ttagtaccg aaacaagaa 
      ccgttcagt gcggggtgt tcgagtgaa atcggccaa 
      caatcaaac tcctcgtac ggtgtgcag ctagcagag 
      tacaaccct tcgacgagt aaaggccac gagatcctc 
      tcccaggtg taccagcag tccccagtc ccgctcaag 
      ctcgtcccg gtcaagccc cgcaagtac cccaaccgc 
      cccagcaaa accccggtc catgagcgc ccctacgca 
      tgtcccgtg gagaactgc gaccgccgc ttttcccgc 
      tccgacgag ctcacgcgc cacatccgc atccacacg 
      ggccagaaa cccttccag tgccgcatc tgcatgcgg 
      tccttctcg cggtcggac cacctcacc acgcacatt 
      cgcacccac acgggggaa aagcccttc tcgtgcgac 
      gtctgcggg aggaagttc gcccgcagc gacgagaag 
      aagaggcac gcgaaggtg cacctcaag cagaggatg 
      aagaaggag agcaagatg gggcagcag cagcagcag 
      cagcagcaa cccccgcaa cagcacatg cagcaccac 
      atgcatgcc agtcacacg gtgaccagt gaggacggc 
      ctctcggtg gtgacgacg actctgtga
    • >TC004846 RB
      atgacgaat tgtttattt atttccgat tttttcagt 
      gtccagttg tttatgtta ttgtttatg tgtataggt 
      gtgttgtta ttcgttgtc gatttccgc ggctggcaa 
      cggcatcgc gtcgaattg ctacctttc gaggaggcg 
      tccgttggt cgtgatgac gtagccctg aaagagtcg 
      tgtgccaac cgacggaag cagcgggtt cgaggccgt 
      gcgtgtgac ctggggccc gggggaccc acgcaccgg 
      ctgctccat gacgccgcg cgtgacgtc accgccccg 
      cagaggcgg ccggtgttg gcgttgagg agcgccgaa 
      gccgctcat tcacgtgtc cgtgctcga acagcgcgc 
      gagtgctgt cgtcgtcgt cgtcgaggt ggtgcaagt 
      gcggcatgg caccgggaa gccgctccg gcgccggcc 
      gcttccaag atgatagct ctgatgacg atgcgcagg 
      gaacgcgac gagacgcgg agtgatcgc gaacgcgac 
      gccggtgcc gccgacgag ttcgtggac atcctgcag 
      gtgcaacag ctgctgttg gacgggggc cgccgcgag 
      ccgccccca gcacaggcc gccacgtac ccgccgccc 
      ccctattac ctaggacag gggtatgga cagccggcg 
      gccagcgtc gacgatctc gtcgcgctt tggttcgcc 
      ggcggaaac actgcttca gcgccgtgt tacgtcacc 
      gcgacgtca cgttacgtt tgttggtgg tggggcgtc 
      attggaggg gtcctgctc gcgattggc tcgcccctc 
      gcttgcgac ttccaaata tggaacaga tactccatt 
      cataaaagt aaagtagcg ctcgcgccg cgaccagtt 
      tctgagaag ctcccgtgc tcggaggac gggtgtcct 
      tctggagtc accctcgac acgagtggt gccgaggac 
      gctagtgca gtgcgtgcc gccggcgct acaagcccc 
      tgttctgtg gtagggccc ccggcgagc gcccccaac 
      ccgccggcc accatgatc atggacggc ctgaacacg 
      ctgcccgca cagcaccac caccaccac caccatcat 
      caccactcc gccttcctg ctgaccgag tcggcggcc 
      gccgccgcc gccgccgct caccacttc aacgtgctc 
      agcttcgac acgtgtctg tacaagagc gtcgcctcc 
      tcggtggac gccggcggc agccccgcg cccgccgcc 
      gtctgcacc gccgccgac gagcccgcc gtctccgag 
      gcgcagccc ggcgacctc aacaccccc gtcaccacc 
      tccagcgac attccctcc ttcttcggg ccctccacc 
      gtcgtcgaa ccgccgccc atcacaggc tcccttgac 
      ccggaggag ctcactttg gaccaacaa cagcagcaa 
      cagcagcag ataagtccc caccattca ccttcaggc 
      agtcacatc ggcgaacgt gcaaccccc caagacagc 
      gctctgagt cccagcctt cgcgaggaa gaaaacacg 
      aaccacagc acccttagc atgtatccc acgggaaca 
      tctcacgcg aacaacatg ggaaaaatg catcatcga 
      accgtttac acatcagcg tcgagtcca aacatgtcg 
      gggaacatc caaatgcag tcggttgaa ggcatggct 
      cattctccc tcgacggtg aatccgtgg ttgttgtcc 
      agcggggac aagccgatt tatccggcg atgttcggg 
      cttttgggg caaggaagc tcccaatcg ccccaacag 
      cagtacccc tcagcaacc cccagccct gcagggacg 
      caatacgag gaacagctc atgagcatg gactgcggg 
      ctgaaacaa cctccaagt taccccagt tgttcagct 
      tcgacgagt ctccagtta gagttgcaa caacaacag 
      gacttggtt tattcccgc gtcgggcag ccgctcgtg 
      gcgagcacc gccaagtac caatgggac tcccaggag 
      tacagtccg caaagttca agtgcgtta gtggtgccc 
      agcacttcg aatttagta ccgaaacaa gaaccgttc 
      agtgcgggg tgttcgagt gaaatcggc caacaatca 
      aactcctcg tacggtgtg cagctagca gagtacaac 
      ccttcgacg agtaaaggc cacgagatc ctctcccag 
      gtgtaccag cagtcccca gtcccgctc aagctcgtc 
      ccggtcaag ccccgcaag taccccaac cgccccagc 
      aaaaccccg gtccatgag cgcccctac gcatgtccc 
      gtggagaac tgcgaccgc cgcttttcc cgctccgac 
      gagctcacg cgccacatc cgcatccac acgggccag 
      aaacccttc cagtgccgc atctgcatg cggtccttc 
      tcgcggtcg gaccacctc accacgcac attcgcacc 
      cacacgggg gaaaagccc ttctcgtgc gacgtctgc 
      gggaggaag ttcgcccgc agcgacgag aagaagagg 
      cacgcgaag gtgcacctc aagcagagg atgaagaag 
      gagagcaag atggggcag cagcagcag cagcagcag 
      caacccccg caacagcac atgcagcac cacatgcat 
      gccagtcac acggtgacc agtgaggac ggcctctcg 
      gtggtgacg acgactctg tga
    mRNA sequence
    • >TC004846 RA
      aggagaaaa aggatttct acacactgc atgaaattt 
      tgttcagat cgccaattg ataaaaaat ctgagccac 
      attatgacg aattgttta tttatttcc gattttttc 
      agtgtccag ttgtttatg ttattgttt atgtgtata 
      ggtgtgttg ttattcgtt gtcgatttc cgcggctgg 
      caacggcat cgcgtcgaa ttgctacct ttcgaggag 
      gcgtccgtt ggtcgtgat gacgtagcc ctgaaagag 
      tcgtgtgcc aaccgacgg aagcagcgg gttcgaggc 
      cgtgcgtgt gacctgggg cccggggga cccacgcac 
      cggctgctc catgacgcc gcgcgtgac gtcaccgcc 
      ccgcagagg cggccggtg ttggcgttg aggagcgcc 
      gaagccgct cattcacgt gtccgtgct cgaacagcg 
      cgcgagtgc tgtcgtcgt cgtcgtcga ggtggtgca 
      agtgcggca tggcaccgg gaagccgct ccggcgccg 
      gccgcttcc aagatgata gctctgatg acgatgcgc 
      agggaacgc gacgagacg cggagtgat cgcgaacgc 
      gacgccggt gccgccgac gagttcgtg gacatcctg 
      caggtgcaa cagctgctg ttggacggg ggccgccgc 
      gagccgccc ccagcacag gccgccacg tacccgccg 
      cccccctat tacctagga caggggtat ggacagccg 
      gcggccagc gtcgacgat ctcgtcgcg ctttggttc 
      gccggcgga aacactgct tcagggccc ccggcgagc 
      gcccccaac ccgccggcc accatgatc atggacggc 
      ctgaacacg ctgcccgca cagcaccac caccaccac 
      caccatcat caccactcc gccttcctg ctgaccgag 
      tcggcggcc gccgccgcc gccgccgct caccacttc 
      aacgtgctc agcttcgac acgtgtctg tacaagagc 
      gtcgcctcc tcggtggac gccggcggc agccccgcg 
      cccgccgcc gtctgcacc gccgccgac gagcccgcc 
      gtctccgag gcgcagccc ggcgacctc aacaccccc 
      gtcaccacc tccagcgac attccctcc ttcttcggg 
      ccctccacc gtcgtcgaa ccgccgccc atcacaggc 
      tcccttgac ccggaggag ctcactttg gaccaacaa 
      cagcagcaa cagcagcag ataagtccc caccattca 
      ccttcaggc agtcacatc ggcgaacgt gcaaccccc 
      caagacagc gctctgagt cccagcctt cgcgaggaa 
      gaaaacacg aaccacagc acccttagc atgtatccc 
      acgggaaca tctcacgcg aacaacatg ggaaaaatg 
      catcatcga accgtttac acatcagcg tcgagtcca 
      aacatgtcg gggaacatc caaatgcag tcggttgaa 
      ggcatggct cattctccc tcgacggtg aatccgtgg 
      ttgttgtcc agcggggac aagccgatt tatccggcg 
      atgttcggg cttttgggg caaggaagc tcccaatcg 
      ccccaacag cagtacccc tcagcaacc cccagccct 
      gcagggacg caatacgag gaacagctc atgagcatg 
      gactgcggg ctgaaacaa cctccaagt taccccagt 
      tgttcagct tcgacgagt ctccagtta gagttgcaa 
      caacaacag gacttggtt tattcccgc gtcgggcag 
      ccgctcgtg gcgagcacc gccaagtac caatgggac 
      tcccaggag tacagtccg caaagttca agtgcgtta 
      gtggtgccc agcacttcg aatttagta ccgaaacaa 
      gaaccgttc agtgcgggg tgttcgagt gaaatcggc 
      caacaatca aactcctcg tacggtgtg cagctagca 
      gagtacaac ccttcgacg agtaaaggc cacgagatc 
      ctctcccag gtgtaccag cagtcccca gtcccgctc 
      aagctcgtc ccggtcaag ccccgcaag taccccaac 
      cgccccagc aaaaccccg gtccatgag cgcccctac 
      gcatgtccc gtggagaac tgcgaccgc cgcttttcc 
      cgctccgac gagctcacg cgccacatc cgcatccac 
      acgggccag aaacccttc cagtgccgc atctgcatg 
      cggtccttc tcgcggtcg gaccacctc accacgcac 
      attcgcacc cacacgggg gaaaagccc ttctcgtgc 
      gacgtctgc gggaggaag ttcgcccgc agcgacgag 
      aagaagagg cacgcgaag gtgcacctc aagcagagg 
      atgaagaag gagagcaag atggggcag cagcagcag 
      cagcagcag caacccccg caacagcac atgcagcac 
      cacatgcat gccagtcac acggtgacc agtgaggac 
      ggcctctcg gtggtgacg acgactctg tgagaatta 
      cccaccgcc gagttggca cccggcgaa tgtgttctt 
      ttcatacgc gatgactgt gatctcggc accgactcc 
      gctcggctc atctacgtg cgaatcgaa tgaaaagca 
      cagcgacct gaaaccatg atctccgtt ttcaagggc 
      tggccgacg ggatacagg gcctggcga aaggttttc 
      accaaagtt ttccttttt acaaccttg cttttgcac 
      aactttttc aacgaaatt tccggtcat tatacaaaa 
      aaacgtgtt tcaacttat tatacggtc caaaagtcc 
      ttcttatct ttctcatat tttcataaa acgaatttt 
      taactttac catcatttt aaaaaatgc actattttt 
      atatttact aaatcttgc atttacac
    • >TC004846 RB
      aggagaaaa aggatttct acacactgc atgaaattt 
      tgttcagat cgccaattg ataaaaaat ctgagccac 
      attatgacg aattgttta tttatttcc gattttttc 
      agtgtccag ttgtttatg ttattgttt atgtgtata 
      ggtgtgttg ttattcgtt gtcgatttc cgcggctgg 
      caacggcat cgcgtcgaa ttgctacct ttcgaggag 
      gcgtccgtt ggtcgtgat gacgtagcc ctgaaagag 
      tcgtgtgcc aaccgacgg aagcagcgg gttcgaggc 
      cgtgcgtgt gacctgggg cccggggga cccacgcac 
      cggctgctc catgacgcc gcgcgtgac gtcaccgcc 
      ccgcagagg cggccggtg ttggcgttg aggagcgcc 
      gaagccgct cattcacgt gtccgtgct cgaacagcg 
      cgcgagtgc tgtcgtcgt cgtcgtcga ggtggtgca 
      agtgcggca tggcaccgg gaagccgct ccggcgccg 
      gccgcttcc aagatgata gctctgatg acgatgcgc 
      agggaacgc gacgagacg cggagtgat cgcgaacgc 
      gacgccggt gccgccgac gagttcgtg gacatcctg 
      caggtgcaa cagctgctg ttggacggg ggccgccgc 
      gagccgccc ccagcacag gccgccacg tacccgccg 
      cccccctat tacctagga caggggtat ggacagccg 
      gcggccagc gtcgacgat ctcgtcgcg ctttggttc 
      gccggcgga aacactgct tcagcgccg tgttacgtc 
      accgcgacg tcacgttac gtttgttgg tggtggggc 
      gtcattgga ggggtcctg ctcgcgatt ggctcgccc 
      ctcgcttgc gacttccaa atatggaac agatactcc 
      attcataaa agtaaagta gcgctcgcg ccgcgacca 
      gtttctgag aagctcccg tgctcggag gacgggtgt 
      ccttctgga gtcaccctc gacacgagt ggtgccgag 
      gacgctagt gcagtgcgt gccgccggc gctacaagc 
      ccctgttct gtggtaggg cccccggcg agcgccccc 
      aacccgccg gccaccatg atcatggac ggcctgaac 
      acgctgccc gcacagcac caccaccac caccaccat 
      catcaccac tccgccttc ctgctgacc gagtcggcg 
      gccgccgcc gccgccgcc gctcaccac ttcaacgtg 
      ctcagcttc gacacgtgt ctgtacaag agcgtcgcc 
      tcctcggtg gacgccggc ggcagcccc gcgcccgcc 
      gccgtctgc accgccgcc gacgagccc gccgtctcc 
      gaggcgcag cccggcgac ctcaacacc cccgtcacc 
      acctccagc gacattccc tccttcttc gggccctcc 
      accgtcgtc gaaccgccg cccatcaca ggctccctt 
      gacccggag gagctcact ttggaccaa caacagcag 
      caacagcag cagataagt ccccaccat tcaccttca 
      ggcagtcac atcggcgaa cgtgcaacc ccccaagac 
      agcgctctg agtcccagc cttcgcgag gaagaaaac 
      acgaaccac agcaccctt agcatgtat cccacggga 
      acatctcac gcgaacaac atgggaaaa atgcatcat 
      cgaaccgtt tacacatca gcgtcgagt ccaaacatg 
      tcggggaac atccaaatg cagtcggtt gaaggcatg 
      gctcattct ccctcgacg gtgaatccg tggttgttg 
      tccagcggg gacaagccg atttatccg gcgatgttc 
      gggcttttg gggcaagga agctcccaa tcgccccaa 
      cagcagtac ccctcagca acccccagc cctgcaggg 
      acgcaatac gaggaacag ctcatgagc atggactgc 
      gggctgaaa caacctcca agttacccc agttgttca 
      gcttcgacg agtctccag ttagagttg caacaacaa 
      caggacttg gtttattcc cgcgtcggg cagccgctc 
      gtggcgagc accgccaag taccaatgg gactcccag 
      gagtacagt ccgcaaagt tcaagtgcg ttagtggtg 
      cccagcact tcgaattta gtaccgaaa caagaaccg 
      ttcagtgcg gggtgttcg agtgaaatc ggccaacaa 
      tcaaactcc tcgtacggt gtgcagcta gcagagtac 
      aacccttcg acgagtaaa ggccacgag atcctctcc 
      caggtgtac cagcagtcc ccagtcccg ctcaagctc 
      gtcccggtc aagccccgc aagtacccc aaccgcccc 
      agcaaaacc ccggtccat gagcgcccc tacgcatgt 
      cccgtggag aactgcgac cgccgcttt tcccgctcc 
      gacgagctc acgcgccac atccgcatc cacacgggc 
      cagaaaccc ttccagtgc cgcatctgc atgcggtcc 
      ttctcgcgg tcggaccac ctcaccacg cacattcgc 
      acccacacg ggggaaaag cccttctcg tgcgacgtc 
      tgcgggagg aagttcgcc cgcagcgac gagaagaag 
      aggcacgcg aaggtgcac ctcaagcag aggatgaag 
      aaggagagc aagatgggg cagcagcag cagcagcag 
      cagcaaccc ccgcaacag cacatgcag caccacatg 
      catgccagt cacacggtg accagtgag gacggcctc 
      tcggtggtg acgacgact ctgtgagaa ttacccacc 
      gccgagttg gcacccggc gaatgtgtt cttttcata 
      cgcgatgac tgtgatctc ggcaccgac tccgctcgg 
      ctcatctac gtgcgaatc gaatgaaaa gcacagcga 
      cctgaaacc atgatctcc gttttcaag ggctggccg 
      acgggatac agggcctgg cgaaaggtt ttcaccaaa 
      gttttcctt tttacaacc ttgcttttg cacaacttt 
      ttcaacgaa atttccggt cattataca aaaaaacgt 
      gtttcaact tattatacg gtccaaaag tccttctta 
      tctttctca tattttcat aaaacgaat ttttaactt 
      taccatcat tttaaaaaa tgcactatt tttatattt 
      actaaatct tgcatttac ac
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