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Gene information
General information
TC004840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 619619..640785
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004840
    iBeetle screen iB_03856
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004840 RA
      MFMFRSRRA TLTKRLLKA RRRRGAPDD DGDGVMVLL 
      KQLQENQLG MLWTAVESR GRDGGSNCV LMPRDRQPH 
      VLCCQTWRW PDLLQSTDL KRLPACRFA GDPVYVCCN 
      PYHWSRVYQ PDTPPPPYS KSEKLDRAP SENPLRQQF 
      HGSLTTNGE DSLRNPSEW CRLAYWELA QRVGPLFPV 
      EAPAVNVFG DVPYCDGLS LETLAQQNC NAPESVRHG 
      RCKIGLGVT LSHEGGSVW VYNRSENPI FVNSVTLDS 
      ADSPLPTRV PAEQCLCVY DPQKAAAHR TCWDFSTHG 
      PIDPNSIRI SFAKGWGPL YKRQEITSC PCWLEILLA 
      PCR
    CDS sequence
    • >TC004840 RA
      atgttcatg ttccggagc agacgggcc actctgacc 
      aaacggctg ctgaaggcg cgcagacga cggggggcc 
      cccgacgac gatggtgac ggcgtgatg gtgctcctc 
      aaacagctc caggagaac cagctgggg atgctgtgg 
      acggcggtg gagagcaga ggccgagat ggaggcagc 
      aattgcgtg ctgatgccc agagaccga cagccgcac 
      gtgctgtgt tgccagacg tggcggtgg ccggatctg 
      ctccagagc acggatctg aagaggttg ccggcgtgc 
      aggttcgca ggagacccc gtctacgtc tgctgcaac 
      ccctaccac tggagccgc gtctaccaa cccgatacc 
      ccgcctcct ccctatagc aaatcggag aaattagat 
      cgagcacca agcgaaaac cctcttcgc cagcagttc 
      catgggtct ttgacgacg aacggagaa gattcgttg 
      cgaaatcct tcggaatgg tgcagattg gcttattgg 
      gagttggcc caacgggtg ggtccgctc tttcctgtg 
      gaagccccg gctgtgaat gtcttcggt gacgtaccc 
      tattgcgat ggtctgtcg ttagaaacg ttagctcaa 
      caaaattgc aacgcccca gagtctgtc agacatgga 
      aggtgtaaa attggctta ggcgtgaca ctgtcgcat 
      gaaggcggc tccgtgtgg gtgtacaac cgctcggag 
      aacccgatc ttcgtgaat tctgtgact ctggacagc 
      gccgattcc cctttacct actcgagta cctgccgag 
      caatgcctc tgcgtctac gacccccag aaggcagca 
      gctcaccga acgtgttgg gacttcagc acgcacggc 
      cccatcgac cccaactcg atacgaatc agcttcgcc 
      aagggctgg ggcccgctg tacaagcgg caagagata 
      acgtcgtgt ccgtgctgg ttagaaata cttttagcg 
      ccgtgcagg tga
    mRNA sequence
    • >TC004840 RA
      cgacgagag tcgaagtta tccgatcgt ctctcgccg 
      catcttcag tgctgccag cctcgtcag ttcagtgac 
      aaccaagca agtgtaacg agtgatggg tgtttatcg 
      cccgtcctc cggtgtccg gtagtagta ttgttgtaa 
      aatccaagt gcgatgatg gagtgtggt ctagtgtga 
      gtgcagtgg gcggcggcg gcggcgagg agtcaccag 
      tggccccca aatgctggt tacatgttc atgttccgg 
      agcagacgg gccactctg accaaacgg ctgctgaag 
      gcgcgcaga cgacggggg gcccccgac gacgatggt 
      gacggcgtg atggtgctc ctcaaacag ctccaggag 
      aaccagctg gggatgctg tggacggcg gtggagagc 
      agaggccga gatggaggc agcaattgc gtgctgatg 
      cccagagac cgacagccg cacgtgctg tgttgccag 
      acgtggcgg tggccggat ctgctccag agcacggat 
      ctgaagagg ttgccggcg tgcaggttc gcaggagac 
      cccgtctac gtctgctgc aacccctac cactggagc 
      cgcgtctac caacccgat accccgcct cctccctat 
      agcaaatcg gagaaatta gatcgagca ccaagcgaa 
      aaccctctt cgccagcag ttccatggg tctttgacg 
      acgaacgga gaagattcg ttgcgaaat ccttcggaa 
      tggtgcaga ttggcttat tgggagttg gcccaacgg 
      gtgggtccg ctctttcct gtggaagcc ccggctgtg 
      aatgtcttc ggtgacgta ccctattgc gatggtctg 
      tcgttagaa acgttagct caacaaaat tgcaacgcc 
      ccagagtct gtcagacat ggaaggtgt aaaattggc 
      ttaggcgtg acactgtcg catgaaggc ggctccgtg 
      tgggtgtac aaccgctcg gagaacccg atcttcgtg 
      aattctgtg actctggac agcgccgat tccccttta 
      cctactcga gtacctgcc gagcaatgc ctctgcgtc 
      tacgacccc cagaaggca gcagctcac cgaacgtgt 
      tgggacttc agcacgcac ggccccatc gaccccaac 
      tcgatacga atcagcttc gccaagggc tggggcccg 
      ctgtacaag cggcaagag ataacgtcg tgtccgtgc 
      tggttagaa atactttta gcgccgtgc aggtgatca 
      aaagacact cctccaaag tgctgttgt actcaagtg 
      gaactcagt gatgacgac gatcacaag gctgaattt 
      ttcaacttg ttgaaggag cggtgctgt gctgtttcc 
      gtcctacta ttcccgagt ttgaggctg atgcggtgc 
      ctggccaca gggtaaatg gaagccaag gaaataagg 
      actttaaca aatttttcg gcccaccaa tatttttta 
      tacgattct ctggtttat tttatttat tgtgcaaaa 
      accgggtgc ttaaagcag gtttcgaat ttactggga 
      tacgaacca caaatctgc tgtgagcat ccacgaaat 
      taccgacct gcaaattat gtgagagta gaagtactg 
      atgtgtgtc gtaaaggcc ttataaaca tcgttcaaa 
      cgatgcgcg ttattaagg aatttattg aagcgaaag 
      aggagcttc aagtcgtgg gggaccata cgttaatca 
      atttcaggt taattttat taataattt ttaaaaaag 
      catttttat ttttgtata attatggtc aattgttgt 
      tttccttct ctgtactta ttttattaa ttgtgaatg 
      caattggta ataatagta ttgcatttt gtcccactt 
      ttttgtata ttttgtatt aaccagcaa gggtcttca 
      ttttattgt ctttatgga aaacttact agtcattaa 
      aaagaaatt tcgcgctag aatgtaggt ccatgcctg 
      ttcataata tgtaaaaat atgttccaa gttatgaaa 
      atgtatatg ttacagaat ttatataaa gtgtttaaa 
      attgtaaaa atagcagtg ccttgatag caatcatat 
      tatgaaaaa tacataagt actgtactt tctgcatta 
      tgtaggaaa tattaattg gttttattt atttattaa 
      atttttaaa ttatacatt tttgtatat caaataaat 
      ttatgtata aaat