| General information | |||
| TC004836 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2664159..2666721 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004836 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03855 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC004836 RA
MLFKSTEAS KHIEANPHA KRLYDDLLS NYNRLIRPV ENHTQTLTV WLGLKLSQL IEMNLKNQV MTTNLWVVQ RWYDYKLKW EPEEYGGVE MLYVPSEHI WLPDIVLFN NADGNYEVT LMTKATLSY TGEVIWKPP SIYKSSCEI NVQYFPFDE QSCLMKFGS WTYNGLQVD LKHMDQVSG NNIVKVGID LSEFYLSVE WDILAVPAT RNEEYYPCC TEPYSDITF KITMRRKTL FYTVNLIIP CVGITFLTV LVFYLPSDS GEKVTLCVS ILLSLTVFF LLLAEIIPP TSLAVPLLG KYLLFTMIL VTSSIWVTV CVLNVHFRS PSTHKMSPI VRKIFLQFM PKLLIMRRT TYTLPEYDD SLPPRGYSG EIEMQLNMR DPFNTDFKM KAHSSMSSS NITPRFLSA NVLSALQGV RFIAQHIRD ADKDNEIVE DWKFVSMVL DRFFLWVFT LACIGGTFG IILQAPSLY DTRVPVHQQ LSEIPLTKI FQIPPSRTH IPTQ
- >TC004836 RA
atgctgttt aaatccact gaagcctcc aaacacatc gaggctaat cctcatgct aagcgatta tacgacgac ctcctcagt aattacaat cggctgatc agacctgtg gaaaaccat acgcaaact ctaactgta tggctgggc ctaaaactg tcacaattg atcgaaatg aacctaaaa aatcaagtc atgacgacc aatttatgg gtggtgcag agatggtac gactataaa ttaaagtgg gaaccggag gaatatggc ggtgtggaa atgctttac gtcccttct gagcatatt tggcttccg gacatcgtt ttattcaat aatgccgat ggtaattat gaggtgact ttgatgact aaagccact ttgtcttac accggcgaa gtaatttgg aagccacct tcgatctat aaatcttct tgcgaaata aacgtgcaa tattttccc ttcgacgag caaagttgt ctaatgaaa ttcggttct tggacttac aatggcctt caagtcgac ttgaagcac atggaccaa gtctccggc aacaatatt gtcaaagtc ggtattgac ttgtccgaa ttttacctt tccgtcgaa tgggacatc cttgcagtc cccgccacg cgaaacgaa gagtactat ccttgttgc acagagccg tattcagat atcactttc aagataaca atgcgtcgc aagacatta ttctataca gtcaacttg atcattcct tgcgtcggg atcacattt cttacagtt cttgttttc taccttcct tcggattcg ggcgagaaa gtcacactt tgtgtctca atcctatta tctctaact gtgtttttc ctgttgctc gctgaaatt attccgccg acttcgttg gccgtccct ttgttgggc aaatacctc ctctttacc atgattttg gtcacttcg tccatatgg gtcacggtg tgcgtgctc aacgtgcat ttcagatcc ccttcgact cacaaaatg tcgccgatt gtgcgaaaa atcttcctc caattcatg cccaaactg ctgattatg cggcggacg acttacact ttgcctgag tatgacgac agtttgcct cccagagga tacagtggc gagatcgaa atgcaattg aatatgaga gatccattc aacactgat ttcaaaatg aaagcacat tcgtcgatg tcctcctcg aatataact cctcgtttc ctgtcggcg aacgttctc tcggctctg cagggtgtt aggtttatt gcacaacac atcagagat gccgataaa gataatgaa atcgttgaa gattggaag tttgtttcg atggtccta gatcgattc tttttatgg gtcttcact ctggcttgc atcggcgga actttcggg attatatta caagctcct tccttgtac gacacccga gtgcctgtt catcaacaa cttagcgaa attcctctc acgaaaatt ttccaaata cccccctcg cgcacacac atccccaca caataa
- >TC004836 RA
cctggcgat gtgggagta aaacaacat ttccttaat tagcctagt tatgtattt aataaaatt agtttgttt cactttcat gctgtttaa atccactga agcctccaa acacatcga ggctaatcc tcatgctaa gcgattata cgacgacct cctcagtaa ttacaatcg gctgatcag acctgtgga aaaccatac gcaaactct aactgtatg gctgggcct aaaactgtc acaattgat cgaaatgaa cctaaaaaa tcaagtcat gacgaccaa tttatgggt ggtgcagag atggtacga ctataaatt aaagtggga accggagga atatggcgg tgtggaaat gctttacgt cccttctga gcatatttg gcttccgga catcgtttt attcaataa tgccgatgg taattatga ggtgacttt gatgactaa agccacttt gtcttacac cggcgaagt aatttggaa gccaccttc gatctataa atcttcttg cgaaataaa cgtgcaata ttttccctt cgacgagca aagttgtct aatgaaatt cggttcttg gacttacaa tggccttca agtcgactt gaagcacat ggaccaagt ctccggcaa caatattgt caaagtcgg tattgactt gtccgaatt ttacctttc cgtcgaatg ggacatcct tgcagtccc cgccacgcg aaacgaaga gtactatcc ttgttgcac agagccgta ttcagatat cactttcaa gataacaat gcgtcgcaa gacattatt ctatacagt caacttgat cattccttg cgtcgggat cacatttct tacagttct tgttttcta ccttccttc ggattcggg cgagaaagt cacactttg tgtctcaat cctattatc tctaactgt gtttttcct gttgctcgc tgaaattat tccgccgac ttcgttggc cgtcccttt gttgggcaa atacctcct ctttaccat gattttggt cacttcgtc catatgggt cacggtgtg cgtgctcaa cgtgcattt cagatcccc ttcgactca caaaatgtc gccgattgt gcgaaaaat cttcctcca attcatgcc caaactgct gattatgcg gcggacgac ttacacttt gcctgagta tgacgacag tttgcctcc cagaggata cagtggcga gatcgaaat gcaattgaa tatgagaga tccattcaa cactgattt caaaatgaa agcacattc gtcgatgtc ctcctcgaa tataactcc tcgtttcct gtcggcgaa cgttctctc ggctctgca gggtgttag gtttattgc acaacacat cagagatgc cgataaaga taatgaaat cgttgaaga ttggaagtt tgtttcgat ggtcctaga tcgattctt tttatgggt cttcactct ggcttgcat cggcggaac tttcgggat tatattaca agctccttc cttgtacga cacccgagt gcctgttca tcaacaact tagcgaaat tcctctcac gaaaatttt ccaaatacc cccctcgcg cacacacat ccccacaca ataaaccac agggtataa ccaaaaaac cttcttcct ctaaatggg accattgca caattctaa caaagcaca actactttt tgaatgttt tatggggca attttccaa aaatcgcac aatttttta cccaaacac ttcacactt ttgtggacc tagcctctg ttaccaaat gttatatgt gcttaaaat acaataaaa accgttaga acat
