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Gene information
General information
TC004836 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2664159..2666721
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004836
    iBeetle screen iB_03855

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004836 RA
      MLFKSTEAS KHIEANPHA KRLYDDLLS NYNRLIRPV 
      ENHTQTLTV WLGLKLSQL IEMNLKNQV MTTNLWVVQ 
      RWYDYKLKW EPEEYGGVE MLYVPSEHI WLPDIVLFN 
      NADGNYEVT LMTKATLSY TGEVIWKPP SIYKSSCEI 
      NVQYFPFDE QSCLMKFGS WTYNGLQVD LKHMDQVSG 
      NNIVKVGID LSEFYLSVE WDILAVPAT RNEEYYPCC 
      TEPYSDITF KITMRRKTL FYTVNLIIP CVGITFLTV 
      LVFYLPSDS GEKVTLCVS ILLSLTVFF LLLAEIIPP 
      TSLAVPLLG KYLLFTMIL VTSSIWVTV CVLNVHFRS 
      PSTHKMSPI VRKIFLQFM PKLLIMRRT TYTLPEYDD 
      SLPPRGYSG EIEMQLNMR DPFNTDFKM KAHSSMSSS 
      NITPRFLSA NVLSALQGV RFIAQHIRD ADKDNEIVE 
      DWKFVSMVL DRFFLWVFT LACIGGTFG IILQAPSLY 
      DTRVPVHQQ LSEIPLTKI FQIPPSRTH IPTQ
    CDS sequence
    • >TC004836 RA
      atgctgttt aaatccact gaagcctcc aaacacatc 
      gaggctaat cctcatgct aagcgatta tacgacgac 
      ctcctcagt aattacaat cggctgatc agacctgtg 
      gaaaaccat acgcaaact ctaactgta tggctgggc 
      ctaaaactg tcacaattg atcgaaatg aacctaaaa 
      aatcaagtc atgacgacc aatttatgg gtggtgcag 
      agatggtac gactataaa ttaaagtgg gaaccggag 
      gaatatggc ggtgtggaa atgctttac gtcccttct 
      gagcatatt tggcttccg gacatcgtt ttattcaat 
      aatgccgat ggtaattat gaggtgact ttgatgact 
      aaagccact ttgtcttac accggcgaa gtaatttgg 
      aagccacct tcgatctat aaatcttct tgcgaaata 
      aacgtgcaa tattttccc ttcgacgag caaagttgt 
      ctaatgaaa ttcggttct tggacttac aatggcctt 
      caagtcgac ttgaagcac atggaccaa gtctccggc 
      aacaatatt gtcaaagtc ggtattgac ttgtccgaa 
      ttttacctt tccgtcgaa tgggacatc cttgcagtc 
      cccgccacg cgaaacgaa gagtactat ccttgttgc 
      acagagccg tattcagat atcactttc aagataaca 
      atgcgtcgc aagacatta ttctataca gtcaacttg 
      atcattcct tgcgtcggg atcacattt cttacagtt 
      cttgttttc taccttcct tcggattcg ggcgagaaa 
      gtcacactt tgtgtctca atcctatta tctctaact 
      gtgtttttc ctgttgctc gctgaaatt attccgccg 
      acttcgttg gccgtccct ttgttgggc aaatacctc 
      ctctttacc atgattttg gtcacttcg tccatatgg 
      gtcacggtg tgcgtgctc aacgtgcat ttcagatcc 
      ccttcgact cacaaaatg tcgccgatt gtgcgaaaa 
      atcttcctc caattcatg cccaaactg ctgattatg 
      cggcggacg acttacact ttgcctgag tatgacgac 
      agtttgcct cccagagga tacagtggc gagatcgaa 
      atgcaattg aatatgaga gatccattc aacactgat 
      ttcaaaatg aaagcacat tcgtcgatg tcctcctcg 
      aatataact cctcgtttc ctgtcggcg aacgttctc 
      tcggctctg cagggtgtt aggtttatt gcacaacac 
      atcagagat gccgataaa gataatgaa atcgttgaa 
      gattggaag tttgtttcg atggtccta gatcgattc 
      tttttatgg gtcttcact ctggcttgc atcggcgga 
      actttcggg attatatta caagctcct tccttgtac 
      gacacccga gtgcctgtt catcaacaa cttagcgaa 
      attcctctc acgaaaatt ttccaaata cccccctcg 
      cgcacacac atccccaca caataa
    mRNA sequence
    • >TC004836 RA
      cctggcgat gtgggagta aaacaacat ttccttaat 
      tagcctagt tatgtattt aataaaatt agtttgttt 
      cactttcat gctgtttaa atccactga agcctccaa 
      acacatcga ggctaatcc tcatgctaa gcgattata 
      cgacgacct cctcagtaa ttacaatcg gctgatcag 
      acctgtgga aaaccatac gcaaactct aactgtatg 
      gctgggcct aaaactgtc acaattgat cgaaatgaa 
      cctaaaaaa tcaagtcat gacgaccaa tttatgggt 
      ggtgcagag atggtacga ctataaatt aaagtggga 
      accggagga atatggcgg tgtggaaat gctttacgt 
      cccttctga gcatatttg gcttccgga catcgtttt 
      attcaataa tgccgatgg taattatga ggtgacttt 
      gatgactaa agccacttt gtcttacac cggcgaagt 
      aatttggaa gccaccttc gatctataa atcttcttg 
      cgaaataaa cgtgcaata ttttccctt cgacgagca 
      aagttgtct aatgaaatt cggttcttg gacttacaa 
      tggccttca agtcgactt gaagcacat ggaccaagt 
      ctccggcaa caatattgt caaagtcgg tattgactt 
      gtccgaatt ttacctttc cgtcgaatg ggacatcct 
      tgcagtccc cgccacgcg aaacgaaga gtactatcc 
      ttgttgcac agagccgta ttcagatat cactttcaa 
      gataacaat gcgtcgcaa gacattatt ctatacagt 
      caacttgat cattccttg cgtcgggat cacatttct 
      tacagttct tgttttcta ccttccttc ggattcggg 
      cgagaaagt cacactttg tgtctcaat cctattatc 
      tctaactgt gtttttcct gttgctcgc tgaaattat 
      tccgccgac ttcgttggc cgtcccttt gttgggcaa 
      atacctcct ctttaccat gattttggt cacttcgtc 
      catatgggt cacggtgtg cgtgctcaa cgtgcattt 
      cagatcccc ttcgactca caaaatgtc gccgattgt 
      gcgaaaaat cttcctcca attcatgcc caaactgct 
      gattatgcg gcggacgac ttacacttt gcctgagta 
      tgacgacag tttgcctcc cagaggata cagtggcga 
      gatcgaaat gcaattgaa tatgagaga tccattcaa 
      cactgattt caaaatgaa agcacattc gtcgatgtc 
      ctcctcgaa tataactcc tcgtttcct gtcggcgaa 
      cgttctctc ggctctgca gggtgttag gtttattgc 
      acaacacat cagagatgc cgataaaga taatgaaat 
      cgttgaaga ttggaagtt tgtttcgat ggtcctaga 
      tcgattctt tttatgggt cttcactct ggcttgcat 
      cggcggaac tttcgggat tatattaca agctccttc 
      cttgtacga cacccgagt gcctgttca tcaacaact 
      tagcgaaat tcctctcac gaaaatttt ccaaatacc 
      cccctcgcg cacacacat ccccacaca ataaaccac 
      agggtataa ccaaaaaac cttcttcct ctaaatggg 
      accattgca caattctaa caaagcaca actactttt 
      tgaatgttt tatggggca attttccaa aaatcgcac 
      aatttttta cccaaacac ttcacactt ttgtggacc 
      tagcctctg ttaccaaat gttatatgt gcttaaaat 
      acaataaaa accgttaga acat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY