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Gene information
General information
TC004830 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2518394..2536534
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004830
    iBeetle screen iB_03854

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004830 RA
      MVGRTEYSS VPTAVDLEN NTNQKKYTV NREPGGLVI 
      PKSLCALMA IGALLLAIL VGLLVFFFV VPRCSQASD 
      TRPSDLRVG DDGLPSISD EISLTKIDS PSLELDERL 
      PRSLEPTHY RIQVRPFFS NLTFDGTVT ITMHVKEQT 
      DQIIFNVKD IEIDKQSVK VRSVKSNTP LGISRQDYV 
      PGERYKIVL DSSLDKNIM YTLELTYVG HLNNHLQGF 
      YRSQYDENN SVKYLASTQ FSPTDARRA FPCFDEPSF 
      KANFSLIVG RPSNMSSLA NMPLIKSDS DWDYYETTP 
      KMSPYLVAF VVSNLQAYG SSDKLIKVW TRETLRIQA 
      RYAAEFAPK VLHYFENYF NIAFPLPKI DIVAIPDFG 
      YNAMENWGL ITFRESSLL YNTDEPDVD TKRTIATIL 
      SHELGHQWF GNLVTPKWW NDLWLKEGF ATYLQYLGA 
      DFAEPSWNI KEEFIFSET ARAFALDAL QSSRPISYE 
      VKNSRQIRQ TFDELSYAK GASVVRMMN NFLGEDAFK 
      TGLINYLRK YEYSNGDRD DLFGALTEV AHRKGALEP 
      SVTVKDVMD SWTKQPGFP VITAIRDPA NKKLILSQK 
      RFLFTDNHN DSSTWWVPV SVTTNGGNF ETQPTVWLK 
      NEPMVTINL NTSLWYLIN INQTGYYIV NYDEANWRS 
      LTRHLMSLP TIIRAQLIS DSMDLARAN LLDYDIPLK 
      LVQHMALRD KFIMFVPTN VAFKKLEFL SDMLSATPA 
      FGLFESFVS TIFRDTYRT VNSDLDYGS ENYLHQKIR 
      KMVLKWACR KTDSECAIT SHRMFRDWM EYARTIPPN 
      IRDVVYCTA IRQGSEAEW RFAFNRYLN TSSVSEKNV 
      LLDALGCTT QKWLLSRYL DNLVRNHSS IRIQDADRV 
      FKSVCDNNI ASTLAFDFL RTNWDKLIS FYGEGFNII 
      SKMVKSLPR FMNTEYQLS ELVRFRNQV RHNLGTASQ 
      AFDSAIERV RGNVAWMKK NYHNVEKWL IDNKEHFHA
    • >TC004830 RB
      MVGHYNHGV PTAVDLENN TNQKKYTVN REPGGLVIP 
      KSLCALMAI GALLLAILV GLLVFFFVV PRCSQASDT 
      RPSDLRVGD DGLPSISDE ISLTKIDSP SLELDERLP 
      RSLEPTHYR IQVRPFFSN LTFDGTVTI TMHVKEQTD 
      QIIFNVKDI EIDKQSVKV RSVKSNTPL GISRQDYVP 
      GERYKIVLD SSLDKNIMY TLELTYVGH LNNHLQGFY 
      RSQYDENNS VKYLASTQF SPTDARRAF PCFDEPSFK 
      ANFSLIVGR PSNMSSLAN MPLIKSDSD WDYYETTPK 
      MSPYLVAFV VSNLQAYGS SDKLIKVWT RETLRIQAR 
      YAAEFAPKV LHYFENYFN IAFPLPKID IVAIPDFGY 
      NAMENWGLI TFRESSLLY NTDEPDVDT KRTIATILS 
      HELGHQWFG NLVTPKWWN DLWLKEGFA TYLQYLGAD 
      FAEPSWNIK EEFIFSETA RAFALDALQ SSRPISYEV 
      KNSRQIRQT FDELSYAKG ASVVRMMNN FLGEDAFKT 
      GLINYLRKY EYSNGDRDD LFGALTEVA HRKGALEPS 
      VTVKDVMDS WTKQPGFPV ITAIRDPAN KKLILSQKR 
      FLFTDNHND SSTWWVPVS VTTNGGNFE TQPTVWLKN 
      EPMVTINLN TSLWYLINI NQTGYYIVN YDEANWRSL 
      TRHLMSLPT IIRAQLISD SMDLARANL LDYDIPLKL 
      VQHMALRDK FIMFVPTNV AFKKLEFLS DMLSATPAF 
      GLFESFVST IFRDTYRTV NSDLDYGSE NYLHQKIRK 
      MVLKWACRK TDSECAITS HRMFRDWME YARTIPPNI 
      RDVVYCTAI RQGSEAEWR FAFNRYLNT SSVSEKNVL 
      LDALGCTTQ KWLLSRYLD NLVRNHSSI RIQDADRVF 
      KSVCDNNIA STLAFDFLR TNWDKLISF YGEGFNIIS 
      KMVKSLPRF MNTEYQLSE LVRFRNQVR HNLGTASQA 
      FDSAIERVR GNVAWMKKN YHNVEKWLI DNKEHFHA
    CDS sequence
    • >TC004830 RA
      atggtgggc cggactgag tactccagt gtgcccact 
      gcggtggac ttggagaac aacaccaac caaaagaag 
      tacaccgta aatcgagaa cctggcggt ttagttata 
      ccaaaatcc ctttgcgcc ctaatggcg ataggagcc 
      cttctatta gcaatactt gtaggttta ttagtcttt 
      ttcttcgtc gttcctaga tgtagtcaa gcttctgat 
      actcgacct agtgactta agggtgggc gatgacggc 
      ctcccaagc atatctgac gaaataagc ttaacaaaa 
      attgatagt cccagcttg gaacttgac gagaggctt 
      cccaggagt ttagaaccg acgcattat agaatccaa 
      gtacgtcct tttttctcc aatctgacg ttcgatggc 
      actgtcaca ataaccatg cacgtgaaa gagcaaacc 
      gatcaaatc attttcaac gtgaaagac atcgaaatt 
      gataaacaa tcagtgaaa gtgcgctcg gttaaaagc 
      aacactccg ttaggcatc tcaaggcag gattacgtc 
      cctggggag cgatacaaa atagttttg gactcgtct 
      ctggataaa aatattatg tacactcta gagttaaca 
      tacgtcggg catttaaac aaccatttg caaggattt 
      tatcggagc caatacgat gaaaataat agtgtcaaa 
      tatttggcg agcacccaa ttttccccg acagatgcc 
      cggcgtgct ttcccttgc ttcgatgag ccttcattc 
      aaggcaaat ttttcgtta attgttggg agaccgtcg 
      aatatgtct tccttggca aacatgcct ttgatcaaa 
      tccgactct gattgggat tattacgag acaacaccg 
      aaaatgtcc ccttacttg gtcgctttc gtcgtcagt 
      aatttacaa gcttatggc tcttcggat aagttaatc 
      aaagtctgg accagggaa actctgagg atccaagca 
      agatatgca gcagagttt gcgccaaaa gttttgcac 
      tatttcgaa aattacttc aacattgcg tttcctttg 
      cccaaaatt gatatcgtg gcgattccc gattttggg 
      tacaatgcg atggaaaat tggggtttg atcactttc 
      agggagagt tctctcttg tacaatacc gatgagcct 
      gatgttgac accaagagg accattgcc acgatttta 
      tcgcacgaa ttgggacat cagtggttt ggtaatctg 
      gtcaccccc aagtggtgg aacgatttg tggttgaag 
      gagggtttt gccacctat ttgcaatac ctaggagct 
      gattttgcc gaaccttct tggaacatc aaagaagag 
      tttattttc agtgaaaca gctcgtgct tttgctctc 
      gatgcgtta caatcctca cgacccatt tcctacgaa 
      gtgaagaac agtcgtcaa attcgacag actttcgac 
      gagctgtcg tacgccaaa ggggcctcg gtggtccga 
      atgatgaac aattttctg ggcgaagat gcgttcaaa 
      actggactt atcaactat ttacgaaag tacgaatac 
      agcaatggg gaccgtgac gatcttttt ggggccttg 
      acggaagtt gcccaccga aaaggggcc ctagaacca 
      tcagttact gtcaaagat gtgatggac tcatggacc 
      aaacagcca gggtttccc gttatcaca gctatcagg 
      gacccagca aacaaaaaa ctaattttg tctcagaaa 
      agattttta tttacggat aaccacaac gatagttcc 
      acttggtgg gtcccagtt tcagttaca acaaatggt 
      ggtaatttt gaaacgcag cctacagtg tggctcaaa 
      aatgagccc atggtcaca attaacctc aacacttcg 
      ttgtggtac ctcatcaac attaaccaa acaggctat 
      tacatcgta aactacgac gaggctaat tggagatcg 
      cttacacgg cacctaatg tctctcccg accataatt 
      agggcgcaa ttaattagc gattcaatg gacctagcc 
      cgcgcaaat ttgctagat tatgacatt cccttgaaa 
      ctagtccaa catatggcc ctacgtgac aaattcatt 
      atgtttgtt ccgacaaac gtggcgttc aaaaaactg 
      gaatttttg agtgatatg ttatccgca actccagct 
      tttggccta tttgaatcg ttcgtttcg acgatattt 
      agagacact tatcggaca gtaaactcc gacttggac 
      tacggttct gaaaactat ctccaccag aaaattcgc 
      aaaatggtt ttgaagtgg gcttgccgt aaaactgat 
      tcggagtgt gccattaca tcgcatcgt atgttcagg 
      gattggatg gaatatgca agaacaatc ccgccgaat 
      attcgtgat gttgtttat tgtacggcg atcagacaa 
      gggagcgag gccgaatgg agatttgct ttcaaccga 
      tacttgaac acgtcttca gtgagcgaa aagaacgtc 
      cttttggac gctttgggc tgcaccacc cagaaatgg 
      ctactttca aggtatctg gataatttg gtccgcaat 
      cattccagt atacgtatt caagacgca gacagagtt 
      ttcaaatcg gtgtgtgac aacaatatt gccagcact 
      ttagctttc gatttcctg agaacaaat tgggacaaa 
      ttgatatca ttctacggc gaaggtttc aatattatt 
      agtaaaatg gtgaagagt ttgccgcgg tttatgaac 
      actgaatat caactttct gagcttgta aggtttagg 
      aaccaagtc aggcacaat ttggggact gcaagtcag 
      gcctttgat agtgccatt gagagagta agaggaaac 
      gtggcttgg atgaagaag aattaccac aatgttgaa 
      aagtggttg attgataac aaagaacat ttccatgcg 
      taa
    • >TC004830 RB
      atggtcgga cactataat cacggtgtg cccactgcg 
      gtggacttg gagaacaac accaaccaa aagaagtac 
      accgtaaat cgagaacct ggcggttta gttatacca 
      aaatccctt tgcgcccta atggcgata ggagccctt 
      ctattagca atacttgta ggtttatta gtctttttc 
      ttcgtcgtt cctagatgt agtcaagct tctgatact 
      cgacctagt gacttaagg gtgggcgat gacggcctc 
      ccaagcata tctgacgaa ataagctta acaaaaatt 
      gatagtccc agcttggaa cttgacgag aggcttccc 
      aggagttta gaaccgacg cattataga atccaagta 
      cgtcctttt ttctccaat ctgacgttc gatggcact 
      gtcacaata accatgcac gtgaaagag caaaccgat 
      caaatcatt ttcaacgtg aaagacatc gaaattgat 
      aaacaatca gtgaaagtg cgctcggtt aaaagcaac 
      actccgtta ggcatctca aggcaggat tacgtccct 
      ggggagcga tacaaaata gttttggac tcgtctctg 
      gataaaaat attatgtac actctagag ttaacatac 
      gtcgggcat ttaaacaac catttgcaa ggattttat 
      cggagccaa tacgatgaa aataatagt gtcaaatat 
      ttggcgagc acccaattt tccccgaca gatgcccgg 
      cgtgctttc ccttgcttc gatgagcct tcattcaag 
      gcaaatttt tcgttaatt gttgggaga ccgtcgaat 
      atgtcttcc ttggcaaac atgcctttg atcaaatcc 
      gactctgat tgggattat tacgagaca acaccgaaa 
      atgtcccct tacttggtc gctttcgtc gtcagtaat 
      ttacaagct tatggctct tcggataag ttaatcaaa 
      gtctggacc agggaaact ctgaggatc caagcaaga 
      tatgcagca gagtttgcg ccaaaagtt ttgcactat 
      ttcgaaaat tacttcaac attgcgttt cctttgccc 
      aaaattgat atcgtggcg attcccgat tttgggtac 
      aatgcgatg gaaaattgg ggtttgatc actttcagg 
      gagagttct ctcttgtac aataccgat gagcctgat 
      gttgacacc aagaggacc attgccacg attttatcg 
      cacgaattg ggacatcag tggtttggt aatctggtc 
      acccccaag tggtggaac gatttgtgg ttgaaggag 
      ggttttgcc acctatttg caataccta ggagctgat 
      tttgccgaa ccttcttgg aacatcaaa gaagagttt 
      attttcagt gaaacagct cgtgctttt gctctcgat 
      gcgttacaa tcctcacga cccatttcc tacgaagtg 
      aagaacagt cgtcaaatt cgacagact ttcgacgag 
      ctgtcgtac gccaaaggg gcctcggtg gtccgaatg 
      atgaacaat tttctgggc gaagatgcg ttcaaaact 
      ggacttatc aactattta cgaaagtac gaatacagc 
      aatggggac cgtgacgat ctttttggg gccttgacg 
      gaagttgcc caccgaaaa ggggcccta gaaccatca 
      gttactgtc aaagatgtg atggactca tggaccaaa 
      cagccaggg tttcccgtt atcacagct atcagggac 
      ccagcaaac aaaaaacta attttgtct cagaaaaga 
      tttttattt acggataac cacaacgat agttccact 
      tggtgggtc ccagtttca gttacaaca aatggtggt 
      aattttgaa acgcagcct acagtgtgg ctcaaaaat 
      gagcccatg gtcacaatt aacctcaac acttcgttg 
      tggtacctc atcaacatt aaccaaaca ggctattac 
      atcgtaaac tacgacgag gctaattgg agatcgctt 
      acacggcac ctaatgtct ctcccgacc ataattagg 
      gcgcaatta attagcgat tcaatggac ctagcccgc 
      gcaaatttg ctagattat gacattccc ttgaaacta 
      gtccaacat atggcccta cgtgacaaa ttcattatg 
      tttgttccg acaaacgtg gcgttcaaa aaactggaa 
      tttttgagt gatatgtta tccgcaact ccagctttt 
      ggcctattt gaatcgttc gtttcgacg atatttaga 
      gacacttat cggacagta aactccgac ttggactac 
      ggttctgaa aactatctc caccagaaa attcgcaaa 
      atggttttg aagtgggct tgccgtaaa actgattcg 
      gagtgtgcc attacatcg catcgtatg ttcagggat 
      tggatggaa tatgcaaga acaatcccg ccgaatatt 
      cgtgatgtt gtttattgt acggcgatc agacaaggg 
      agcgaggcc gaatggaga tttgctttc aaccgatac 
      ttgaacacg tcttcagtg agcgaaaag aacgtcctt 
      ttggacgct ttgggctgc accacccag aaatggcta 
      ctttcaagg tatctggat aatttggtc cgcaatcat 
      tccagtata cgtattcaa gacgcagac agagttttc 
      aaatcggtg tgtgacaac aatattgcc agcacttta 
      gctttcgat ttcctgaga acaaattgg gacaaattg 
      atatcattc tacggcgaa ggtttcaat attattagt 
      aaaatggtg aagagtttg ccgcggttt atgaacact 
      gaatatcaa ctttctgag cttgtaagg tttaggaac 
      caagtcagg cacaatttg gggactgca agtcaggcc 
      tttgatagt gccattgag agagtaaga ggaaacgtg 
      gcttggatg aagaagaat taccacaat gttgaaaag 
      tggttgatt gataacaaa gaacatttc catgcgtaa
    mRNA sequence
    • >TC004830 RA
      tgagtgaaa cacatgtgc tcaaacgaa taatttaca 
      tttgttgtg taaataact agaactgtc gagatagtt 
      ttgctgtca caaaaggtg aatgaacaa cgtgtgggc 
      aaaccggcc gttttaaaa tgttgctcc atgaggtgc 
      actgtttgg agtagtgaa tcaatttga atgaaatta 
      ctcatctgt acgcaacgt gctaataaa tgaggtaaa 
      tgtgtggtt ttgcatcac atcatcgga tgcaaagaa 
      ctggtgaaa ctctggtgt tgtggtggt ttatagacg 
      ctcagagac tttttttgt aatttcaca aaatggtgg 
      gccggactg agtactcca gtgtgccca ctgcggtgg 
      acttggaga acaacacca accaaaaga agtacaccg 
      taaatcgag aacctggcg gtttagtta taccaaaat 
      ccctttgcg ccctaatgg cgataggag cccttctat 
      tagcaatac ttgtaggtt tattagtct ttttcttcg 
      tcgttccta gatgtagtc aagcttctg atactcgac 
      ctagtgact taagggtgg gcgatgacg gcctcccaa 
      gcatatctg acgaaataa gcttaacaa aaattgata 
      gtcccagct tggaacttg acgagaggc ttcccagga 
      gtttagaac cgacgcatt atagaatcc aagtacgtc 
      cttttttct ccaatctga cgttcgatg gcactgtca 
      caataacca tgcacgtga aagagcaaa ccgatcaaa 
      tcattttca acgtgaaag acatcgaaa ttgataaac 
      aatcagtga aagtgcgct cggttaaaa gcaacactc 
      cgttaggca tctcaaggc aggattacg tccctgggg 
      agcgataca aaatagttt tggactcgt ctctggata 
      aaaatatta tgtacactc tagagttaa catacgtcg 
      ggcatttaa acaaccatt tgcaaggat tttatcgga 
      gccaatacg atgaaaata atagtgtca aatatttgg 
      cgagcaccc aattttccc cgacagatg cccggcgtg 
      ctttccctt gcttcgatg agccttcat tcaaggcaa 
      atttttcgt taattgttg ggagaccgt cgaatatgt 
      cttccttgg caaacatgc ctttgatca aatccgact 
      ctgattggg attattacg agacaacac cgaaaatgt 
      ccccttact tggtcgctt tcgtcgtca gtaatttac 
      aagcttatg gctcttcgg ataagttaa tcaaagtct 
      ggaccaggg aaactctga ggatccaag caagatatg 
      cagcagagt ttgcgccaa aagttttgc actatttcg 
      aaaattact tcaacattg cgtttcctt tgcccaaaa 
      ttgatatcg tggcgattc ccgattttg ggtacaatg 
      cgatggaaa attggggtt tgatcactt tcagggaga 
      gttctctct tgtacaata ccgatgagc ctgatgttg 
      acaccaaga ggaccattg ccacgattt tatcgcacg 
      aattgggac atcagtggt ttggtaatc tggtcaccc 
      ccaagtggt ggaacgatt tgtggttga aggagggtt 
      ttgccacct atttgcaat acctaggag ctgattttg 
      ccgaacctt cttggaaca tcaaagaag agtttattt 
      tcagtgaaa cagctcgtg cttttgctc tcgatgcgt 
      tacaatcct cacgaccca tttcctacg aagtgaaga 
      acagtcgtc aaattcgac agactttcg acgagctgt 
      cgtacgcca aaggggcct cggtggtcc gaatgatga 
      acaattttc tgggcgaag atgcgttca aaactggac 
      ttatcaact atttacgaa agtacgaat acagcaatg 
      gggaccgtg acgatcttt ttggggcct tgacggaag 
      ttgcccacc gaaaagggg ccctagaac catcagtta 
      ctgtcaaag atgtgatgg actcatgga ccaaacagc 
      cagggtttc ccgttatca cagctatca gggacccag 
      caaacaaaa aactaattt tgtctcaga aaagatttt 
      tatttacgg ataaccaca acgatagtt ccacttggt 
      gggtcccag tttcagtta caacaaatg gtggtaatt 
      ttgaaacgc agcctacag tgtggctca aaaatgagc 
      ccatggtca caattaacc tcaacactt cgttgtggt 
      acctcatca acattaacc aaacaggct attacatcg 
      taaactacg acgaggcta attggagat cgcttacac 
      ggcacctaa tgtctctcc cgaccataa ttagggcgc 
      aattaatta gcgattcaa tggacctag cccgcgcaa 
      atttgctag attatgaca ttcccttga aactagtcc 
      aacatatgg ccctacgtg acaaattca ttatgtttg 
      ttccgacaa acgtggcgt tcaaaaaac tggaatttt 
      tgagtgata tgttatccg caactccag cttttggcc 
      tatttgaat cgttcgttt cgacgatat ttagagaca 
      cttatcgga cagtaaact ccgacttgg actacggtt 
      ctgaaaact atctccacc agaaaattc gcaaaatgg 
      ttttgaagt gggcttgcc gtaaaactg attcggagt 
      gtgccatta catcgcatc gtatgttca gggattgga 
      tggaatatg caagaacaa tcccgccga atattcgtg 
      atgttgttt attgtacgg cgatcagac aagggagcg 
      aggccgaat ggagatttg ctttcaacc gatacttga 
      acacgtctt cagtgagcg aaaagaacg tccttttgg 
      acgctttgg gctgcacca cccagaaat ggctacttt 
      caaggtatc tggataatt tggtccgca atcattcca 
      gtatacgta ttcaagacg cagacagag ttttcaaat 
      cggtgtgtg acaacaata ttgccagca ctttagctt 
      tcgatttcc tgagaacaa attgggaca aattgatat 
      cattctacg gcgaaggtt tcaatatta ttagtaaaa 
      tggtgaaga gtttgccgc ggtttatga acactgaat 
      atcaacttt ctgagcttg taaggttta ggaaccaag 
      tcaggcaca atttgggga ctgcaagtc aggcctttg 
      atagtgcca ttgagagag taagaggaa acgtggctt 
      ggatgaaga agaattacc acaatgttg aaaagtggt 
      tgattgata acaaagaac atttccatg cgtaattaa 
      ttaactaga ctagacaaa ttagtattt taactttat 
      tgcattaac tgtttgaca tccaaagaa tgtaaagtg 
      atagtgtaa ctgtacctt cttgttaat gttttattg 
      tgctttttc tactctatt ttatcaggt cgagacgat 
      ttcataatt tatttttaa tagaaacat taataaaca 
      ataatacca ggt
    • >TC004830 RB
      tgagtgaaa cacatgtgc tcaaacgaa taatttaca 
      tttgttgtg taaataact agaactgtc gagatagtt 
      ttgctgtca caaaaggtg aatgaacaa cgtgtgggc 
      aaaccggcc gttttaaaa tgttgctcc atgaggtgc 
      actgtttgg agtagtgaa tcaatttga atgaaatta 
      ctcatctgt acgcaacgt gctaataaa tgaggtaaa 
      tgtgtggtt ttgcatcac atcatcgga tgcaaagaa 
      ctggtgaaa ctctggtgt tgtggtggt ttatagacg 
      ctcagagac tttttttgt aatttcaca aaatggtgg 
      gccggactg agtactcca cttacctca gcgcgctgc 
      gcattgcca acctgtaaa caaagttta aaataaaat 
      tgacgacaa atacaataa tttttgatt tgacgaaat 
      ctgcgcgag ttcggactt ccggcgtcg ggatcggcg 
      tcagtgcac cgggtggac gtcgtcagt ccaacgtcg 
      tcgagagtg aaatgtgct aattagtgt gggacaaga 
      aaaacatgg tcggacact ataatcacg gtgtgccca 
      ctgcggtgg acttggaga acaacacca accaaaaga 
      agtacaccg taaatcgag aacctggcg gtttagtta 
      taccaaaat ccctttgcg ccctaatgg cgataggag 
      cccttctat tagcaatac ttgtaggtt tattagtct 
      ttttcttcg tcgttccta gatgtagtc aagcttctg 
      atactcgac ctagtgact taagggtgg gcgatgacg 
      gcctcccaa gcatatctg acgaaataa gcttaacaa 
      aaattgata gtcccagct tggaacttg acgagaggc 
      ttcccagga gtttagaac cgacgcatt atagaatcc 
      aagtacgtc cttttttct ccaatctga cgttcgatg 
      gcactgtca caataacca tgcacgtga aagagcaaa 
      ccgatcaaa tcattttca acgtgaaag acatcgaaa 
      ttgataaac aatcagtga aagtgcgct cggttaaaa 
      gcaacactc cgttaggca tctcaaggc aggattacg 
      tccctgggg agcgataca aaatagttt tggactcgt 
      ctctggata aaaatatta tgtacactc tagagttaa 
      catacgtcg ggcatttaa acaaccatt tgcaaggat 
      tttatcgga gccaatacg atgaaaata atagtgtca 
      aatatttgg cgagcaccc aattttccc cgacagatg 
      cccggcgtg ctttccctt gcttcgatg agccttcat 
      tcaaggcaa atttttcgt taattgttg ggagaccgt 
      cgaatatgt cttccttgg caaacatgc ctttgatca 
      aatccgact ctgattggg attattacg agacaacac 
      cgaaaatgt ccccttact tggtcgctt tcgtcgtca 
      gtaatttac aagcttatg gctcttcgg ataagttaa 
      tcaaagtct ggaccaggg aaactctga ggatccaag 
      caagatatg cagcagagt ttgcgccaa aagttttgc 
      actatttcg aaaattact tcaacattg cgtttcctt 
      tgcccaaaa ttgatatcg tggcgattc ccgattttg 
      ggtacaatg cgatggaaa attggggtt tgatcactt 
      tcagggaga gttctctct tgtacaata ccgatgagc 
      ctgatgttg acaccaaga ggaccattg ccacgattt 
      tatcgcacg aattgggac atcagtggt ttggtaatc 
      tggtcaccc ccaagtggt ggaacgatt tgtggttga 
      aggagggtt ttgccacct atttgcaat acctaggag 
      ctgattttg ccgaacctt cttggaaca tcaaagaag 
      agtttattt tcagtgaaa cagctcgtg cttttgctc 
      tcgatgcgt tacaatcct cacgaccca tttcctacg 
      aagtgaaga acagtcgtc aaattcgac agactttcg 
      acgagctgt cgtacgcca aaggggcct cggtggtcc 
      gaatgatga acaattttc tgggcgaag atgcgttca 
      aaactggac ttatcaact atttacgaa agtacgaat 
      acagcaatg gggaccgtg acgatcttt ttggggcct 
      tgacggaag ttgcccacc gaaaagggg ccctagaac 
      catcagtta ctgtcaaag atgtgatgg actcatgga 
      ccaaacagc cagggtttc ccgttatca cagctatca 
      gggacccag caaacaaaa aactaattt tgtctcaga 
      aaagatttt tatttacgg ataaccaca acgatagtt 
      ccacttggt gggtcccag tttcagtta caacaaatg 
      gtggtaatt ttgaaacgc agcctacag tgtggctca 
      aaaatgagc ccatggtca caattaacc tcaacactt 
      cgttgtggt acctcatca acattaacc aaacaggct 
      attacatcg taaactacg acgaggcta attggagat 
      cgcttacac ggcacctaa tgtctctcc cgaccataa 
      ttagggcgc aattaatta gcgattcaa tggacctag 
      cccgcgcaa atttgctag attatgaca ttcccttga 
      aactagtcc aacatatgg ccctacgtg acaaattca 
      ttatgtttg ttccgacaa acgtggcgt tcaaaaaac 
      tggaatttt tgagtgata tgttatccg caactccag 
      cttttggcc tatttgaat cgttcgttt cgacgatat 
      ttagagaca cttatcgga cagtaaact ccgacttgg 
      actacggtt ctgaaaact atctccacc agaaaattc 
      gcaaaatgg ttttgaagt gggcttgcc gtaaaactg 
      attcggagt gtgccatta catcgcatc gtatgttca 
      gggattgga tggaatatg caagaacaa tcccgccga 
      atattcgtg atgttgttt attgtacgg cgatcagac 
      aagggagcg aggccgaat ggagatttg ctttcaacc 
      gatacttga acacgtctt cagtgagcg aaaagaacg 
      tccttttgg acgctttgg gctgcacca cccagaaat 
      ggctacttt caaggtatc tggataatt tggtccgca 
      atcattcca gtatacgta ttcaagacg cagacagag 
      ttttcaaat cggtgtgtg acaacaata ttgccagca 
      ctttagctt tcgatttcc tgagaacaa attgggaca 
      aattgatat cattctacg gcgaaggtt tcaatatta 
      ttagtaaaa tggtgaaga gtttgccgc ggtttatga 
      acactgaat atcaacttt ctgagcttg taaggttta 
      ggaaccaag tcaggcaca atttgggga ctgcaagtc 
      aggcctttg atagtgcca ttgagagag taagaggaa 
      acgtggctt ggatgaaga agaattacc acaatgttg 
      aaaagtggt tgattgata acaaagaac atttccatg 
      cgtaattaa ttaactaga ctagacaaa ttagtattt 
      taactttat tgcattaac tgtttgaca tccaaagaa 
      tgtaaagtg atagtgtaa ctgtacctt cttgttaat 
      gttttattg tgctttttc tactctatt ttatcaggt 
      cgagacgat ttcataatt tatttttaa tagaaacat 
      taataaaca ataatacca ggt
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