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Gene information
General information
TC004829 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 418381..419806
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004829
    iBeetle screen iB_03853
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004829 RA
      MLQAIRKLI PFYAPSPEL PSLPIKMSE VKIVRKPRY 
      DGRVKKRQW EERRSDKGE GLNFHKSKI IKKETEVTG 
      ELIEKIKRR KFAVLLGYS GTNYYGMQR NPNTPTIEE 
      EFFKALYKN NYISEENFT QVQTMQFQR AARTDKGVS 
      AARQVVSLK LREDIDISK INEQLPEVI RVFGIRRVT 
      KGFNSKVQC DSRSYTYLL PTVSFTPHD QLMVQKGFR 
      LDDSTLNRI NDLLQKYLG TKNFHNFTS KKQATDPSA 
      KRYMKTLIC EKPFVKDDV EFALIKIRG QSFMLHQIR 
      KMVGLLIAV VKGYTSEDT LDLAFGMDK VNIPKAPGL 
      GLLLDYVHY ERYDNRYGA DGVHEKLVW DDVEKEVEE 
      FREKYIFPT IINTEVKDE AMVAWIIRR LSTHKYDDI 
      DDEDDKSDS EQTVNSDNR
    CDS sequence
    • >TC004829 RA
      atgttgcaa gccataagg aaattaatt cctttttac 
      gccccctcg cccgaattg ccgtctcta ccgataaaa 
      atgagcgag gtgaagatt gtccggaag ccccgttac 
      gacggtcgt gtcaaaaaa cggcaatgg gaggaacga 
      agaagtgac aagggtgaa ggtttaaac ttccacaag 
      tcgaaaata attaaaaaa gaaacggaa gtaacggga 
      gaattgatc gaaaaaatc aaacgtcgc aaattcgcc 
      gtgttgctg ggctattcg gggacgaat tactacgga 
      atgcaacga aaccccaac actccaaca atcgaagag 
      gagtttttc aaagcgctt tataaaaat aattacatc 
      agtgaggaa aatttcaca caagtgcag accatgcag 
      tttcaaagg gccgcaagg actgataaa ggcgtgtca 
      gcggcgcgt caagttgtt tcgttaaag ttacgcgaa 
      gacatcgat atttccaaa attaatgaa caactgccc 
      gaagtgatt cgagttttt ggcattaga agggtcaca 
      aaagggttc aatagtaaa gttcagtgc gacagtagg 
      tcctacact tacttgctc cccactgtg tcatttacc 
      cctcatgac caactaatg gtgcaaaaa gggttcaga 
      cttgatgac tccacctta aatcggatt aatgatctt 
      ttacaaaaa tacctgggc acgaaaaac ttccacaat 
      tttacgtcc aaaaaacaa gccactgat ccgagcgct 
      aaacggtac atgaagacc ctaatttgt gagaaaccc 
      ttcgttaaa gacgacgtt gaattcgcg ctcataaaa 
      ataaggggg caaagtttt atgttgcac caaatccgc 
      aaaatggtg gggttgctc attgcggtg gttaagggg 
      tacacatct gaagacact ttagacttg gcgtttggg 
      atggacaag gttaatata cctaaagcc cccggtttg 
      gggttactt ttggactac gtccattac gaacggtat 
      gataacaga tacggtgcg gatggggtc cacgaaaaa 
      ttggtttgg gacgatgtt gagaaggaa gtcgaggag 
      tttagggaa aaatatatt ttcccaact attattaat 
      acggaagtt aaggacgag gcaatggtg gcatggatt 
      ataaggagg ttatcgacg cataaatat gatgatatt 
      gacgatgag gatgacaag agtgatagt gaacaaact 
      gttaattcc gataataga tga
    mRNA sequence
    • >TC004829 RA
      ttggtttct gagtttgaa tttccgcgg caaaaaatt 
      cggcgttac cctcattct gtcacaaaa acttgacac 
      atcgaggtt atgtttacg tcacttttt tgaaaagag 
      gttatgttg caagccata aggaaatta attcctttt 
      tacgccccc tcgcccgaa ttgccgtct ctaccgata 
      aaaatgagc gaggtgaag attgtccgg aagccccgt 
      tacgacggt cgtgtcaaa aaacggcaa tgggaggaa 
      cgaagaagt gacaagggt gaaggttta aacttccac 
      aagtcgaaa ataattaaa aaagaaacg gaagtaacg 
      ggagaattg atcgaaaaa atcaaacgt cgcaaattc 
      gccgtgttg ctgggctat tcggggacg aattactac 
      ggaatgcaa cgaaacccc aacactcca acaatcgaa 
      gaggagttt ttcaaagcg ctttataaa aataattac 
      atcagtgag gaaaatttc acacaagtg cagaccatg 
      cagtttcaa agggccgca aggactgat aaaggcgtg 
      tcagcggcg cgtcaagtt gtttcgtta aagttacgc 
      gaagacatc gatatttcc aaaattaat gaacaactg 
      cccgaagtg attcgagtt tttggcatt agaagggtc 
      acaaaaggg ttcaatagt aaagttcag tgcgacagt 
      aggtcctac acttacttg ctccccact gtgtcattt 
      acccctcat gaccaacta atggtgcaa aaagggttc 
      agacttgat gactccacc ttaaatcgg attaatgat 
      cttttacaa aaatacctg ggcacgaaa aacttccac 
      aattttacg tccaaaaaa caagccact gatccgagc 
      gctaaacgg tacatgaag accctaatt tgtgagaaa 
      cccttcgtt aaagacgac gttgaattc gcgctcata 
      aaaataagg gggcaaagt tttatgttg caccaaatc 
      cgcaaaatg gtggggttg ctcattgcg gtggttaag 
      gggtacaca tctgaagac actttagac ttggcgttt 
      gggatggac aaggttaat atacctaaa gcccccggt 
      ttggggtta cttttggac tacgtccat tacgaacgg 
      tatgataac agatacggt gcggatggg gtccacgaa 
      aaattggtt tgggacgat gttgagaag gaagtcgag 
      gagtttagg gaaaaatat attttccca actattatt 
      aatacggaa gttaaggac gaggcaatg gtggcatgg 
      attataagg aggttatcg acgcataaa tatgatgat 
      attgacgat gaggatgac aagagtgat agtgaacaa 
      actgttaat tccgataat agatgataa tttttgtat 
      ttatttttt atcacttgt atgacatta gttaaagaa 
      acaataaat aattctaaa acat