| General information | |||
| TC004825 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2457739..2471259 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004825 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03852 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004825 RA
MASFMTKKK KYKFQVSLC LEELLEVPF VSAVLFAKI RLLDGGNFQ DHSSREEVR DHKVKWGAN FSFPCKMSA NASTGVLER CILRISVRK ECKGGRSFT KLGFVDLNL AEYAGAGVT FKKALLEGY DARHRQDNS MLKFSIAMH MLSGDILFK VPSPSLKHK SIASEDPAT ESRTDEFSS ESLGGSIAS GSSGFGSLP KKRPALLSS ELVIGHTLV ENSVPVTVA VEGPDQVQA PEHEEVVCE TGHSRNSSN TSQLSKASG YSSIHSHCH SRQSSSGDS GHIRALSHR QNRKQNGNP FLNSSNFIR VNTFPNRIR IPNTIIHED VLNSTLETL TPVKSPENG ETTRYYTPD GYHTQLSNV SGSSTDDYK TPDESLDMR TDLFSQKFT ELRKSSSTS LIELNAQPN ESDLLLDNN RLSADNAIK IKQTDGVLK SKSDFEIPR VSPIQKTRF RGGFFRSDN LPAFLTPIL KRKSAQSTP KNPDFTSLN SLRTGTAGT DQLGTQPRN PSSSSLVLS ETGSLDRAK AAYERRKKT QIQDSDTGV VSGRVENTR VNANRLIAE ILKDTNLEQ ADDSAETSG LQLFIAKDG TAALGNHEV KSQMSTGAQ VFKQVVMDD NR
- >TC004825 RB
MASFMTKKK KYKFQVSLC LEELLEVPF VSAVLFAKI RLLDGGNFQ DHSSREEVR DHKVKWGAN FSFPCKMSA NASTGVLER CILRISVRK ECKGGRSFT KLGFVDLNL AEYAGAGVT FKKALLEGY DARHRQDNS MLKFSIAMH MLSGDILFK VPSPSLKHK SIASEDPAT ESRTDEFSS ESLGGSIAS GSSGFGSLP KKRPALLSS ELVIGHTLV ENSVPVTVA VEGPDQVQA PEHEEVVCE TGHSRNSSN TSQLSKASG YSSIHSHCH SRQSSSGDS GHIRALSHR QNRKQNGNP FLNSSNFIR VNTFPNRIR IPNTIIHED VLNSTLETL TPVKSPENG ETTRYYTPD GYHTQLSNV SGSSTDDYK TPDESLDMR TDLFSQKFT ELRKSSSTS LIELNAQPN ESDLLLDNN RLSADNAIK IKQTDGVLK SKSDFEIPR VSPIQKTRF RGGFFRSDN LPAFLTPIL KRKSAQSTP KNPDFTSLN SLRTGTAGT DQLGTQPRG LPHRKLLEG ASGGKLAPL AISPDETPT TVSSDCSQN LTIRRPSVT TNPSSSSLV LSETGSLDR AKAAYERRK KTQIQDSDT GVVSGRVEN TRVNANRLI AEILKDTNL EQADDSAET SGLQLFIAK DGTAALGNH EVKSQMSTG AQVFKQVVM DDNR
- >TC004825 RA
atggcgtcc ttcatgacg aaaaagaag aagtacaaa tttcaagtg agtctatgt ttggaagaa ctgctggaa gtgcctttc gtttctgcc gtccttttt gccaagata agactccta gatggggga aattttcag gaccatagc agcagagag gaggtgcgc gaccacaaa gtgaaatgg ggcgcaaac ttttccttc ccttgtaaa atgtcagcg aacgcatcc accggcgtc ctcgagcgt tgtatttta cggatatcc gtgcgaaag gagtgcaaa ggcggccgt tcatttacc aaactaggt ttcgtcgac ttaaatcta gcagaatac gcaggggcg ggtgtcacg ttcaagaaa gctttatta gaaggatac gacgccaga catcgtcaa gacaacagc atgctcaag ttttccatt gcaatgcat atgttatcg ggtgatatt ttattcaaa gtaccgtct ccttccctc aaacataaa tctatagct tccgaagat ccagcaacc gaatctcga acggatgag ttttcgagt gagtcgctg ggtggttcc attgcaagt ggaagttcc ggatttggg agtttgccc aaaaaacga ccagcgctt ttatcgtca gagcttgtg attggtcac actttggtc gaaaatagc gttccggtg acggtcgct gtcgaagga ccggatcag gtgcaagca ccggaacac gaagaagtg gtctgtgaa actggacat tcgcggaac tcttcaaac acgagccaa ttatccaaa gcttccggt tacagcagc atccattcg cattgtcac agccggcag tcgagctcg ggcgattct ggacatatc agggctcta tcacacaga caaaaccgg aaacaaaat ggtaacccg ttcctcaac tcctcaaat tttattcgc gtaaacact ttcccgaac cgaatccga atccctaac acgataatc cacgaagac gttttaaac agcacttta gagacttta actccggtt aaatcaccc gaaaacgga gaaaccacg cgttactac actcccgat ggttaccac acacaattg agtaacgtt agcggttca agcaccgac gattacaaa actcccgat gaatcttta gacatgcga accgattta ttttcgcag aaattcacc gaactgcga aaatcctca tcgactagc ttaatcgaa ttgaacgcg cagccaaac gaatccgat ttgttgctg gacaacaat cgtttaagc gcggacaat gctattaaa atcaaacaa acggacggt gtattaaaa tcgaaaagc gactttgaa atccctcga gtcagtccg attcaaaag actcgtttc cgaggcggt tttttccgt tcggataat ttaccggcg tttttaaca ccgattctt aagcggaaa agtgcgcaa tcaaccccc aaaaatccc gacttcact tccctcaac tcgcttcgc acggggacg gccgggact gaccaattg ggcacccag ccaagaaat ccgagctca agcagttta gtcctgtcc gaaaccggt tcactagat cgagcaaag gcggcttac gagcggcgt aaaaagacg caaattcag gattccgat acgggggtg gtttccgga agggtggaa aatactcgt gttaatgcc aatcggcta atagcggag attttgaaa gatacgaat ctagagcaa gctgatgat tcggctgaa acttcaggt cttcaattg ttcattgcc aaagatggg actgctgcg ttagggaat cacgaagtc aagtcgcag atgtcaact ggggcgcaa gtttttaaa caagttgtt atggatgac aacaggtag
- >TC004825 RB
atggcgtcc ttcatgacg aaaaagaag aagtacaaa tttcaagtg agtctatgt ttggaagaa ctgctggaa gtgcctttc gtttctgcc gtccttttt gccaagata agactccta gatggggga aattttcag gaccatagc agcagagag gaggtgcgc gaccacaaa gtgaaatgg ggcgcaaac ttttccttc ccttgtaaa atgtcagcg aacgcatcc accggcgtc ctcgagcgt tgtatttta cggatatcc gtgcgaaag gagtgcaaa ggcggccgt tcatttacc aaactaggt ttcgtcgac ttaaatcta gcagaatac gcaggggcg ggtgtcacg ttcaagaaa gctttatta gaaggatac gacgccaga catcgtcaa gacaacagc atgctcaag ttttccatt gcaatgcat atgttatcg ggtgatatt ttattcaaa gtaccgtct ccttccctc aaacataaa tctatagct tccgaagat ccagcaacc gaatctcga acggatgag ttttcgagt gagtcgctg ggtggttcc attgcaagt ggaagttcc ggatttggg agtttgccc aaaaaacga ccagcgctt ttatcgtca gagcttgtg attggtcac actttggtc gaaaatagc gttccggtg acggtcgct gtcgaagga ccggatcag gtgcaagca ccggaacac gaagaagtg gtctgtgaa actggacat tcgcggaac tcttcaaac acgagccaa ttatccaaa gcttccggt tacagcagc atccattcg cattgtcac agccggcag tcgagctcg ggcgattct ggacatatc agggctcta tcacacaga caaaaccgg aaacaaaat ggtaacccg ttcctcaac tcctcaaat tttattcgc gtaaacact ttcccgaac cgaatccga atccctaac acgataatc cacgaagac gttttaaac agcacttta gagacttta actccggtt aaatcaccc gaaaacgga gaaaccacg cgttactac actcccgat ggttaccac acacaattg agtaacgtt agcggttca agcaccgac gattacaaa actcccgat gaatcttta gacatgcga accgattta ttttcgcag aaattcacc gaactgcga aaatcctca tcgactagc ttaatcgaa ttgaacgcg cagccaaac gaatccgat ttgttgctg gacaacaat cgtttaagc gcggacaat gctattaaa atcaaacaa acggacggt gtattaaaa tcgaaaagc gactttgaa atccctcga gtcagtccg attcaaaag actcgtttc cgaggcggt tttttccgt tcggataat ttaccggcg tttttaaca ccgattctt aagcggaaa agtgcgcaa tcaaccccc aaaaatccc gacttcact tccctcaac tcgcttcgc acggggacg gccgggact gaccaattg ggcacccag ccaaggggc ttgccacat cgcaaatta ctggagggt gcgtcagga ggtaagcta gctccgttg gccatcagt cctgatgaa acgcctacc actgtatcg tctgattgc tcacaaaac ctcacaatt cgacgtccc agcgtcaca acaaatccg agctcaagc agtttagtc ctgtccgaa accggttca ctagatcga gcaaaggcg gcttacgag cggcgtaaa aagacgcaa attcaggat tccgatacg ggggtggtt tccggaagg gtggaaaat actcgtgtt aatgccaat cggctaata gcggagatt ttgaaagat acgaatcta gagcaagct gatgattcg gctgaaact tcaggtctt caattgttc attgccaaa gatgggact gctgcgtta gggaatcac gaagtcaag tcgcagatg tcaactggg gcgcaagtt tttaaacaa gttgttatg gatgacaac aggtag
- >TC004825 RA
cgataatgg cgtttgtgt aacggtgta gtgtcgagc tttttattg aatattttg aattacaaa atagagaat tgcaacgag tgttagtta ccacaacta tgatggaaa ataagtgga ttccattat gaggcacga ttgaaagcg gaagttgtc tttgtccga atcgtttat aaagcgatc gtttgatat cgctgtttc ggaagcgat gtcttgagc cagttttct gcatgacaa acggacgag gcgccggaa tggcgtcct tcatgacga aaaagaaga agtacaaat ttcaagtga gtctatgtt tggaagaac tgctggaag tgcctttcg tttctgccg tcctttttg ccaagataa gactcctag atgggggaa attttcagg accatagca gcagagagg aggtgcgcg accacaaag tgaaatggg gcgcaaact tttccttcc cttgtaaaa tgtcagcga acgcatcca ccggcgtcc tcgagcgtt gtattttac ggatatccg tgcgaaagg agtgcaaag gcggccgtt catttacca aactaggtt tcgtcgact taaatctag cagaatacg caggggcgg gtgtcacgt tcaagaaag ctttattag aaggatacg acgccagac atcgtcaag acaacagca tgctcaagt tttccattg caatgcata tgttatcgg gtgatattt tattcaaag taccgtctc cttccctca aacataaat ctatagctt ccgaagatc cagcaaccg aatctcgaa cggatgagt tttcgagtg agtcgctgg gtggttcca ttgcaagtg gaagttccg gatttggga gtttgccca aaaaacgac cagcgcttt tatcgtcag agcttgtga ttggtcaca ctttggtcg aaaatagcg ttccggtga cggtcgctg tcgaaggac cggatcagg tgcaagcac cggaacacg aagaagtgg tctgtgaaa ctggacatt cgcggaact cttcaaaca cgagccaat tatccaaag cttccggtt acagcagca tccattcgc attgtcaca gccggcagt cgagctcgg gcgattctg gacatatca gggctctat cacacagac aaaaccgga aacaaaatg gtaacccgt tcctcaact cctcaaatt ttattcgcg taaacactt tcccgaacc gaatccgaa tccctaaca cgataatcc acgaagacg ttttaaaca gcactttag agactttaa ctccggtta aatcacccg aaaacggag aaaccacgc gttactaca ctcccgatg gttaccaca cacaattga gtaacgtta gcggttcaa gcaccgacg attacaaaa ctcccgatg aatctttag acatgcgaa ccgatttat tttcgcaga aattcaccg aactgcgaa aatcctcat cgactagct taatcgaat tgaacgcgc agccaaacg aatccgatt tgttgctgg acaacaatc gtttaagcg cggacaatg ctattaaaa tcaaacaaa cggacggtg tattaaaat cgaaaagcg actttgaaa tccctcgag tcagtccga ttcaaaaga ctcgtttcc gaggcggtt ttttccgtt cggataatt taccggcgt ttttaacac cgattctta agcggaaaa gtgcgcaat caaccccca aaaatcccg acttcactt ccctcaact cgcttcgca cggggacgg ccgggactg accaattgg gcacccagc caagaaatc cgagctcaa gcagtttag tcctgtccg aaaccggtt cactagatc gagcaaagg cggcttacg agcggcgta aaaagacgc aaattcagg attccgata cgggggtgg tttccggaa gggtggaaa atactcgtg ttaatgcca atcggctaa tagcggaga ttttgaaag atacgaatc tagagcaag ctgatgatt cggctgaaa cttcaggtc ttcaattgt tcattgcca aagatggga ctgctgcgt tagggaatc acgaagtca agtcgcaga tgtcaactg gggcgcaag tttttaaac aagttgtta tggatgaca acaggtagt cgatatgtg tggtgtgtg tcgcagttt aggagcttc tttaagttt gacagagaa atgaaagca gtgacatct cattttcat gtcaatagg acggattta ctagacagt gttgccaat ttttgtgat ataacagcg gtagtaaac gttattgga actcttatt taagttgaa atgacatta tttattgta aattattat tgtgttaaa tgtatatta tttattatt gatttttaa attattagt acactgtat aaagtcgtt ttaaaatgt acataaata caaacatga ataaaaact tagacctaa c
- >TC004825 RB
cgataatgg cgtttgtgt aacggtgta gtgtcgagc tttttattg aatattttg aattacaaa atagagaat tgcaacgag tgttagtta ccacaacta tgatggaaa ataagtgga ttccattat gaggcacga ttgaaagcg gaagttgtc tttgtccga atcgtttat aaagcgatc gtttgatat cgctgtttc ggaagcgat gtcttgagc cagttttct gcatgacaa acggacgag gcgccggaa tggcgtcct tcatgacga aaaagaaga agtacaaat ttcaagtga gtctatgtt tggaagaac tgctggaag tgcctttcg tttctgccg tcctttttg ccaagataa gactcctag atgggggaa attttcagg accatagca gcagagagg aggtgcgcg accacaaag tgaaatggg gcgcaaact tttccttcc cttgtaaaa tgtcagcga acgcatcca ccggcgtcc tcgagcgtt gtattttac ggatatccg tgcgaaagg agtgcaaag gcggccgtt catttacca aactaggtt tcgtcgact taaatctag cagaatacg caggggcgg gtgtcacgt tcaagaaag ctttattag aaggatacg acgccagac atcgtcaag acaacagca tgctcaagt tttccattg caatgcata tgttatcgg gtgatattt tattcaaag taccgtctc cttccctca aacataaat ctatagctt ccgaagatc cagcaaccg aatctcgaa cggatgagt tttcgagtg agtcgctgg gtggttcca ttgcaagtg gaagttccg gatttggga gtttgccca aaaaacgac cagcgcttt tatcgtcag agcttgtga ttggtcaca ctttggtcg aaaatagcg ttccggtga cggtcgctg tcgaaggac cggatcagg tgcaagcac cggaacacg aagaagtgg tctgtgaaa ctggacatt cgcggaact cttcaaaca cgagccaat tatccaaag cttccggtt acagcagca tccattcgc attgtcaca gccggcagt cgagctcgg gcgattctg gacatatca gggctctat cacacagac aaaaccgga aacaaaatg gtaacccgt tcctcaact cctcaaatt ttattcgcg taaacactt tcccgaacc gaatccgaa tccctaaca cgataatcc acgaagacg ttttaaaca gcactttag agactttaa ctccggtta aatcacccg aaaacggag aaaccacgc gttactaca ctcccgatg gttaccaca cacaattga gtaacgtta gcggttcaa gcaccgacg attacaaaa ctcccgatg aatctttag acatgcgaa ccgatttat tttcgcaga aattcaccg aactgcgaa aatcctcat cgactagct taatcgaat tgaacgcgc agccaaacg aatccgatt tgttgctgg acaacaatc gtttaagcg cggacaatg ctattaaaa tcaaacaaa cggacggtg tattaaaat cgaaaagcg actttgaaa tccctcgag tcagtccga ttcaaaaga ctcgtttcc gaggcggtt ttttccgtt cggataatt taccggcgt ttttaacac cgattctta agcggaaaa gtgcgcaat caaccccca aaaatcccg acttcactt ccctcaact cgcttcgca cggggacgg ccgggactg accaattgg gcacccagc caaggggct tgccacatc gcaaattac tggagggtg cgtcaggag gtaagctag ctccgttgg ccatcagtc ctgatgaaa cgcctacca ctgtatcgt ctgattgct cacaaaacc tcacaattc gacgtccca gcgtcacaa caaatccga gctcaagca gtttagtcc tgtccgaaa ccggttcac tagatcgag caaaggcgg cttacgagc ggcgtaaaa agacgcaaa ttcaggatt ccgatacgg gggtggttt ccggaaggg tggaaaata ctcgtgtta atgccaatc ggctaatag cggagattt tgaaagata cgaatctag agcaagctg atgattcgg ctgaaactt caggtcttc aattgttca ttgccaaag atgggactg ctgcgttag ggaatcacg aagtcaagt cgcagatgt caactgggg cgcaagttt ttaaacaag ttgttatgg atgacaaca ggtagtcga tatgtgtgg tgtgtgtcg cagtttagg agcttcttt aagtttgac agagaaatg aaagcagtg acatctcat tttcatgtc aataggacg gatttacta gacagtgtt gccaatttt tgtgatata acagcggta gtaaacgtt attggaact cttatttaa gttgaaatg acattattt attgtaaat tattattgt gttaaatgt atattattt attattgat ttttaaatt attagtaca ctgtataaa gtcgtttta aaatgtaca taaatacaa acatgaata aaaacttag acctaac
