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Gene information
General information
TC004825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 368006..381526
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004825
    iBeetle screen iB_03852
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004825 RA
      MASFMTKKK KYKFQVSLC LEELLEVPF VSAVLFAKI 
      RLLDGGNFQ DHSSREEVR DHKVKWGAN FSFPCKMSA 
      NASTGVLER CILRISVRK ECKGGRSFT KLGFVDLNL 
      AEYAGAGVT FKKALLEGY DARHRQDNS MLKFSIAMH 
      MLSGDILFK VPSPSLKHK SIASEDPAT ESRTDEFSS 
      ESLGGSIAS GSSGFGSLP KKRPALLSS ELVIGHTLV 
      ENSVPVTVA VEGPDQVQA PEHEEVVCE TGHSRNSSN 
      TSQLSKASG YSSIHSHCH SRQSSSGDS GHIRALSHR 
      QNRKQNGNP FLNSSNFIR VNTFPNRIR IPNTIIHED 
      VLNSTLETL TPVKSPENG ETTRYYTPD GYHTQLSNV 
      SGSSTDDYK TPDESLDMR TDLFSQKFT ELRKSSSTS 
      LIELNAQPN ESDLLLDNN RLSADNAIK IKQTDGVLK 
      SKSDFEIPR VSPIQKTRF RGGFFRSDN LPAFLTPIL 
      KRKSAQSTP KNPDFTSLN SLRTGTAGT DQLGTQPRN 
      PSSSSLVLS ETGSLDRAK AAYERRKKT QIQDSDTGV 
      VSGRVENTR VNANRLIAE ILKDTNLEQ ADDSAETSG 
      LQLFIAKDG TAALGNHEV KSQMSTGAQ VFKQVVMDD 
      NR
    • >TC004825 RB
      MASFMTKKK KYKFQVSLC LEELLEVPF VSAVLFAKI 
      RLLDGGNFQ DHSSREEVR DHKVKWGAN FSFPCKMSA 
      NASTGVLER CILRISVRK ECKGGRSFT KLGFVDLNL 
      AEYAGAGVT FKKALLEGY DARHRQDNS MLKFSIAMH 
      MLSGDILFK VPSPSLKHK SIASEDPAT ESRTDEFSS 
      ESLGGSIAS GSSGFGSLP KKRPALLSS ELVIGHTLV 
      ENSVPVTVA VEGPDQVQA PEHEEVVCE TGHSRNSSN 
      TSQLSKASG YSSIHSHCH SRQSSSGDS GHIRALSHR 
      QNRKQNGNP FLNSSNFIR VNTFPNRIR IPNTIIHED 
      VLNSTLETL TPVKSPENG ETTRYYTPD GYHTQLSNV 
      SGSSTDDYK TPDESLDMR TDLFSQKFT ELRKSSSTS 
      LIELNAQPN ESDLLLDNN RLSADNAIK IKQTDGVLK 
      SKSDFEIPR VSPIQKTRF RGGFFRSDN LPAFLTPIL 
      KRKSAQSTP KNPDFTSLN SLRTGTAGT DQLGTQPRG 
      LPHRKLLEG ASGGKLAPL AISPDETPT TVSSDCSQN 
      LTIRRPSVT TNPSSSSLV LSETGSLDR AKAAYERRK 
      KTQIQDSDT GVVSGRVEN TRVNANRLI AEILKDTNL 
      EQADDSAET SGLQLFIAK DGTAALGNH EVKSQMSTG 
      AQVFKQVVM DDNR
    CDS sequence
    • >TC004825 RA
      atggcgtcc ttcatgacg aaaaagaag aagtacaaa 
      tttcaagtg agtctatgt ttggaagaa ctgctggaa 
      gtgcctttc gtttctgcc gtccttttt gccaagata 
      agactccta gatggggga aattttcag gaccatagc 
      agcagagag gaggtgcgc gaccacaaa gtgaaatgg 
      ggcgcaaac ttttccttc ccttgtaaa atgtcagcg 
      aacgcatcc accggcgtc ctcgagcgt tgtatttta 
      cggatatcc gtgcgaaag gagtgcaaa ggcggccgt 
      tcatttacc aaactaggt ttcgtcgac ttaaatcta 
      gcagaatac gcaggggcg ggtgtcacg ttcaagaaa 
      gctttatta gaaggatac gacgccaga catcgtcaa 
      gacaacagc atgctcaag ttttccatt gcaatgcat 
      atgttatcg ggtgatatt ttattcaaa gtaccgtct 
      ccttccctc aaacataaa tctatagct tccgaagat 
      ccagcaacc gaatctcga acggatgag ttttcgagt 
      gagtcgctg ggtggttcc attgcaagt ggaagttcc 
      ggatttggg agtttgccc aaaaaacga ccagcgctt 
      ttatcgtca gagcttgtg attggtcac actttggtc 
      gaaaatagc gttccggtg acggtcgct gtcgaagga 
      ccggatcag gtgcaagca ccggaacac gaagaagtg 
      gtctgtgaa actggacat tcgcggaac tcttcaaac 
      acgagccaa ttatccaaa gcttccggt tacagcagc 
      atccattcg cattgtcac agccggcag tcgagctcg 
      ggcgattct ggacatatc agggctcta tcacacaga 
      caaaaccgg aaacaaaat ggtaacccg ttcctcaac 
      tcctcaaat tttattcgc gtaaacact ttcccgaac 
      cgaatccga atccctaac acgataatc cacgaagac 
      gttttaaac agcacttta gagacttta actccggtt 
      aaatcaccc gaaaacgga gaaaccacg cgttactac 
      actcccgat ggttaccac acacaattg agtaacgtt 
      agcggttca agcaccgac gattacaaa actcccgat 
      gaatcttta gacatgcga accgattta ttttcgcag 
      aaattcacc gaactgcga aaatcctca tcgactagc 
      ttaatcgaa ttgaacgcg cagccaaac gaatccgat 
      ttgttgctg gacaacaat cgtttaagc gcggacaat 
      gctattaaa atcaaacaa acggacggt gtattaaaa 
      tcgaaaagc gactttgaa atccctcga gtcagtccg 
      attcaaaag actcgtttc cgaggcggt tttttccgt 
      tcggataat ttaccggcg tttttaaca ccgattctt 
      aagcggaaa agtgcgcaa tcaaccccc aaaaatccc 
      gacttcact tccctcaac tcgcttcgc acggggacg 
      gccgggact gaccaattg ggcacccag ccaagaaat 
      ccgagctca agcagttta gtcctgtcc gaaaccggt 
      tcactagat cgagcaaag gcggcttac gagcggcgt 
      aaaaagacg caaattcag gattccgat acgggggtg 
      gtttccgga agggtggaa aatactcgt gttaatgcc 
      aatcggcta atagcggag attttgaaa gatacgaat 
      ctagagcaa gctgatgat tcggctgaa acttcaggt 
      cttcaattg ttcattgcc aaagatggg actgctgcg 
      ttagggaat cacgaagtc aagtcgcag atgtcaact 
      ggggcgcaa gtttttaaa caagttgtt atggatgac 
      aacaggtag
    • >TC004825 RB
      atggcgtcc ttcatgacg aaaaagaag aagtacaaa 
      tttcaagtg agtctatgt ttggaagaa ctgctggaa 
      gtgcctttc gtttctgcc gtccttttt gccaagata 
      agactccta gatggggga aattttcag gaccatagc 
      agcagagag gaggtgcgc gaccacaaa gtgaaatgg 
      ggcgcaaac ttttccttc ccttgtaaa atgtcagcg 
      aacgcatcc accggcgtc ctcgagcgt tgtatttta 
      cggatatcc gtgcgaaag gagtgcaaa ggcggccgt 
      tcatttacc aaactaggt ttcgtcgac ttaaatcta 
      gcagaatac gcaggggcg ggtgtcacg ttcaagaaa 
      gctttatta gaaggatac gacgccaga catcgtcaa 
      gacaacagc atgctcaag ttttccatt gcaatgcat 
      atgttatcg ggtgatatt ttattcaaa gtaccgtct 
      ccttccctc aaacataaa tctatagct tccgaagat 
      ccagcaacc gaatctcga acggatgag ttttcgagt 
      gagtcgctg ggtggttcc attgcaagt ggaagttcc 
      ggatttggg agtttgccc aaaaaacga ccagcgctt 
      ttatcgtca gagcttgtg attggtcac actttggtc 
      gaaaatagc gttccggtg acggtcgct gtcgaagga 
      ccggatcag gtgcaagca ccggaacac gaagaagtg 
      gtctgtgaa actggacat tcgcggaac tcttcaaac 
      acgagccaa ttatccaaa gcttccggt tacagcagc 
      atccattcg cattgtcac agccggcag tcgagctcg 
      ggcgattct ggacatatc agggctcta tcacacaga 
      caaaaccgg aaacaaaat ggtaacccg ttcctcaac 
      tcctcaaat tttattcgc gtaaacact ttcccgaac 
      cgaatccga atccctaac acgataatc cacgaagac 
      gttttaaac agcacttta gagacttta actccggtt 
      aaatcaccc gaaaacgga gaaaccacg cgttactac 
      actcccgat ggttaccac acacaattg agtaacgtt 
      agcggttca agcaccgac gattacaaa actcccgat 
      gaatcttta gacatgcga accgattta ttttcgcag 
      aaattcacc gaactgcga aaatcctca tcgactagc 
      ttaatcgaa ttgaacgcg cagccaaac gaatccgat 
      ttgttgctg gacaacaat cgtttaagc gcggacaat 
      gctattaaa atcaaacaa acggacggt gtattaaaa 
      tcgaaaagc gactttgaa atccctcga gtcagtccg 
      attcaaaag actcgtttc cgaggcggt tttttccgt 
      tcggataat ttaccggcg tttttaaca ccgattctt 
      aagcggaaa agtgcgcaa tcaaccccc aaaaatccc 
      gacttcact tccctcaac tcgcttcgc acggggacg 
      gccgggact gaccaattg ggcacccag ccaaggggc 
      ttgccacat cgcaaatta ctggagggt gcgtcagga 
      ggtaagcta gctccgttg gccatcagt cctgatgaa 
      acgcctacc actgtatcg tctgattgc tcacaaaac 
      ctcacaatt cgacgtccc agcgtcaca acaaatccg 
      agctcaagc agtttagtc ctgtccgaa accggttca 
      ctagatcga gcaaaggcg gcttacgag cggcgtaaa 
      aagacgcaa attcaggat tccgatacg ggggtggtt 
      tccggaagg gtggaaaat actcgtgtt aatgccaat 
      cggctaata gcggagatt ttgaaagat acgaatcta 
      gagcaagct gatgattcg gctgaaact tcaggtctt 
      caattgttc attgccaaa gatgggact gctgcgtta 
      gggaatcac gaagtcaag tcgcagatg tcaactggg 
      gcgcaagtt tttaaacaa gttgttatg gatgacaac 
      aggtag
    mRNA sequence
    • >TC004825 RA
      cgataatgg cgtttgtgt aacggtgta gtgtcgagc 
      tttttattg aatattttg aattacaaa atagagaat 
      tgcaacgag tgttagtta ccacaacta tgatggaaa 
      ataagtgga ttccattat gaggcacga ttgaaagcg 
      gaagttgtc tttgtccga atcgtttat aaagcgatc 
      gtttgatat cgctgtttc ggaagcgat gtcttgagc 
      cagttttct gcatgacaa acggacgag gcgccggaa 
      tggcgtcct tcatgacga aaaagaaga agtacaaat 
      ttcaagtga gtctatgtt tggaagaac tgctggaag 
      tgcctttcg tttctgccg tcctttttg ccaagataa 
      gactcctag atgggggaa attttcagg accatagca 
      gcagagagg aggtgcgcg accacaaag tgaaatggg 
      gcgcaaact tttccttcc cttgtaaaa tgtcagcga 
      acgcatcca ccggcgtcc tcgagcgtt gtattttac 
      ggatatccg tgcgaaagg agtgcaaag gcggccgtt 
      catttacca aactaggtt tcgtcgact taaatctag 
      cagaatacg caggggcgg gtgtcacgt tcaagaaag 
      ctttattag aaggatacg acgccagac atcgtcaag 
      acaacagca tgctcaagt tttccattg caatgcata 
      tgttatcgg gtgatattt tattcaaag taccgtctc 
      cttccctca aacataaat ctatagctt ccgaagatc 
      cagcaaccg aatctcgaa cggatgagt tttcgagtg 
      agtcgctgg gtggttcca ttgcaagtg gaagttccg 
      gatttggga gtttgccca aaaaacgac cagcgcttt 
      tatcgtcag agcttgtga ttggtcaca ctttggtcg 
      aaaatagcg ttccggtga cggtcgctg tcgaaggac 
      cggatcagg tgcaagcac cggaacacg aagaagtgg 
      tctgtgaaa ctggacatt cgcggaact cttcaaaca 
      cgagccaat tatccaaag cttccggtt acagcagca 
      tccattcgc attgtcaca gccggcagt cgagctcgg 
      gcgattctg gacatatca gggctctat cacacagac 
      aaaaccgga aacaaaatg gtaacccgt tcctcaact 
      cctcaaatt ttattcgcg taaacactt tcccgaacc 
      gaatccgaa tccctaaca cgataatcc acgaagacg 
      ttttaaaca gcactttag agactttaa ctccggtta 
      aatcacccg aaaacggag aaaccacgc gttactaca 
      ctcccgatg gttaccaca cacaattga gtaacgtta 
      gcggttcaa gcaccgacg attacaaaa ctcccgatg 
      aatctttag acatgcgaa ccgatttat tttcgcaga 
      aattcaccg aactgcgaa aatcctcat cgactagct 
      taatcgaat tgaacgcgc agccaaacg aatccgatt 
      tgttgctgg acaacaatc gtttaagcg cggacaatg 
      ctattaaaa tcaaacaaa cggacggtg tattaaaat 
      cgaaaagcg actttgaaa tccctcgag tcagtccga 
      ttcaaaaga ctcgtttcc gaggcggtt ttttccgtt 
      cggataatt taccggcgt ttttaacac cgattctta 
      agcggaaaa gtgcgcaat caaccccca aaaatcccg 
      acttcactt ccctcaact cgcttcgca cggggacgg 
      ccgggactg accaattgg gcacccagc caagaaatc 
      cgagctcaa gcagtttag tcctgtccg aaaccggtt 
      cactagatc gagcaaagg cggcttacg agcggcgta 
      aaaagacgc aaattcagg attccgata cgggggtgg 
      tttccggaa gggtggaaa atactcgtg ttaatgcca 
      atcggctaa tagcggaga ttttgaaag atacgaatc 
      tagagcaag ctgatgatt cggctgaaa cttcaggtc 
      ttcaattgt tcattgcca aagatggga ctgctgcgt 
      tagggaatc acgaagtca agtcgcaga tgtcaactg 
      gggcgcaag tttttaaac aagttgtta tggatgaca 
      acaggtagt cgatatgtg tggtgtgtg tcgcagttt 
      aggagcttc tttaagttt gacagagaa atgaaagca 
      gtgacatct cattttcat gtcaatagg acggattta 
      ctagacagt gttgccaat ttttgtgat ataacagcg 
      gtagtaaac gttattgga actcttatt taagttgaa 
      atgacatta tttattgta aattattat tgtgttaaa 
      tgtatatta tttattatt gatttttaa attattagt 
      acactgtat aaagtcgtt ttaaaatgt acataaata 
      caaacatga ataaaaact tagacctaa c
    • >TC004825 RB
      cgataatgg cgtttgtgt aacggtgta gtgtcgagc 
      tttttattg aatattttg aattacaaa atagagaat 
      tgcaacgag tgttagtta ccacaacta tgatggaaa 
      ataagtgga ttccattat gaggcacga ttgaaagcg 
      gaagttgtc tttgtccga atcgtttat aaagcgatc 
      gtttgatat cgctgtttc ggaagcgat gtcttgagc 
      cagttttct gcatgacaa acggacgag gcgccggaa 
      tggcgtcct tcatgacga aaaagaaga agtacaaat 
      ttcaagtga gtctatgtt tggaagaac tgctggaag 
      tgcctttcg tttctgccg tcctttttg ccaagataa 
      gactcctag atgggggaa attttcagg accatagca 
      gcagagagg aggtgcgcg accacaaag tgaaatggg 
      gcgcaaact tttccttcc cttgtaaaa tgtcagcga 
      acgcatcca ccggcgtcc tcgagcgtt gtattttac 
      ggatatccg tgcgaaagg agtgcaaag gcggccgtt 
      catttacca aactaggtt tcgtcgact taaatctag 
      cagaatacg caggggcgg gtgtcacgt tcaagaaag 
      ctttattag aaggatacg acgccagac atcgtcaag 
      acaacagca tgctcaagt tttccattg caatgcata 
      tgttatcgg gtgatattt tattcaaag taccgtctc 
      cttccctca aacataaat ctatagctt ccgaagatc 
      cagcaaccg aatctcgaa cggatgagt tttcgagtg 
      agtcgctgg gtggttcca ttgcaagtg gaagttccg 
      gatttggga gtttgccca aaaaacgac cagcgcttt 
      tatcgtcag agcttgtga ttggtcaca ctttggtcg 
      aaaatagcg ttccggtga cggtcgctg tcgaaggac 
      cggatcagg tgcaagcac cggaacacg aagaagtgg 
      tctgtgaaa ctggacatt cgcggaact cttcaaaca 
      cgagccaat tatccaaag cttccggtt acagcagca 
      tccattcgc attgtcaca gccggcagt cgagctcgg 
      gcgattctg gacatatca gggctctat cacacagac 
      aaaaccgga aacaaaatg gtaacccgt tcctcaact 
      cctcaaatt ttattcgcg taaacactt tcccgaacc 
      gaatccgaa tccctaaca cgataatcc acgaagacg 
      ttttaaaca gcactttag agactttaa ctccggtta 
      aatcacccg aaaacggag aaaccacgc gttactaca 
      ctcccgatg gttaccaca cacaattga gtaacgtta 
      gcggttcaa gcaccgacg attacaaaa ctcccgatg 
      aatctttag acatgcgaa ccgatttat tttcgcaga 
      aattcaccg aactgcgaa aatcctcat cgactagct 
      taatcgaat tgaacgcgc agccaaacg aatccgatt 
      tgttgctgg acaacaatc gtttaagcg cggacaatg 
      ctattaaaa tcaaacaaa cggacggtg tattaaaat 
      cgaaaagcg actttgaaa tccctcgag tcagtccga 
      ttcaaaaga ctcgtttcc gaggcggtt ttttccgtt 
      cggataatt taccggcgt ttttaacac cgattctta 
      agcggaaaa gtgcgcaat caaccccca aaaatcccg 
      acttcactt ccctcaact cgcttcgca cggggacgg 
      ccgggactg accaattgg gcacccagc caaggggct 
      tgccacatc gcaaattac tggagggtg cgtcaggag 
      gtaagctag ctccgttgg ccatcagtc ctgatgaaa 
      cgcctacca ctgtatcgt ctgattgct cacaaaacc 
      tcacaattc gacgtccca gcgtcacaa caaatccga 
      gctcaagca gtttagtcc tgtccgaaa ccggttcac 
      tagatcgag caaaggcgg cttacgagc ggcgtaaaa 
      agacgcaaa ttcaggatt ccgatacgg gggtggttt 
      ccggaaggg tggaaaata ctcgtgtta atgccaatc 
      ggctaatag cggagattt tgaaagata cgaatctag 
      agcaagctg atgattcgg ctgaaactt caggtcttc 
      aattgttca ttgccaaag atgggactg ctgcgttag 
      ggaatcacg aagtcaagt cgcagatgt caactgggg 
      cgcaagttt ttaaacaag ttgttatgg atgacaaca 
      ggtagtcga tatgtgtgg tgtgtgtcg cagtttagg 
      agcttcttt aagtttgac agagaaatg aaagcagtg 
      acatctcat tttcatgtc aataggacg gatttacta 
      gacagtgtt gccaatttt tgtgatata acagcggta 
      gtaaacgtt attggaact cttatttaa gttgaaatg 
      acattattt attgtaaat tattattgt gttaaatgt 
      atattattt attattgat ttttaaatt attagtaca 
      ctgtataaa gtcgtttta aaatgtaca taaatacaa 
      acatgaata aaaacttag acctaac