General information | |||
TC031968
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 2256234..2284550 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031968 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03851 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
maxillary palp structure not present - Number of animals showing the phenotype: 1
antenna orientation irregular - Number of animals showing the phenotype: 1
head orientation irregular - Number of animals showing the phenotype: 1
head size shortened - Number of animals showing the phenotype: 1
elytra ventralorientation not closed - Number of animals showing the phenotype: 1
elytra size shortened - Number of animals showing the phenotype: 1
legs orientation irregular - Number of animals showing the phenotype: 1
wings shortened - Number of animals showing the phenotype: 1
- >TC031968 RA
MPATIPDSP GSSPGIKDE CDDFMCIDD SPLDLRINC GRISPGARD SGTESDDTE EKIIQEGAD CKPYKKSLI KRFDKEQNN YLHQLLSRQ RTDLQHPSL CDYGRRKSV YPTTTTGSL PPSPADSGV SDVDSSSSG HTSTDELKA RLQPSIHSP VGNLFSRHP LSWTSPAHH GQRAPSHMP QQFYQPFTS IPDSYNSYL LQQHHHVQQ NQHMHHHNS TASPFTTPS PPSPPMRHA IPTSVIQAA TSSVYDDAY MLGFSPRKL KKVKKQKIG EPGSVKRKS REGSTTYLW EFLLKLLQD REYCPRYIK WTNREKGVF KLVDSKAVS RLWGMHKNK PDMNYETMG RALRYYYQR GILAKVDGQ RLVYQFVEV PKDIIEIDC TGA
- >TC031968 RA
atgccagcc accattccg gactcgccc ggatcctcc cccggaatc aaggatgaa tgcgacgat ttcatgtgc attgacgac tcacccctg gacttgaga atcaactgc ggccggatt agtccggga gcgcgcgat tccggaact gaaagcgac gacacggaa gaaaagatc atacaggaa ggcgccgac tgcaaaccc tacaaaaag agcctgatc aagagattt gacaaggag caaaacaac tacttacat caactactt tcaagacag cgtacagac ctccaacat ccctcattg tgtgattat ggcagaaga aagtcagtg tatcccacg acaaccacc ggttcgttg cctccgagt ccagcagat tcgggcgtt tcagacgtg gacagttcg tccagcggt cacacatca accgacgaa ttaaaagcg agattacag ccctcgata cattctccc gtaggaaat ttattttca agacaccca ttgtcatgg acgtcaccc gcccatcat ggtcagcga gctcccagt catatgccc cagcagttc taccaaccc ttcacctcc ataccggat tcgtacaac agctacctt ctccagcag caccaccac gtccagcag aaccagcac atgcaccac cacaacagc accgcctcc cctttcacg acgccctct cccccgtct ccgcccatg aggcacgcc atccccacc tcggtgatc caggcggcc acttcgagc gtgtacgat gacgcgtac atgctcggt ttttcccct cgaaaactg aagaaagtc aagaagcaa aaaatcggc gaacccggt tccgtcaaa aggaaaagt cgggagggt tcgacgacg tatctgtgg gaattcttg ctcaaactc ctgcaggac agggagtac tgcccgcgg tatatcaag tggacgaac agggagaag ggcgtcttc aagctggtg gactccaag gcggtgtcc aggctctgg ggcatgcac aagaacaag cccgatatg aattacgag acgatgggg cgggctctg aggtactac taccagagg ggcattttg gcgaaggtg gacggtcaa aggctggtt tatcagttc gttgaagtt ccgaaggat ataatcgaa atcgattgc acgggcgcg tga
- >TC031968 RA
attcacggc gtgtccaaa tgaaagtaa agaggcagg cactttgac gaaagctcc cgacgactc cacgaccgc cgtatctga gtcagtagg acattcgaa ccctactag tctgcactg aacggtgag gcaagcaag tagcgttaa gtacccctc tgtgcaact acacacgcg tgtggcaaa gtgtttaca ttgtgacag tgcgaggac ttcgattat gcgatcata aactcactt ttgatgatt ctgatcaag ccgttcagc aaaatgcca gccaccatt ccggactcg cccggatcc tcccccgga atcaaggat gaatgcgac gatttcatg tgcattgac gactcaccc ctggacttg agaatcaac tgcggccgg attagtccg ggagcgcgc gattccgga actgaaagc gacgacacg gaagaaaag atcatacag gaaggcgcc gactgcaaa ccctacaaa aagagcctg atcaagaga tttgacaag gagcaaaac aactactta catcaacta ctttcaaga cagcgtaca gacctccaa catccctca ttgtgtgat tatggcaga agaaagtca gtgtatccc acgacaacc accggttcg ttgcctccg agtccagca gattcgggc gtttcagac gtggacagt tcgtccagc ggtcacaca tcaaccgac gaattaaaa gcgagatta cagccctcg atacattct cccgtagga aatttattt tcaagacac ccattgtca tggacgtca cccgcccat catggtcag cgagctccc agtcatatg ccccagcag ttctaccaa cccttcacc tccataccg gattcgtac aacagctac cttctccag cagcaccac cacgtccag cagaaccag cacatgcac caccacaac agcaccgcc tcccctttc acgacgccc tctcccccg tctccgccc atgaggcac gccatcccc acctcggtg atccaggcg gccacttcg agcgtgtac gatgacgcg tacatgctc ggtttttcc cctcgaaaa ctgaagaaa gtcaagaag caaaaaatc ggcgaaccc ggttccgtc aaaaggaaa agtcgggag ggttcgacg acgtatctg tgggaattc ttgctcaaa ctcctgcag gacagggag tactgcccg cggtatatc aagtggacg aacagggag aagggcgtc ttcaagctg gtggactcc aaggcggtg tccaggctc tggggcatg cacaagaac aagcccgat atgaattac gagacgatg gggcgggct ctgaggtac tactaccag aggggcatt ttggcgaag gtggacggt caaaggctg gtttatcag ttcgttgaa gttccgaag gatataatc gaaatcgat tgcacgggc gcgtgattg ccttttaat tagacttct tccagtggt gtggaccac tgtctcgag agtacaaca tacttaaaa cacgaaatg aatttattt attagaaat tatacgtag gaactctgg tgtaaataa agacaatat tggaagagt tggttgaat tcatacaac gaattttcg cactttgca catttcgct ccgagatct caatgtaaa gtgtaacac gaatcactg tcatatctt cgtcatgga cctgatacg tcaatcaag aataagtgt cttgtcacc ttaactgga ctgctagga aggttgtga tgtgaatta tgttatcca ggttgtggt tactttaat aattaaacc agcaagaga ttgtaattt ttgcactat ttcataccc gttgcttcc cgagcaata ataaaattc cgacaaaaa tttggtttt tggcaaaca caaggtgct tcgcgtgtt aaattgtac agtgataat ttgttattt tgttgattg aaaagattt aactcgatt gcaatgtaa ataaaagtt tataaaagc cgtatggat gttgttgct tgtatgaaa tagtgatag taattgtta tactagatt tttataaag taagagata tttagatat tgtacatta cttaacaac ggtacgaag atttaatat gaaatgtga tagagagga taaagtatt ttggagacg ttttataaa ttttgttac ctagtttag cggtgctct tacaaggcg atgagactg tgcggtgcc tgaagattg acaaaacgc gttgaattg tcaatattt tagctctct tgagtgttc ggcagaaga ataaactac taaaagctt tgatctcag atgtacacg caggaaaac agtttgatt gaaaaacgc agaaaattg taaataaca ctgtttttg tacttatga ttgggttcc gtttctaat ccgtgtttc ctttacgtt tattttcta agtgtacat tctgtatag tatttattt aataacgtc aagaactca caatactaa gttgtaaat tttcgttgt aacgttctg tgattgtat atttagttc cctttgtaa aattttcga aattttcat tgtagtgaa tgttattaa cgttgtacc taaggtcaa atgtgtctt gaaatttta cccaactca gtgcgcaaa tcaggacga aattacaat tatattatt attaaaaac taacccagg tctcaatct cagtgaagt aactataca tatgtgtag aattattaa ctaaatgta cgcacaaca aacaaaata gtaacaaca ttcgaattg tacgtccaa aacatgtga taattgtcg aaaatgcat gcttgatca ccttagcat agatatatt tggtataca tgttcagtg gtcttttaa aacattttg tttgttgct gttggtccg ttcggtttc accttcatg tatacacat attgtttgt actctataa aaaagattt gaactaaac taacatttg ttgtaaaac agttattaa tgttaaaaa gagtaaata taaaaattg caactaaat aaaaaaaga ttggtgata ttgtcaaat gtaggataa taatgttga cacgcaagt tgtatatat tatattgta taattttta ttatattac aaaatacct acctaagta tctactgaa ttttaatga tttcaaata caatattgt atagtttta tttagcata agacaaggt acttttatt atatagttt attgtaata ttttgtctt tacaaaaat atgacatga ataaaatta tttgtgtta t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
