Status
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Gene information
General information
TC004801 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7413466..7421896
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004801
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03850

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004801 RA
      MAFAGLKKQ INKCNQYVT EKMGGAEGT KLDLDFMDM 
      ERKTDVTYE LVEELQIKT KEFLQPNPT ARAKMAAVK 
      GISKLSGQA KSNTYPQPE GVLGDCMLT YGRKLGDDS 
      MFGNALVEM GESLKQMAD VKYSLDDNI KQNFLEPLH 
      HLQTKDLKE VMHHRKKLQ GRRLDFDCK RRRQAKDDE 
      IKTAEDKFA ESLHLAQMG MFNLLENDV EQISQLATF 
      AEGLLEYHV QCTEILKHL NEILLEKKE EAANRPKVE 
      FVPKTLADL HIEGVSDGV NGIHHNNIN TNHHNFTNS 
      GHQFKRPAP KTIPIKQSD PFDPWSFPP ASPLPSPMK 
      SPARTPMNR QPCCTALYD FDPENPGEL GFKENDVIT 
      LLNKIDENW FEGSLHGRT GYFPVNYVK VDVPLP
    CDS sequence
    • >TC004801 RA
      atggcgttt gctggatta aagaaacag ataaataag 
      tgtaaccag tatgttacg gagaagatg ggaggtgcc 
      gaaggcact aaactggat ttggacttc atggacatg 
      gagaggaaa acggacgtc acgtatgaa ttagtcgaa 
      gaattacaa ataaaaacg aaagaattt ctgcaaccg 
      aatccgaca gcgcgcgcg aagatggcc gctgttaag 
      gggatcagc aaactaagc ggccaggcg aaatcaaac 
      acctacccc cagccggag ggtgtctta ggagactgc 
      atgctgacc tacggacgc aaactgggg gatgacagc 
      atgttcggg aacgctcta gtcgaaatg ggggaatca 
      cttaaacaa atggccgat gttaaatac tcgctcgac 
      gataatatt aagcaaaac ttccttgaa ccactccac 
      catttacaa actaaggac cttaaggaa gttatgcac 
      cacaggaaa aaactgcaa gggcgccgg ttggacttc 
      gactgtaag cgaagacgg caagctaaa gatgacgag 
      attaagact gcggaagat aagtttgca gagtctctg 
      catcttgct caaatggga atgttcaat ttattagaa 
      aatgacgta gaacaaatt tcacaatta gccacattc 
      gctgaagga ctgctggag taccacgtg cagtgcacg 
      gagatcctc aagcacttg aacgaaatc ctactggaa 
      aagaaagaa gaggccgca aatcgtccg aaagtcgaa 
      ttcgtgccg aaaaccctc gcggactta cacatagag 
      ggtgtttca gacggagtt aatggtatt catcacaac 
      aatatcaac acaaaccat cacaatttt acaaatagt 
      ggccaccag tttaaacgc cccgccccc aaaaccatt 
      cccatcaaa caatccgac ccgttcgac ccctggagc 
      tttccccca gcgtctcca ctgccgtcc ccaatgaaa 
      tcaccagca agaacgcca atgaaccga cagccttgt 
      tgtacagcg ctgtacgat ttcgatccc gaaaatccc 
      ggcgaattg ggctttaag gaaaacgat gtgatcact 
      ctgttaaat aaaatcgac gagaactgg ttcgagggg 
      agtctccac gggcgcacg ggttacttc ccagtaaat 
      tatgtgaaa gttgatgtg ccattacca tga
    mRNA sequence
    • >TC004801 RA
      ttcagtaaa attgcaatc gttagtcag tttgcaggt 
      gttttttgg tcgttgcca aatatccgt ttatttacc 
      aacagcgaa aacgtgaaa atcggcacg aaaaaatta 
      attggggta acgactaag tgaaaatgg cgtttgctg 
      gattaaaga aacagataa ataagtgta accagtatg 
      ttacggaga agatgggag gtgccgaag gcactaaac 
      tggatttgg acttcatgg acatggaga ggaaaacgg 
      acgtcacgt atgaattag tcgaagaat tacaaataa 
      aaacgaaag aatttctgc aaccgaatc cgacagcgc 
      gcgcgaaga tggccgctg ttaagggga tcagcaaac 
      taagcggcc aggcgaaat caaacacct acccccagc 
      cggagggtg tcttaggag actgcatgc tgacctacg 
      gacgcaaac tgggggatg acagcatgt tcgggaacg 
      ctctagtcg aaatggggg aatcactta aacaaatgg 
      ccgatgtta aatactcgc tcgacgata atattaagc 
      aaaacttcc ttgaaccac tccaccatt tacaaacta 
      aggacctta aggaagtta tgcaccaca ggaaaaaac 
      tgcaagggc gccggttgg acttcgact gtaagcgaa 
      gacggcaag ctaaagatg acgagatta agactgcgg 
      aagataagt ttgcagagt ctctgcatc ttgctcaaa 
      tgggaatgt tcaatttat tagaaaatg acgtagaac 
      aaatttcac aattagcca cattcgctg aaggactgc 
      tggagtacc acgtgcagt gcacggaga tcctcaagc 
      acttgaacg aaatcctac tggaaaaga aagaagagg 
      ccgcaaatc gtccgaaag tcgaattcg tgccgaaaa 
      ccctcgcgg acttacaca tagagggtg tttcagacg 
      gagttaatg gtattcatc acaacaata tcaacacaa 
      accatcaca attttacaa atagtggcc accagttta 
      aacgccccg cccccaaaa ccattccca tcaaacaat 
      ccgacccgt tcgacccct ggagctttc ccccagcgt 
      ctccactgc cgtccccaa tgaaatcac cagcaagaa 
      cgccaatga accgacagc cttgttgta cagcgctgt 
      acgatttcg atcccgaaa atcccggcg aattgggct 
      ttaaggaaa acgatgtga tcactctgt taaataaaa 
      tcgacgaga actggttcg aggggagtc tccacgggc 
      gcacgggtt acttcccag taaattatg tgaaagttg 
      atgtgccat taccatgag ttacgggtt aattaaatt 
      atacttcaa ttgaacaac aacccctgg tatctttgt 
      ttagtaata aatattgca tgccattac tgcgttact 
      tgcctttgt tttgtataa attgaagta tttatttgt 
      tgacgtgta ttcaactgc actctggta aaagtgaga 
      tgccatatt aagtagtca aacgtgcgg catttacct 
      tagagcgaa gctgacaac ttattaaat cttccttta 
      ctcaaatga tttatttcc attgtatat aacgtactg 
      taaattttt atttatgat attttaacg atttaggta 
      gcgtgtgat tggagcgtg cagttgaag tcgtttaat 
      tgtaattag ttcacgaga agttgcgta caaggttaa 
      gtttagttg tacatattt agtgctttt agcttagtt 
      attttaata ataatctag ttagtttat ataattgtt 
      tatatcgtg aaagcttat aatgttatg cagttacaa 
      gaggctaaa aattttata aaataagag atgtaatta 
      tggtacagt tgagatttt tgagagaaa actatatgc 
      tttgagttg aaacctaaa atgcgttga tttatcgcc 
      tctacctat cacaacttg ccaaatgtt aattgcatg 
      aaattgaaa ctcaatgtc ttaaaataa gaacatttt 
      tgatgtaga ttgatagat ttctgctga aactttatt 
      aattataat accaacaag gatttatat gcaaccacc 
      gagtatttg ggtgccgct ttttagaaa atgcactaa 
      ttatcgttt tgttttccg cagagtact gataaagtt 
      ttggccgaa tcactacaa ttgtttaac gttgtcatt 
      tgacatgct atagatgta ttccttata tttgtgtga 
      aaattaata ccagatatt gttcggctg tgtgttgtg 
      ctctgtctt cgctgtcac ttttctatt aattatcga 
      aaacgcctc tactcaaac ttggaacgt tgtccgctt 
      tggaatact cagatggtc aatatggag cataacgga 
      tagttttaa aagtactta cttatctgg ttcatgaca 
      atacattag atataaaag ttaatataa gtataacca 
      attaagaat attgttatt agatcaaac cgttatttt 
      ttacccttt caatcttgt tagtctaat tgtcctcag 
      tcatcactc caattgatc tcttcttta cttacataa 
      cacaggtaa acggtttta ctaaatttt acgtcatta 
      ttcgctttg ttctttaaa acaaaacga attgttatc 
      tattttcta aaaataaat gtcatattt ttgaagttt 
      ttttgactg cactgttac tcaaacttg aattcatta 
      ttggccata tatttttta aagtatagc atgttacca 
      aataatgag atattgtta atacacatg tatacaatt 
      taaatgtta ccgtatttg tgcattaat aaaaaaagg 
      tgtcatat
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