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Gene information
General information
TC004796 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7320500..7339104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004796
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03849

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    gnathal appendages randomremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004796 RA
      MKNNDETIL EILQGHKTS KWPAAKLVK IFIASTKAD 
FVEERRLLL EYVGPELQT VFDEQKIEV ELVDMFFGA 
SEKDTFDAK LFEDHLNEI RMCHKLSRG CFFLSLVGN 
KYEPLIVPP RLKSENFEA LVDKVGELG LNCDILENW 
YFKEGNEYI LKDCRFSTL EEWRAISEE LITIIHECL 
QNMPATTKK KFSSYLKSV VEQQFNFAL GLAPGTAHH 
VLNIFREWD VPEETEKFH QENSSHVNK TIENYCREQ 
IKLFHSNIK LLIPEENNL NFKVPWKPG GINTIHAEH 
EAYLNEFKS RVLNKLKDL IRQSLDDEP ELKSKKKIV 
EENFQENIT HLTLCYDDI KVDFSNSEI LERVKQIVQ 
NTTNVRHNP ILIYGNPGS GKTSLIQTI YRNFEKWFT 
CRTLRIIRF TSTTPRSSY ILELLRVIC QQICIALKL 
PEGFLPKDA SFDPLYINN WFQSLLRNF EDMNQILVI 
FIDDLHLLN PLDADAVNA LSWIPITLP KRVHMICTT 
STELDKLRM TPVQKERFK NPDSYLELP AVDPFDDYV 
NKKFDSLEE FFTKNAVAK ISSLISCSE YGLTETELL 
EILMPTSNS EAVIHVEDG NFNFSSLCS IRRKLEPLL 
REKIMSGKL LIEWSHVTI KEIVRKRYL HNQETLRST 
HSELANLFF YEFCSDESC DEEDEEGEP APLLSEIRE 
TPFQSTVYK DVTYSQRHI EEAWIHLLL AGDTIKLKS 
LCMCNYDFL LAALQTFSV SYLRCLLEH VRCYLLDRE 
IELVYYSIR KSTDVLTRD TYQLGTQLI SWLRPVIER 
GGGLMSSMV TSAMAWCDG FTLPLVVPL TDWLQPPLP 
QQSRVINTP NVRLIEPTP SGQHVVCVV DSEPQLWHI 
MSNQLVHTF KGHTGKIIC MAVTKHSQF LFTGSDDLS 
IIVWDLKSF GIKLRIHEH IAPVLCITG ALNNSVIIS 
GGEDSSIII TSLADGHVV MKIDHHRGP VTSVKVTST 
GDILVSGSH DGRVCLWSL ETYSLLNTI TLQSPIQMI 
EVSADSVFL LACCRDNNL YLRTLATGT ELHSLVEHK 
TKVRSICLT QDSCRAVIG GADGKVYIY DIHSAKLIK 
CLTGQNGEV SAVRVTEKD DFLLTAGGN RVIFYPFRS 
DDHIKSFSK MKKKSQHHL QAHGGFLTC LDISRDGQL 
SVTGALDHI VNVWQLNSQ ELVLTLKGH SGPITAVSF 
AANGLFVAS GSEDKTVKV WGLTLGTLV STFTGHQAA 
VSTVFVMMD STRIISSDR NDTLCIWLA DNGNLLQTY 
PGPSKCVRV TNNMKYAIA TNGDVSLKI WSLVKDDEK 
YNVNHSEKI TCFVLTIDS QHIITGSRD MSLKVWQVV 
GGKLSQVLI GHTDSVTCV AVSVSDKSQ VISGACDNN 
LIVWDINTG ADLYTLSAH LSYVTCVKL SGDGTIAIS 
GSDDKSIII WDTKRGLQL TSLQLHYPI ISIEPTSDF 
SRISVLLKD TNFMPIICL HNTPAKYVK LPTYCAPDK 
DIIENPKPA PKRQMKRLL KKEVSLDTY TWQKKYAHL 
TSNLVIPAV DERFKRRFS VSASMEEIS KIPQIKDSQ 
GGLGAKQAS LAQSQHFDQ LEALWNKRS PPRRRLHQS 
LSKQSSLAE SHLDSSDED GHLEDGKYA T
    CDS sequence
    • >TC004796 RA
      atgaagaat aacgatgag acaattctc gaaattctt 
caaggacac aaaacttct aaatggccg gctgcgaaa 
ctcgttaaa attttcatt gccagcact aaagcagat 
tttgttgaa gagcgacgt cttttgctt gaatatgtt 
ggtccagaa cttcaaacg gtattcgat gaacaaaag 
attgaggtc gaactagtc gatatgttt ttcggagcg 
tctgaaaag gataccttc gacgcgaaa ttattcgag 
gatcattta aacgaaata agaatgtgc cataagtta 
tcacgggga tgtttcttt ttgagttta gttgggaat 
aaatacgaa cctcttata gttcctcct cgtttaaaa 
tcggaaaat tttgaagcc ttagtcgac aaagtagga 
gaattgggg ctcaattgt gatatatta gaaaactgg 
tacttcaag gaaggaaac gaatatata ttaaaagac 
tgcagattc tcaacttta gaagaatgg agagctata 
tcagaagaa ttaatcact ataattcac gaatgtttg 
caaaacatg cctgcaaca acaaagaaa aaatttagc 
tcgtattta aaatcagtt gtcgaacaa cagtttaat 
tttgcttta gggttggca cccggcact gctcatcac 
gtgttaaac atttttaga gaatgggac gtacctgaa 
gagactgaa aaatttcat caggaaaat tctagccat 
gttaataaa actatagaa aactattgt cgtgaacaa 
ataaagttg tttcattcg aatataaaa ttacttatt 
ccagaagaa aataatctt aatttcaaa gtgccttgg 
aaacctggg ggaataaat acgatacat gcagaacac 
gaagcatat ctaaacgaa tttaaaagt cgtgtttta 
aataaatta aaagattta attagacaa agtttggac 
gatgaaccg gaattgaaa tctaaaaag aagatcgtc 
gaggagaac tttcaagaa aatataact cacttaact 
ctttgttat gacgatatc aaagtagat ttttccaat 
tcggaaatc ttggagcga gttaaacag atagtacaa 
aacactaca aatgttaga cataatcca attttgata 
tacggcaac cctggttcg ggaaaaacc tcgcttata 
caaacaata tacagaaac ttcgaaaag tggtttaca 
tgcagaact cttagaata ataaggttt acttcaacg 
acccctcga tcctcatat attttagag ctgttaaga 
gtcatatgc caacaaatt tgcatagca ttaaagctt 
ccagaaggt ttcttgcca aaagacgct tccttcgat 
cctttgtat attaacaac tggtttcaa agtttactc 
agaaatttc gaggatatg aatcaaatc cttgttata 
ttcatcgac gatttacat ttactcaat ccgttagac 
gccgacgcc gttaatgcc ctcagctgg ataccaata 
actctaccc aaaagggtc cacatgata tgcacaact 
tccacagaa ctggacaaa ctcagaatg acgccagtg 
caaaaagaa aggttcaaa aacccagac tcctacctc 
gaattaccc gcagttgat ccattcgac gactacgtg 
aataagaaa tttgacagt ttggaggag ttttttacg 
aaaaacgcg gtggcaaag atatcctcg ttgataagt 
tgttcggag tacggtctg actgagact gaacttttg 
gaaatttta atgccgacg agtaatagc gaggcagta 
attcatgtc gaagacggt aattttaat ttttcttcc 
ctgtgctcc atccggcga aagttggaa cctctcctt 
agggagaaa ataatgagc gggaaatta ctgatcgaa 
tggagtcac gtgacgatt aaagagatt gtgcggaag 
cgttatctc cacaatcag gaaacttta aggagcaca 
cattcggaa ttagctaac ttatttttc tacgagttt 
tgcagcgat gaaagctgc gacgaagag gacgaagaa 
ggggagcca gcaccgctt ttatccgaa attagggaa 
actccattt cagtcgacg gtttataaa gacgttacg 
tacagtcaa agacatatc gaggaagct tggatccat 
cttttgctc gctggtgat acaattaaa ctgaaaagc 
ttgtgtatg tgcaattac gacttcctt cttgccgct 
ttgcaaacg ttttcggtt agctacttg cgatgcttg 
ctggagcat gtccgatgt tatcttctt gatagagag 
atcgaattg gtttactac tctattagg aagtccacg 
gatgtatta actcgggat acttatcaa ttaggcact 
cagcttatc tcttggttg aggccggtc atagaacgt 
ggtggtggt ttgatgagc tctatggtc acttctgcc 
atggcttgg tgcgatggt ttcacttta cctcttgtg 
gttccatta acagactgg cttcaacca cctcttcca 
caacaatcc cgcgtcatt aacacaccg aatgtgcga 
cttatcgaa cccactcct tccggacaa catgttgtt 
tgtgttgtc gattccgag ccgcagctt tggcacata 
atgagcaac caattagtg cacaccttt aaaggtcat 
acggggaaa ataatttgt atggctgtg accaaacat 
tcccagttc ttgtttacc ggttcggat gatttaagt 
ataatcgtt tgggacttg aaatcattc ggtattaaa 
ttacgaatt catgaacac atcgcaccg gttttgtgc 
attaccggc gctctcaat aactcggtc attatcagt 
ggaggagag gacagtagc atcattata acttctctg 
gcagatgga cacgtcgtt atgaaaata gaccaccat 
cgaggcccc gtaacttcc gtcaaagtc acttcgacg 
ggtgacatc ttagtttcg ggaagtcat gatggaaga 
gtctgtttg tggtctttg gaaacttac agtttgctt 
aatacgatt acgctacaa tcgccgata caaatgatc 
gaagtttcg gccgattcg gtctttcta ttagcttgt 
tgtagggac aacaatctc tatttacgc acattggcg 
acgggaacc gaactacac agtttagtc gagcataag 
acaaaggtc aggtcaatt tgtcttact caagacagc 
tgtagagct gtcattggg ggtgcagac ggaaaagtt 
tacatttac gatattcat agtgccaaa ttaattaaa 
tgcttgacg ggacagaac ggtgaagtt agcgccgtt 
cgggtgact gaaaaagac gattttctt ttaaccgcc 
ggtggaaat cgtgttata ttttatccg tttcgaagc 
gacgatcac attaagagt ttttcaaag atgaagaag 
aagtcgcag catcattta caagcccat ggcggcttc 
ttaacgtgt ctggacatt tcacgagac gggcaatta 
tcagtgaca ggtgcactg gaccatatt gtgaatgtc 
tggcagctt aatagtcaa gagttagta ttgactctg 
aagggacat tcgggtccc attacggcg gttagtttc 
gctgcaaat ggattattt gtggcgtct ggttccgaa 
gataaaaca gtcaaagtt tggggtcta actcttggt 
acattagtc tcgacgttt acgggtcac caagctgcc 
gtttcgaca gtatttgtt atgatggat tcgactcgg 
ataatctcg agcgaccga aatgacact ctatgcatt 
tggctggca gataacgga aacctttta caaacatat 
cccggtcct agcaaatgt gttagagtt acaaataat 
atgaagtac gcaatagca acaaatgga gacgtctcg 
ttgaaaatt tggtcttta gttaaagac gacgagaag 
tataatgtc aaccattcc gaaaaaata acttgcttt 
gttctaaca atagactct caacacatt atcacaggg 
tcaagggat atgtcattg aaagtttgg caagtcgtc 
ggagggaaa ctctctcaa gtcttgatc ggccacacg 
gactcggta acttgcgtg gcagtttca gtttcggat 
aaaagtcaa gtgatttcg ggagcgtgc gataataac 
cttatagtc tgggatata aacaccggt gccgattta 
tacacactt tccgcgcat ttaagttac gttacttgc 
gttaaatta tcaggggat ggaacaata gcgatatca 
ggatccgac gacaaaagc ataatcatt tgggacaca 
aaaagaggc ttacaacta acttcgctc caattacat 
tacccgatc ataagcatc gaacccaca tcagacttt 
tcccgaatt tcggtctta ttgaaagac actaacttc 
atgccaatt atttgcttg cacaacact ccggcaaag 
tacgtcaaa ctcccaact tattgcgca cccgacaag 
gatataata gaaaatccc aaaccggcc ccgaaacgg 
caaatgaag agattatta aagaaggaa gtttcttta 
gatacgtac acttggcaa aagaaatac gctcatctc 
acttcgaat ttggtaatt ccggctgtc gatgagcgg 
ttcaagcgc cgattttcg gtatccgcg agtatggaa 
gaaatctcg aaaatccca caaattaaa gactcgcag 
ggtggcttg ggagcgaaa caagcttct ctagcgcaa 
tctcagcat tttgatcaa ttggaagct ttgtggaat 
aaacgttcg ccaccgcga cggagactt catcagtcc 
ctatccaaa caatcatct ttggccgaa tcgcatctc 
gattcgtcg gacgaagat ggacatctc gaagacggt 
aaatatgcc acgtaa
    mRNA sequence
    • >TC004796 RA
      cagtatcga aaagcagcg tcgtgaagc gtcatcgtt 
cgcctctct agccccagg tcgttgtgc atttcagat 
tgcgatggc atcaaaatg agatgattt agataatag 
gaatttacc actgacaat cgattaatg tatctaata 
gtttattgt tgaaataat aagtgtgct tagtcgtta 
aaatcatga agaataacg atgagacaa ttctcgaaa 
ttcttcaag gacacaaaa cttctaaat ggccggctg 
cgaaactcg ttaaaattt tcattgcca gcactaaag 
cagattttg ttgaagagc gacgtcttt tgcttgaat 
atgttggtc cagaacttc aaacggtat tcgatgaac 
aaaagattg aggtcgaac tagtcgata tgtttttcg 
gagcgtctg aaaaggata ccttcgacg cgaaattat 
tcgaggatc atttaaacg aaataagaa tgtgccata 
agttatcac ggggatgtt tctttttga gtttagttg 
ggaataaat acgaacctc ttatagttc ctcctcgtt 
taaaatcgg aaaattttg aagccttag tcgacaaag 
taggagaat tggggctca attgtgata tattagaaa 
actggtact tcaaggaag gaaacgaat atatattaa 
aagactgca gattctcaa ctttagaag aatggagag 
ctatatcag aagaattaa tcactataa ttcacgaat 
gtttgcaaa acatgcctg caacaacaa agaaaaaat 
ttagctcgt atttaaaat cagttgtcg aacaacagt 
ttaattttg ctttagggt tggcacccg gcactgctc 
atcacgtgt taaacattt ttagagaat gggacgtac 
ctgaagaga ctgaaaaat ttcatcagg aaaattcta 
gccatgtta ataaaacta tagaaaact attgtcgtg 
aacaaataa agttgtttc attcgaata taaaattac 
ttattccag aagaaaata atcttaatt tcaaagtgc 
cttggaaac ctgggggaa taaatacga tacatgcag 
aacacgaag catatctaa acgaattta aaagtcgtg 
ttttaaata aattaaaag atttaatta gacaaagtt 
tggacgatg aaccggaat tgaaatcta aaaagaaga 
tcgtcgagg agaactttc aagaaaata taactcact 
taactcttt gttatgacg atatcaaag tagattttt 
ccaattcgg aaatcttgg agcgagtta aacagatag 
tacaaaaca ctacaaatg ttagacata atccaattt 
tgatatacg gcaaccctg gttcgggaa aaacctcgc 
ttatacaaa caatataca gaaacttcg aaaagtggt 
ttacatgca gaactctta gaataataa ggtttactt 
caacgaccc ctcgatcct catatattt tagagctgt 
taagagtca tatgccaac aaatttgca tagcattaa 
agcttccag aaggtttct tgccaaaag acgcttcct 
tcgatcctt tgtatatta acaactggt ttcaaagtt 
tactcagaa atttcgagg atatgaatc aaatccttg 
ttatattca tcgacgatt tacatttac tcaatccgt 
tagacgccg acgccgtta atgccctca gctggatac 
caataactc tacccaaaa gggtccaca tgatatgca 
caacttcca cagaactgg acaaactca gaatgacgc 
cagtgcaaa aagaaaggt tcaaaaacc cagactcct 
acctcgaat tacccgcag ttgatccat tcgacgact 
acgtgaata agaaatttg acagtttgg aggagtttt 
ttacgaaaa acgcggtgg caaagatat cctcgttga 
taagttgtt cggagtacg gtctgactg agactgaac 
ttttggaaa ttttaatgc cgacgagta atagcgagg 
cagtaattc atgtcgaag acggtaatt ttaattttt 
cttccctgt gctccatcc ggcgaaagt tggaacctc 
tccttaggg agaaaataa tgagcggga aattactga 
tcgaatgga gtcacgtga cgattaaag agattgtgc 
ggaagcgtt atctccaca atcaggaaa ctttaagga 
gcacacatt cggaattag ctaacttat ttttctacg 
agttttgca gcgatgaaa gctgcgacg aagaggacg 
aagaagggg agccagcac cgcttttat ccgaaatta 
gggaaactc catttcagt cgacggttt ataaagacg 
ttacgtaca gtcaaagac atatcgagg aagcttgga 
tccatcttt tgctcgctg gtgatacaa ttaaactga 
aaagcttgt gtatgtgca attacgact tccttcttg 
ccgctttgc aaacgtttt cggttagct acttgcgat 
gcttgctgg agcatgtcc gatgttatc ttcttgata 
gagagatcg aattggttt actactcta ttaggaagt 
ccacggatg tattaactc gggatactt atcaattag 
gcactcagc ttatctctt ggttgaggc cggtcatag 
aacgtggtg gtggtttga tgagctcta tggtcactt 
ctgccatgg cttggtgcg atggtttca ctttacctc 
ttgtggttc cattaacag actggcttc aaccacctc 
ttccacaac aatcccgcg tcattaaca caccgaatg 
tgcgactta tcgaaccca ctccttccg gacaacatg 
ttgtttgtg ttgtcgatt ccgagccgc agctttggc 
acataatga gcaaccaat tagtgcaca cctttaaag 
gtcatacgg ggaaaataa tttgtatgg ctgtgacca 
aacattccc agttcttgt ttaccggtt cggatgatt 
taagtataa tcgtttggg acttgaaat cattcggta 
ttaaattac gaattcatg aacacatcg caccggttt 
tgtgcatta ccggcgctc tcaataact cggtcatta 
tcagtggag gagaggaca gtagcatca ttataactt 
ctctggcag atggacacg tcgttatga aaatagacc 
accatcgag gccccgtaa cttccgtca aagtcactt 
cgacgggtg acatcttag tttcgggaa gtcatgatg 
gaagagtct gtttgtggt ctttggaaa cttacagtt 
tgcttaata cgattacgc tacaatcgc cgatacaaa 
tgatcgaag tttcggccg attcggtct ttctattag 
cttgttgta gggacaaca atctctatt tacgcacat 
tggcgacgg gaaccgaac tacacagtt tagtcgagc 
ataagacaa aggtcaggt caatttgtc ttactcaag 
acagctgta gagctgtca ttgggggtg cagacggaa 
aagtttaca tttacgata ttcatagtg ccaaattaa 
ttaaatgct tgacgggac agaacggtg aagttagcg 
ccgttcggg tgactgaaa aagacgatt ttcttttaa 
ccgccggtg gaaatcgtg ttatatttt atccgtttc 
gaagcgacg atcacatta agagttttt caaagatga 
agaagaagt cgcagcatc atttacaag cccatggcg 
gcttcttaa cgtgtctgg acatttcac gagacgggc 
aattatcag tgacaggtg cactggacc atattgtga 
atgtctggc agcttaata gtcaagagt tagtattga 
ctctgaagg gacattcgg gtcccatta cggcggtta 
gtttcgctg caaatggat tatttgtgg cgtctggtt 
ccgaagata aaacagtca aagtttggg gtctaactc 
ttggtacat tagtctcga cgtttacgg gtcaccaag 
ctgccgttt cgacagtat ttgttatga tggattcga 
ctcggataa tctcgagcg accgaaatg acactctat 
gcatttggc tggcagata acggaaacc ttttacaaa 
catatcccg gtcctagca aatgtgtta gagttacaa 
ataatatga agtacgcaa tagcaacaa atggagacg 
tctcgttga aaatttggt ctttagtta aagacgacg 
agaagtata atgtcaacc attccgaaa aaataactt 
gctttgttc taacaatag actctcaac acattatca 
cagggtcaa gggatatgt cattgaaag tttggcaag 
tcgtcggag ggaaactct ctcaagtct tgatcggcc 
acacggact cggtaactt gcgtggcag tttcagttt 
cggataaaa gtcaagtga tttcgggag cgtgcgata 
ataacctta tagtctggg atataaaca ccggtgccg 
atttataca cactttccg cgcatttaa gttacgtta 
cttgcgtta aattatcag gggatggaa caatagcga 
tatcaggat ccgacgaca aaagcataa tcatttggg 
acacaaaaa gaggcttac aactaactt cgctccaat 
tacattacc cgatcataa gcatcgaac ccacatcag 
acttttccc gaatttcgg tcttattga aagacacta 
acttcatgc caattattt gcttgcaca acactccgg 
caaagtacg tcaaactcc caacttatt gcgcacccg 
acaaggata taatagaaa atcccaaac cggccccga 
aacggcaaa tgaagagat tattaaaga aggaagttt 
ctttagata cgtacactt ggcaaaaga aatacgctc 
atctcactt cgaatttgg taattccgg ctgtcgatg 
agcggttca agcgccgat tttcggtat ccgcgagta 
tggaagaaa tctcgaaaa tcccacaaa ttaaagact 
cgcagggtg gcttgggag cgaaacaag cttctctag 
cgcaatctc agcattttg atcaattgg aagctttgt 
ggaataaac gttcgccac cgcgacgga gacttcatc 
agtccctat ccaaacaat catctttgg ccgaatcgc 
atctcgatt cgtcggacg aagatggac atctcgaag 
acggtaaat atgccacgt aatttgttt ttagaaaat 
cacttgaaa aactcaaaa aaaatcgat gtcgtattt 
ggtcaaacc ttcccgcaa atggatgtt gtaaatgta 
gtacttcca cgaaatgtt aaacctata ttatactat 
atagaataa aagtaaaat gcaaatgat aatctatat 
gacataagt gctttcctt atgactgtt tcaaatttt 
tagactttc taaaatatt attttatat aattgctat 
ttactctag taataagaa aaaatttca tattatcaa 
ctgtacctt attattgtt tcttgttac tgactattg 
aaagcaatc attccacac attgcaaaa atgtttgtt 
gacatgtta acgtttgtt attttaaat ttgagaact 
ttactcgaa gaaacaacg aaaattgta atagaattg 
atcggtagg tatacgaaa ttaatccat attcgcaaa 
tgacgcacc attatttta actaaagtt ctcaaatta 
accaatact ctatttcga caatggaaa ttccaactc 
tccttttgg cgattattt tagtccatc tgttatagc 
ggaaaagaa ttctcagga ccaaaaatt ttatttaaa 
taatatata gatttttta ttataaatt tgtattttt 
ataaaataa ctcagttat cataacccg attaaagta 
aaaactctg gcttaacac gtaacgacg ctcaataaa 
taaaatagt tcacatttt agcgtacag gtgtataat 
ttgaaagtc agacgccat aaaaacgtc aaaaatgtc 
gtttctatc gcgcttgac tttgatgtt tcttccgac 
acgcttcac tcttcagtg ttcatattt taaatgtat 
tcgtctata ttccgatta ctctctatt gctgtttac 
tgcattatg tgaccttac gtataaacg ataaattgt 
tacgtttgt ttgtcgtac ttatttcaa tgttagtca 
ttgttacct aaagcaatt ctaaatctc gctacctaa 
gcaataatg taacttatt aaaaaacca ccgtttaga 
aaaatacaa atcacaaat atataaaat gctatgtta 
atatgtgta atgaattag aaaaatgag tcgaaaata 
aaactttga gaaacgt
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