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Gene information
General information
TC004796 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7320500..7339104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004796
    iBeetle screen iB_03849

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    gnathal appendages randomremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004796 RA
      MKNNDETIL EILQGHKTS KWPAAKLVK IFIASTKAD 
      FVEERRLLL EYVGPELQT VFDEQKIEV ELVDMFFGA 
      SEKDTFDAK LFEDHLNEI RMCHKLSRG CFFLSLVGN 
      KYEPLIVPP RLKSENFEA LVDKVGELG LNCDILENW 
      YFKEGNEYI LKDCRFSTL EEWRAISEE LITIIHECL 
      QNMPATTKK KFSSYLKSV VEQQFNFAL GLAPGTAHH 
      VLNIFREWD VPEETEKFH QENSSHVNK TIENYCREQ 
      IKLFHSNIK LLIPEENNL NFKVPWKPG GINTIHAEH 
      EAYLNEFKS RVLNKLKDL IRQSLDDEP ELKSKKKIV 
      EENFQENIT HLTLCYDDI KVDFSNSEI LERVKQIVQ 
      NTTNVRHNP ILIYGNPGS GKTSLIQTI YRNFEKWFT 
      CRTLRIIRF TSTTPRSSY ILELLRVIC QQICIALKL 
      PEGFLPKDA SFDPLYINN WFQSLLRNF EDMNQILVI 
      FIDDLHLLN PLDADAVNA LSWIPITLP KRVHMICTT 
      STELDKLRM TPVQKERFK NPDSYLELP AVDPFDDYV 
      NKKFDSLEE FFTKNAVAK ISSLISCSE YGLTETELL 
      EILMPTSNS EAVIHVEDG NFNFSSLCS IRRKLEPLL 
      REKIMSGKL LIEWSHVTI KEIVRKRYL HNQETLRST 
      HSELANLFF YEFCSDESC DEEDEEGEP APLLSEIRE 
      TPFQSTVYK DVTYSQRHI EEAWIHLLL AGDTIKLKS 
      LCMCNYDFL LAALQTFSV SYLRCLLEH VRCYLLDRE 
      IELVYYSIR KSTDVLTRD TYQLGTQLI SWLRPVIER 
      GGGLMSSMV TSAMAWCDG FTLPLVVPL TDWLQPPLP 
      QQSRVINTP NVRLIEPTP SGQHVVCVV DSEPQLWHI 
      MSNQLVHTF KGHTGKIIC MAVTKHSQF LFTGSDDLS 
      IIVWDLKSF GIKLRIHEH IAPVLCITG ALNNSVIIS 
      GGEDSSIII TSLADGHVV MKIDHHRGP VTSVKVTST 
      GDILVSGSH DGRVCLWSL ETYSLLNTI TLQSPIQMI 
      EVSADSVFL LACCRDNNL YLRTLATGT ELHSLVEHK 
      TKVRSICLT QDSCRAVIG GADGKVYIY DIHSAKLIK 
      CLTGQNGEV SAVRVTEKD DFLLTAGGN RVIFYPFRS 
      DDHIKSFSK MKKKSQHHL QAHGGFLTC LDISRDGQL 
      SVTGALDHI VNVWQLNSQ ELVLTLKGH SGPITAVSF 
      AANGLFVAS GSEDKTVKV WGLTLGTLV STFTGHQAA 
      VSTVFVMMD STRIISSDR NDTLCIWLA DNGNLLQTY 
      PGPSKCVRV TNNMKYAIA TNGDVSLKI WSLVKDDEK 
      YNVNHSEKI TCFVLTIDS QHIITGSRD MSLKVWQVV 
      GGKLSQVLI GHTDSVTCV AVSVSDKSQ VISGACDNN 
      LIVWDINTG ADLYTLSAH LSYVTCVKL SGDGTIAIS 
      GSDDKSIII WDTKRGLQL TSLQLHYPI ISIEPTSDF 
      SRISVLLKD TNFMPIICL HNTPAKYVK LPTYCAPDK 
      DIIENPKPA PKRQMKRLL KKEVSLDTY TWQKKYAHL 
      TSNLVIPAV DERFKRRFS VSASMEEIS KIPQIKDSQ 
      GGLGAKQAS LAQSQHFDQ LEALWNKRS PPRRRLHQS 
      LSKQSSLAE SHLDSSDED GHLEDGKYA T
    CDS sequence
    • >TC004796 RA
      atgaagaat aacgatgag acaattctc gaaattctt 
      caaggacac aaaacttct aaatggccg gctgcgaaa 
      ctcgttaaa attttcatt gccagcact aaagcagat 
      tttgttgaa gagcgacgt cttttgctt gaatatgtt 
      ggtccagaa cttcaaacg gtattcgat gaacaaaag 
      attgaggtc gaactagtc gatatgttt ttcggagcg 
      tctgaaaag gataccttc gacgcgaaa ttattcgag 
      gatcattta aacgaaata agaatgtgc cataagtta 
      tcacgggga tgtttcttt ttgagttta gttgggaat 
      aaatacgaa cctcttata gttcctcct cgtttaaaa 
      tcggaaaat tttgaagcc ttagtcgac aaagtagga 
      gaattgggg ctcaattgt gatatatta gaaaactgg 
      tacttcaag gaaggaaac gaatatata ttaaaagac 
      tgcagattc tcaacttta gaagaatgg agagctata 
      tcagaagaa ttaatcact ataattcac gaatgtttg 
      caaaacatg cctgcaaca acaaagaaa aaatttagc 
      tcgtattta aaatcagtt gtcgaacaa cagtttaat 
      tttgcttta gggttggca cccggcact gctcatcac 
      gtgttaaac atttttaga gaatgggac gtacctgaa 
      gagactgaa aaatttcat caggaaaat tctagccat 
      gttaataaa actatagaa aactattgt cgtgaacaa 
      ataaagttg tttcattcg aatataaaa ttacttatt 
      ccagaagaa aataatctt aatttcaaa gtgccttgg 
      aaacctggg ggaataaat acgatacat gcagaacac 
      gaagcatat ctaaacgaa tttaaaagt cgtgtttta 
      aataaatta aaagattta attagacaa agtttggac 
      gatgaaccg gaattgaaa tctaaaaag aagatcgtc 
      gaggagaac tttcaagaa aatataact cacttaact 
      ctttgttat gacgatatc aaagtagat ttttccaat 
      tcggaaatc ttggagcga gttaaacag atagtacaa 
      aacactaca aatgttaga cataatcca attttgata 
      tacggcaac cctggttcg ggaaaaacc tcgcttata 
      caaacaata tacagaaac ttcgaaaag tggtttaca 
      tgcagaact cttagaata ataaggttt acttcaacg 
      acccctcga tcctcatat attttagag ctgttaaga 
      gtcatatgc caacaaatt tgcatagca ttaaagctt 
      ccagaaggt ttcttgcca aaagacgct tccttcgat 
      cctttgtat attaacaac tggtttcaa agtttactc 
      agaaatttc gaggatatg aatcaaatc cttgttata 
      ttcatcgac gatttacat ttactcaat ccgttagac 
      gccgacgcc gttaatgcc ctcagctgg ataccaata 
      actctaccc aaaagggtc cacatgata tgcacaact 
      tccacagaa ctggacaaa ctcagaatg acgccagtg 
      caaaaagaa aggttcaaa aacccagac tcctacctc 
      gaattaccc gcagttgat ccattcgac gactacgtg 
      aataagaaa tttgacagt ttggaggag ttttttacg 
      aaaaacgcg gtggcaaag atatcctcg ttgataagt 
      tgttcggag tacggtctg actgagact gaacttttg 
      gaaatttta atgccgacg agtaatagc gaggcagta 
      attcatgtc gaagacggt aattttaat ttttcttcc 
      ctgtgctcc atccggcga aagttggaa cctctcctt 
      agggagaaa ataatgagc gggaaatta ctgatcgaa 
      tggagtcac gtgacgatt aaagagatt gtgcggaag 
      cgttatctc cacaatcag gaaacttta aggagcaca 
      cattcggaa ttagctaac ttatttttc tacgagttt 
      tgcagcgat gaaagctgc gacgaagag gacgaagaa 
      ggggagcca gcaccgctt ttatccgaa attagggaa 
      actccattt cagtcgacg gtttataaa gacgttacg 
      tacagtcaa agacatatc gaggaagct tggatccat 
      cttttgctc gctggtgat acaattaaa ctgaaaagc 
      ttgtgtatg tgcaattac gacttcctt cttgccgct 
      ttgcaaacg ttttcggtt agctacttg cgatgcttg 
      ctggagcat gtccgatgt tatcttctt gatagagag 
      atcgaattg gtttactac tctattagg aagtccacg 
      gatgtatta actcgggat acttatcaa ttaggcact 
      cagcttatc tcttggttg aggccggtc atagaacgt 
      ggtggtggt ttgatgagc tctatggtc acttctgcc 
      atggcttgg tgcgatggt ttcacttta cctcttgtg 
      gttccatta acagactgg cttcaacca cctcttcca 
      caacaatcc cgcgtcatt aacacaccg aatgtgcga 
      cttatcgaa cccactcct tccggacaa catgttgtt 
      tgtgttgtc gattccgag ccgcagctt tggcacata 
      atgagcaac caattagtg cacaccttt aaaggtcat 
      acggggaaa ataatttgt atggctgtg accaaacat 
      tcccagttc ttgtttacc ggttcggat gatttaagt 
      ataatcgtt tgggacttg aaatcattc ggtattaaa 
      ttacgaatt catgaacac atcgcaccg gttttgtgc 
      attaccggc gctctcaat aactcggtc attatcagt 
      ggaggagag gacagtagc atcattata acttctctg 
      gcagatgga cacgtcgtt atgaaaata gaccaccat 
      cgaggcccc gtaacttcc gtcaaagtc acttcgacg 
      ggtgacatc ttagtttcg ggaagtcat gatggaaga 
      gtctgtttg tggtctttg gaaacttac agtttgctt 
      aatacgatt acgctacaa tcgccgata caaatgatc 
      gaagtttcg gccgattcg gtctttcta ttagcttgt 
      tgtagggac aacaatctc tatttacgc acattggcg 
      acgggaacc gaactacac agtttagtc gagcataag 
      acaaaggtc aggtcaatt tgtcttact caagacagc 
      tgtagagct gtcattggg ggtgcagac ggaaaagtt 
      tacatttac gatattcat agtgccaaa ttaattaaa 
      tgcttgacg ggacagaac ggtgaagtt agcgccgtt 
      cgggtgact gaaaaagac gattttctt ttaaccgcc 
      ggtggaaat cgtgttata ttttatccg tttcgaagc 
      gacgatcac attaagagt ttttcaaag atgaagaag 
      aagtcgcag catcattta caagcccat ggcggcttc 
      ttaacgtgt ctggacatt tcacgagac gggcaatta 
      tcagtgaca ggtgcactg gaccatatt gtgaatgtc 
      tggcagctt aatagtcaa gagttagta ttgactctg 
      aagggacat tcgggtccc attacggcg gttagtttc 
      gctgcaaat ggattattt gtggcgtct ggttccgaa 
      gataaaaca gtcaaagtt tggggtcta actcttggt 
      acattagtc tcgacgttt acgggtcac caagctgcc 
      gtttcgaca gtatttgtt atgatggat tcgactcgg 
      ataatctcg agcgaccga aatgacact ctatgcatt 
      tggctggca gataacgga aacctttta caaacatat 
      cccggtcct agcaaatgt gttagagtt acaaataat 
      atgaagtac gcaatagca acaaatgga gacgtctcg 
      ttgaaaatt tggtcttta gttaaagac gacgagaag 
      tataatgtc aaccattcc gaaaaaata acttgcttt 
      gttctaaca atagactct caacacatt atcacaggg 
      tcaagggat atgtcattg aaagtttgg caagtcgtc 
      ggagggaaa ctctctcaa gtcttgatc ggccacacg 
      gactcggta acttgcgtg gcagtttca gtttcggat 
      aaaagtcaa gtgatttcg ggagcgtgc gataataac 
      cttatagtc tgggatata aacaccggt gccgattta 
      tacacactt tccgcgcat ttaagttac gttacttgc 
      gttaaatta tcaggggat ggaacaata gcgatatca 
      ggatccgac gacaaaagc ataatcatt tgggacaca 
      aaaagaggc ttacaacta acttcgctc caattacat 
      tacccgatc ataagcatc gaacccaca tcagacttt 
      tcccgaatt tcggtctta ttgaaagac actaacttc 
      atgccaatt atttgcttg cacaacact ccggcaaag 
      tacgtcaaa ctcccaact tattgcgca cccgacaag 
      gatataata gaaaatccc aaaccggcc ccgaaacgg 
      caaatgaag agattatta aagaaggaa gtttcttta 
      gatacgtac acttggcaa aagaaatac gctcatctc 
      acttcgaat ttggtaatt ccggctgtc gatgagcgg 
      ttcaagcgc cgattttcg gtatccgcg agtatggaa 
      gaaatctcg aaaatccca caaattaaa gactcgcag 
      ggtggcttg ggagcgaaa caagcttct ctagcgcaa 
      tctcagcat tttgatcaa ttggaagct ttgtggaat 
      aaacgttcg ccaccgcga cggagactt catcagtcc 
      ctatccaaa caatcatct ttggccgaa tcgcatctc 
      gattcgtcg gacgaagat ggacatctc gaagacggt 
      aaatatgcc acgtaa
    mRNA sequence
    • >TC004796 RA
      cagtatcga aaagcagcg tcgtgaagc gtcatcgtt 
      cgcctctct agccccagg tcgttgtgc atttcagat 
      tgcgatggc atcaaaatg agatgattt agataatag 
      gaatttacc actgacaat cgattaatg tatctaata 
      gtttattgt tgaaataat aagtgtgct tagtcgtta 
      aaatcatga agaataacg atgagacaa ttctcgaaa 
      ttcttcaag gacacaaaa cttctaaat ggccggctg 
      cgaaactcg ttaaaattt tcattgcca gcactaaag 
      cagattttg ttgaagagc gacgtcttt tgcttgaat 
      atgttggtc cagaacttc aaacggtat tcgatgaac 
      aaaagattg aggtcgaac tagtcgata tgtttttcg 
      gagcgtctg aaaaggata ccttcgacg cgaaattat 
      tcgaggatc atttaaacg aaataagaa tgtgccata 
      agttatcac ggggatgtt tctttttga gtttagttg 
      ggaataaat acgaacctc ttatagttc ctcctcgtt 
      taaaatcgg aaaattttg aagccttag tcgacaaag 
      taggagaat tggggctca attgtgata tattagaaa 
      actggtact tcaaggaag gaaacgaat atatattaa 
      aagactgca gattctcaa ctttagaag aatggagag 
      ctatatcag aagaattaa tcactataa ttcacgaat 
      gtttgcaaa acatgcctg caacaacaa agaaaaaat 
      ttagctcgt atttaaaat cagttgtcg aacaacagt 
      ttaattttg ctttagggt tggcacccg gcactgctc 
      atcacgtgt taaacattt ttagagaat gggacgtac 
      ctgaagaga ctgaaaaat ttcatcagg aaaattcta 
      gccatgtta ataaaacta tagaaaact attgtcgtg 
      aacaaataa agttgtttc attcgaata taaaattac 
      ttattccag aagaaaata atcttaatt tcaaagtgc 
      cttggaaac ctgggggaa taaatacga tacatgcag 
      aacacgaag catatctaa acgaattta aaagtcgtg 
      ttttaaata aattaaaag atttaatta gacaaagtt 
      tggacgatg aaccggaat tgaaatcta aaaagaaga 
      tcgtcgagg agaactttc aagaaaata taactcact 
      taactcttt gttatgacg atatcaaag tagattttt 
      ccaattcgg aaatcttgg agcgagtta aacagatag 
      tacaaaaca ctacaaatg ttagacata atccaattt 
      tgatatacg gcaaccctg gttcgggaa aaacctcgc 
      ttatacaaa caatataca gaaacttcg aaaagtggt 
      ttacatgca gaactctta gaataataa ggtttactt 
      caacgaccc ctcgatcct catatattt tagagctgt 
      taagagtca tatgccaac aaatttgca tagcattaa 
      agcttccag aaggtttct tgccaaaag acgcttcct 
      tcgatcctt tgtatatta acaactggt ttcaaagtt 
      tactcagaa atttcgagg atatgaatc aaatccttg 
      ttatattca tcgacgatt tacatttac tcaatccgt 
      tagacgccg acgccgtta atgccctca gctggatac 
      caataactc tacccaaaa gggtccaca tgatatgca 
      caacttcca cagaactgg acaaactca gaatgacgc 
      cagtgcaaa aagaaaggt tcaaaaacc cagactcct 
      acctcgaat tacccgcag ttgatccat tcgacgact 
      acgtgaata agaaatttg acagtttgg aggagtttt 
      ttacgaaaa acgcggtgg caaagatat cctcgttga 
      taagttgtt cggagtacg gtctgactg agactgaac 
      ttttggaaa ttttaatgc cgacgagta atagcgagg 
      cagtaattc atgtcgaag acggtaatt ttaattttt 
      cttccctgt gctccatcc ggcgaaagt tggaacctc 
      tccttaggg agaaaataa tgagcggga aattactga 
      tcgaatgga gtcacgtga cgattaaag agattgtgc 
      ggaagcgtt atctccaca atcaggaaa ctttaagga 
      gcacacatt cggaattag ctaacttat ttttctacg 
      agttttgca gcgatgaaa gctgcgacg aagaggacg 
      aagaagggg agccagcac cgcttttat ccgaaatta 
      gggaaactc catttcagt cgacggttt ataaagacg 
      ttacgtaca gtcaaagac atatcgagg aagcttgga 
      tccatcttt tgctcgctg gtgatacaa ttaaactga 
      aaagcttgt gtatgtgca attacgact tccttcttg 
      ccgctttgc aaacgtttt cggttagct acttgcgat 
      gcttgctgg agcatgtcc gatgttatc ttcttgata 
      gagagatcg aattggttt actactcta ttaggaagt 
      ccacggatg tattaactc gggatactt atcaattag 
      gcactcagc ttatctctt ggttgaggc cggtcatag 
      aacgtggtg gtggtttga tgagctcta tggtcactt 
      ctgccatgg cttggtgcg atggtttca ctttacctc 
      ttgtggttc cattaacag actggcttc aaccacctc 
      ttccacaac aatcccgcg tcattaaca caccgaatg 
      tgcgactta tcgaaccca ctccttccg gacaacatg 
      ttgtttgtg ttgtcgatt ccgagccgc agctttggc 
      acataatga gcaaccaat tagtgcaca cctttaaag 
      gtcatacgg ggaaaataa tttgtatgg ctgtgacca 
      aacattccc agttcttgt ttaccggtt cggatgatt 
      taagtataa tcgtttggg acttgaaat cattcggta 
      ttaaattac gaattcatg aacacatcg caccggttt 
      tgtgcatta ccggcgctc tcaataact cggtcatta 
      tcagtggag gagaggaca gtagcatca ttataactt 
      ctctggcag atggacacg tcgttatga aaatagacc 
      accatcgag gccccgtaa cttccgtca aagtcactt 
      cgacgggtg acatcttag tttcgggaa gtcatgatg 
      gaagagtct gtttgtggt ctttggaaa cttacagtt 
      tgcttaata cgattacgc tacaatcgc cgatacaaa 
      tgatcgaag tttcggccg attcggtct ttctattag 
      cttgttgta gggacaaca atctctatt tacgcacat 
      tggcgacgg gaaccgaac tacacagtt tagtcgagc 
      ataagacaa aggtcaggt caatttgtc ttactcaag 
      acagctgta gagctgtca ttgggggtg cagacggaa 
      aagtttaca tttacgata ttcatagtg ccaaattaa 
      ttaaatgct tgacgggac agaacggtg aagttagcg 
      ccgttcggg tgactgaaa aagacgatt ttcttttaa 
      ccgccggtg gaaatcgtg ttatatttt atccgtttc 
      gaagcgacg atcacatta agagttttt caaagatga 
      agaagaagt cgcagcatc atttacaag cccatggcg 
      gcttcttaa cgtgtctgg acatttcac gagacgggc 
      aattatcag tgacaggtg cactggacc atattgtga 
      atgtctggc agcttaata gtcaagagt tagtattga 
      ctctgaagg gacattcgg gtcccatta cggcggtta 
      gtttcgctg caaatggat tatttgtgg cgtctggtt 
      ccgaagata aaacagtca aagtttggg gtctaactc 
      ttggtacat tagtctcga cgtttacgg gtcaccaag 
      ctgccgttt cgacagtat ttgttatga tggattcga 
      ctcggataa tctcgagcg accgaaatg acactctat 
      gcatttggc tggcagata acggaaacc ttttacaaa 
      catatcccg gtcctagca aatgtgtta gagttacaa 
      ataatatga agtacgcaa tagcaacaa atggagacg 
      tctcgttga aaatttggt ctttagtta aagacgacg 
      agaagtata atgtcaacc attccgaaa aaataactt 
      gctttgttc taacaatag actctcaac acattatca 
      cagggtcaa gggatatgt cattgaaag tttggcaag 
      tcgtcggag ggaaactct ctcaagtct tgatcggcc 
      acacggact cggtaactt gcgtggcag tttcagttt 
      cggataaaa gtcaagtga tttcgggag cgtgcgata 
      ataacctta tagtctggg atataaaca ccggtgccg 
      atttataca cactttccg cgcatttaa gttacgtta 
      cttgcgtta aattatcag gggatggaa caatagcga 
      tatcaggat ccgacgaca aaagcataa tcatttggg 
      acacaaaaa gaggcttac aactaactt cgctccaat 
      tacattacc cgatcataa gcatcgaac ccacatcag 
      acttttccc gaatttcgg tcttattga aagacacta 
      acttcatgc caattattt gcttgcaca acactccgg 
      caaagtacg tcaaactcc caacttatt gcgcacccg 
      acaaggata taatagaaa atcccaaac cggccccga 
      aacggcaaa tgaagagat tattaaaga aggaagttt 
      ctttagata cgtacactt ggcaaaaga aatacgctc 
      atctcactt cgaatttgg taattccgg ctgtcgatg 
      agcggttca agcgccgat tttcggtat ccgcgagta 
      tggaagaaa tctcgaaaa tcccacaaa ttaaagact 
      cgcagggtg gcttgggag cgaaacaag cttctctag 
      cgcaatctc agcattttg atcaattgg aagctttgt 
      ggaataaac gttcgccac cgcgacgga gacttcatc 
      agtccctat ccaaacaat catctttgg ccgaatcgc 
      atctcgatt cgtcggacg aagatggac atctcgaag 
      acggtaaat atgccacgt aatttgttt ttagaaaat 
      cacttgaaa aactcaaaa aaaatcgat gtcgtattt 
      ggtcaaacc ttcccgcaa atggatgtt gtaaatgta 
      gtacttcca cgaaatgtt aaacctata ttatactat 
      atagaataa aagtaaaat gcaaatgat aatctatat 
      gacataagt gctttcctt atgactgtt tcaaatttt 
      tagactttc taaaatatt attttatat aattgctat 
      ttactctag taataagaa aaaatttca tattatcaa 
      ctgtacctt attattgtt tcttgttac tgactattg 
      aaagcaatc attccacac attgcaaaa atgtttgtt 
      gacatgtta acgtttgtt attttaaat ttgagaact 
      ttactcgaa gaaacaacg aaaattgta atagaattg 
      atcggtagg tatacgaaa ttaatccat attcgcaaa 
      tgacgcacc attatttta actaaagtt ctcaaatta 
      accaatact ctatttcga caatggaaa ttccaactc 
      tccttttgg cgattattt tagtccatc tgttatagc 
      ggaaaagaa ttctcagga ccaaaaatt ttatttaaa 
      taatatata gatttttta ttataaatt tgtattttt 
      ataaaataa ctcagttat cataacccg attaaagta 
      aaaactctg gcttaacac gtaacgacg ctcaataaa 
      taaaatagt tcacatttt agcgtacag gtgtataat 
      ttgaaagtc agacgccat aaaaacgtc aaaaatgtc 
      gtttctatc gcgcttgac tttgatgtt tcttccgac 
      acgcttcac tcttcagtg ttcatattt taaatgtat 
      tcgtctata ttccgatta ctctctatt gctgtttac 
      tgcattatg tgaccttac gtataaacg ataaattgt 
      tacgtttgt ttgtcgtac ttatttcaa tgttagtca 
      ttgttacct aaagcaatt ctaaatctc gctacctaa 
      gcaataatg taacttatt aaaaaacca ccgtttaga 
      aaaatacaa atcacaaat atataaaat gctatgtta 
      atatgtgta atgaattag aaaaatgag tcgaaaata 
      aaactttga gaaacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY