Status
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Gene information
General information
TC004793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7275314..7297026
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004793
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03848

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004793 RA
      MTGASTGAT STGGGDAST SHCKGHRRQ ESMYAMTGL 
      YSESVPEDN GTADTKCST TFCHDTVIK CHSRNPSAG 
      FDRDKTAHA TTYSDPPPV ILRDTKECG ILKCRPSFI 
      QKYAGIKCF VFLLSFLVT LQQALSSGY INSVITTIE 
      KRFEIPSSL SGLIASSYE MGNVITVIF VSYLGSRRH 
      IPVWIGVGA VIMGVGSLI FMVPHFIGD ENVGEVVIN 
      NTDENMCRV ISVHEEDMG LGRLSSGLS SPPLAPNNL 
      RGDNCIQGS PSTTGPVLL FVLAQLLLG CGGSPLFTL 
      GTTYVDDHV RPESSSIYI GCMYSMAAF GPVLGFLLG 
      AYLLSFHMD SFSKIISID AGDSKWVGM WWGGFLLGG 
      LLLIFVSVP FFSFPKVLT HEKEKIRLT EKAAAAGSS 
      NATAANQQH AAPMTKNDS GYGKDIKDI PRSMWRLAC 
      NPIYIVTCL GACMELIIV SGFVVFLPK YLETQFSLG 
      KSQASVFTG SIAIPGACI GIFMGGCIL KRMELRPKG 
      AVQFVLISN MICLICYVL LFFLGCDNL KMAGTTIPY 
      FNSTHAEPF QVNLTSSCN FGCECSMND VEPVCGNNG 
      LTYFSPCHA GCTSTLRSD YTNCACIHG NMSLSGTTS 
      SVNLAARTN SEYAEVTVV PVATAGPCN SSCHTIYPF 
      LILLFFMTF IVAITQMPL LMIVLRSVS EEERSFALG 
      MQFVIFRLF GYIPAPILF GNLIDSTCL LWKSTCGSK 
      GGRCLLYDI EQFRYRYVG LCAGIKILA LGIFLVDWW 
      LVRRRKHLD EAGGITTNE IVGSIISLD KLFEEKPHF 
      EAGHRRNLS SVSTYDCPT SPKEENETK NELLTGTDA 
      NPTQEAGEE LEQSRPQTD LS
    CDS sequence
    • >TC004793 RA
      atgacgggt gcaagcacg ggtgccacc tccacgggt 
      gggggcgac gcctccacc agccactgc aagggccat 
      cgccggcag gagtccatg tatgccatg acaggactt 
      tactccgaa agcgtccct gaagacaat ggcacagct 
      gacacaaaa tgttcgacg acgttttgt catgacact 
      gtcataaag tgtcacagt cgtaaccct tcggccggt 
      tttgataga gacaaaacc gcacatgcc actacatac 
      tctgaccca ccacctgtg atattaagg gatacaaaa 
      gaatgcgga atccttaag tgccgaccc tcctttatt 
      caaaaatat gcaggaatt aagtgtttt gtttttctt 
      ttatcattc ctcgttacg ttgcaacaa gcgttaagc 
      tccggctat atcaattct gtgataaca accattgaa 
      aagcgtttt gagattcca tcgagtctt tcaggatta 
      attgcatca tcgtacgaa atgggcaat gtgataact 
      gtaatattt gttagttat ttggggagt cggcgacat 
      atcccggtg tggatcggg gtgggggct gttataatg 
      ggggtgggc tcgttgatc ttcatggta ccgcatttt 
      atcggcgac gagaacgtc ggtgaagtg gtcataaac 
      aatacagac gagaatatg tgtcgagtt atttcggtc 
      catgaggaa gacatgggc ttagggagg ctctcctcc 
      ggcctgtct tcgccacct ttagccccg aataatctg 
      cggggggat aattgcatt cagggttct ccgtcaacg 
      actggacca gtattgtta tttgtactt gcacaattg 
      ttattaggc tgtggaggt tcgccatta tttactttg 
      ggaacaact tatgtcgat gatcatgtc agacccgaa 
      agttcttct atttatata ggctgtatg tacagcatg 
      gccgcgttt ggtccagtc ttgggcttc ttactgggg 
      gcgtatttg ctctcgttc catatggat tccttttcg 
      aaaataatt tcgattgat gctggtgac agcaaatgg 
      gtgggtatg tggtggggt ggttttctc ttaggagga 
      cttttacta attttcgtg tcagtcccg tttttctcc 
      tttcctaag gttctgaca cacgaaaag gagaaaatt 
      cggttgacg gaaaaagct gcagctgct ggtagcagt 
      aatgctacg gcggcaaac caacaacac gcagcgcca 
      atgacgaaa aatgattca gggtatggc aaagacatt 
      aaagatatt cctcgttcg atgtggcgt cttgcttgt 
      aatccgatt tatatagtt acatgttta ggagcatgt 
      atggagcta ataattgta tcagggttc gttgtcttt 
      ttaccaaag tatctcgag acgcagttt agtttaggc 
      aaaagccaa gcgagtgtt ttcacagga tcaatcgcg 
      attcctggt gcatgtatt ggaattttt atgggaggc 
      tgcatttta aaacgaatg gaattacga cccaagggc 
      gccgttcag ttcgtccta atctcgaac atgatttgt 
      ttaatttgt tacgtgctg ttatttttc ctgggttgt 
      gataattta aaaatggct gggacaact attccttat 
      tttaacagc actcatgcc gaacctttc caagtgaat 
      ttgacgtcg tcctgcaat tttggatgc gagtgcagc 
      atgaacgat gtggagccg gtttgtggc aacaacggc 
      ttgacttat ttcagcccc tgtcacgct ggatgcacc 
      tcaacactc cgatcggat tataccaat tgtgcctgt 
      attcacgga aacatgagc ctgagtgga acaacttcg 
      tccgtaaat ctcgcagca cggacaaac agtgaatac 
      gctgaagtc actgttgtt cctgttgcg acagccggt 
      ccttgcaac tcatcctgt catacaatt tatccattt 
      ttgatactc ctcttcttt atgacattc atagtggcc 
      ataacacaa atgccgcta cttatgata gttttaaga 
      tccgtaagc gaggaagag cgctcattt gccttagga 
      atgcagttt gtgatattt cgcttgttt ggctacatc 
      ccagcccca attcttttc ggtaatttg atcgattcg 
      acttgtctt ctttggaag agcacgtgt ggctcgaaa 
      ggtggccgc tgtctcttg tacgatatc gagcaattc 
      cggtatagg tatgtgggg ctttgcgct gggattaaa 
      attcttgcc ttggggatt ttcctggtt gattggtgg 
      ctagtacgt cggaggaaa cacctcgac gaagctggt 
      gggatcacc accaatgaa atcgtcggc tcgattata 
      agcctagac aaattgttt gaggagaag ccgcacttt 
      gaggcggga catcgcaga aacttgagc tcggtctcg 
      acttacgat tgcccgact tcgccgaaa gaggagaat 
      gaaacgaaa aatgagtta cttacaggg acggatgcg 
      aatccgacc caggaagcg ggcgaagag ttggagcaa 
      agcaggcca caaaccgac ttatcttaa
    mRNA sequence
    • >TC004793 RA
      tgcacaagt gcacgacca gctgtgttg tctcaactg 
      tacctccgt gttgtgcgg caaataact tgtatataa 
      ataaatact cgaaaaaaa tcgacaaga tgtatcaat 
      taaaaactg tggttgtgt gttcagtgt gataaaccg 
      aataaaaat atttttgat acgggcctg gattatcga 
      gagagattt aaggatgca ttgacctcc cacaatttc 
      agatgccgc aatgggtga attgagttg atgcgagat 
      gacgggtgc aagcacggg tgccacctc cacgggtgg 
      gggcgacgc ctccaccag ccactgcaa gggccatcg 
      ccggcagga gtccatgta tgccatgac aggacttta 
      ctccgaaag cgtccctga agacaatgg cacagctga 
      cacaaaatg ttcgacgac gttttgtca tgacactgt 
      cataaagtg tcacagtcg taacccttc ggccggttt 
      tgatagaga caaaaccgc acatgccac tacatactc 
      tgacccacc acctgtgat attaaggga tacaaaaga 
      atgcggaat ccttaagtg ccgaccctc ctttattca 
      aaaatatgc aggaattaa gtgttttgt ttttctttt 
      atcattcct cgttacgtt gcaacaagc gttaagctc 
      cggctatat caattctgt gataacaac cattgaaaa 
      gcgttttga gattccatc gagtctttc aggattaat 
      tgcatcatc gtacgaaat gggcaatgt gataactgt 
      aatatttgt tagttattt ggggagtcg gcgacatat 
      cccggtgtg gatcggggt gggggctgt tataatggg 
      ggtgggctc gttgatctt catggtacc gcattttat 
      cggcgacga gaacgtcgg tgaagtggt cataaacaa 
      tacagacga gaatatgtg tcgagttat ttcggtcca 
      tgaggaaga catgggctt agggaggct ctcctccgg 
      cctgtcttc gccaccttt agccccgaa taatctgcg 
      gggggataa ttgcattca gggttctcc gtcaacgac 
      tggaccagt attgttatt tgtacttgc acaattgtt 
      attaggctg tggaggttc gccattatt tactttggg 
      aacaactta tgtcgatga tcatgtcag acccgaaag 
      ttcttctat ttatatagg ctgtatgta cagcatggc 
      cgcgtttgg tccagtctt gggcttctt actgggggc 
      gtatttgct ctcgttcca tatggattc cttttcgaa 
      aataatttc gattgatgc tggtgacag caaatgggt 
      gggtatgtg gtggggtgg ttttctctt aggaggact 
      tttactaat tttcgtgtc agtcccgtt tttctcctt 
      tcctaaggt tctgacaca cgaaaagga gaaaattcg 
      gttgacgga aaaagctgc agctgctgg tagcagtaa 
      tgctacggc ggcaaacca acaacacgc agcgccaat 
      gacgaaaaa tgattcagg gtatggcaa agacattaa 
      agatattcc tcgttcgat gtggcgtct tgcttgtaa 
      tccgattta tatagttac atgtttagg agcatgtat 
      ggagctaat aattgtatc agggttcgt tgtcttttt 
      accaaagta tctcgagac gcagtttag tttaggcaa 
      aagccaagc gagtgtttt cacaggatc aatcgcgat 
      tcctggtgc atgtattgg aatttttat gggaggctg 
      cattttaaa acgaatgga attacgacc caagggcgc 
      cgttcagtt cgtcctaat ctcgaacat gatttgttt 
      aatttgtta cgtgctgtt atttttcct gggttgtga 
      taatttaaa aatggctgg gacaactat tccttattt 
      taacagcac tcatgccga acctttcca agtgaattt 
      gacgtcgtc ctgcaattt tggatgcga gtgcagcat 
      gaacgatgt ggagccggt ttgtggcaa caacggctt 
      gacttattt cagcccctg tcacgctgg atgcacctc 
      aacactccg atcggatta taccaattg tgcctgtat 
      tcacggaaa catgagcct gagtggaac aacttcgtc 
      cgtaaatct cgcagcacg gacaaacag tgaatacgc 
      tgaagtcac tgttgttcc tgttgcgac agccggtcc 
      ttgcaactc atcctgtca tacaattta tccattttt 
      gatactcct cttctttat gacattcat agtggccat 
      aacacaaat gccgctact tatgatagt tttaagatc 
      cgtaagcga ggaagagcg ctcatttgc cttaggaat 
      gcagtttgt gatatttcg cttgtttgg ctacatccc 
      agccccaat tcttttcgg taatttgat cgattcgac 
      ttgtcttct ttggaagag cacgtgtgg ctcgaaagg 
      tggccgctg tctcttgta cgatatcga gcaattccg 
      gtataggta tgtggggct ttgcgctgg gattaaaat 
      tcttgcctt ggggatttt cctggttga ttggtggct 
      agtacgtcg gaggaaaca cctcgacga agctggtgg 
      gatcaccac caatgaaat cgtcggctc gattataag 
      cctagacaa attgtttga ggagaagcc gcactttga 
      ggcgggaca tcgcagaaa cttgagctc ggtctcgac 
      ttacgattg cccgacttc gccgaaaga ggagaatga 
      aacgaaaaa tgagttact tacagggac ggatgcgaa 
      tccgaccca ggaagcggg cgaagagtt ggagcaaag 
      caggccaca aaccgactt atcttaagt aacttatgc 
      acttacatc tttagtgat tcctgcctt aaaaacgta 
      gctaacgta ggcacttta ttttttact tttttattt 
      attaattac ttaatatta gtttaggag aaatgtgat 
      ctctcggtc aatttaaat gtaaataat gtttttatt 
      tcgatgacc cacattcca tttaataag ttgctaccg 
      actagctgt gtacttatt acacttagt ttaatttta 
      tctatattt aaatattgc aataaaaca gtgccacat 
      tattcaaat tctatgtat taaatgaag taataatca 
      attgccctt acggtgata cacacttgt agaatttgt 
      ttaaattca attgaatca tagaggcct atgttatat 
      gaatagtgc aatatatat atcttaaat gaattaaag 
      cacatctgt ggttttttg atactttgt tatgtaatt 
      gaacaagaa caaagtcta caatgctca agatttcga 
      atttttttc cgtatgatt gtaaggttt tgtcgcttc 
      aaaaattct attttgtca cgctgtatc catttttaa 
      tacaatttt taaaaggtt cttttgttt tatttagtc 
      tcattttca ttactgatt gtacatctc acatcagct 
      tgtgtaggt catgcgcat gctcctaac agttctcag 
      cacatggct atattaatt accgacaat caaacatat 
      ctatatcaa agttagagt tacgtttca gtcttgctt 
      ttagtctcg tgtgatcac gattgtact cttgtgatc 
      acacgaagt accgctgag attcagatt gttacccaa 
      gatttgtaa ggaatttta ttaaacgtt gttgacagt 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY