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Gene information
General information
TC004792 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 7006660..7019797
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004792
    iBeetle screen iB_03847
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 11.11% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    Comment: No phenotype found!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004792 RA
      MEDNNGAKS RARSNSARV LLFDDVGLP HDRTKPPPI 
      APGPPPKHA QPDHQSTPS NLVRISSAL GKSAPSLSV 
      NMKDVNVGR RNSRPAAVH RLSFVANTS PVLPRSQSP 
      ICGSPIDSP RTHNSFINF PFAPIKRIV AGCRGDGRR 
      WSVASLPSS GYGTTPGSS NMSSKCSSQ ERLHQLPHV 
      PTSDDMRIL THHFSSNES NPSLPTGNY EENPISHRS 
      PLHRPRSRS LSSPSRSPV MDNEICMMN VLYKERFPK 
      ATQQMEERL NHYIEENKT FDFGEDCDY LPIVRFVHH 
      QVLEMARDC LHKSQSKLI TSRYFYEMS ENLERLLTE 
      TKDKSEEAA NLVTGFIKK LLLIISRPA RLLECLEFD 
      PEEFYHFLE AAEGQVKVV QGIKADIPQ YIIQKLGLN 
      RDPIAELQE ELRDCNWSS NQSSRSSVC LTSTPSTKK 
      SLSNPNEQD FEVVKLISN GAYGAVYLV KHKQTRQRF 
      AMKKINKNN LILRNQVEQ VFAERDILS FADNPFVVS 
      MYCSFETRK HLCLVMEYV EGGDCASLL KNIGPLPSD 
      MARFYFAET VLAVEYLHS YGIVHRDLK PDNLLITAL 
      GHIKLTDFG LSKMGLMSL ATNLYEGYV DTEARQFSD 
      KQVFGTPEY IAPEVILRQ GYGKPVDWW SMGIILYEF 
      LVGCVPFFG DTPEELFAH TVQDDIEWP DSEDWPVQE 
      EAKDLITAL LQHSPRDRL GTGGAHEVK EHVYFSGLD 
      WNSLLRQKA EFVPQLEHD EDTSYFDSR IDRYNHELE 
      DDTDDTDDS PVFGLFSSC SPQYRKANV SKLSSSDPD 
      NSSDGIKIN TTVTTPDTP DVPDFKLRP RAPISLQTP 
      ERERQEIAP IEHRKISLD FGKTISTPE SSQTDSDDV 
      SPQIQRRRK TGHSRDILP KFSISVEDE HSSLETGTS 
      SSENRELSP LGRVQLSNK HKSRSVVKS ASTSGLSLM 
      IPSDEFQAP THNIHSPSG GGGSSTASS RDASPCREL 
      SPLVTSLKP PIIIRRGPK GFGFTVHTI RVYYGDTDV 
      YTMHHLVMA VDEGSPAFE AGLRPADLI THINGETVQ 
      GLYHTQVLQ LLLNGSEHV SLRATPLEQ TSIKTGGRK 
      REPGQSKLA KRSLHRQRK QKRDSDKRR KTSLFRKIS 
      NKRASVEMQ QIAAIQSPI SVTPSRSFQ SFPRSQDSS 
      FSSGPIRTP PTVNRSNLT LSDSSSSSQ TPSTSSPSA 
      TPATPRTKQ HYQRPSTLH GLKHKLHSS ACPKALHAA 
      SPSCTTAVP NRRKSVGHI PLSPLARTP SPSPLPASP 
      TRSPSPLAF PVGHQPGSS NTTQSYSPS AGTAPIGCN 
      QSKKGFVRP KGAEPGSPL LRRALSPDR LHPRSAESK 
      CSISPLCSS GNSPPVKAQ TRGSTVWRP NPQPEKDKE 
      AETEVANAQ TDDSRLSLN LSGPGELLP RIAEEKDSP 
      TNNGQEEKK CDKTSKKCD KEGCNNKTS DEKRERKCD 
      KGGSKPEMK KSDKAQSST SGVKAKDKS VQ
    CDS sequence
    • >TC004792 RA
      atggaggac aacaatgga gctaaatcg cgggcacgt 
      tcaaactcc gccagggtt ctgctcttc gacgacgtc 
      ggactgccc cacgacagg accaagccc ccgccgata 
      gcccccggg ccgcccccc aagcatgca cagccagat 
      catcagtcg acaccttcg aatttggtc cgaataagt 
      tcagctctg ggcaaatcg gctccgtcg ctgtcggtg 
      aatatgaag gatgtgaat gtggggcgc cgaaacagc 
      cgtcctgct gcagttcac cgcttgagc tttgtagca 
      aatacgtct cctgttcta ccgcgatct cagtcaccg 
      atatgtggc agtccgatt gatagtccc cgcacgcat 
      aatagtttc atcaatttt cccttcgcg ccgattaag 
      agaatcgtt gctgggtgt cggggggat gggaggcgg 
      tggtcagtt gcgtcgtta ccatcctcg gggtacgga 
      actacgcct ggtagttcc aacatgtcg tcgaagtgt 
      tcgagccag gaaagactg catcaacta ccacatgta 
      cctacgtcc gatgatatg aggattttg actcatcat 
      ttctcgagt aacgaaagt aatccttcg ttaccaacg 
      gggaattat gaggagaat cctataagt catcggtcg 
      cctttacac cgaccaaga tcgagaagt ctcagtagc 
      cctagcaga tctcctgtc atggacaac gaaatttgt 
      atgatgaac gttttgtac aaagagcgg ttcccgaaa 
      gccacgcaa caaatggag gagcgtctg aaccactac 
      atcgaagaa aataaaacg tttgacttc ggcgaagat 
      tgcgactat ctccctata gtgcgattt gttcaccat 
      caagtcttg gaaatggct cgcgattgt ttacacaaa 
      tcacagtcc aaattgatc acaagtcgg tatttctac 
      gaaatgtcg gaaaattta gaaagacta ttaactgaa 
      actaaagat aagtctgaa gaagctgct aatctagtc 
      accggtttt ataaaaaag ttgctgctg ataatatcg 
      cgtccggcc cgtctatta gaatgttta gagttcgat 
      cccgaggag ttctaccac tttttggag gccgcggaa 
      ggccaagtt aaggtggtg caaggcatc aaagccgac 
      atacctcag tacattatc caaaagctc ggcctaaac 
      cgagaccct attgccgag ctccaggaa gaacttcgc 
      gattgcaat tggtcgtcg aaccaatcc tcgcgttcg 
      tccgtttgc ctgacttca accccaagt acaaaaaag 
      tccctctct aatcccaac gaacaggat ttcgaagtc 
      gttaaatta atctcaaac ggtgcgtac ggcgctgtt 
      tacttggtc aaacacaag cagacgcgt caaaggttc 
      gctatgaag aaaattaac aaaaataac ttaatactg 
      cggaaccaa gtcgagcag gtgttcgcc gaacgtgat 
      atactcagt tttgcggat aatccgttc gttgtcagt 
      atgtactgt agctttgag acacggaag catctgtgt 
      ttggttatg gagtatgtg gagggtggc gattgcgct 
      agtttactt aaaaacatc ggaccactg ccgtcggat 
      atggcgagg ttttacttc gcggaaact gtcctcgct 
      gttgaatac ttacacagt tatggcatc gtacacagg 
      gatttgaaa ccggacaat ttgttaatc accgcgctc 
      ggacacatt aaattgacc gatttcggg ttaagtaaa 
      atgggactg atgtccttg gctacgaat ttatacgaa 
      gggtatgtc gatactgag gctcggcaa ttctccgac 
      aagcaggtt tttggtact cctgaatac atcgcacct 
      gaagtgata ctgagacaa gggtatggt aaaccggtc 
      gattggtgg tcgatgggt atcatcctc tacgagttc 
      ttggtaggt tgtgtcccc ttttttggc gacacgcct 
      gaagaactt ttcgctcat actgttcaa gatgatatt 
      gagtggccg gatagtgag gattggccg gttcaggaa 
      gaagcaaaa gatctcatt acagcgctg ttgcaacat 
      tcccctcgt gataggtta gggactggg ggcgctcac 
      gaagtcaaa gaacacgtc tatttcagt ggtctagac 
      tggaactcg ttgctgagg caaaaagcc gaattcgtt 
      ccgcagctg gaacacgac gaggacacc agctacttt 
      gacagccgc attgataga tacaaccac gaacttgag 
      gacgatacc gatgataca gacgactcg cctgtattc 
      ggcctattc tcctcttgt tcccctcaa tatcgcaaa 
      gcaaacgta tcgaagtta tcaagttcg gatcctgat 
      aactcctct gacgggatt aaaataaac acgactgtg 
      acaacgccg gatactccg gacgtgccc gacttcaaa 
      ttgcgacca agggctccg atcagtctc caaacaccg 
      gagcgcgaa cgccaggaa attgctcca attgaacac 
      cgcaagatt tctcttgat tttgggaaa acaatcagc 
      actcctgag tcgtctcag accgacagc gatgatgtt 
      tcgcctcaa atccagcga cggcgcaaa accggccat 
      tcgcgtgat attctacca aaattctca atatcagtc 
      gaagatgag cacagcagt ttagagaca gggacttcg 
      tcgtcggaa aatcgcgaa ttgtctcct ctggggcgc 
      gtccagtta tccaacaag cataaaagt cgctctgtt 
      gttaaaagt gcgtcgact tcagggtta tcacttatg 
      ataccatcg gacgaattt caagccccg actcataat 
      atacattca ccaagtggt ggcggtggt tctagtacc 
      gccagttcg cgtgatgct tcaccttgt cgcgagcta 
      tctcctctc gtcacgagc ttgaaacct ccgattata 
      atccgacga ggtccgaaa ggattcgga tttacggtt 
      catacgata cgtgtttat tacggcgat acggatgtc 
      tatactatg catcactta gttatggca gttgatgag 
      ggaagtccg gccttcgaa gccggttta agaccagcc 
      gacttaatt actcacatt aatggcgaa actgtacaa 
      ggactgtat cacactcaa gtgttacag ttacttttg 
      aacgggtct gaacatgtc agtttgagg gctacaccg 
      ttggagcaa acatcaatc aagacaggc ggccgcaaa 
      agggaacct ggacaaagc aaactggcc aagaggagc 
      ctccaccgg caaagaaaa caaaagagg gatagtgat 
      aaaaggcga aaaacgtca ctgtttcgc aagatcagc 
      aacaaaaga gccagcgtt gagatgcag cagattgca 
      gccatccag tccccgata agcgtgact ccgagccgc 
      agcttccag tcgtttccg cgaagccaa gacagttcg 
      ttttcttcg ggccccatc cgaacgccc ccaaccgtc 
      aaccgttca aacttgact ctctctgac tctagctca 
      tcgtcgcaa acgccctct acaagctca ccgagcgcg 
      actcctgcc acccctcga actaagcaa cactaccag 
      cgcccttca acccttcac ggcttgaaa cacaaactc 
      cactcaagc gcgtgccct aaggccctt cacgcagct 
      agtccttct tgcaccact gcagtcccc aaccggcgc 
      aaatcagtc ggacacata cccttgtcc cccttagcc 
      cgcaccccg tccccttcg cccctcccg gcctcccca 
      acgcgctcc cccagcccc ctggccttc cccgtgggg 
      caccagccg gggagctcc aacacgacc cagtcgtac 
      agccccagc gctgggacg gcacccatt gggtgcaat 
      cagtcgaaa aaagggttt gttcggcct aagggagcc 
      gagcctgga tcgcctcta cttcggaga gctctgtct 
      ccggatcgg ttacacccg agaagcgcc gaaagcaaa 
      tgctcgatt tcgccgctt tgctcatcg ggtaattcc 
      ccgccggtt aaagcgcaa acgcgggga agtacagtt 
      tggcgaccg aatccgcaa ccagagaaa gacaaggag 
      gcggaaact gaggttgcg aatgcccaa actgatgat 
      agtcggttg tctttgaat ttgtcggga ccaggcgaa 
      ttactgccg aggatagcc gaggagaaa gactcgccg 
      accaataac ggccaagag gagaaaaag tgtgataaa 
      acaagcaag aaatgtgat aaagaaggt tgcaataac 
      aaaacgagt gacgaaaag agagagagg aaatgtgat 
      aaagggggg agtaagccg gaaatgaaa aaaagtgat 
      aaggcgcag tcgagcacg agtggcgtt aaagcaaaa 
      gataaatcc gttcaataa
    mRNA sequence
    • >TC004792 RA
      ctcgattac acctacact gttagtgac gtcgcacaa 
      gccatcaat gaaagcaag tttagcgtt gcgcgcgca 
      cccgctgca gctgtttgt agtcttttg tcagattac 
      gtatccacg aaatatttt tatttatgt catacgaat 
      gggccaaaa attcgtaaa aacaagacg atggcccgt 
      attggtaga aattccccg actcgtgcc gttgtagaa 
      agaccgcgt tgtggccat gtgtgaacc aatgtttga 
      tccaagttc atgatattc atttttgga tccggttgt 
      gatagatgg aggacaaca atggagcta aatcgcggg 
      cacgttcaa actccgcca gggttctgc tcttcgacg 
      acgtcggac tgccccacg acaggacca agcccccgc 
      cgatagccc ccgggccgc cccccaagc atgcacagc 
      cagatcatc agtcgacac cttcgaatt tggtccgaa 
      taagttcag ctctgggca aatcggctc cgtcgctgt 
      cggtgaata tgaaggatg tgaatgtgg ggcgccgaa 
      acagccgtc ctgctgcag ttcaccgct tgagctttg 
      tagcaaata cgtctcctg ttctaccgc gatctcagt 
      caccgatat gtggcagtc cgattgata gtccccgca 
      cgcataata gtttcatca attttccct tcgcgccga 
      ttaagagaa tcgttgctg ggtgtcggg gggatggga 
      ggcggtggt cagttgcgt cgttaccat cctcggggt 
      acggaacta cgcctggta gttccaaca tgtcgtcga 
      agtgttcga gccaggaaa gactgcatc aactaccac 
      atgtaccta cgtccgatg atatgagga ttttgactc 
      atcatttct cgagtaacg aaagtaatc cttcgttac 
      caacgggga attatgagg agaatccta taagtcatc 
      ggtcgcctt tacaccgac caagatcga gaagtctca 
      gtagcccta gcagatctc ctgtcatgg acaacgaaa 
      tttgtatga tgaacgttt tgtacaaag agcggttcc 
      cgaaagcca cgcaacaaa tggaggagc gtctgaacc 
      actacatcg aagaaaata aaacgtttg acttcggcg 
      aagattgcg actatctcc ctatagtgc gatttgttc 
      accatcaag tcttggaaa tggctcgcg attgtttac 
      acaaatcac agtccaaat tgatcacaa gtcggtatt 
      tctacgaaa tgtcggaaa atttagaaa gactattaa 
      ctgaaacta aagataagt ctgaagaag ctgctaatc 
      tagtcaccg gttttataa aaaagttgc tgctgataa 
      tatcgcgtc cggcccgtc tattagaat gtttagagt 
      tcgatcccg aggagttct accactttt tggaggccg 
      cggaaggcc aagttaagg tggtgcaag gcatcaaag 
      ccgacatac ctcagtaca ttatccaaa agctcggcc 
      taaaccgag accctattg ccgagctcc aggaagaac 
      ttcgcgatt gcaattggt cgtcgaacc aatcctcgc 
      gttcgtccg tttgcctga cttcaaccc caagtacaa 
      aaaagtccc tctctaatc ccaacgaac aggatttcg 
      aagtcgtta aattaatct caaacggtg cgtacggcg 
      ctgtttact tggtcaaac acaagcaga cgcgtcaaa 
      ggttcgcta tgaagaaaa ttaacaaaa ataacttaa 
      tactgcgga accaagtcg agcaggtgt tcgccgaac 
      gtgatatac tcagttttg cggataatc cgttcgttg 
      tcagtatgt actgtagct ttgagacac ggaagcatc 
      tgtgtttgg ttatggagt atgtggagg gtggcgatt 
      gcgctagtt tacttaaaa acatcggac cactgccgt 
      cggatatgg cgaggtttt acttcgcgg aaactgtcc 
      tcgctgttg aatacttac acagttatg gcatcgtac 
      acagggatt tgaaaccgg acaatttgt taatcaccg 
      cgctcggac acattaaat tgaccgatt tcgggttaa 
      gtaaaatgg gactgatgt ccttggcta cgaatttat 
      acgaagggt atgtcgata ctgaggctc ggcaattct 
      ccgacaagc aggtttttg gtactcctg aatacatcg 
      cacctgaag tgatactga gacaagggt atggtaaac 
      cggtcgatt ggtggtcga tgggtatca tcctctacg 
      agttcttgg taggttgtg tcccctttt ttggcgaca 
      cgcctgaag aacttttcg ctcatactg ttcaagatg 
      atattgagt ggccggata gtgaggatt ggccggttc 
      aggaagaag caaaagatc tcattacag cgctgttgc 
      aacattccc ctcgtgata ggttaggga ctgggggcg 
      ctcacgaag tcaaagaac acgtctatt tcagtggtc 
      tagactgga actcgttgc tgaggcaaa aagccgaat 
      tcgttccgc agctggaac acgacgagg acaccagct 
      actttgaca gccgcattg atagataca accacgaac 
      ttgaggacg ataccgatg atacagacg actcgcctg 
      tattcggcc tattctcct cttgttccc ctcaatatc 
      gcaaagcaa acgtatcga agttatcaa gttcggatc 
      ctgataact cctctgacg ggattaaaa taaacacga 
      ctgtgacaa cgccggata ctccggacg tgcccgact 
      tcaaattgc gaccaaggg ctccgatca gtctccaaa 
      caccggagc gcgaacgcc aggaaattg ctccaattg 
      aacaccgca agatttctc ttgattttg ggaaaacaa 
      tcagcactc ctgagtcgt ctcagaccg acagcgatg 
      atgtttcgc ctcaaatcc agcgacggc gcaaaaccg 
      gccattcgc gtgatattc taccaaaat tctcaatat 
      cagtcgaag atgagcaca gcagtttag agacaggga 
      cttcgtcgt cggaaaatc gcgaattgt ctcctctgg 
      ggcgcgtcc agttatcca acaagcata aaagtcgct 
      ctgttgtta aaagtgcgt cgacttcag ggttatcac 
      ttatgatac catcggacg aatttcaag ccccgactc 
      ataatatac attcaccaa gtggtggcg gtggttcta 
      gtaccgcca gttcgcgtg atgcttcac cttgtcgcg 
      agctatctc ctctcgtca cgagcttga aacctccga 
      ttataatcc gacgaggtc cgaaaggat tcggattta 
      cggttcata cgatacgtg tttattacg gcgatacgg 
      atgtctata ctatgcatc acttagtta tggcagttg 
      atgagggaa gtccggcct tcgaagccg gtttaagac 
      cagccgact taattactc acattaatg gcgaaactg 
      tacaaggac tgtatcaca ctcaagtgt tacagttac 
      ttttgaacg ggtctgaac atgtcagtt tgagggcta 
      caccgttgg agcaaacat caatcaaga caggcggcc 
      gcaaaaggg aacctggac aaagcaaac tggccaaga 
      ggagcctcc accggcaaa gaaaacaaa agagggata 
      gtgataaaa ggcgaaaaa cgtcactgt ttcgcaaga 
      tcagcaaca aaagagcca gcgttgaga tgcagcaga 
      ttgcagcca tccagtccc cgataagcg tgactccga 
      gccgcagct tccagtcgt ttccgcgaa gccaagaca 
      gttcgtttt cttcgggcc ccatccgaa cgcccccaa 
      ccgtcaacc gttcaaact tgactctct ctgactcta 
      gctcatcgt cgcaaacgc cctctacaa gctcaccga 
      gcgcgactc ctgccaccc ctcgaacta agcaacact 
      accagcgcc cttcaaccc ttcacggct tgaaacaca 
      aactccact caagcgcgt gccctaagg cccttcacg 
      cagctagtc cttcttgca ccactgcag tccccaacc 
      ggcgcaaat cagtcggac acataccct tgtccccct 
      tagcccgca ccccgtccc cttcgcccc tcccggcct 
      ccccaacgc gctccccca gccccctgg ccttccccg 
      tggggcacc agccgggga gctccaaca cgacccagt 
      cgtacagcc ccagcgctg ggacggcac ccattgggt 
      gcaatcagt cgaaaaaag ggtttgttc ggcctaagg 
      gagccgagc ctggatcgc ctctacttc ggagagctc 
      tgtctccgg atcggttac acccgagaa gcgccgaaa 
      gcaaatgct cgatttcgc cgctttgct catcgggta 
      attccccgc cggttaaag cgcaaacgc ggggaagta 
      cagtttggc gaccgaatc cgcaaccag agaaagaca 
      aggaggcgg aaactgagg ttgcgaatg cccaaactg 
      atgatagtc ggttgtctt tgaatttgt cgggaccag 
      gcgaattac tgccgagga tagccgagg agaaagact 
      cgccgacca ataacggcc aagaggaga aaaagtgtg 
      ataaaacaa gcaagaaat gtgataaag aaggttgca 
      ataacaaaa cgagtgacg aaaagagag agaggaaat 
      gtgataaag gggggagta agccggaaa tgaaaaaaa 
      gtgataagg cgcagtcga gcacgagtg gcgttaaag 
      caaaagata aatccgttc aataattct agcttagta 
      ttacgcaac ttgccaaac attttgtat tatatttgc 
      ttattttaa atcgctcag aaatctcgt ttagttgct 
      ttcatacta aaaaagaca acacatagg tagtatgtg 
      tcttaaaaa tatactcat atgttaggg tgagaccag 
      gtgtgattt tgtttcgtg tgcatgtaa ctcatttcg 
      attaaatct tgtatattt gtgtaacag ttgtgtaac 
      ttcattcat gagtggatt atttgcttg ggtatactt 
      tcaagagac atctacgac tggtgaagg ttttaattt 
      gcttcgttt aatacattc aaaattcgg aaatgtcag 
      ccattttac cgcagtatg tgtcaattg aattttttt 
      aactacgtg tttaataag tgtatgcac tggctatac 
      aatatatat tcttagaat aattaacat gctagatgt 
      tagttttaa ttgggatct caattactt ttgcaatgt 
      gttagccat attttgttc tacaaattg gccaaattt 
      tctttagcc acactcaac gcaactttg tgtaaattt 
      ctccaagta tattaatta cattactgt atttttcaa 
      acgccaaat agtttttta ttcgttatg ctgcgacca 
      gtagttcaa atcaaatat ttttgatta gtatttctg 
      ttcaagcgg gaagtttga aaatcccaa ttgacgtaa 
      gttggcaac tgaagaggc gttgtgcgt ggctgggtc 
      gaatttcgc ccacaaatt agtctagga tacctggta 
      attagcaaa ccaaacgaa tttgcatat attctgttg 
      tacttaaat cgaaaagaa tatttatta ctgaaagtt 
      ttcatgcgt tatactaca aagttgaaa taagttgtt 
      aggatgtta tatagaagt aatatattg tatattgat 
      tttttaatc tgaaattaa acggcgcct tttacgttc 
      attttaatt agtgt