General information | |||
TC004789 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 7235464..7240289 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004789 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03846 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
Comment: No phenotpe found! |
- >TC004789 RA
MANQYHKLE AKAVQELKD IANKIRIHS VSSTQTAKS GHPTSCSSI AEILSVLFF NTMRYKISA PKDPSSDRF VLSKGHAAP ALYAVWAEA GLFPVADLQ NLRKLGSDL EGHPTPRLN FIDVGTGSL GQGLAIACG MAYVGKNFD KASYRVYCL VGDGESAEG SIWESLHFA TYYKLDNLC VIFDVNRLG QSEPTSLGH DIDTYRKRL EAFGFNVVA VDGHDVEEL VKAFHVSCN TKGKPTAII AKTFKGKNF PKIEDLENW HGKPLGDQA DRVLQHLKS LVKNTGPLQ LHPQKPLTD DAPIVDISN IKLSSPPSY KIGDSIATR VAYGTALVK LGQSNPRVI ALDGDMKNS TYSEALKKF DPTRYIECF IAEQNLVGV AIGATCRDR TVAFVSTFA TFLTRAYDQ IRMGAISQT NVNFCGSHC GVSIGEDGP SQMGLEDIA MFRTIPGST VFYPADAVS TERAVELAA NTKGVTFIR TNRPATAVI YPNNHVFQV GKSHVVKKS NNDQVLVIG GGVTLQEAI GAANELAKA GVGVRVLDL FTIKPIDKE GIIKNAKEC GGRILVVED HYYEGGIGE AVLSAVAQE RNIAVKHVA VPKVPRSGS PTALLDYYG LNSKHIVNY VQEAMKL
- >TC004789 RA
atggcaaat caataccac aaactcgaa gccaaggct gtccaagag ctgaaggac atcgccaac aaaatccgg atccattcg gtcagctcc acgcaaacg gcaaagtcg gggcatcca acatcatgc tcgtcaatc gccgaaatt ttatcggtc ctttttttc aacacaatg cgatacaaa atatcagca ccgaaagac ccctcaagt gaccgcttt gtcctttct aaaggtcac gccgctccg gcgctgtac gcagtatgg gccgaggcg ggtcttttc cccgttgct gatttacaa aacttgagg aaattgggg tcggacctc gagggtcat ccaactcct cgcttgaat ttcattgat gtagggaca ggctcgctg ggccaaggg ctggccatt gcgtgcggc atggcctac gtgggcaaa aacttcgac aaggccagt tatagggtg tactgcctg gtcggggat ggggagagc gccgagggt tcaatctgg gaatcgctc cattttgcg acttactac aagctcgat aatctctgc gttatcttt gatgtgaac cgtctcggg caatctgag cccacttct ctgggtcat gatatcgac acttacagg aaacggttg gaagcgttc ggtttcaat gtagttgcc gtcgatgga cacgacgtc gaagagctg gtgaaagcc ttccacgtg tcgtgcaac accaaaggg aagcctacc gcaatcatt gcgaaaact ttcaaaggg aagaacttc cccaaaatt gaagatttg gaaaactgg cacgggaaa ccactagga gatcaggcc gatcgtgtc ctccaacac ctcaaatct cttgttaaa aacacgggg cctttgcaa ctccacccc cagaagccc ctcaccgac gatgctccc attgttgac atcagcaat attaaacta tcctcgcct ccatcttac aaaatcggc gatagtatt gctacgaga gtggcctac ggcactgct ttggtcaag ctcggccaa agcaacccc cgagtcatt gcccttgac ggcgatatg aaaaattca acatattcg gaagccctt aaaaaattt gaccccacg agatacatc gaatgtttc atcgccgaa cagaacctt gtcggggtt gccattggt gctacttgt cgcgaccgc acagttgcc ttcgtttcc acctttgct actttctta acccgtgct tatgaccag attcgtatg ggggctatt tctcagaca aatgtcaat ttctgcggc tctcattgt ggtgtcagt ataggcgaa gacggacca agccagatg gggctggaa gacatcgct atgttccgg accatcccc ggaagcact gtcttctac cccgctgat gcggtctcc actgaacgt gccgtcgaa ctggccgcg aacaccaaa ggagtcact ttcatcaga accaacaga ccggccact gctgtcatc taccccaac aatcacgta ttccaggtg ggcaagtcg cacgtggtg aagaagtcg aacaacgac caagtcctg gttattggt ggcggagtg accctgcag gaggccatc ggggccgcc aacgaattg gctaaagcc ggtgttggt gtccgtgtt ttggatctg ttcaccatc aagccaatt gacaaggag ggaataatc aaaaacgcg aaagaatgc ggtgggcgt attctcgtc gttgaagac cactactac gaggggggc attggcgag gctgtcctt tcggcggtc gctcaggag cgcaacatc gccgtgaaa cacgtggca gtgcccaaa gtgccccgc tccgggagc cccaccgcc cttttggac tactacggc ctcaacagc aaacacatt gtcaattac gtgcaagaa gcaatgaag ttgtaa
- >TC004789 RA
atcattcaa gtatcaact tatcgactt atcgtcaat aattcagat ttgaaatat cgctaaata aaaacagat tcgactcat gaataatta cacacaaca tgtattttg caatggcct tgcaaatga taatgaaga gttattatt gttacataa ctggaaact tattttctt taattaatg caaaacatc aatcgcaaa atgcgccat tcttaattg ccggcaata aagttttgc acagatgta aataatttt atagcgttg ctatgggta gggaccctt taaatatcg tgagtcagc aatacggaa cctcatttt tcaaggata ggctctcat tgcagtaat tgcacttct attattgtc cctcgtgtc atcctgcgc attaaacaa tgcaagatg caccgtgtt tgtaaataa ataaaattc attcgagaa aaatacaaa gtcaaaatt ttgacccga ttatatcat ttttgatta tttataaac tctctgcag gggtgccgg ggttacttg atagcacag ttgtcgata gtttgggaa atttgggat gatttggcg ttattatat ctcagcttg cgttttttt cgattttta tttataaat tctctgctg gggttactc gataactta agcgttgat aatattgat cgttatgga aaataaaat catgtggaa aatgtgtga tttgatggt cacacaact gctgttacg gctgattcg agtcatttg attcaattt ttggcgcca aaactggcc catgtcttg cggtcattt cagtttgat tgaaaaata tgtgttttt gactattgg aaaattgtg tgcgcatgc ttccaggtg aaacgcgat tgccttgac tccgggcac ctaaaaaat gtctcagag aaagtcccg agtcgaaaa attggtgta ttttattta gaaatgtct tagcgttac gtaattgtt atctgaccc agcttccaa tagcgtcaa tgtacgtaa catagcttg atgttagcg aggtccgca ttggctgtc cctcaaggt gtgtgcacg cctgccatt taaccactt gttgattac atgctggtg ccaagtgaa cgggttagc tctagctcc tgaccggtc tttccctcg atgtccgct gatataatg gcaaatcaa taccacaaa ctcgaagcc aaggctgtc caagagctg aaggacatc gccaacaaa atccggatc cattcggtc agctccacg caaacggca aagtcgggg catccaaca tcatgctcg tcaatcgcc gaaatttta tcggtcctt tttttcaac acaatgcga tacaaaata tcagcaccg aaagacccc tcaagtgac cgctttgtc ctttctaaa ggtcacgcc gctccggcg ctgtacgca gtatgggcc gaggcgggt cttttcccc gttgctgat ttacaaaac ttgaggaaa ttggggtcg gacctcgag ggtcatcca actcctcgc ttgaatttc attgatgta gggacaggc tcgctgggc caagggctg gccattgcg tgcggcatg gcctacgtg ggcaaaaac ttcgacaag gccagttat agggtgtac tgcctggtc ggggatggg gagagcgcc gagggttca atctgggaa tcgctccat tttgcgact tactacaag ctcgataat ctctgcgtt atctttgat gtgaaccgt ctcgggcaa tctgagccc acttctctg ggtcatgat atcgacact tacaggaaa cggttggaa gcgttcggt ttcaatgta gttgccgtc gatggacac gacgtcgaa gagctggtg aaagccttc cacgtgtcg tgcaacacc aaagggaag cctaccgca atcattgcg aaaactttc aaagggaag aacttcccc aaaattgaa gatttggaa aactggcac gggaaacca ctaggagat caggccgat cgtgtcctc caacacctc aaatctctt gttaaaaac acggggcct ttgcaactc cacccccag aagcccctc accgacgat gctcccatt gttgacatc agcaatatt aaactatcc tcgcctcca tcttacaaa atcggcgat agtattgct acgagagtg gcctacggc actgctttg gtcaagctc ggccaaagc aacccccga gtcattgcc cttgacggc gatatgaaa aattcaaca tattcggaa gcccttaaa aaatttgac cccacgaga tacatcgaa tgtttcatc gccgaacag aaccttgtc ggggttgcc attggtgct acttgtcgc gaccgcaca gttgccttc gtttccacc tttgctact ttcttaacc cgtgcttat gaccagatt cgtatgggg gctatttct cagacaaat gtcaatttc tgcggctct cattgtggt gtcagtata ggcgaagac ggaccaagc cagatgggg ctggaagac atcgctatg ttccggacc atccccgga agcactgtc ttctacccc gctgatgcg gtctccact gaacgtgcc gtcgaactg gccgcgaac accaaagga gtcactttc atcagaacc aacagaccg gccactgct gtcatctac cccaacaat cacgtattc caggtgggc aagtcgcac gtggtgaag aagtcgaac aacgaccaa gtcctggtt attggtggc ggagtgacc ctgcaggag gccatcggg gccgccaac gaattggct aaagccggt gttggtgtc cgtgttttg gatctgttc accatcaag ccaattgac aaggaggga ataatcaaa aacgcgaaa gaatgcggt gggcgtatt ctcgtcgtt gaagaccac tactacgag gggggcatt ggcgaggct gtcctttcg gcggtcgct caggagcgc aacatcgcc gtgaaacac gtggcagtg cccaaagtg ccccgctcc gggagcccc accgccctt ttggactac tacggcctc aacagcaaa cacattgtc aattacgtg caagaagca atgaagttg taattttat catgtttac taatggagc ggtgtaaca ttccatttt aataaaaaa attctaagt ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
