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Gene information
General information
TC004778 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6644562..6653033
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004778
    iBeetle screen iB_03845
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Pupal morphology
    maxillary palp size elongated - Number of animals showing the phenotype: 5

    antenna orientation irregular - Number of animals showing the phenotype: 9

    elytra partially swollen - Number of animals showing the phenotype: 2

    elytra dorsal orientation irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004778 RA
      MNVKQERHD EAEIQALAD KIKEASKNR MAVNQQKAH 
      VGLGQNLIL SSMMNDKKK QNNTLQATN CQETGKSID 
      EDSVRGRFG WETIGKNPI PYINRNGEK YCAVRMVEM 
      KALNKYLNY LHQDIYNCT CIRSYYITE PEGRLLNEI 
      NMRHCDYHF GKEQFTPRD LIVRLSDAV EFYNFLCSC 
      YTKLVNGET DSLGRSGFI RINKESVVP YTVYNDQKY 
      VPLFYFEGE TDNLKLRAN KLEGWDLSY LKFCCKVQG 
      IRNELFSHD SCSVISLND IKNYFPPGT LFEDYWPKK 
      SVDSQLLIS GNTRNQSQI IQWTRQPAA PPVAHSIPS 
      PRAVSLTQP KPPAANSQM HTVHTYSSY GMAGQHPYH 
      PPQSRTPGA PIRTTYPAA AGNRHMRSP NYYSNITQG 
      PPPPLVPTT QAQPNLHYT NAQTNATPN YMLTGEQPN 
      LNQINYHQE EQDDSQPPP MIPIRQFLA NGGVLSPTF 
      LRQQQQARM HMDAMNQNT ATRDLQSNY NVSNRQSIL 
      INQQPAHQQ SSRTQPPTT FAEEQHSMS NGQMCLDDV 
      NCKLISIPE PPTGSNHIP YKMQKALVE GKLLPCINM 
      KPYVYSELL VTLPDLTVN FFNNIPVQS CQRVMKVLG 
      IDLYKPNNS QTHVLMESG KCQSINDVV PLVQVRNVI 
      DFMPQLKYM LGGVLPNIM SNKRQRNS
    CDS sequence
    • >TC004778 RA
      atgaacgtg aagcaggaa cggcacgac gaggccgaa 
      attcaggcc ctcgccgac aagatcaaa gaagcgagc 
      aaaaatcgg atggcagtg aaccagcag aaggcccat 
      gtgggcctg ggccagaac ctcatcctc tcctccatg 
      atgaacgac aagaagaag caaaacaac accctccag 
      gccaccaac tgccaggag accgggaag agcatcgac 
      gaggactcc gtgcggggg cggttcggc tgggagacc 
      atcggcaag aaccccatc ccctacatc aaccgcaac 
      ggcgagaag tactgcgct gtgcggatg gtggagatg 
      aaggccctc aacaaatac ctgaactac ttgcaccag 
      gacatctac aactgcacc tgcattagg agctactac 
      atcacggag cccgagggc cggctcctc aacgaaatc 
      aacatgcga cactgtgac taccacttc ggtaaagaa 
      cagttcacc cctcgggat ctgatcgta agactgagt 
      gatgctgtg gagttctac aacttcctc tgttcgtgc 
      tacacgaag ttagtaaac ggtgaaacg gattcttta 
      ggacgcagc ggcttcatc cgaattaac aaagaatca 
      gtggtgccc tacacggtg tataacgac caaaaatac 
      gtccccttg ttctacttc gagggcgag acggacaac 
      ctcaagcta cgagccaac aaactcgaa ggctgggac 
      ttatcctat ttaaaattc tgttgtaaa gtacaagga 
      attcggaac gaacttttc tcgcacgat tcctgttcg 
      gtgattagt ttaaacgat attaaaaat tatttcccc 
      ccggggacc ctgttcgag gactactgg ccgaagaaa 
      agtgtcgat tcccagttg ttaataagc gggaatacg 
      cggaaccaa tcgcagatc atccagtgg acgcgtcag 
      ccggcagcc cccccagtt gcccattcg atccccagt 
      cctagggct gtcagtttg acgcagccg aagcccccg 
      gccgccaac agccagatg cacacagtg cacacctac 
      tcctcctac ggcatggcg ggacagcat ccctatcat 
      cccccgcaa tcccgcact cccggagcc cccatcagg 
      accacctat cccgcggca gcagggaat aggcatatg 
      aggtcgcct aattactac tcgaacatt actcaggga 
      ccaccgccg ccgcttgta cccacaaca caggcgcaa 
      ccgaactta cactatacg aacgcccag acgaacgca 
      acgccgaat tatatgtta accggagaa cagcccaac 
      cttaaccaa atcaattac caccaggaa gagcaggac 
      gactcgcaa ccacctcca atgattccc attaggcaa 
      tttctggcg aatggtggt gtgctttcg cccacattc 
      ttaaggcaa cagcaacaa gcgaggatg catatggac 
      gccatgaac caaaatacg gccacacgg gacttgcaa 
      agtaactac aacgtcagc aaccggcag tcaattctg 
      atcaaccag caaccagcc catcaacag tcatctcgg 
      acccagcct cccacgacc ttcgccgag gagcaacac 
      tcgatgtcc aacggccaa atgtgctta gacgacgtc 
      aactgcaag ctaatatca attcccgaa cctccgact 
      ggcagcaac cacattcca tataaaatg caaaaggcg 
      ctagttgaa gggaaacta ctaccgtgt attaatatg 
      aagccatat gtttactcc gaacttctg gtgactcta 
      cctgatctc acagtgaac ttttttaac aatatacca 
      gtgcaatca tgtcaaaga gtcatgaaa gtgctaggc 
      atcgacctg tacaaaccc aacaattcc caaacccac 
      gtgctgatg gagtctggc aagtgccag agcataaac 
      gacgtggta cctttagtt caagtgcga aatgtgatc 
      gacttcatg cctcagctg aagtacatg ttggggggc 
      gttttacct aacataatg tcaaataag cggcagagg 
      aacagctag
    mRNA sequence
    • >TC004778 RA
      cccgcaaga tgtcagtgc gtttttagt acgttattt 
      cgccgtgag ttgtcgtta ggttagtta tttttgatg 
      ccatctagc tgtaccccg ctttcgaac gtttcgtgt 
      actaatatg agtgcagtg gtgttaagt gtgtgtgaa 
      gaaaaccct ggcttccaa tgtttttaa actaaagtt 
      aaattgcgt tagacgggg tgaaaaatc tggtacagt 
      gtgttgtgg cgaatttgg gttaggtcc cgtagtttt 
      cgcatgaat tcggttcgg caagagagt ttaacaccg 
      agtgttcca ttttgatgg cgctatggc cgctaaaac 
      ataaccagg gaaaataca aacccgcct taacctcac 
      tcttggtaa acatcaatc gaacgaaaa tgggttgca 
      cgatctagt cttttgttt ggagagttg tcaacttgt 
      caaaatgaa cgtgaagca ggaacggca cgacgaggc 
      cgaaattca ggccctcgc cgacaagat caaagaagc 
      gagcaaaaa tcggatggc agtgaacca gcagaaggc 
      ccatgtggg cctgggcca gaacctcat cctctcctc 
      catgatgaa cgacaagaa gaagcaaaa caacaccct 
      ccaggccac caactgcca ggagaccgg gaagagcat 
      cgacgagga ctccgtgcg ggggcggtt cggctggga 
      gaccatcgg caagaaccc catccccta catcaaccg 
      caacggcga gaagtactg cgctgtgcg gatggtgga 
      gatgaaggc cctcaacaa atacctgaa ctacttgca 
      ccaggacat ctacaactg cacctgcat taggagcta 
      ctacatcac ggagcccga gggccggct cctcaacga 
      aatcaacat gcgacactg tgactacca cttcggtaa 
      agaacagtt cacccctcg ggatctgat cgtaagact 
      gagtgatgc tgtggagtt ctacaactt cctctgttc 
      gtgctacac gaagttagt aaacggtga aacggattc 
      tttaggacg cagcggctt catccgaat taacaaaga 
      atcagtggt gccctacac ggtgtataa cgaccaaaa 
      atacgtccc cttgttcta cttcgaggg cgagacgga 
      caacctcaa gctacgagc caacaaact cgaaggctg 
      ggacttatc ctatttaaa attctgttg taaagtaca 
      aggaattcg gaacgaact tttctcgca cgattcctg 
      ttcggtgat tagtttaaa cgatattaa aaattattt 
      ccccccggg gaccctgtt cgaggacta ctggccgaa 
      gaaaagtgt cgattccca gttgttaat aagcgggaa 
      tacgcggaa ccaatcgca gatcatcca gtggacgcg 
      tcagccggc agccccccc agttgccca ttcgatccc 
      cagtcctag ggctgtcag tttgacgca gccgaagcc 
      cccggccgc caacagcca gatgcacac agtgcacac 
      ctactcctc ctacggcat ggcgggaca gcatcccta 
      tcatccccc gcaatcccg cactcccgg agcccccat 
      caggaccac ctatcccgc ggcagcagg gaataggca 
      tatgaggtc gcctaatta ctactcgaa cattactca 
      gggaccacc gccgccgct tgtacccac aacacaggc 
      gcaaccgaa cttacacta tacgaacgc ccagacgaa 
      cgcaacgcc gaattatat gttaaccgg agaacagcc 
      caaccttaa ccaaatcaa ttaccacca ggaagagca 
      ggacgactc gcaaccacc tccaatgat tcccattag 
      gcaatttct ggcgaatgg tggtgtgct ttcgcccac 
      attcttaag gcaacagca acaagcgag gatgcatat 
      ggacgccat gaaccaaaa tacggccac acgggactt 
      gcaaagtaa ctacaacgt cagcaaccg gcagtcaat 
      tctgatcaa ccagcaacc agcccatca acagtcatc 
      tcggaccca gcctcccac gaccttcgc cgaggagca 
      acactcgat gtccaacgg ccaaatgtg cttagacga 
      cgtcaactg caagctaat atcaattcc cgaacctcc 
      gactggcag caaccacat tccatataa aatgcaaaa 
      ggcgctagt tgaagggaa actactacc gtgtattaa 
      tatgaagcc atatgttta ctccgaact tctggtgac 
      tctacctga tctcacagt gaacttttt taacaatat 
      accagtgca atcatgtca aagagtcat gaaagtgct 
      aggcatcga cctgtacaa acccaacaa ttcccaaac 
      ccacgtgct gatggagtc tggcaagtg ccagagcat 
      aaacgacgt ggtaccttt agttcaagt gcgaaatgt 
      gatcgactt catgcctca gctgaagta catgttggg 
      gggcgtttt acctaacat aatgtcaaa taagcggca 
      gaggaacag ctaggactg ggcgaacct gtggggcta 
      taactttta caatctgtg aaagtccac gaactgaag 
      tgttttgta aaagtaaca aatgtgttg ttttatata 
      aaaattgtt cctaaagtg taaattaat aagtactat 
      gcagaaaag tgaaactac tatgtacta tgcgctagt 
      tacacaaat gatttttat ttttgtcat tagtgcgtg 
      cttttagaa cgtcgatgc atgccggac tcgttcagt 
      acaaccgtt ttgttgtta atttgacca ttttttttg 
      gaatgtttt gtgttttat cttcgtatt tttgttatg 
      aactcttgt ttagttgag gtatggtcg acgttttaa 
      accgcgtgt tgaacgttt cgatcctgt tggcattag 
      aataggtat tttgaattg tttgatcac tttttgctc 
      attcaaaaa gttttcttt ggttattga gaatttttt 
      aatttggcg tctttctag tcaattaaa ttaagcagc 
      gtttaaaat attccaaca aaagtggta attatttga 
      aatatctgc tctattttt cgtatataa ttatttatt 
      tcttcaatg atcattata tataataga tagttatat 
      caatgtgaa atgtccagg gtaggggat gtactttag 
      ataaacatt ctggattta gataataac attgtaaca 
      cttttttga gatctgaca tttttcaag tggtgtttc 
      gtaaacgat tttgtgttg atttccttg gaaacatgt 
      ttattccct tgcctgtga tatttgaac agattacaa 
      atcagtatt taataaatt tattatgca ttgagcttc 
      aacactcaa ttaaattcc ttattgcta cttaacatt 
      ttggctctc tggcaaaat caattatgt atctgtaca 
      aaatgtttg tagaattac ttttctatg taatttttt 
      agcaattgt ttctagtag tgttgttat actttggca 
      tttttttca cacagatgt agttgtaat gttactatg 
      tatatgtat atagtaaac atttattca aataaatct 
      tctaagttc tattattaa tttctggtt tgactatta 
      ctagaaatt ttgttgcca aatatactt caaacgaaa 
      ctccatcac ttacaaacc tgaaccgga tttctttac 
      ttgtattat gataatttt gtagcgtag gaaccagta 
      gcatttttt taatataaa ttttatctg ttacttcgt 
      ttaccatgt aaataaaat attttcata tgatttttt 
      tttctaaat catatttca acttcgcct gctatagga 
      tgtgtttct gcaactaat aacccaggc ttacgtttt 
      ttccgtagt ttaaccgtc caaactacc gaatttgca 
      atttagtta ttatttatt ttgtacttg tttcatgtt 
      cattagttc attgttttt gttccctga gtttcatta 
      gaataaatt atttattct tta