General information | |||
TC004777 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6859245..6867779 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004777 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03844 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
leg musculature not present - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: body wall muscle pattern ok. coxa musculature present. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
elytra sometimes surface irregular - Number of animals showing the phenotype: 6
elytra dorsal orientation not closed - Number of animals showing the phenotype: 6
- >TC004777 RA
MVFPKTTPL QNQSRAILK LLDKNLVNR EYVEYQFIQ QSQIPTSHF QSALPRLPI PELKLTCER YLAAQRPLL IDESYKKTE ANVYRFRDG VGKQLQKIL KDYDNKNKH TSYISELWF DKYLRSRSP LPLNCNPVL VLNNDDKPE YNNQLIRAT NLIVSSLRF YRALKSELL HPEIFHLNP KKSDTERLN FVLSKLPPK LAYICAYFN KAFPLDMSQ YPSLFQTTR IPETDKDRL VSCPESKHI TVQYKGRFY SFEVLTVSN DIVSPTQIM DNIKFILSD KTDSDKFPI GVLTTLERN KWATIRHEL AENGNEALL KTIDSALFH VCLDEETVG SDPYKITKQ FLHADGANR WFDNSFSLI VARDGAAAV NFEHAWGDG VAVLRYAQD IYNDAKNKP RVHPDTALT EDSSCQVQR LEVHLNDHL RKCVDDAKN SYKTICDNL DVNYTIFEG IGKDTCKKS QVSPDAIMQ LAFQLCCEK QSPIDDLNS SIRRSSFGW SIVSAIIMD AYEVELYGD YPNLNNNEL PYIIGCLHT KNMEAVQKF FLRGLYKDV KIHQITRWK RNCQPVCVY TVVYKIKVR TPDLSEEEI FIPFPDNEE DLKKMFRHI TEMVFGMRV VEKKLSDDG VFASISYAP VRCACNVRN FDMAAEEPR MQPLITEVA YHKINGLFV STYESCSTS AFKHGRTEA IRPCTTATK DVTLAINAL NKPPVAQLK KMIQECSTI HNRLIKEAA MGQGFDRHL FALKSMGEK NDIKSNIFD DPEYSHMGY DILSTSTLN SPAIFAGGF GPVVPEGFG LGYVIKDEF LGTLATNYK DRTNGPDFM GALTKSFEE IFAILRTN
- >TC004777 RA
atggtcttc cccaaaaca acacccctt cagaaccaa tcacgggcg attttaaag ctccttgac aagaactta gtcaatcgg gaatacgtg gaataccag ttcattcaa cagagccaa attccaacc tcacatttc caatcagca ttgccccga cttccgatt cctgaatta aaattaacg tgtgagcgg tacttggcg gctcagcgg cctttgtta atcgacgag tcgtacaaa aaaaccgaa gccaacgtc tatcgcttc cgggacggg gttggcaaa cagttacaa aaaatttta aaagactac gacaataag aacaagcac acgagttac ataagtgag ttgtggttc gacaagtat ctaagatcg cgaagtcct ttaccgcta aactgcaac ccggtcctg gttttaaac aacgacgat aaaccggaa tacaacaat cagctcatt agagccaca aatttgatt gtcagctct ttgcgattt taccgtgcc ttaaagtcg gagttgttg caccccgaa attttccac ttgaatccg aaaaaaagc gacacagag cggctgaac ttcgtcttg agtaaattg ccgccgaaa ttggcctac atttgtgcg tattttaac aaggcgttc cctttggac atgagtcaa tatccgagt ttgttccaa acgactcgt attcctgag actgataag gataggctt gttagttgt cccgagtct aaacacatt acagtgcag tacaaaggt cgcttctat tctttcgaa gtattaact gttagtaat gatatcgtc tcgcccacg caaatcatg gacaacatc aagttcatc ctgagtgat aagacagat tctgataag tttccaatt ggggtcctc acgaccctc gagcgcaac aaatgggcc acaattcgc cacgaactg gccgaaaac ggcaacgag gccctgctt aaaacaatc gattcggcc ctgtttcac gtctgtctg gacgaagaa acggtcgga agcgaccct tataaaata actaaacag tttttgcac gccgatggg gccaataga tggttcgat aattcgttt tcgctgatt gtggcgaga gatggcgcc gctgcggtc aatttcgag cacgcgtgg ggagatggc gttgctgtc ttacgttac gcacaagac atttacaat gatgcaaaa aataagcca agggtgcat cctgatacc gctctaacg gaggattct tcctgccaa gttcagcgt ctagaagtt catctgaat gatcatttg agaaaatgt gtcgatgat gcgaaaaat tcgtataaa acgatttgt gataatctt gatgtcaat tatacgatt tttgagggg attggaaag gatacgtgc aagaagagt caagtcagt ccagacgcg atcatgcaa ctggcgttc cagctctgc tgtgaaaaa caatcgccc atcgacgat ttgaactcg tcgattcgt cgcagtagt tttggttgg agtattgtg tccgccatt ataatggat gcgtatgaa gtggaatta tatggagat tatccgaat ttgaataat aacgaattg ccgtatata attggttgt ctacataca aaaaacatg gaggccgtc cagaagttt tttttgcgg ggcttatac aaagatgtg aagatacat caaatcact cgatggaaa agaaattgc cagccagta tgtgtgtat acggtggtt tacaaaatc aaggttagg actcccgat ttaagtgaa gaggaaatc ttcattcca tttccagat aatgaagaa gatttaaaa aaaatgttc cgtcatatt actgaaatg gtttttgga atgcgtgtg gtcgaaaaa aaactcagt gatgatgga gtcttcgca tcaataagc tatgccccg gttcgttgt gcatgcaat gtgcgaaat tttgatatg gctgcagag gagcctcgc atgcagccc ctaattaca gaggtggct tatcacaaa ataaatggg ctttttgtg tccacctac gagtcgtgc agcacttct gctttcaag cacggcagg acggaagca attcgtccg tgcacgacc gccaccaag gacgttaca ttggctatt aacgcgttg aataagcca ccggtggct caactgaaa aaaatgatt caggagtgt tcgacgatc cacaatagg ctgatcaag gaagctgcg atggggcaa ggatttgat cggcacctt ttcgccctt aaatcaatg ggcgaaaag aacgatatt aagagcaac atttttgac gatcctgag tattcgcac atgggttac gatatactc tcgacgagt actttgaac tcgccggcc atttttgcg gggggtttt ggaccagtg gtgccggaa gggttcgga ttggggtat gtgattaaa gacgagttt ttggggacg cttgcaacg aattataag gaccgtact aatggacca gacttcatg ggtgctttg acaaagtca ttcgaggaa atttttgca attttgcga accaattag
- >TC004777 RA
agagacaaa ctttttaat cattgtccc ttattaaag ccttgtctt gattccacc ctttactta tctgaattc gggggttgg tgcgagaaa tgacttgac agtctgtgt ttgacaatg acagttcag tttgacagt agaatgtgt aggtaaaat ttgttttgt tgaaatttt cctcatatt tcttcaaaa tttagttta atttaaatg tagacacgt gcccgtgct tcttttcgt aattagtta atttcgcag tgtgtcagt taatcaaaa atggtcttc cccaaaaca acacccctt cagaaccaa tcacgggcg attttaaag ctccttgac aagaactta gtcaatcgg gaatacgtg gaataccag ttcattcaa cagagccaa attccaacc tcacatttc caatcagca ttgccccga cttccgatt cctgaatta aaattaacg tgtgagcgg tacttggcg gctcagcgg cctttgtta atcgacgag tcgtacaaa aaaaccgaa gccaacgtc tatcgcttc cgggacggg gttggcaaa cagttacaa aaaatttta aaagactac gacaataag aacaagcac acgagttac ataagtgag ttgtggttc gacaagtat ctaagatcg cgaagtcct ttaccgcta aactgcaac ccggtcctg gttttaaac aacgacgat aaaccggaa tacaacaat cagctcatt agagccaca aatttgatt gtcagctct ttgcgattt taccgtgcc ttaaagtcg gagttgttg caccccgaa attttccac ttgaatccg aaaaaaagc gacacagag cggctgaac ttcgtcttg agtaaattg ccgccgaaa ttggcctac atttgtgcg tattttaac aaggcgttc cctttggac atgagtcaa tatccgagt ttgttccaa acgactcgt attcctgag actgataag gataggctt gttagttgt cccgagtct aaacacatt acagtgcag tacaaaggt cgcttctat tctttcgaa gtattaact gttagtaat gatatcgtc tcgcccacg caaatcatg gacaacatc aagttcatc ctgagtgat aagacagat tctgataag tttccaatt ggggtcctc acgaccctc gagcgcaac aaatgggcc acaattcgc cacgaactg gccgaaaac ggcaacgag gccctgctt aaaacaatc gattcggcc ctgtttcac gtctgtctg gacgaagaa acggtcgga agcgaccct tataaaata actaaacag tttttgcac gccgatggg gccaataga tggttcgat aattcgttt tcgctgatt gtggcgaga gatggcgcc gctgcggtc aatttcgag cacgcgtgg ggagatggc gttgctgtc ttacgttac gcacaagac atttacaat gatgcaaaa aataagcca agggtgcat cctgatacc gctctaacg gaggattct tcctgccaa gttcagcgt ctagaagtt catctgaat gatcatttg agaaaatgt gtcgatgat gcgaaaaat tcgtataaa acgatttgt gataatctt gatgtcaat tatacgatt tttgagggg attggaaag gatacgtgc aagaagagt caagtcagt ccagacgcg atcatgcaa ctggcgttc cagctctgc tgtgaaaaa caatcgccc atcgacgat ttgaactcg tcgattcgt cgcagtagt tttggttgg agtattgtg tccgccatt ataatggat gcgtatgaa gtggaatta tatggagat tatccgaat ttgaataat aacgaattg ccgtatata attggttgt ctacataca aaaaacatg gaggccgtc cagaagttt tttttgcgg ggcttatac aaagatgtg aagatacat caaatcact cgatggaaa agaaattgc cagccagta tgtgtgtat acggtggtt tacaaaatc aaggttagg actcccgat ttaagtgaa gaggaaatc ttcattcca tttccagat aatgaagaa gatttaaaa aaaatgttc cgtcatatt actgaaatg gtttttgga atgcgtgtg gtcgaaaaa aaactcagt gatgatgga gtcttcgca tcaataagc tatgccccg gttcgttgt gcatgcaat gtgcgaaat tttgatatg gctgcagag gagcctcgc atgcagccc ctaattaca gaggtggct tatcacaaa ataaatggg ctttttgtg tccacctac gagtcgtgc agcacttct gctttcaag cacggcagg acggaagca attcgtccg tgcacgacc gccaccaag gacgttaca ttggctatt aacgcgttg aataagcca ccggtggct caactgaaa aaaatgatt caggagtgt tcgacgatc cacaatagg ctgatcaag gaagctgcg atggggcaa ggatttgat cggcacctt ttcgccctt aaatcaatg ggcgaaaag aacgatatt aagagcaac atttttgac gatcctgag tattcgcac atgggttac gatatactc tcgacgagt actttgaac tcgccggcc atttttgcg gggggtttt ggaccagtg gtgccggaa gggttcgga ttggggtat gtgattaaa gacgagttt ttggggacg cttgcaacg aattataag gaccgtact aatggacca gacttcatg ggtgctttg acaaagtca ttcgaggaa atttttgca attttgcga accaattag ttaagcaat gatttatat ttattccgg tatttttag ttcgaattt gtattcttc tgtttttgg ttgtgtttc atacagtat gagtaaggt tgaatatta taaaattat ataattttt tatcgttcg ttaataaat gtttcacat tttc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
