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Gene information
General information
TC004777 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6603821..6612355
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004777
    iBeetle screen iB_03844
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature degenerated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    leg musculature not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: body wall muscle pattern ok. coxa musculature present.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    elytra sometimes colour decreased - Number of animals showing the phenotype: 6


    elytra sometimes surface irregular - Number of animals showing the phenotype: 6


    elytra dorsal orientation not closed - Number of animals showing the phenotype: 6


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004777 RA
      MVFPKTTPL QNQSRAILK LLDKNLVNR EYVEYQFIQ 
      QSQIPTSHF QSALPRLPI PELKLTCER YLAAQRPLL 
      IDESYKKTE ANVYRFRDG VGKQLQKIL KDYDNKNKH 
      TSYISELWF DKYLRSRSP LPLNCNPVL VLNNDDKPE 
      YNNQLIRAT NLIVSSLRF YRALKSELL HPEIFHLNP 
      KKSDTERLN FVLSKLPPK LAYICAYFN KAFPLDMSQ 
      YPSLFQTTR IPETDKDRL VSCPESKHI TVQYKGRFY 
      SFEVLTVSN DIVSPTQIM DNIKFILSD KTDSDKFPI 
      GVLTTLERN KWATIRHEL AENGNEALL KTIDSALFH 
      VCLDEETVG SDPYKITKQ FLHADGANR WFDNSFSLI 
      VARDGAAAV NFEHAWGDG VAVLRYAQD IYNDAKNKP 
      RVHPDTALT EDSSCQVQR LEVHLNDHL RKCVDDAKN 
      SYKTICDNL DVNYTIFEG IGKDTCKKS QVSPDAIMQ 
      LAFQLCCEK QSPIDDLNS SIRRSSFGW SIVSAIIMD 
      AYEVELYGD YPNLNNNEL PYIIGCLHT KNMEAVQKF 
      FLRGLYKDV KIHQITRWK RNCQPVCVY TVVYKIKVR 
      TPDLSEEEI FIPFPDNEE DLKKMFRHI TEMVFGMRV 
      VEKKLSDDG VFASISYAP VRCACNVRN FDMAAEEPR 
      MQPLITEVA YHKINGLFV STYESCSTS AFKHGRTEA 
      IRPCTTATK DVTLAINAL NKPPVAQLK KMIQECSTI 
      HNRLIKEAA MGQGFDRHL FALKSMGEK NDIKSNIFD 
      DPEYSHMGY DILSTSTLN SPAIFAGGF GPVVPEGFG 
      LGYVIKDEF LGTLATNYK DRTNGPDFM GALTKSFEE 
      IFAILRTN
    CDS sequence
    • >TC004777 RA
      atggtcttc cccaaaaca acacccctt cagaaccaa 
      tcacgggcg attttaaag ctccttgac aagaactta 
      gtcaatcgg gaatacgtg gaataccag ttcattcaa 
      cagagccaa attccaacc tcacatttc caatcagca 
      ttgccccga cttccgatt cctgaatta aaattaacg 
      tgtgagcgg tacttggcg gctcagcgg cctttgtta 
      atcgacgag tcgtacaaa aaaaccgaa gccaacgtc 
      tatcgcttc cgggacggg gttggcaaa cagttacaa 
      aaaatttta aaagactac gacaataag aacaagcac 
      acgagttac ataagtgag ttgtggttc gacaagtat 
      ctaagatcg cgaagtcct ttaccgcta aactgcaac 
      ccggtcctg gttttaaac aacgacgat aaaccggaa 
      tacaacaat cagctcatt agagccaca aatttgatt 
      gtcagctct ttgcgattt taccgtgcc ttaaagtcg 
      gagttgttg caccccgaa attttccac ttgaatccg 
      aaaaaaagc gacacagag cggctgaac ttcgtcttg 
      agtaaattg ccgccgaaa ttggcctac atttgtgcg 
      tattttaac aaggcgttc cctttggac atgagtcaa 
      tatccgagt ttgttccaa acgactcgt attcctgag 
      actgataag gataggctt gttagttgt cccgagtct 
      aaacacatt acagtgcag tacaaaggt cgcttctat 
      tctttcgaa gtattaact gttagtaat gatatcgtc 
      tcgcccacg caaatcatg gacaacatc aagttcatc 
      ctgagtgat aagacagat tctgataag tttccaatt 
      ggggtcctc acgaccctc gagcgcaac aaatgggcc 
      acaattcgc cacgaactg gccgaaaac ggcaacgag 
      gccctgctt aaaacaatc gattcggcc ctgtttcac 
      gtctgtctg gacgaagaa acggtcgga agcgaccct 
      tataaaata actaaacag tttttgcac gccgatggg 
      gccaataga tggttcgat aattcgttt tcgctgatt 
      gtggcgaga gatggcgcc gctgcggtc aatttcgag 
      cacgcgtgg ggagatggc gttgctgtc ttacgttac 
      gcacaagac atttacaat gatgcaaaa aataagcca 
      agggtgcat cctgatacc gctctaacg gaggattct 
      tcctgccaa gttcagcgt ctagaagtt catctgaat 
      gatcatttg agaaaatgt gtcgatgat gcgaaaaat 
      tcgtataaa acgatttgt gataatctt gatgtcaat 
      tatacgatt tttgagggg attggaaag gatacgtgc 
      aagaagagt caagtcagt ccagacgcg atcatgcaa 
      ctggcgttc cagctctgc tgtgaaaaa caatcgccc 
      atcgacgat ttgaactcg tcgattcgt cgcagtagt 
      tttggttgg agtattgtg tccgccatt ataatggat 
      gcgtatgaa gtggaatta tatggagat tatccgaat 
      ttgaataat aacgaattg ccgtatata attggttgt 
      ctacataca aaaaacatg gaggccgtc cagaagttt 
      tttttgcgg ggcttatac aaagatgtg aagatacat 
      caaatcact cgatggaaa agaaattgc cagccagta 
      tgtgtgtat acggtggtt tacaaaatc aaggttagg 
      actcccgat ttaagtgaa gaggaaatc ttcattcca 
      tttccagat aatgaagaa gatttaaaa aaaatgttc 
      cgtcatatt actgaaatg gtttttgga atgcgtgtg 
      gtcgaaaaa aaactcagt gatgatgga gtcttcgca 
      tcaataagc tatgccccg gttcgttgt gcatgcaat 
      gtgcgaaat tttgatatg gctgcagag gagcctcgc 
      atgcagccc ctaattaca gaggtggct tatcacaaa 
      ataaatggg ctttttgtg tccacctac gagtcgtgc 
      agcacttct gctttcaag cacggcagg acggaagca 
      attcgtccg tgcacgacc gccaccaag gacgttaca 
      ttggctatt aacgcgttg aataagcca ccggtggct 
      caactgaaa aaaatgatt caggagtgt tcgacgatc 
      cacaatagg ctgatcaag gaagctgcg atggggcaa 
      ggatttgat cggcacctt ttcgccctt aaatcaatg 
      ggcgaaaag aacgatatt aagagcaac atttttgac 
      gatcctgag tattcgcac atgggttac gatatactc 
      tcgacgagt actttgaac tcgccggcc atttttgcg 
      gggggtttt ggaccagtg gtgccggaa gggttcgga 
      ttggggtat gtgattaaa gacgagttt ttggggacg 
      cttgcaacg aattataag gaccgtact aatggacca 
      gacttcatg ggtgctttg acaaagtca ttcgaggaa 
      atttttgca attttgcga accaattag
    mRNA sequence
    • >TC004777 RA
      agagacaaa ctttttaat cattgtccc ttattaaag 
      ccttgtctt gattccacc ctttactta tctgaattc 
      gggggttgg tgcgagaaa tgacttgac agtctgtgt 
      ttgacaatg acagttcag tttgacagt agaatgtgt 
      aggtaaaat ttgttttgt tgaaatttt cctcatatt 
      tcttcaaaa tttagttta atttaaatg tagacacgt 
      gcccgtgct tcttttcgt aattagtta atttcgcag 
      tgtgtcagt taatcaaaa atggtcttc cccaaaaca 
      acacccctt cagaaccaa tcacgggcg attttaaag 
      ctccttgac aagaactta gtcaatcgg gaatacgtg 
      gaataccag ttcattcaa cagagccaa attccaacc 
      tcacatttc caatcagca ttgccccga cttccgatt 
      cctgaatta aaattaacg tgtgagcgg tacttggcg 
      gctcagcgg cctttgtta atcgacgag tcgtacaaa 
      aaaaccgaa gccaacgtc tatcgcttc cgggacggg 
      gttggcaaa cagttacaa aaaatttta aaagactac 
      gacaataag aacaagcac acgagttac ataagtgag 
      ttgtggttc gacaagtat ctaagatcg cgaagtcct 
      ttaccgcta aactgcaac ccggtcctg gttttaaac 
      aacgacgat aaaccggaa tacaacaat cagctcatt 
      agagccaca aatttgatt gtcagctct ttgcgattt 
      taccgtgcc ttaaagtcg gagttgttg caccccgaa 
      attttccac ttgaatccg aaaaaaagc gacacagag 
      cggctgaac ttcgtcttg agtaaattg ccgccgaaa 
      ttggcctac atttgtgcg tattttaac aaggcgttc 
      cctttggac atgagtcaa tatccgagt ttgttccaa 
      acgactcgt attcctgag actgataag gataggctt 
      gttagttgt cccgagtct aaacacatt acagtgcag 
      tacaaaggt cgcttctat tctttcgaa gtattaact 
      gttagtaat gatatcgtc tcgcccacg caaatcatg 
      gacaacatc aagttcatc ctgagtgat aagacagat 
      tctgataag tttccaatt ggggtcctc acgaccctc 
      gagcgcaac aaatgggcc acaattcgc cacgaactg 
      gccgaaaac ggcaacgag gccctgctt aaaacaatc 
      gattcggcc ctgtttcac gtctgtctg gacgaagaa 
      acggtcgga agcgaccct tataaaata actaaacag 
      tttttgcac gccgatggg gccaataga tggttcgat 
      aattcgttt tcgctgatt gtggcgaga gatggcgcc 
      gctgcggtc aatttcgag cacgcgtgg ggagatggc 
      gttgctgtc ttacgttac gcacaagac atttacaat 
      gatgcaaaa aataagcca agggtgcat cctgatacc 
      gctctaacg gaggattct tcctgccaa gttcagcgt 
      ctagaagtt catctgaat gatcatttg agaaaatgt 
      gtcgatgat gcgaaaaat tcgtataaa acgatttgt 
      gataatctt gatgtcaat tatacgatt tttgagggg 
      attggaaag gatacgtgc aagaagagt caagtcagt 
      ccagacgcg atcatgcaa ctggcgttc cagctctgc 
      tgtgaaaaa caatcgccc atcgacgat ttgaactcg 
      tcgattcgt cgcagtagt tttggttgg agtattgtg 
      tccgccatt ataatggat gcgtatgaa gtggaatta 
      tatggagat tatccgaat ttgaataat aacgaattg 
      ccgtatata attggttgt ctacataca aaaaacatg 
      gaggccgtc cagaagttt tttttgcgg ggcttatac 
      aaagatgtg aagatacat caaatcact cgatggaaa 
      agaaattgc cagccagta tgtgtgtat acggtggtt 
      tacaaaatc aaggttagg actcccgat ttaagtgaa 
      gaggaaatc ttcattcca tttccagat aatgaagaa 
      gatttaaaa aaaatgttc cgtcatatt actgaaatg 
      gtttttgga atgcgtgtg gtcgaaaaa aaactcagt 
      gatgatgga gtcttcgca tcaataagc tatgccccg 
      gttcgttgt gcatgcaat gtgcgaaat tttgatatg 
      gctgcagag gagcctcgc atgcagccc ctaattaca 
      gaggtggct tatcacaaa ataaatggg ctttttgtg 
      tccacctac gagtcgtgc agcacttct gctttcaag 
      cacggcagg acggaagca attcgtccg tgcacgacc 
      gccaccaag gacgttaca ttggctatt aacgcgttg 
      aataagcca ccggtggct caactgaaa aaaatgatt 
      caggagtgt tcgacgatc cacaatagg ctgatcaag 
      gaagctgcg atggggcaa ggatttgat cggcacctt 
      ttcgccctt aaatcaatg ggcgaaaag aacgatatt 
      aagagcaac atttttgac gatcctgag tattcgcac 
      atgggttac gatatactc tcgacgagt actttgaac 
      tcgccggcc atttttgcg gggggtttt ggaccagtg 
      gtgccggaa gggttcgga ttggggtat gtgattaaa 
      gacgagttt ttggggacg cttgcaacg aattataag 
      gaccgtact aatggacca gacttcatg ggtgctttg 
      acaaagtca ttcgaggaa atttttgca attttgcga 
      accaattag ttaagcaat gatttatat ttattccgg 
      tatttttag ttcgaattt gtattcttc tgtttttgg 
      ttgtgtttc atacagtat gagtaaggt tgaatatta 
      taaaattat ataattttt tatcgttcg ttaataaat 
      gtttcacat tttc