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Gene information
General information
TC004774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6793665..6797866
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004774
      iBeetle screen iB_03843

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004774 RA
        MDTPLIILV LAISMAPII STDAKTAAL KRPKREPPI 
        YRPIKHSYS PPPRRKPRP IYGPPRPVY GPPSKPKPV 
        YGPPKPVYG PPKPVYGPP KPVYGPPSY SYHAPAPAP 
        APAPSYGPP PSSYGAPVQ SYGPPEPSY GPPAPSYGP 
        PAQSYGPPA PSYGPPAPS YGPPEPSYG PPPAPSYGP 
        PPAQSYGPP AGHYSGYES QTTSYSHSS PSYGVPEVY 
        QPSYDIPPN FNSHPLTYD TSSASYGTP GALNSYGVP 
        ATQHEAPIQ HYDFSSYSH RTEEVPNEE FHASYSDPY 
        SADGSSNYV KFEESTKKL HAPDPTVSY GKSETTTKV 
        PNHNYDGPG VQDDSLVHY VNDRKKQLQ KLKNYYKFK 
        NSDYSTSDI EIQKSHSYD FYAGKSNGF GEKRNHYRQ 
        HHH
      CDS sequence
      • >TC004774 RA
        atggatact cctctcatc attctcgtt ttggccata 
        tctatggcc cccataatc tccaccgat gcgaagaca 
        gcagccctg aaacgccca aaacgcgaa cccccgatt 
        taccgcccc atcaaacac tcctacagc cccccgccc 
        cgtcgcaaa cctcgtccc atctacggc ccgcctcgt 
        cccgtctac ggccctccc agcaagcct aaaccggtt 
        tacggccca cctaaaccg gtttacgga ccgcctaaa 
        ccggtttat gggccacct aaaccggtt tatggacct 
        ccgtcttac agttaccac gcccctgcc cctgctcct 
        gcccctgcc cctagttac ggcccgcct ccgtcgtcg 
        tatggggcc ccagttcag tcatacgga ccgccggag 
        ccatcatat ggcccccca gcgccgtcg tatggtcct 
        ccagcgcag tcgtatggc cctccagcg ccgtcgtat 
        ggccctcca gcgccgtca tatgggccc ccagaacct 
        tcatatggg cccccaccg gcgccctcg tatggaccc 
        cctccagcg cagtcctat gggcccccg gccggtcat 
        tactcaggg tatgagtcc cagacaacg tcatatagt 
        cattcgtcg cccagttat ggcgttcca gaggtgtat 
        caaccgtca tatgacata ccgccgaat tttaacagt 
        catccattg acatatgac acgtcgtcg gcaagttat 
        ggcactccc ggggctttg aattcgtat ggcgtgccc 
        gcgacgcaa catgaagcc cccatacaa cactatgac 
        ttctcgtca tacagtcat agaaccgag gaagtcccc 
        aatgaggag tttcacgca tcatattcc gatccatac 
        agtgctgat ggcagcagc aattatgtc aaatttgag 
        gaaagtacc aaaaaacta cacgcgcca gatcccaca 
        gtgtcatat ggcaaaagt gaaaccacg accaaagtg 
        ccaaaccat aattatgac gggccggga gtacaagat 
        gactctctt gtacattat gtcaatgac aggaaaaaa 
        caacttcaa aaactgaaa aattactac aagttcaaa 
        aactctgac tattccacg agtgacata gaaatacaa 
        aagtcgcac tcttatgac ttctatgcc ggaaaatcc 
        aacggtttc ggcgagaaa cggaaccat tacaggcaa 
        caccaccac tag
      mRNA sequence
      • >TC004774 RA
        cctgcccat tcactggcg ctcggctgg agtggtgta 
        gtgtggttc ggtattgtt tatttgtgc tctgtggtg 
        gacacaact tacacaaaa atcatggat actcctctc 
        atcattctc gttttggcc atatctatg gcccccata 
        atctccacc gatgcgaag acagcagcc ctgaaacgc 
        ccaaaacgc gaacccccg atttaccgc cccatcaaa 
        cactcctac agccccccg ccccgtcgc aaacctcgt 
        cccatctac ggcccgcct cgtcccgtc tacggccct 
        cccagcaag cctaaaccg gtttacggc ccacctaaa 
        ccggtttac ggaccgcct aaaccggtt tatgggcca 
        cctaaaccg gtttatgga cctccgtct tacagttac 
        cacgcccct gcccctgct cctgcccct gcccctagt 
        tacggcccg cctccgtcg tcgtatggg gccccagtt 
        cagtcatac ggaccgccg gagccatca tatggcccc 
        ccagcgccg tcgtatggt cctccagcg cagtcgtat 
        ggccctcca gcgccgtcg tatggccct ccagcgccg 
        tcatatggg cccccagaa ccttcatat gggccccca 
        ccggcgccc tcgtatgga ccccctcca gcgcagtcc 
        tatgggccc ccggccggt cattactca gggtatgag 
        tcccagaca acgtcatat agtcattcg tcgcccagt 
        tatggcgtt ccagaggtg tatcaaccg tcatatgac 
        ataccgccg aattttaac agtcatcca ttgacatat 
        gacacgtcg tcggcaagt tatggcact cccggggct 
        ttgaattcg tatggcgtg cccgcgacg caacatgaa 
        gcccccata caacactat gacttctcg tcatacagt 
        catagaacc gaggaagtc cccaatgag gagtttcac 
        gcatcatat tccgatcca tacagtgct gatggcagc 
        agcaattat gtcaaattt gaggaaagt accaaaaaa 
        ctacacgcg ccagatccc acagtgtca tatggcaaa 
        agtgaaacc acgaccaaa gtgccaaac cataattat 
        gacgggccg ggagtacaa gatgactct cttgtacat 
        tatgtcaat gacaggaaa aaacaactt caaaaactg 
        aaaaattac tacaagttc aaaaactct gactattcc 
        acgagtgac atagaaata caaaagtcg cactcttat 
        gacttctat gccggaaaa tccaacggt ttcggcgag 
        aaacggaac cattacagg caacaccac cactagata 
        tttattacc atttttgta aatatttat gctacacaa 
        tatcttacc atatttatt gtactgata ctaccaaag 
        aacgaataa aatgtttac ctgacg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY