| General information | |||
| TC004772 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6772265..6779621 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004772 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03842 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC004772 RA
MSGPDVDEV LNYLSIKFN KIFCDREFV CCVIEDFQA VLGNKARIC VLVFPTLGP IYLKALRYQ ARIFDLAVF NITSASGKF RSLAVCHKD HLKKQSKEE AMPESSKRK RPERQLYVP PAQRGARKS DKKVTEPVP KPSAISDEC LLNLSDVLL LYHNFIKIY QLVLSDVYW DLNQFQVIK PGFYFQTCP VYDFENLQT VAEKPSAVL VDQEVSDFA QVVNGLPEF ENSLYRIEC DNVCDSENC IVDLSVIGL GHFFIISME NSDYFYECQ EQHDPSICC ANVRNQLAT SKKLERLHI SAIKSENET NKNGTPKSN KDVIKNQIN MNKKKVSDN THEQEKEIM RMTKEYINR KTRPIMKYV DDSNDTLRI DKSDSVNNW EDLFDDNGQ IQEELLTEI VDKVGDDIT IVKASEDYT AYTNKPPEE LEHVVELYN FPPTLETHD LVQAFSEIN SDAMYIKWV DDTHALLVL GSLSQAQKA VSMRNPLIK VRPMTAASA ISMDVANKS DLKPAMKRP PTNLQTARR LITSHLGTK SKLTKEQIA KEKQDLKNA REMKRMLKQ NEKDAWEGN LRSSVN
- >TC004772 RA
atgagcggg cccgatgtg gatgaggtg ctaaactac ttgtcgata aaattcaac aaaatcttc tgtgatcgg gagttcgtg tgttgtgta attgaagac ttccaggca gttttgggg aataaggcc cgaatttgt gtcctcgtc ttcccaacg ttggggcca atctacctt aaagcgctt cgctaccaa gcgaggatt ttcgacttg gctgtcttt aatataact tcggcctcc ggcaagttc aggtcgctt gcagtatgc cataaggac catttaaag aagcaaagc aaagaagaa gcaatgcca gaatcatcc aaacgcaaa cgtccagaa cgccagctc tacgtaccc ccagcacag aggggggcc cgcaaaagt gacaaaaaa gtgaccgaa cccgttccc aaaccgagt gcaatcagt gacgagtgt ttgctaaat ctgagtgat gtgcttttg ctgtatcac aacttcatc aaaatttac caactggtt ctgagcgac gtctattgg gatttaaac cagttccag gtcataaaa ccggggttt tattttcaa acttgccca gtttatgat tttgaaaac cttcaaacc gtggctgag aaaccttca gccgtttta gtcgatcaa gaagtgagc gattttgca caagtcgtg aacggattg cctgaattt gaaaatagt ctatacaga atagagtgt gataatgtt tgtgattct gaaaattgc atagtggat ttatcagtg ataggatta ggacatttc ttcattatt tcgatggaa aatagtgat tatttttac gagtgccaa gagcaacac gacccatct atttgctgt gcaaatgtt cgaaaccag ttagcaact agtaaaaaa ttagagaga ttacacatt agtgcaata aagagcgaa aatgaaacg aataaaaac ggaacacca aaaagcaac aaagacgtt atcaaaaat caaataaac atgaataag aaaaaagtg tcggataac acccacgaa caggagaag gagataatg cgaatgact aaggaatac atcaatcgg aagactcgg cctattatg aaatatgtg gacgattct aacgacacg ttacgcatt gataagagc gacagtgtc aataactgg gaggacctc tttgatgac aacggccag attcaggaa gaattactg actgaaatc gttgataag gtcggcgat gatataaca atagtgaaa gccagcgaa gactacacg gcttacaca aacaagccc ccggaagag ctggagcac gtagttgaa ctttacaac tttcctcca accctcgaa acccacgat ttggtccaa gctttcagc gagattaac agcgatgcc atgtacata aaatgggtc gacgatacc catgctttg cttgtttta ggctctctt agccaagct caaaaagcg gtgagtatg cggaacccc ctgatcaaa gttcgtcca atgaccgca gcgagcgct atatccatg gatgtggcc aacaagtcg gatttaaaa ccggcgatg aaacgtcca ccgactaat ttgcagact gcgaggaga ttaatcact tctcattta ggtaccaaa agtaaatta acgaaagaa cagatcgcg aaagaaaaa caggatttg aaaaacgcc agagaaatg aaacggatg ctcaaacaa aacgaaaaa gacgcctgg gagggcaat ctgcgatcg tcagtcaac tag
- >TC004772 RA
tttcaattt catttccac aaaattcac atgacattg cacgtttgt tattacgtt ttatcgttt tttgtttgt gtttaaata aataaaagt acaaaaaca atgaccttt ggctattat gagcgggcc cgatgtgga tgaggtgct aaactactt gtcgataaa attcaacaa aatcttctg tgatcggga gttcgtgtg ttgtgtaat tgaagactt ccaggcagt tttggggaa taaggcccg aatttgtgt cctcgtctt cccaacgtt ggggccaat ctaccttaa agcgcttcg ctaccaagc gaggatttt cgacttggc tgtctttaa tataacttc ggcctccgg caagttcag gtcgcttgc agtatgcca taaggacca tttaaagaa gcaaagcaa agaagaagc aatgccaga atcatccaa acgcaaacg tccagaacg ccagctcta cgtaccccc agcacagag gggggcccg caaaagtga caaaaaagt gaccgaacc cgttcccaa accgagtgc aatcagtga cgagtgttt gctaaatct gagtgatgt gcttttgct gtatcacaa cttcatcaa aatttacca actggttct gagcgacgt ctattggga tttaaacca gttccaggt cataaaacc ggggtttta ttttcaaac ttgcccagt ttatgattt tgaaaacct tcaaaccgt ggctgagaa accttcagc cgttttagt cgatcaaga agtgagcga ttttgcaca agtcgtgaa cggattgcc tgaatttga aaatagtct atacagaat agagtgtga taatgtttg tgattctga aaattgcat agtggattt atcagtgat aggattagg acatttctt cattatttc gatggaaaa tagtgatta tttttacga gtgccaaga gcaacacga cccatctat ttgctgtgc aaatgttcg aaaccagtt agcaactag taaaaaatt agagagatt acacattag tgcaataaa gagcgaaaa tgaaacgaa taaaaacgg aacaccaaa aagcaacaa agacgttat caaaaatca aataaacat gaataagaa aaaagtgtc ggataacac ccacgaaca ggagaagga gataatgcg aatgactaa ggaatacat caatcggaa gactcggcc tattatgaa atatgtgga cgattctaa cgacacgtt acgcattga taagagcga cagtgtcaa taactggga ggacctctt tgatgacaa cggccagat tcaggaaga attactgac tgaaatcgt tgataaggt cggcgatga tataacaat agtgaaagc cagcgaaga ctacacggc ttacacaaa caagccccc ggaagagct ggagcacgt agttgaact ttacaactt tcctccaac cctcgaaac ccacgattt ggtccaagc tttcagcga gattaacag cgatgccat gtacataaa atgggtcga cgataccca tgctttgct tgttttagg ctctcttag ccaagctca aaaagcggt gagtatgcg gaaccccct gatcaaagt tcgtccaat gaccgcagc gagcgctat atccatgga tgtggccaa caagtcgga tttaaaacc ggcgatgaa acgtccacc gactaattt gcagactgc gaggagatt aatcacttc tcatttagg taccaaaag taaattaac gaaagaaca gatcgcgaa agaaaaaca ggatttgaa aaacgccag agaaatgaa acggatgct caaacaaaa cgaaaaaga cgcctggga gggcaatct gcgatcgtc agtcaacta ggttaagtt ttgtgattt gtgcttaaa tgtgtacct agagtcaaa tttatagtc attttttgt agtttttac aagtttttg gttaccaag atacgtagg taatgtttt atttattgt tttgtactc tttttttgc ttcctgtgg aataagaga agtttgagg aagtgacct taatatttt attttgtga tattcaaca ttttagaaa aatattctc tacatttgc gaacatctc aggatcgat cataatttg gttatgtgt tctttgaaa ctgaatata ttttattta ctctt
