| General information | |||
| TC004768 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6662390..6672824 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004768 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03840 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
labium proximal shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
labium proximal shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
Days post injection
Fertility
egg production: number no/almost no eggs -
tropharium shape irregular - Number of animals showing the phenotype: 2
tropharium shape irregular - Number of animals showing the phenotype: 2
- >TC004768 RA
MGRSKFPGK PSKHVNRKR INVLPPTSE GIIESSRSM TVETVAENK QAENDVEES SESVNLNNT INTKVSLRR RLNRKMQSS KALMSNNSS KRGLISKLC NKNSVSCKL SVKSKLNCK ASRNRKSVH AYSPIKNFR QCRSGITKE SNNLVGKFV LPTRSVHSS RVIKPNKRF INVDLSDTL TLKKRVINK RHCIKQSEN PSKTNKSDS ASESPTKTF SNGRVVLRQ ARLKLHNQT PKGSEGPFS SNATNGSPP GTVTCGVCG AVRFYRFVK QARKFNIYS CESCRKFIS KMIKRQSCG KNLSSATLV CHKGQGMCH VPPIVRSQQ MKLIRCAYK SRCPACWLK MCLRSFQMP QSLKNSLIQ LLPMNMQGS DVMFNNSLP LLWQVSVQN INKPEQNKT VNQRPVRFK NTKPVTQPT LAPPTSDIK RQKIDLKGP RVKHVCRSA SIVLGQPLA MFPSETEKK RTEECPKKA QKTSSTHAE NVETPPQVK SLKDVECSE NESDNDKSI KVPLVLNEN LISQIKRDR SHRVPFSSV SSSSSLPSF PSLSSSSFT SLSESKSDF EKKPNLQEV QNMISINYW DHYDLDDVK QNGFCLIAS QQFPMPAIC FLCGSAGRE ALLHCSLCC EPYHPFCLE RSPPFSSTS NNNRQNVWL CPRCTTCNA CNRADRQKI HCQKCSKAY HSECLKTDW TGKGKPTIC SKCLSCKDC GDLNTTIFF GNSPLCVTC FKLRRKGSC PVCLIPFRE IDTNEKMME CTKCKKWIH ADCEDLSEE YYHILSYLP ESVKYLCCA CSKQSPPFW RKSIESELK YNFNRVLRL LSKNKTAKN MLKLSLLNN YVTLTRTTG KKSDCENDG DDNQENLAT YFNSFNKNS DNEAPLKSF SDFKIAQTR TLTVLDIKR KANSNEYSS VKDFHKEME EGLAQTQSD ELLNIYRII LKSVFPWFD ISQEDSKWN NTKQRFLCN NNIDEDICG QIECVSIDS RYCSFCKSI GDGLSHLES RLLYCGQNE WVHINCALW SSEVYEEID GSLQNVHSA LSRGRLMRC SYCKQKGAT VGCCFKNCC EIYHFICAR TAKCHFMHD KTVFCCSHE VPKTSSVIV TPHEFNIRR SVFVELADY KKEQYSQIE KVNVVVGSL CVTNLGKIN PVMSDTVEV IIPVGYNCS RLFWSTVEP WKLVSYKIS TSILNAHVN NHHVDKSFT VDHSLPKSV VERKLKEIL LWHKDIDKK KLDVIEFDD EEEPQNGAD LLSPEITDA IFEELPHEL LDGISVQDI FPKLMSYED FISMDSKSE TTNSDNNGN TELKKSDQE VENVEEFDD KEFKSNKNP QKTCSLTLS CKLDNSFSP VIKKRKITP RENNLFFQL LQVDGNFDD SSSDCGSPV GVNSVNIWD SDVSEEPVT CERCQCTYR TQASYKRHL DACEVICTS ESDSELNQD QDANLETTQ IHINETTVN TELPQPVLI TSYESYQNE IHTSVLNTQ TFVASKSNA EVITVPQNP EIIIPQTEQ PITEPIVSS EVFTVSQST VSVNEQTSQ PFCLNPTVS LCVNPPLTL QPNATISVN PTQSVNPII NQTIAINQV TQPATVQPH QLEFQQPGV TIQSMPYST NVAPLINVT PQNQITLTD KLLNNSSII NSMINVPGN RCVIPSKSP TKVFKGRRS IAAKRGHEE ETIVIPASS TPVIVQHLP SSNFIPTFV DTFQQQSGQ NVQYVTTIA PQINSTINP QPLMQLQAD SNLISLLPG IQPTMIIQQ PRVLENQLI VDGNGSLSW TPPQVQPVY YGFETIVQN TVMQSQQFL PTTVPGVLT ANSSYSTTT QVFQTSKLE PVLDMGTNS FVLLNTGQL VNSQSLEVP QNSLTNVLQ QTEINQCKY STPSFQKPN SQPISVTNT NSITLPTAP FVPEQGIPT NIVTPTPKP VPTTQSRPM SRVLPMQTK EPNKVKTPT EKVEAIRKE IKILEKAVE PVVDFDKKN PIESTDKKL IETQLENDI NEAKKDSPL KLVFQKQTQ DGSYKVSNN VEEPLIPAM TNQISPKVA SNFKSTEPS YKLNNNFNN RNSPVQIAP LKPIKSNFN PPKPLHILS LSDKQNRPE KIEKRLAIE PPKHFPLEK KENENVPSI LYTIETQDG FRYSSTSIA DLWTKVFEA VQNARAAHN MPLLPSDST NIVNSLQIL GLKTSGLKY LIEQLPGAT KCSKYRPMF NFPSKCIEI EDELVGHTN GSARCAPHI KRNEPYDMF GWLASKHRK PEHSLFCSD LVPRRGSVN NLPMAMRFR QLKLTSKYS VGVYRSKIH RRGLFCLRD FEAGEMVIE YSGEVIRSV LTDKREKYY NSKGIGCYM FRIDDNLVV DATMTGNAA RFINHSCDP NCYSKVVEI LGHKHIIIF ALRRIICGE ELTYDYKFP IEEDKIPCT CGTRRCRKF LN
- >TC004768 RA
atgggccgt tcaaaattt cccggaaaa ccatcgaaa catgttaat aggaaaaga attaatgtt cttccaccc acctcggag ggaattatt gaatctagc aggtctatg acagttgaa actgttgca gaaaacaaa caggccgaa aatgatgta gaagaaagc agtgaaagt gtaaattta aacaatact ataaataca aaagtgtca ttgagaaga agactgaac cgaaaaatg caaagttcg aaagcattg atgagcaat aattcgtct aaacgtggt ctaatatca aaactctgt aataaaaat agtgtttcc tgcaaactc tcggttaaa agcaaatta aattgcaaa gcatcaagg aatcgcaaa tcagtgcat gcatactct ccaataaaa aatttccgc cagtgtcga agtggaata acaaaagaa agtaataat ctcgttgga aaattcgta ttacccaca agaagtgtc cattcatcg cgtgtgata aaaccaaac aaaagattc ataaacgtg gatttgagt gacacatta accctaaaa aagcgcgtc attaacaaa agacattgt ataaaacaa agcgaaaat ccgtcgaaa accaacaaa agtgacagt gcatcagaa tcgccaaca aaaacattc tcaaacggg cgcgtcgtt ctccgtcaa gcccgcttg aagctccac aatcaaacc cctaaaggc tctgaaggt ccgttttcc agcaatgca acaaacggc tctccgcca gggacagtg acttgtggt gtctgtggt gccgtccgt ttctaccga ttcgtgaag caagcgcgt aaatttaac atctacagc tgcgaatca tgccgcaaa tttatctca aaaatgata aaacggcag tcttgcggc aagaaccta agcagtgcc actctggtc tgccacaag ggacaagga atgtgtcac gtacccccc atagtgcgc agccaacaa atgaaactt atacggtgc gcctacaag tcccgatgc cctgcctgt tggctgaaa atgtgcctt cgctccttc caaatgccc caatctttg aaaaacagt ctaatccaa ctacttcca atgaacatg caaggctct gacgttatg ttcaacaac tcgctgccc ttgttatgg caggtcagc gttcagaat attaacaaa cctgaacag aacaaaacg gtaaatcag cgacctgtc aggtttaaa aacactaag cctgtcaca caacctact ctcgcacct ccaacgtct gatattaag aggcaaaaa attgatttg aaaggcccg cgagtgaaa cacgtttgt cgcagtgct tctatagtc ttgggccag cccctggcc atgtttccg agtgaaacc gagaaaaag agaactgag gaatgcccg aaaaaagca caaaagacg agttcaacg catgctgaa aacgttgaa acgccgcca caagtcaag tctttaaaa gatgtagaa tgcagcgaa aatgaatcc gataatgat aaatcaata aaggtgcca ttagtacta aatgaaaat ttaatttct caaattaag agggacagg agccataga gtgcctttt tcatccgta tcttcgtct tcgtcactt ccgtctttc ccttcactt tcatcgtca tcgtttacg tctttgagt gaaagcaaa tccgacttt gagaagaaa cccaatcta caagaagtc caaaatatg atctcaatt aattattgg gatcattac gacttggat gacgtcaag caaaatgga ttttgtctc attgcctct caacaattt ccaatgcct gctatttgt tttctttgt ggcagtgcg ggacgcgaa gcactttta cattgttct ctctgttgt gagccatat caccccttt tgtcttgaa agaagtcct ccatttagt agcaccagc aataataac agacaaaat gtgtggctc tgtccaaga tgtaccact tgcaacgct tgtaatcga gcagacagg caaaaaatc cattgtcag aagtgttcc aaagcttat cattcggaa tgtctaaag acagactgg accggcaaa ggtaaaccc acgatttgt tcgaaatgt ctaagttgt aaagactgc ggagattta aacactaca atttttttt gggaattca ccattatgt gtaacctgc tttaaactt cggaggaaa gggtcatgt cctgtttgt ttgatacca tttagagaa atcgataca aacgaaaag atgatggaa tgtacaaaa tgtaaaaag tggattcac gcggattgt gaggattta tcagaagag tattatcac atcttaagt tatctcccc gagtctgtt aaatacttg tgttgtgcg tgttcaaaa caatcgccg cctttttgg cgtaaatca atagagtcc gaactaaaa tataatttc aatcgagtg ttacggtta ctaagtaaa aacaaaaca gctaaaaat atgttaaag ttaagtctt ttaaataat tacgttacg ttgacaaga actaccggt aagaaaagt gattgcgag aatgacggc gatgacaat caggagaat ttagctaca tattttaat agttttaat aagaatagt gacaatgag gcacccttg aagagtttc agtgatttt aaaatcgcc caaacaagg acattaact gtgttagat attaaacgt aaagcgaac tcgaatgaa tatagttca gtgaaagac tttcataaa gaaatggaa gaagggtta gctcagaca caatcggat gagttgcta aatatctat cgaataatt cttaaatca gtattccct tggtttgat atttctcag gaagactcg aaatggaat aatacgaaa caacgattt ttatgtaat aataacatt gatgaagac atttgtggt caaatcgaa tgcgtgtca atagactcg agatattgt tcgttctgt aaaagtata ggtgatggt ttaagccat cttgaatct cgattactt tactgcggg caaaatgaa tgggtacac ataaactgt gctttatgg tcttctgaa gtctacgaa gaaatcgat ggttccctc caaaacgtc cacagtgca ctttcaaga ggtcgtcta atgcgttgc tcctactgt aaacaaaaa ggagcaacg gttggttgt tgtttcaag aactgttgc gagatttat cacttcatt tgcgcgaga actgcgaaa tgtcatttt atgcatgac aagactgta ttttgttgt agccacgaa gtaccgaag actagtagt gttatagtc acacctcat gagtttaat attcgtaga tctgttttt gtggaattg gctgattac aaaaaggag caatattca caaattgaa aaagttaac gtagtggtt gggtcactc tgtgttaca aatcttgga aaaataaat ccagttatg tctgacact gttgaggtg atcattccg gtcggttat aattgttct aggctcttt tggtcaact gtcgagcct tggaaatta gtttcgtat aaaatatct acttcgatt ttaaatgct catgttaat aatcaccac gttgataag agtttcacc gttgatcat tcccttcca aagtcagtg gttgaacgt aaattgaag gaaatcctc ctttggcac aaggatata gataagaaa aagctggat gttattgag tttgacgat gaggaggaa cctcaaaac ggggcagat cttttgtca cccgaaatc acagatgca atttttgag gaactcccc cacgagctg cttgatggt atttcggtg caagacatt tttccgaaa ctcatgtct tacgaggat tttattagt atggattca aaatcggaa acgaccaac tccgacaat aacggtaat actgaattg aagaaatca gaccaggaa gttgaaaat gtggaagag tttgacgat aaggagttt aaatcgaac aagaaccca caaaaaacg tgtagtctg actctcagt tgcaaactg gataacagc ttttctcca gtcattaag aaacggaaa ataactccg cgtgaaaat aacttattt tttcaattg ctgcaagtc gatggtaac tttgatgac agctcgagt gattgtggg tcaccagta ggggttaat agtgtaaac atatgggac tcggatgtg tctgaagaa ccggtcacg tgtgaacgt tgccaatgt acttaccgg acacaagcg agctacaaa cgacacttg gacgcatgc gaagttatt tgtacaagc gaaagcgat tcggaattg aatcaagat caagatgcg aatcttgaa acaactcaa attcacatc aacgaaaca acagtcaac accgaactt ccacaacct gttctcatc acctcatac gaatcttat caaaacgaa attcatacc tctgtttta aatactcaa actttcgta gcgtcgaaa tcaaatgca gaagtgatc actgttcct caaaatccg gagataatt attccgcag accgaacaa ccaataacc gaaccgatt gttagtagt gaagtgttc acagtatcg caatcgact gtgtcagta aatgaacaa acctcgcaa ccgttctgt ctaaacccg actgtctct ctttgtgtt aatccacct ctaacgtta caaccaaac gcaactatt tcggttaat ccgacacag tcggttaat ccgattata aaccaaact atcgctatt aaccaagtg actcaacca gcgactgta caaccgcat cagttggag tttcaacag ccaggagtt acaatacaa tccatgccg tactccact aatgtagct cctctcatt aatgtaaca ccccagaat cagattacc ctcaccgat aagttattg aataattca agtattata aattcgatg attaatgta cctggaaat cgatgtgtt ataccgtcg aaaagccca acaaaggtt tttaaagga agacgatcg attgctgcc aaaagaggt cacgaggag gaaaccatt gtgatacca gcaagttcg actccagtg attgttcaa cacttgcca tctagtaat tttattccg acttttgtt gacaccttc cagcaacaa tcaggacaa aatgtacaa tacgttaca acgattgca cctcaaatc aactcaacg atcaaccca caaccgctg atgcagtta caggctgac agtaatttg ataagtttg ttgcctggc attcaaccg acgatgatc attcaacag ccacgagtt ttggaaaat caattgatt gtggacggg aatggttca ttgagttgg acaccacca caagtacaa ccggtctat tacggtttt gagacgata gtacagaac actgtaatg cagtctcaa cagttttta ccaacaaca gtaccagga gtcttaact gctaacagc agttattct acaacgaca caagtattc caaactagt aaattagaa ccagtatta gatatgggg acgaatagc tttgtcctt ctaaataca ggacaattg gtcaattcg cagtcactc gaagtacct cagaattca ctaacgaat gttttacaa caaacagaa atcaatcag tgtaaatac tcaacacct tcatttcaa aaaccgaat agtcaacca attagtgtc actaataca aacagtata acactacct acggcaccc tttgtccca gaacaaggg ataccaact aatattgtt acgccaaca cctaaaccg gtaccaacc actcagagt cggccaatg agtcgcgtc ctacctatg cagacgaaa gaaccaaac aaagttaaa acaccaaca gaaaaagtt gaagcaatt agaaaagaa attaaaatc ctggagaag gccgtagaa ccagtagtc gattttgat aaaaagaac cctattgag agtaccgac aaaaaactc atcgaaact caattagaa aatgatata aatgaagcc aagaaagac tctcctttg aaactcgta ttccaaaaa cagacccaa gacggttcg tacaaagtc agtaacaac gtcgaggaa cctcttata cctgcaatg actaaccaa atcagcccc aaagtagcc agcaacttc aaatccact gaaccctcg tataaacta aacaacaat ttcaacaat cgcaactct ccagtacaa atagcacct ttaaaaccg atcaagtcg aacttcaac cccccaaaa ccactccac attctgtca ctgagtgac aaacaaaac cggcctgaa aaaatcgag aagaggctt gccatcgaa cctccaaag cacttcccg ctcgagaaa aaggagaat gagaacgtc ccgtccata ctttacaca attgagaca caggatggc ttccggtac tcatcaacg tccattgcg gacttgtgg accaaagtt ttcgaagct gtgcaaaac gcacgggcg gctcacaac atgccgctt ttgccctca gactcgaca aatattgta aacagtttg caaattctc ggcttgaag acttccgga cttaagtat ctcattgag caattgcca ggtgccacc aagtgctcg aagtatagg ccaatgttc aatttccca tcaaagtgt atagaaatc gaggacgag cttgtcggg catacaaac ggaagtgcg aggtgtgcg ccacacatc aaaagaaat gaaccctac gatatgttc gggtggtta gcgtcgaaa caccggaaa cctgaacat agtctattc tgttcagat ttagtgcca agaaggggc tctgtaaat aatttacca atggccatg cgattccga caattgaaa cttactagt aaatattca gtaggagta taccgttca aaaatccac cgacgaggc ttattctgt ttgagagat tttgaagca ggagaaatg gttatagaa tattcagga gaagttatt agatcagtt ttgactgat aaaagagaa aagtattat aattcgaaa ggaattggt tgttacatg tttcgaatt gacgataac ttggttgtt gatgcaacc atgaccgga aatgcggcc aggtttata aatcattct tgtgatccg aattgttat tcgaaagtc gttgaaatt ttgggtcac aagcacatc atcattttt gctttaaga agaatcatt tgtggagaa gaattaaca tacgattac aaatttccc atcgaggaa gataaaatt ccatgcact tgtggaaca cgacgttgt aggaaattt ctcaactga
- >TC004768 RA
gatgcgttg attgtagat tatcctatc tttaaaatg taaataata aataaaaaa acaaaatgg gccgttcaa aatttcccg gaaaaccat cgaaacatg ttaatagga aaagaatta atgttcttc cacccacct cggagggaa ttattgaat ctagcaggt ctatgacag ttgaaactg ttgcagaaa acaaacagg ccgaaaatg atgtagaag aaagcagtg aaagtgtaa atttaaaca atactataa atacaaaag tgtcattga gaagaagac tgaaccgaa aaatgcaaa gttcgaaag cattgatga gcaataatt cgtctaaac gtggtctaa tatcaaaac tctgtaata aaaatagtg tttcctgca aactctcgg ttaaaagca aattaaatt gcaaagcat caaggaatc gcaaatcag tgcatgcat actctccaa taaaaaatt tccgccagt gtcgaagtg gaataacaa aagaaagta ataatctcg ttggaaaat tcgtattac ccacaagaa gtgtccatt catcgcgtg tgataaaac caaacaaaa gattcataa acgtggatt tgagtgaca cattaaccc taaaaaagc gcgtcatta acaaaagac attgtataa aacaaagcg aaaatccgt cgaaaacca acaaaagtg acagtgcat cagaatcgc caacaaaaa cattctcaa acgggcgcg tcgttctcc gtcaagccc gcttgaagc tccacaatc aaaccccta aaggctctg aaggtccgt tttccagca atgcaacaa acggctctc cgccaggga cagtgactt gtggtgtct gtggtgccg tccgtttct accgattcg tgaagcaag cgcgtaaat ttaacatct acagctgcg aatcatgcc gcaaattta tctcaaaaa tgataaaac ggcagtctt gcggcaaga acctaagca gtgccactc tggtctgcc acaagggac aaggaatgt gtcacgtac cccccatag tgcgcagcc aacaaatga aacttatac ggtgcgcct acaagtccc gatgccctg cctgttggc tgaaaatgt gccttcgct ccttccaaa tgccccaat ctttgaaaa acagtctaa tccaactac ttccaatga acatgcaag gctctgacg ttatgttca acaactcgc tgcccttgt tatggcagg tcagcgttc agaatatta acaaacctg aacagaaca aaacggtaa atcagcgac ctgtcaggt ttaaaaaca ctaagcctg tcacacaac ctactctcg cacctccaa cgtctgata ttaagaggc aaaaaattg atttgaaag gcccgcgag tgaaacacg tttgtcgca gtgcttcta tagtcttgg gccagcccc tggccatgt ttccgagtg aaaccgaga aaaagagaa ctgaggaat gcccgaaaa aagcacaaa agacgagtt caacgcatg ctgaaaacg ttgaaacgc cgccacaag tcaagtctt taaaagatg tagaatgca gcgaaaatg aatccgata atgataaat caataaagg tgccattag tactaaatg aaaatttaa tttctcaaa ttaagaggg acaggagcc atagagtgc ctttttcat ccgtatctt cgtcttcgt cacttccgt ctttccctt cactttcat cgtcatcgt ttacgtctt tgagtgaaa gcaaatccg actttgaga agaaaccca atctacaag aagtccaaa atatgatct caattaatt attgggatc attacgact tggatgacg tcaagcaaa atggatttt gtctcattg cctctcaac aatttccaa tgcctgcta tttgttttc tttgtggca gtgcgggac gcgaagcac ttttacatt gttctctct gttgtgagc catatcacc ccttttgtc ttgaaagaa gtcctccat ttagtagca ccagcaata ataacagac aaaatgtgt ggctctgtc caagatgta ccacttgca acgcttgta atcgagcag acaggcaaa aaatccatt gtcagaagt gttccaaag cttatcatt cggaatgtc taaagacag actggaccg gcaaaggta aacccacga tttgttcga aatgtctaa gttgtaaag actgcggag atttaaaca ctacaattt tttttggga attcaccat tatgtgtaa cctgcttta aacttcgga ggaaagggt catgtcctg tttgtttga taccattta gagaaatcg atacaaacg aaaagatga tggaatgta caaaatgta aaaagtgga ttcacgcgg attgtgagg atttatcag aagagtatt atcacatct taagttatc tccccgagt ctgttaaat acttgtgtt gtgcgtgtt caaaacaat cgccgcctt tttggcgta aatcaatag agtccgaac taaaatata atttcaatc gagtgttac ggttactaa gtaaaaaca aaacagcta aaaatatgt taaagttaa gtcttttaa ataattacg ttacgttga caagaacta ccggtaaga aaagtgatt gcgagaatg acggcgatg acaatcagg agaatttag ctacatatt ttaatagtt ttaataaga atagtgaca atgaggcac ccttgaaga gtttcagtg attttaaaa tcgcccaaa caaggacat taactgtgt tagatatta aacgtaaag cgaactcga atgaatata gttcagtga aagactttc ataaagaaa tggaagaag ggttagctc agacacaat cggatgagt tgctaaata tctatcgaa taattctta aatcagtat tcccttggt ttgatattt ctcaggaag actcgaaat ggaataata cgaaacaac gatttttat gtaataata acattgatg aagacattt gtggtcaaa tcgaatgcg tgtcaatag actcgagat attgttcgt tctgtaaaa gtataggtg atggtttaa gccatcttg aatctcgat tactttact gcgggcaaa atgaatggg tacacataa actgtgctt tatggtctt ctgaagtct acgaagaaa tcgatggtt ccctccaaa acgtccaca gtgcacttt caagaggtc gtctaatgc gttgctcct actgtaaac aaaaaggag caacggttg gttgttgtt tcaagaact gttgcgaga tttatcact tcatttgcg cgagaactg cgaaatgtc attttatgc atgacaaga ctgtatttt gttgtagcc acgaagtac cgaagacta gtagtgtta tagtcacac ctcatgagt ttaatattc gtagatctg tttttgtgg aattggctg attacaaaa aggagcaat attcacaaa ttgaaaaag ttaacgtag tggttgggt cactctgtg ttacaaatc ttggaaaaa taaatccag ttatgtctg acactgttg aggtgatca ttccggtcg gttataatt gttctaggc tcttttggt caactgtcg agccttgga aattagttt cgtataaaa tatctactt cgattttaa atgctcatg ttaataatc accacgttg ataagagtt tcaccgttg atcattccc ttccaaagt cagtggttg aacgtaaat tgaaggaaa tcctccttt ggcacaagg atatagata agaaaaagc tggatgtta ttgagtttg acgatgagg aggaacctc aaaacgggg cagatcttt tgtcacccg aaatcacag atgcaattt ttgaggaac tcccccacg agctgcttg atggtattt cggtgcaag acatttttc cgaaactca tgtcttacg aggatttta ttagtatgg attcaaaat cggaaacga ccaactccg acaataacg gtaatactg aattgaaga aatcagacc aggaagttg aaaatgtgg aagagtttg acgataagg agtttaaat cgaacaaga acccacaaa aaacgtgta gtctgactc tcagttgca aactggata acagctttt ctccagtca ttaagaaac ggaaaataa ctccgcgtg aaaataact tattttttc aattgctgc aagtcgatg gtaactttg atgacagct cgagtgatt gtgggtcac cagtagggg ttaatagtg taaacatat gggactcgg atgtgtctg aagaaccgg tcacgtgtg aacgttgcc aatgtactt accggacac aagcgagct acaaacgac acttggacg catgcgaag ttatttgta caagcgaaa gcgattcgg aattgaatc aagatcaag atgcgaatc ttgaaacaa ctcaaattc acatcaacg aaacaacag tcaacaccg aacttccac aacctgttc tcatcacct catacgaat cttatcaaa acgaaattc atacctctg ttttaaata ctcaaactt tcgtagcgt cgaaatcaa atgcagaag tgatcactg ttcctcaaa atccggaga taattattc cgcagaccg aacaaccaa taaccgaac cgattgtta gtagtgaag tgttcacag tatcgcaat cgactgtgt cagtaaatg aacaaacct cgcaaccgt tctgtctaa acccgactg tctctcttt gtgttaatc cacctctaa cgttacaac caaacgcaa ctatttcgg ttaatccga cacagtcgg ttaatccga ttataaacc aaactatcg ctattaacc aagtgactc aaccagcga ctgtacaac cgcatcagt tggagtttc aacagccag gagttacaa tacaatcca tgccgtact ccactaatg tagctcctc tcattaatg taacacccc agaatcaga ttaccctca ccgataagt tattgaata attcaagta ttataaatt cgatgatta atgtacctg gaaatcgat gtgttatac cgtcgaaaa gcccaacaa aggttttta aaggaagac gatcgattg ctgccaaaa gaggtcacg aggaggaaa ccattgtga taccagcaa gttcgactc cagtgattg ttcaacact tgccatcta gtaatttta ttccgactt ttgttgaca ccttccagc aacaatcag gacaaaatg tacaatacg ttacaacga ttgcacctc aaatcaact caacgatca acccacaac cgctgatgc agttacagg ctgacagta atttgataa gtttgttgc ctggcattc aaccgacga tgatcattc aacagccac gagttttgg aaaatcaat tgattgtgg acgggaatg gttcattga gttggacac caccacaag tacaaccgg tctattacg gttttgaga cgatagtac agaacactg taatgcagt ctcaacagt ttttaccaa caacagtac caggagtct taactgcta acagcagtt attctacaa cgacacaag tattccaaa ctagtaaat tagaaccag tattagata tggggacga atagctttg tccttctaa atacaggac aattggtca attcgcagt cactcgaag tacctcaga attcactaa cgaatgttt tacaacaaa cagaaatca atcagtgta aatactcaa caccttcat ttcaaaaac cgaatagtc aaccaatta gtgtcacta atacaaaca gtataacac tacctacgg caccctttg tcccagaac aagggatac caactaata ttgttacgc caacaccta aaccggtac caaccactc agagtcggc caatgagtc gcgtcctac ctatgcaga cgaaagaac caaacaaag ttaaaacac caacagaaa aagttgaag caattagaa aagaaatta aaatcctgg agaaggccg tagaaccag tagtcgatt ttgataaaa agaacccta ttgagagta ccgacaaaa aactcatcg aaactcaat tagaaaatg atataaatg aagccaaga aagactctc ctttgaaac tcgtattcc aaaaacaga cccaagacg gttcgtaca aagtcagta acaacgtcg aggaacctc ttatacctg caatgacta accaaatca gccccaaag tagccagca acttcaaat ccactgaac cctcgtata aactaaaca acaatttca acaatcgca actctccag tacaaatag cacctttaa aaccgatca agtcgaact tcaaccccc caaaaccac tccacattc tgtcactga gtgacaaac aaaaccggc ctgaaaaaa tcgagaaga ggcttgcca tcgaacctc caaagcact tcccgctcg agaaaaagg agaatgaga acgtcccgt ccatacttt acacaattg agacacagg atggcttcc ggtactcat caacgtcca ttgcggact tgtggacca aagttttcg aagctgtgc aaaacgcac gggcggctc acaacatgc cgcttttgc cctcagact cgacaaata ttgtaaaca gtttgcaaa ttctcggct tgaagactt ccggactta agtatctca ttgagcaat tgccaggtg ccaccaagt gctcgaagt ataggccaa tgttcaatt tcccatcaa agtgtatag aaatcgagg acgagcttg tcgggcata caaacggaa gtgcgaggt gtgcgccac acatcaaaa gaaatgaac cctacgata tgttcgggt ggttagcgt cgaaacacc ggaaacctg aacatagtc tattctgtt cagatttag tgccaagaa ggggctctg taaataatt taccaatgg ccatgcgat tccgacaat tgaaactta ctagtaaat attcagtag gagtatacc gttcaaaaa tccaccgac gaggcttat tctgtttga gagattttg aagcaggag aaatggtta tagaatatt caggagaag ttattagat cagttttga ctgataaaa gagaaaagt attataatt cgaaaggaa ttggttgtt acatgtttc gaattgacg ataacttgg ttgttgatg caaccatga ccggaaatg cggccaggt ttataaatc attcttgtg atccgaatt gttattcga aagtcgttg aaattttgg gtcacaagc acatcatca tttttgctt taagaagaa tcatttgtg gagaagaat taacatacg attacaaat ttcccatcg aggaagata aaattccat gcacttgtg gaacacgac gttgtagga aatttctca actgatatt taatttgaa tttctgttt gtcagtatt taaacctta ctttattcg agtgagctc aaacaaatt gaacaaagg aaatgattt tttatactt ttaatttta tgtatgtaa tgtatattt tttttgtaa ttatggttg tatataatt gaaacttat gtaatctac ttccacggt agcacctat ttgccattt taaagttat tacattctt aaagtaata ttgtaagtt tcgtcagta tttttttca aatttatgt atatatttg gctgtgaga ttttatgca tttgtgcca ttggctgca taggtgcta tatatagtt ataaatttt gataaaagt gaatatgtg atatgtttc atagtccag tgtaattat ctagttata caagcgaaa aattacttt tatttagag taataatac tggtatcaa gacaaaaca acgtgtcgt gttgtggaa atacaattc agaatcttt tacgtacat tattattta ttgttttac tatgaatta aacggccgt gcatcaaat gcaaccaac agttcgaat tatattgaa tcatttata aaatgagca gattcccac gattgatat ttgtatact tcactgtat atctaacat cacccgaca ggcttcaaa ttttaattg aaatttctc tattttatg taagatttt tgtatattt acgaatgta ttttgtata tattttttt atgaacgat tgttttatt tatagggta cctattggc ataaacgac ttctaaact gcaaatctc actcaaatt caaacatct taaatctca aatgtgtgt gtgtataca ataacgaac atgttagaa ttgtgattg taattttac aaatgacaa tatcaacat atcagttaa ttagtagaa ctatggaaa gttctacag cgtgataag aggccttgt agtgttgta tataaattg aacgatttt acagtgtct taaattttt attgtatat aaagatcgt gctccttct ggtgctttt acttatact aattcgagc agctgtgct atacacttg taatatttc caata
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
