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Gene information
General information
TC004761 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6599558..6605864
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004761
    iBeetle screen iB_03837

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    vertex triplet vertex triplet anterior not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    vertex triplet vertex triplet posterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004761 RA
      MCSNETPAN TSPRLPMPF ALEGGPRAL LPIDQYRLQ 
      LYQYAVAER LRYPLLNPF PTPLTCYPL FPRALQPEE 
      PKPQHSYIG LIAMAILSS PEGKLVLSD IYQHILDHY 
      PYFRTRGPG WRNSIRHNL SLNDCFIKA GRSANGKGH 
      YWAIHPANV DDFRKGDFR RRKAQRKVR KHMGLAVDE 
      DGADSPSPP PLSVSPPVV PGPSTSVYH TVPARGPSR 
      KRQFDVASL LAPDSGEDT NEEDIDVVS SDQHQETSP 
      KQWPNMFPI VNYYQALLQ ARPGTTAET ATDTTDS
    CDS sequence
    • >TC004761 RA
      atgtgcagt aacgagact ccggccaac acctcaccg 
      aggctgccg atgcccttc gcgctcgaa ggtggcccc 
      cgggccctg ctgcccatc gaccagtac cggctccag 
      ctctaccag tatgccgtc gcagaaagg cttcgctac 
      cctctgctg aaccctttt ccgacgccg ctcacctgc 
      tacccgctc ttccccagg gcgctacag ccggaggag 
      cccaagccc cagcacagc tacatcggc ctcatcgcc 
      atggctatt ctaagctct cccgaagga aaactggtc 
      ctttcggac atctatcag cacatcctc gaccactat 
      ccttacttc aggacccgg ggaccgggt tggagaaac 
      tccatcagg cataatttg tctctcaat gattgtttc 
      atcaaggcg ggaagaagc gccaacgga aagggacat 
      tactgggca attcatccc gcaaatgtg gacgacttt 
      agaaaaggg gacttcagg aggaggaag gcacaaaga 
      aaggtgagg aagcacatg gggcttgcc gtcgatgaa 
      gatggggct gattcgcca agtccgccg cccttgtct 
      gtgagtccg cctgtcgtg ccagggcct tccacgtcc 
      gtttatcac acagtgccg gctcgaggt ccgtctcgc 
      aagcggcag ttcgacgtg gcgtcgctt ttggcgccg 
      gattccggt gaagacacc aacgaagag gacatcgac 
      gtcgtctcc agtgaccaa caccaagag acttcaccc 
      aaacagtgg cctaatatg tttcccatc gttaattat 
      taccaagca ttgttacaa gcgaggcca ggaacgact 
      gcagaaact gccaccgac accacagac tcttaa
    mRNA sequence
    • >TC004761 RA
      gctgttatg cccagatgg gtctctcac tagtatagt 
      ttattcagt cggtgttgc cggtacata attaataat 
      ctgactcaa actgatagg aacccatag ccagtttgt 
      gtcgatgtg gtgcaatgt gcagtaacg agactccgg 
      ccaacacct caccgaggc tgccgatgc ccttcgcgc 
      tcgaaggtg gcccccggg ccctgctgc ccatcgacc 
      agtaccggc tccagctct accagtatg ccgtcgcag 
      aaaggcttc gctaccctc tgctgaacc cttttccga 
      cgccgctca cctgctacc cgctcttcc ccagggcgc 
      tacagccgg aggagccca agccccagc acagctaca 
      tcggcctca tcgccatgg ctattctaa gctctcccg 
      aaggaaaac tggtccttt cggacatct atcagcaca 
      tcctcgacc actatcctt acttcagga cccggggac 
      cgggttgga gaaactcca tcaggcata atttgtctc 
      tcaatgatt gtttcatca aggcgggaa gaagcgcca 
      acggaaagg gacattact gggcaattc atcccgcaa 
      atgtggacg actttagaa aaggggact tcaggagga 
      ggaaggcac aaagaaagg tgaggaagc acatggggc 
      ttgccgtcg atgaagatg gggctgatt cgccaagtc 
      cgccgccct tgtctgtga gtccgcctg tcgtgccag 
      ggccttcca cgtccgttt atcacacag tgccggctc 
      gaggtccgt ctcgcaagc ggcagttcg acgtggcgt 
      cgcttttgg cgccggatt ccggtgaag acaccaacg 
      aagaggaca tcgacgtcg tctccagtg accaacacc 
      aagagactt cacccaaac agtggccta atatgtttc 
      ccatcgtta attattacc aagcattgt tacaagcga 
      ggccaggaa cgactgcag aaactgcca ccgacacca 
      cagactctt aaagtgatt tttagtgaa ttgtgtttt 
      caaatctac ttgaagact gtccgttat ttacaattt 
      tatttatac gaatagtca ctgttcata ttagatata 
      tttattgtt ctgttacaa acaatttca atcaacgtt 
      ttaatattt tcttaacaa taccggaat ttgtatata 
      aaattaaat gtgtatgag aact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY