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Gene information
General information
TC004758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6554378..6558812
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004758
    iBeetle screen iB_03835

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages slightly shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax complete fragmented - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax mostly fragmented - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax fragmented - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: The fragmented thorax and abdomen look sometimes like they are inside-outed.
    abdomen complete fragmented - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen fragmented - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: The fragmented thorax and abdomen look sometimes like they are inside-outed.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 9

    maxillary palp - Number of animals showing the phenotype: 1

    antenna shape irregular - Number of animals showing the phenotype: 9

    elytra size shortened - Number of animals showing the phenotype: 9

    legs orientation irregular - Number of animals showing the phenotype: 5
    legs shortened - Number of animals showing the phenotype: 5
    female genital lobes structure rounded - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004758 RA
      MQAKKRYLL LFVSCAFLA YWYFGGYRL KSSNMIVDD 
      HLPVFVNLD QVYTGSLQR QREISPISN NKLCSMENC 
      FDFGRCQQG FRVYVYPPD DNSTPSPSY QKLLNVLME 
      SRYYTADPR LACLFVLSI DTLDRDRLS ADYVRNMQS 
      RLQHLPHWN NGLNHVIFN LYSGTWPNY TENNLDFDY 
      GMAILAKAS MSDSHMRPG FDISIPLFH KVHPEKGGE 
      VGSVLANSL PLQKNYLLA FKGKRYVHG IGSDTRNSL 
      YHLHNRKDM IMVTTCRHG KSWKDMKDE RCDQDNKEY 
      DKYDYEVLL QNSTFCLVP RGRRLGSFR FLEALQAGC 
      IPVLLSNGW ALPFAQKID WSKAAIWAD ERLLLQVPY 
      IVRSLAPAK ILQLRQQTQ VLWDRYFSS IEKIVYTTL 
      EIIRERLPY EPFRDATVW NTLPGALVT LPSFSDHPK 
      SFPFQIEYS DVDGFTAII YCQLGLPLT PSAPLYRLV 
      TNIARSKYV SKIIVVWSN DKRPPSKTR WPLLPHNIS 
      LHVIQPEGE PTKPSISQR FYPHPQIDT AAVLSLDED 
      SVLTTDEVD FAYIVWKKF PDRIVGYPA RSHYWDDSK 
      STWGYTSKW TNDYSIVLT GAAFYHRYY NVLYTEWLS 
      PLLHKTVEQ SQNCEDILM NFLVSHVTR RPPIKVTQR 
      KQYKEQPTL GSWSPWNDP DHFIQRQTC LNTFAAVFG 
      YMPLLRSNL RLDPVLFKD TVANKRKKY RKIELVGS
    CDS sequence
    • >TC004758 RA
      atgcaagcg aaaaagcgc tatttactc ttattcgtg 
      tcctgtgcg ttcctagcg tactggtat ttcggcggc 
      taccgcttg aaaagcagc aacatgata gtggacgat 
      catttgcct gtgtttgtt aatttagat caagtttat 
      acgggaagt ctccaaagg cagcgagaa atttcgcca 
      atcagtaac aataagttg tgcagcatg gaaaactgt 
      tttgatttt ggtagatgt cagcaaggt tttcgcgtg 
      tatgtgtat cctcctgac gataatagt acgcctagt 
      ccgtcctac cagaaactg ctcaatgtt ttaatggaa 
      tcgcgatat tacactgcc gaccctagg ctagcttgc 
      ttattcgtt ctaagcata gacactctc gaccgagac 
      cgcctcagt gctgactac gtccgcaac atgcagtcg 
      cgtctgcag cacttgccg cattggaac aacggcctc 
      aaccacgta atcttcaac ctgtactca ggcacttgg 
      cctaactac accgaaaac aacctggac ttcgactac 
      ggcatggcc atcctggcc aaagccagc atgtccgac 
      agccacatg aggcccggc ttcgacatc agcatcccc 
      ctgttccac aaagtgcac cccgagaag gggggcgaa 
      gtgggctca gtcctcgcc aacagtttg cccctgcag 
      aagaactac ttactagct ttcaaaggc aaaagatac 
      gtgcatgga atcggctca gacacgaga aactccttg 
      taccacctc cacaacaga aaggacatg atcatggtc 
      accacgtgc cgccacggc aaaagctgg aaggacatg 
      aaggacgag cgctgcgac caagacaac aaagaatac 
      gacaagtac gactacgaa gtcttgctg caaaactcc 
      accttttgc ctggtcccc agagggcgg cggctgggg 
      tcgttccgg ttcctggaa gccctacaa gccggttgt 
      atcccagtt ttgttgtcc aatggctgg gctttgccc 
      ttcgcccaa aaaatagac tggagcaaa gccgccatt 
      tgggccgac gaaaggcta ctccttcaa gtaccttac 
      attgtacgc tcgttagca cccgcaaaa atcctacaa 
      ttgcgtcaa caaacgcaa gtcctgtgg gatcggtat 
      ttttcgtca attgagaag attgtctac acaacgtta 
      gaaatcata cgtgagcgt ttaccgtac gagccgttt 
      cgagatgct acggtttgg aatacgcta cctggggct 
      ttagtaact ttgccaagt ttctccgat cacccgaaa 
      agttttcca ttccagatt gaatatagt gatgttgat 
      ggtttcact gctattatt tactgccag ttaggactg 
      cccctaaca ccgtcagcc cctctgtac agacttgtc 
      accaacatc gctcgcagt aaatacgtc tcaaagata 
      atcgtggtg tggtcgaac gacaagcgt ccccccagc 
      aagacgcgg tggccgttg ttgccccac aacatcagt 
      ctgcacgtg atccagcct gagggcgag cccacgaag 
      cccagcatt tcgcagcgc ttctacccg cacccgcaa 
      atcgacacg gcagccgtg ctatcccta gacgaagac 
      tccgttctg acaacagac gaagtcgat ttcgcgtac 
      atcgtgtgg aagaaattc cccgaccgt atcgtggga 
      taccctgca cggtcccac tactgggac gactccaag 
      tccacgtgg ggctacacc tcgaagtgg acgaacgac 
      tactcgatt gtcctgaca ggagcagcg ttctaccac 
      cgatattac aacgttcta tacaccgag tggctgagc 
      ccgctcttg cacaaaacg gtggagcag agccagaac 
      tgcgaggac attctaatg aatttcctg gtcagtcat 
      gtgacacgg cggccgccc atcaaagtg acccagcgg 
      aaacagtac aaagaacag ccgactctc gggagttgg 
      tcgccgtgg aatgacccc gaccatttt atccaaaga 
      cagacttgc ctgaacact tttgcggca gtgttcggc 
      tacatgccg ctgctgcga tccaatctg cggctagat 
      ccagtgctt tttaaagat acggtagcc aataaaagg 
      aaaaagtac agaaaaatc gaactggtg ggcagttag
    mRNA sequence
    • >TC004758 RA
      cgaaaatac aaagcaatt gacagattt gataaaatt 
      tgtattttg tgaaaaatg tgatacgga cgaaagttg 
      ttagttaaa atgcaagcg aaaaagcgc tatttactc 
      ttattcgtg tcctgtgcg ttcctagcg tactggtat 
      ttcggcggc taccgcttg aaaagcagc aacatgata 
      gtggacgat catttgcct gtgtttgtt aatttagat 
      caagtttat acgggaagt ctccaaagg cagcgagaa 
      atttcgcca atcagtaac aataagttg tgcagcatg 
      gaaaactgt tttgatttt ggtagatgt cagcaaggt 
      tttcgcgtg tatgtgtat cctcctgac gataatagt 
      acgcctagt ccgtcctac cagaaactg ctcaatgtt 
      ttaatggaa tcgcgatat tacactgcc gaccctagg 
      ctagcttgc ttattcgtt ctaagcata gacactctc 
      gaccgagac cgcctcagt gctgactac gtccgcaac 
      atgcagtcg cgtctgcag cacttgccg cattggaac 
      aacggcctc aaccacgta atcttcaac ctgtactca 
      ggcacttgg cctaactac accgaaaac aacctggac 
      ttcgactac ggcatggcc atcctggcc aaagccagc 
      atgtccgac agccacatg aggcccggc ttcgacatc 
      agcatcccc ctgttccac aaagtgcac cccgagaag 
      gggggcgaa gtgggctca gtcctcgcc aacagtttg 
      cccctgcag aagaactac ttactagct ttcaaaggc 
      aaaagatac gtgcatgga atcggctca gacacgaga 
      aactccttg taccacctc cacaacaga aaggacatg 
      atcatggtc accacgtgc cgccacggc aaaagctgg 
      aaggacatg aaggacgag cgctgcgac caagacaac 
      aaagaatac gacaagtac gactacgaa gtcttgctg 
      caaaactcc accttttgc ctggtcccc agagggcgg 
      cggctgggg tcgttccgg ttcctggaa gccctacaa 
      gccggttgt atcccagtt ttgttgtcc aatggctgg 
      gctttgccc ttcgcccaa aaaatagac tggagcaaa 
      gccgccatt tgggccgac gaaaggcta ctccttcaa 
      gtaccttac attgtacgc tcgttagca cccgcaaaa 
      atcctacaa ttgcgtcaa caaacgcaa gtcctgtgg 
      gatcggtat ttttcgtca attgagaag attgtctac 
      acaacgtta gaaatcata cgtgagcgt ttaccgtac 
      gagccgttt cgagatgct acggtttgg aatacgcta 
      cctggggct ttagtaact ttgccaagt ttctccgat 
      cacccgaaa agttttcca ttccagatt gaatatagt 
      gatgttgat ggtttcact gctattatt tactgccag 
      ttaggactg cccctaaca ccgtcagcc cctctgtac 
      agacttgtc accaacatc gctcgcagt aaatacgtc 
      tcaaagata atcgtggtg tggtcgaac gacaagcgt 
      ccccccagc aagacgcgg tggccgttg ttgccccac 
      aacatcagt ctgcacgtg atccagcct gagggcgag 
      cccacgaag cccagcatt tcgcagcgc ttctacccg 
      cacccgcaa atcgacacg gcagccgtg ctatcccta 
      gacgaagac tccgttctg acaacagac gaagtcgat 
      ttcgcgtac atcgtgtgg aagaaattc cccgaccgt 
      atcgtggga taccctgca cggtcccac tactgggac 
      gactccaag tccacgtgg ggctacacc tcgaagtgg 
      acgaacgac tactcgatt gtcctgaca ggagcagcg 
      ttctaccac cgatattac aacgttcta tacaccgag 
      tggctgagc ccgctcttg cacaaaacg gtggagcag 
      agccagaac tgcgaggac attctaatg aatttcctg 
      gtcagtcat gtgacacgg cggccgccc atcaaagtg 
      acccagcgg aaacagtac aaagaacag ccgactctc 
      gggagttgg tcgccgtgg aatgacccc gaccatttt 
      atccaaaga cagacttgc ctgaacact tttgcggca 
      gtgttcggc tacatgccg ctgctgcga tccaatctg 
      cggctagat ccagtgctt tttaaagat acggtagcc 
      aataaaagg aaaaagtac agaaaaatc gaactggtg 
      ggcagttag tccagggtt ttgttctaa tatttttaa 
      atcggttga tctctgtac tctgccggt gttgggaac 
      gtatacttt gtatccatg tttcatgtg caatgtttt 
      gtgttaact tgatcaaat ttcatgcat atccttgta 
      tcagctgac aacttaaac ttcttttat acttttaca 
      aataggttt tatataaaa aatgatatc ctaagtata 
      gagccatat actgtagtt ggaaccaaa tattgtttt 
      tattagttg atattttat aatcgagcg atttatttt 
      atacatcgt gactgtaaa attggatca gttacaaga 
      tttcgaatg ataaaatat tcccaataa aaaattaaa 
      tcgccg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY