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Gene information
General information
TC004747 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6082222..6091067
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004747
    iBeetle screen iB_03832
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004747 RA
      MFLFRIFFV VAFGFAYEW DTSVLQIRP WGVDNCQTD 
      DSDCQQQAE TEIAHCYDS CTDPENCVI KVAVILPDQ 
      EFYLVNLKK AEATIEEFK ADVGIKYNF EFKFYNDSC 
      MQEFATVHL IPASENCTR LILGPICDY CLASVSRVA 
      KYIGYHGMP VITPGGFTH DFVENKTGC SNEYYMTIN 
      TGPIDYRSF GEFFHLIMA RYKWKKIAL MYEKNQQNE 
      VGGENSCKF ILGSVVEEV KSVENLKYV DGDLVLLNK 
      DYSNYLKEN VGVSYGIIL TCTTVANLR EFAIAAQKL 
      NMMESGEYT VFNFQTYYN TTEQALKPW YDANDTNER 
      NEMARKGFR SIFTFTSSE AINFRNRRV EGGSIFLDG 
      LYDGMMLYT SALNQTLSA SENLTGYNM VKKMMGTTF 
      AGKHGNVTM NCNGQRVAT YVMLQLNGS DKYDVVAEF 
      TTLNKNITS WNVQWISGK DPLDTPVCG YDMSLCPKF 
      DAVKVLLIS LILVFCLAI LIVGIILYR HFKLKKAIY 
      SMAWKIDYD DIVFVPIKP RSSFQSTSS ARRGSQITF 
      LGTDGLSMA GDRQLYTEV GFYKAIRVA VKKIQDVKI 
      ELTREQLIE LKVMKDLSH DNLVKFHGA CLDIPNCIL 
      SEYCAKGSL QDILENDNV KIDTTFKMS LIMDIVRGM 
      NYLHNSDIK SHGALKSTN CLVDSRFVL KISDFGLHF 
      LRKHGPHND GTKDHSYWE RQLWTAPEL LREENPPPC 
      GTPKGDTYS FALIMHEII VRQGVFHLD EFKTAEEKV 
      DAVRWGPDA NGGQPLRPS LGEALCEEE VASLMKKCW 
      SEDAADRPD FNTLKTKLK HINKESDGN LLDNLLARM 
      EQYANNLES LVEERTADY LEEKRKCEE LLYQLLPKS 
      VAQQLITGE SVIAETFEN VTIHFSDIV GFTQLSADS 
      TPLQVVEFL NDLYTCFDS IIENFDVYK VETIGDSYM 
      VVSGLPVRN GNNHAREIA RMSLALLKA VKTFKIRHR 
      PDESLKLRI GIHTGPCVA GVVGLKMPR YCLFGDTVN 
      TASRMETNG SPLRVHISP ATKEFLDRF GTFEIERRG 
      EVELKGKGK MVTYWLNGE CEKKTTIKA PQTEPKPGK 
      LPSTPLPNS VRKQMANSG KALNEMNLQ DHQIDEAAL 
      PLLSITSPP EYNSNA
    CDS sequence
    • >TC004747 RA
      atgttctta tttaggata ttttttgtt gttgcgttt 
      ggttttgcc tatgaatgg gacacttcg gttctgcag 
      atccgtcca tggggggtg gacaactgc caaacagac 
      gactcagac tgtcaacag caagcggaa acggaaatt 
      gcgcattgt tacgattct tgcactgac cccgaaaat 
      tgtgtaatt aaggtagct gttatacta cccgaccag 
      gaattttat ttagtcaac ttgaagaag gccgaggca 
      acaattgag gagtttaag gccgacgta ggcataaaa 
      tacaatttc gaattcaag ttctacaac gattcctgc 
      atgcaggaa ttcgccacc gtgcactta attcctgcg 
      agtgagaat tgtactcgt ttaatactt ggaccaatc 
      tgtgactac tgtctcgca agtgtgtcc cgagttgca 
      aaatacatc ggttatcac ggaatgccc gtgataact 
      cccggaggc tttacgcac gattttgtg gaaaataag 
      acgggttgt tcgaatgaa tattatatg acgataaat 
      accggtcca attgactac agatctttc ggtgaattc 
      tttcactta ataatggcc aggtacaaa tggaaaaaa 
      atcgccctg atgtacgaa aaaaatcag caaaacgag 
      gtcggtggc gagaacagt tgcaagttt atattgggg 
      agcgtggtt gaggaggtg aaatcggtt gagaatctt 
      aaatacgtg gacggtgat ttggtttta ttaaataag 
      gattattcc aactacttg aaggaaaat gtgggggtc 
      agctacggc atcattttg acttgcacc actgtggcc 
      aatctccgc gagttcgcc atcgcggcc cagaaatta 
      aacatgatg gaaagcggg gagtacaca gttttcaat 
      ttccaaacg tattacaac acaaccgag caagcctta 
      aagccctgg tacgatgca aatgacact aatgaacgc 
      aacgaaatg gcgagaaaa ggttttcgc tcaattttc 
      acgtttaca tcgtctgaa gcaattaac tttcgcaat 
      cggcgagtg gagggcggc tcgattttt cttgatggc 
      ttgtacgac ggaatgatg ctgtacaca agtgcttta 
      aatcagacc ttatcagct tcggagaac ttaacgggc 
      tacaacatg gttaaaaaa atgatgggg accactttt 
      gcagggaag cacgggaac gtaaccatg aactgcaac 
      ggacagagg gttgcaacc tacgtaatg ctccagctg 
      aatggctcg gataagtat gatgtcgtt gctgaattt 
      acaacactt aataaaaat atcacttct tggaacgtg 
      cagtggatc agtgggaaa gatccactt gatacgcct 
      gtctgtggc tacgatatg tccctgtgc ccaaagttt 
      gatgcggtt aaagttctg ttaatttcg cttatacta 
      gtgttctgc ttggcgata ttgatcgtg gggataatt 
      ctgtatagg cactttaaa cttaagaaa gctatctat 
      tctatggcc tggaaaatt gattacgat gatatcgtt 
      ttcgtgccg atcaagccg aggagcagt tttcaatcg 
      acttcgtct gcccgccga ggaagccaa attacgttc 
      ttagggacg gacggctta agcatggct ggggatcgt 
      caactgtac acggaagta ggcttctac aaggcaatt 
      agagtggcg gtgaaaaaa attcaagac gtcaagatc 
      gagctaacc cgcgagcag ttgatcgag ttgaaagtg 
      atgaaagac ttatcgcat gataattta gtgaagttt 
      cacggcgct tgtttagac attcccaac tgcattttg 
      agtgaatat tgcgcgaaa ggcagtttg caggatata 
      ttagaaaat gataacgtg aaaatcgat accacgttt 
      aaaatgtcg ttaataatg gacattgtt cgcggcatg 
      aactacctg cataattcc gatattaaa tctcacggc 
      gctttaaaa tcaacaaac tgtttagtg gacagcagg 
      ttcgtgttg aaaatctcc gacttcgga ctgcatttt 
      ttacgaaag cacggacct cacaacgat ggaacgaag 
      gatcatagt tactgggag cgtcagctt tggacggcc 
      ccggagctc ctccgggag gagaacccc cctccttgc 
      gggactccg aaaggagac acttactca tttgccctg 
      ataatgcac gaaattatc gtacgacaa ggggttttc 
      catttagat gaattcaaa acagccgaa gaaaaagtc 
      gatgctgtt cgctgggga ccagacgca aacgggggc 
      caaccgctt cgcccctct ttgggggag gcgctgtgc 
      gaggaagag gtggcctcg ctgatgaag aagtgctgg 
      tcggaggac gccgccgac aggcccgac ttcaacacc 
      ctcaagacc aaactgaag cacataaac aaggaatcc 
      gatggaaat ctcctcgac aatctgctg gcgcggatg 
      gagcagtac gccaacaac ctggagtcg ctagtggag 
      gaacgcact gctgattac ttggaggag aagcgcaaa 
      tgcgaggag ttgctctat cagctgctg ccgaaaagc 
      gtcgcgcaa cagctgata acgggggag tcggtcatt 
      gccgaaacc ttcgaaaat gtcaccata cacttcagc 
      gacatcgtc ggcttcacc cagctgtcc gccgacagc 
      accccgctg caagttgtc gagttcttg aacgacctc 
      tacacatgc tttgattcc attatcgag aacttcgat 
      gtttacaaa gtggaaact atcggcgac tcgtacatg 
      gtcgtgtca ggactgccg gtcaggaat gggaacaac 
      cacgcgcga gaaatcgca cggatgtca ctagcgtta 
      ctaaaagcc gttaaaacc ttcaaaatc aggcaccgg 
      ccggacgag agcttgaaa cttcgaata gggatccac 
      accgggcct tgcgttgcc ggcgttgtc gggctaaaa 
      atgccaaga tactgtctc ttcggagac acagtcaac 
      acggcatcc agaatggag accaacgga agcccgtta 
      agggtgcac ataagccca gccacgaaa gaattcctg 
      gatcggttc gggacgttt gaaatcgaa cggagaggc 
      gaggttgag ttgaaggga aagggcaag atggtgacg 
      tactggctt aacggcgaa tgcgagaag aaaaccacg 
      atcaaggcg ccgcagacc gagcccaag cccgggaag 
      ctcccaagc accccctta cctaacagt gtcagaaaa 
      caaatggca aatagcggc aaggcttta aacgagatg 
      aatttacaa gatcatcaa attgacgaa gccgcgtta 
      ccgttgctt tccataacg tcaccccca gagtacaat 
      tcgaatgcg tga
    mRNA sequence
    • >TC004747 RA
      cctatctat atttgctat cgacaacag tattgaagt 
      tagtatatt cgttgcttc cataaaatg cgctgagtg 
      cgggtcgtt aattgtccg cggttatgt cgtaaattt 
      cgagtggtt agttatgtt cttatttag gatattttt 
      tgttgttgc gtttggttt tgcctatga atgggacac 
      ttcggttct gcagatccg tccatgggg ggtggacaa 
      ctgccaaac agacgactc agactgtca acagcaagc 
      ggaaacgga aattgcgca ttgttacga ttcttgcac 
      tgaccccga aaattgtgt aattaaggt agctgttat 
      actacccga ccaggaatt ttatttagt caacttgaa 
      gaaggccga ggcaacaat tgaggagtt taaggccga 
      cgtaggcat aaaatacaa tttcgaatt caagttcta 
      caacgattc ctgcatgca ggaattcgc caccgtgca 
      cttaattcc tgcgagtga gaattgtac tcgtttaat 
      acttggacc aatctgtga ctactgtct cgcaagtgt 
      gtcccgagt tgcaaaata catcggtta tcacggaat 
      gcccgtgat aactcccgg aggctttac gcacgattt 
      tgtggaaaa taagacggg ttgttcgaa tgaatatta 
      tatgacgat aaataccgg tccaattga ctacagatc 
      tttcggtga attctttca cttaataat ggccaggta 
      caaatggaa aaaaatcgc cctgatgta cgaaaaaaa 
      tcagcaaaa cgaggtcgg tggcgagaa cagttgcaa 
      gtttatatt ggggagcgt ggttgagga ggtgaaatc 
      ggttgagaa tcttaaata cgtggacgg tgatttggt 
      tttattaaa taaggatta ttccaacta cttgaagga 
      aaatgtggg ggtcagcta cggcatcat tttgacttg 
      caccactgt ggccaatct ccgcgagtt cgccatcgc 
      ggcccagaa attaaacat gatggaaag cggggagta 
      cacagtttt caatttcca aacgtatta caacacaac 
      cgagcaagc cttaaagcc ctggtacga tgcaaatga 
      cactaatga acgcaacga aatggcgag aaaaggttt 
      tcgctcaat tttcacgtt tacatcgtc tgaagcaat 
      taactttcg caatcggcg agtggaggg cggctcgat 
      ttttcttga tggcttgta cgacggaat gatgctgta 
      cacaagtgc tttaaatca gaccttatc agcttcgga 
      gaacttaac gggctacaa catggttaa aaaaatgat 
      ggggaccac ttttgcagg gaagcacgg gaacgtaac 
      catgaactg caacggaca gagggttgc aacctacgt 
      aatgctcca gctgaatgg ctcggataa gtatgatgt 
      cgttgctga atttacaac acttaataa aaatatcac 
      ttcttggaa cgtgcagtg gatcagtgg gaaagatcc 
      acttgatac gcctgtctg tggctacga tatgtccct 
      gtgcccaaa gtttgatgc ggttaaagt tctgttaat 
      ttcgcttat actagtgtt ctgcttggc gatattgat 
      cgtggggat aattctgta taggcactt taaacttaa 
      gaaagctat ctattctat ggcctggaa aattgatta 
      cgatgatat cgttttcgt gccgatcaa gccgaggag 
      cagttttca atcgacttc gtctgcccg ccgaggaag 
      ccaaattac gttcttagg gacggacgg cttaagcat 
      ggctgggga tcgtcaact gtacacgga agtaggctt 
      ctacaaggc aattagagt ggcggtgaa aaaaattca 
      agacgtcaa gatcgagct aacccgcga gcagttgat 
      cgagttgaa agtgatgaa agacttatc gcatgataa 
      tttagtgaa gtttcacgg cgcttgttt agacattcc 
      caactgcat tttgagtga atattgcgc gaaaggcag 
      tttgcagga tatattaga aaatgataa cgtgaaaat 
      cgataccac gtttaaaat gtcgttaat aatggacat 
      tgttcgcgg catgaacta cctgcataa ttccgatat 
      taaatctca cggcgcttt aaaatcaac aaactgttt 
      agtggacag caggttcgt gttgaaaat ctccgactt 
      cggactgca ttttttacg aaagcacgg acctcacaa 
      cgatggaac gaaggatca tagttactg ggagcgtca 
      gctttggac ggccccgga gctcctccg ggaggagaa 
      cccccctcc ttgcgggac tccgaaagg agacactta 
      ctcatttgc cctgataat gcacgaaat tatcgtacg 
      acaaggggt tttccattt agatgaatt caaaacagc 
      cgaagaaaa agtcgatgc tgttcgctg gggaccaga 
      cgcaaacgg gggccaacc gcttcgccc ctctttggg 
      ggaggcgct gtgcgagga agaggtggc ctcgctgat 
      gaagaagtg ctggtcgga ggacgccgc cgacaggcc 
      cgacttcaa caccctcaa gaccaaact gaagcacat 
      aaacaagga atccgatgg aaatctcct cgacaatct 
      gctggcgcg gatggagca gtacgccaa caacctgga 
      gtcgctagt ggaggaacg cactgctga ttacttgga 
      ggagaagcg caaatgcga ggagttgct ctatcagct 
      gctgccgaa aagcgtcgc gcaacagct gataacggg 
      ggagtcggt cattgccga aaccttcga aaatgtcac 
      catacactt cagcgacat cgtcggctt cacccagct 
      gtccgccga cagcacccc gctgcaagt tgtcgagtt 
      cttgaacga cctctacac atgctttga ttccattat 
      cgagaactt cgatgttta caaagtgga aactatcgg 
      cgactcgta catggtcgt gtcaggact gccggtcag 
      gaatgggaa caaccacgc gcgagaaat cgcacggat 
      gtcactagc gttactaaa agccgttaa aaccttcaa 
      aatcaggca ccggccgga cgagagctt gaaacttcg 
      aatagggat ccacaccgg gccttgcgt tgccggcgt 
      tgtcgggct aaaaatgcc aagatactg tctcttcgg 
      agacacagt caacacggc atccagaat ggagaccaa 
      cggaagccc gttaagggt gcacataag cccagccac 
      gaaagaatt cctggatcg gttcgggac gtttgaaat 
      cgaacggag aggcgaggt tgagttgaa gggaaaggg 
      caagatggt gacgtactg gcttaacgg cgaatgcga 
      gaagaaaac cacgatcaa ggcgccgca gaccgagcc 
      caagcccgg gaagctccc aagcacccc cttacctaa 
      cagtgtcag aaaacaaat ggcaaatag cggcaaggc 
      tttaaacga gatgaattt acaagatca tcaaattga 
      cgaagccgc gttaccgtt gctttccat aacgtcacc 
      cccagagta caattcgaa tgcgtgaac aatcctcct 
      gctaaatta aggtgaagt ggatcattt tattgtata 
      cggcaggat tattaaaca gtattgaac caacaacct 
      aagtcaaat taggttcag atttgtgat tcagtttcc 
      tctattttc atatatgtt atttatggt tattttaat 
      tattgttct aaataaaga tttttaaac tga