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Gene information
General information
TC004745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 6057932..6068535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004745
    iBeetle screen iB_03831
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature specific subset lateral not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature ventral mostlynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    leg musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva bristle irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva dorsalcuticularisation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus sometimes duplicated - Penetrance (percentage of animals/eggs on slide): more than 80%
    mesothoracic tracheal opening not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment anteroposterior-axis decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen tracheal opening not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004745 RA
      MASTAASES PGRHESDLY TRPGWVDAA VALDQLDQG 
      KASGDRGIL WVRGRIQRQ LRSLGRILD THAGKFLFV 
      SVLAIATFS VGLKSMAFH SDIELLWAE PSTGAEATP 
      QEMLSTHQM IVQTAVEPD IDLLHPHGL LEHLALLQK 
      ATQVTITMF DITWRLKDF CQAPSIPNF DAHFIEQIF 
      ENMMPCSII TALDCFWEG SKLLGPDYP VHIPYGIGK 
      HIKWTNLNP SQLVSELKQ KESQFDYHS LEDYFKRAG 
      ITSGYQEKP CLNPRDPEC PVTAPNYNS SQNLDIGAE 
      LTSGCYGFA AKYMHWPED LIVGGANKN KSGHIKQAK 
      ALQTVVQLM GEHELFEFW SGHYKVHHI GWNQEKATT 
      VLNAWQKKF AQEVDRLTK SKKSSNYIF VTFSTANLN 
      KMLKEASKT DVVKLGVVL GVAAVYGWV AQSGLAALG 
      VLVLASSAA AALGVCSLI GLPINLLST HVLPFVTVG 
      LAMREMFLI LSTHMRNLS PPEVLQRTG PSIISAALV 
      NSAAFLAAA VLPVPALRV FCLQCAVLV IFHGAALLI 
      VFPSLLALE QRCRKTDVP CFRNETKSK PPTNNNNDP 
      EHGENLLTA EVICQQKKS LLTWLVNRY FTSVLVKPF 
      VKVVLCLTY AVLIIFCVF NGLKLEFDV RLSTFLPKN 
      TQEYKYLEA QNKFFGFYN FYLVTTELE YPLSQPLLY 
      EYHSSLTNV PHVLKDSNG GLNMNDFWL ANFRDFLLD 
      LQQEFDLNR KNNFLNSEK WFPNATEKA VLAFKLLAQ 
      TGIVEFPVD KSQIFKKQL VDKGIIDPK AFYNYLSVW 
      NSNDQMSYS SSQANLTPK PCQHYSSKA EFDLKIPKS 
      RPLVYTQMP FYLKNLKST GKIIETLKQ IKQISEEYN 
      QRGLKNYPI GLIFAYFNQ FLLLDRLLA AQLVLTLLA 
      AVLVACVLV QWTKLWCSV FSIIICMLL MCLVNVNAL 
      SGTIGALHF IIVSRNIFL ILTGFLSAL GNCERRLKM 
      SLELYAEPI LKGDFGLFV CVSVLVTSQ FEFIQCHFF 
      TALLVSLCS STINSLVFF PILLLLAGP RAELYQLEH 
      TDRIATPTP PPPPPPPIR TPTYSKPSR RNTSVKATR 
      EPSLTTITE ESCNQSIIV EPQVTVEYS SPESSSSGP 
      YTTKVTATA NIKVELVAP VYRPKCSKC HRSKCKRRQ 
      QCRCCKDSD SSDSSAEAE PCPSS
    CDS sequence
    • >TC004745 RA
      atggcctcc acggcggcg tcggagtcg ccggggcgc 
      cacgagtcg gacctgtac accaggccg ggatgggtc 
      gacgccgcc gtcgcgctg gaccagctc gaccagggc 
      aaagcgagc ggagaccgc ggcattctg tgggttcgt 
      ggccgaatt cagcgccag ctgcgctcc ctgggccgc 
      atcctcgat acgcacgcc ggcaagttc ctcttcgtg 
      agcgtgctg gcgatcgcc accttctcc gtgggcctg 
      aagagcatg gccttccac tcggacatc gagctgctg 
      tgggccgag ccctccacg ggcgccgag gcgacgccg 
      caggagatg ctctccacg caccagatg atcgtgcag 
      acggccgtc gagcccgac atcgacctc ctgcatccg 
      cacggcctc ctggagcac ctcgcgctc ctccagaag 
      gccacgcag gtcaccatc acgatgttc gacatcacc 
      tggcgcctc aaggacttc tgccaagca cccagcatt 
      cccaatttt gatgcgcac tttattgaa cagattttc 
      gaaaatatg atgccctgc tccatcatc acggcgctc 
      gactgcttc tgggaaggc tccaaattg ctcggacct 
      gattatcct gtccatata ccatatgga ataggcaag 
      catataaaa tggacaaac ctcaacccc agtcagtta 
      gtatccgag ttaaagcaa aaagaatcc cagtttgat 
      tatcattct ttagaggat tatttcaag cgtgctgga 
      ataacctct ggatatcag gaaaagccg tgtttgaac 
      ccccgcgac ccggaatgt ccagtgacg gctccaaat 
      tacaattcg agtcaaaat ttagatatt ggtgctgaa 
      ctaaccagt ggttgttac ggtttcgcc gcgaaatac 
      atgcactgg ccggaagac ttgatcgtt ggcggtgcc 
      aataagaat aaatctggt catattaaa caagccaaa 
      gctttacaa actgttgta caattaatg ggcgaacac 
      gaactgttc gaattctgg tctggacat tacaaagtg 
      caccacatc ggatggaac caggagaag gccacaacc 
      gtactgaac gcctggcag aagaagttc gcacaggag 
      gtggatcgt ctgacgaag agcaagaag tcgtcgaat 
      tacatcttc gtgacgttc tccaccgcc aatttgaac 
      aagatgttg aaggaggcg tcgaagacg gacgtggtg 
      aagctgggg gtggtgctg ggggtggcg gcggtgtac 
      gggtgggtg gcccagtcg gggctggct gccttggga 
      gtgctggtc ttggcgagc tcagccgcc gccgcgctg 
      ggcgtttgc agtttgatc ggactgccg ataaaccta 
      ctgagcacg catgtgtta ccttttgtt actgttgga 
      ctcgcaatg agagaaatg ttccttatt ttgtcgacg 
      catatgagg aatctgtcg ccaccggaa gtgctacaa 
      aggacgggt cctagcatt atctcggcg gcgttagtg 
      aattccgcc gcgttctta gcggctgct gtactgcca 
      gtgccggcg ttacgtgtt ttctgttta caatgtgcg 
      gtacttgtt atattccac ggtgctgca ttgcttatt 
      gtgttccct tcgctcttg gccctcgag cagcgctgt 
      cgaaagact gacgtgccg tgcttccgg aacgagacc 
      aagtccaag cctcccaca aacaacaac aatgacccg 
      gaacatggt gaaaacttg ttgactgcg gaggtgatc 
      tgtcagcaa aagaaaagt cttttaacg tggttagtg 
      aatcgttac ttcacatca gtgcttgtt aaaccattt 
      gtgaaggtg gttttgtgt ttgacgtac gctgtgttg 
      ataatattt tgcgttttt aacggattg aagttggag 
      tttgacgtg agattatcg acattttta ccaaagaat 
      acgcaggag tacaaatat ttagaagct caaaataaa 
      ttctttggt ttttataat ttttattta gtgacgacc 
      gagttggag tacccttta agccaaccg cttctgtat 
      gaataccac tcgtcttta acaaatgta cctcatgtt 
      ttgaaagac tcgaatggg ggcttgaat atgaacgat 
      ttttggtta gccaatttc cgggatttt ttactagat 
      ttacagcaa gagtttgac ttgaatcgg aaaaacaat 
      ttccttaat agcgagaag tggtttccg aatgcgacc 
      gaaaaggca gtattagcg ttcaaattg ttagcgcag 
      actggcatt gtggagttt ccagtcgat aaaagtcaa 
      atttttaag aaacaactt gtcgataaa ggcattata 
      gatcccaaa gcgttttat aactattta tcagtttgg 
      aatagtaac gatcaaatg tcatattca agctcccag 
      gcgaatcta acaccgaag ccttgtcaa cactatagt 
      tctaaggcc gagttcgat ttaaaaatt ccaaagtca 
      cgacctctt gtttatact caaatgcca ttctacctc 
      aagaatttg aaaagtact ggaaaaatc atagaaact 
      ctcaaacag attaaacaa atttccgaa gagtacaat 
      caaagggga ttaaagaat tatcccatt ggacttata 
      ttcgcttat tttaatcag ttcctacta ctagaccgc 
      ttgttggct gcccagctg gtactcacg ttactcgcg 
      gccgttcta gtagcgtgt gttcttgta cagtggaca 
      aaactttgg tgttccgta ttcagcatt ataatctgt 
      atgttgttg atgtgtttg gtgaacgta aatgcttta 
      agtggaaca ataggtgca ttgcatttt ataatagtg 
      agtcgcaat atcttcctt atcctaaca ggtttctta 
      agcgcttta ggaaattgt gaacggaga ctcaaaatg 
      tcgttagaa ttatacgcc gaacctata ctcaaaggc 
      gattttgga ctatttgta tgtgtctca gtattagta 
      acttcccag ttcgagttc atccaatgc cattttttc 
      accgcattg ctggtatcc ctgtgcagt tcaactatc 
      aattctctg gttttcttc cccattttg ctactcctc 
      gccggtcct cgagccgaa ctctaccaa ctagaacac 
      accgaccgg attgccaca ccaacacca cctccacct 
      cctcctccc cctatccga accccaacc tactcaaag 
      ccctcccgc cgaaacact tccgtcaaa gccactcga 
      gagcccagc ctcaccact attacagag gagagttgc 
      aatcagagt attatagtc gagccacaa gtcactgta 
      gaatacagt agtccagaa tccagttct agtggccct 
      tacacaacc aaagtaacc gccactgct aatattaag 
      gtagagtta gttgcccct gtatataga cctaaatgt 
      agtaagtgt cacagatcg aagtgtaag aggcgacag 
      cagtgtcgc tgttgcaag gactcagac agtagcgat 
      agtagtgcc gaggcagaa ccgtgcccc agtagctag
    mRNA sequence
    • >TC004745 RA
      ggtgctatt agccggatt ccactgtac ttacgtttc 
      aattgcaca gagaatcgg gttggggct tatcggcgg 
      tggtggatg tgtggagtc tattagact gtctgtata 
      tcgtatgat ggtgagaaa aaaaacaaa agtaatgta 
      atttggaga atttacatt ctaaacata tactgtggg 
      tggtcttcg ttcggagaa actcgacgc cggtcgcct 
      gtagagagc gacccaagg cattgcgat tacaatgaa 
      cgggcaaca gcttagcag tcggctcgt cagtcgaac 
      tcgcagttg tgttggttg tggagtcgt gagtcgcac 
      tgcgtgtca ctttgttgt tgttcccca cgttaaatg 
      tgttttggt cttggccgt ttgtgcgtc cgagtgcga 
      gtggcggaa gtgtcgaaa gcagcgtgt ccgaggatg 
      cccggtgca cgcgccagg actataggg cccgagccc 
      gcgccggag gccagtgcc gtggtctgt ggaatgatg 
      cgcggtttg gagcgggcc cgcgcccgc taagaggat 
      ggcctccac ggcggcgtc ggagtcgcc ggggcgcca 
      cgagtcgga cctgtacac caggccggg atgggtcga 
      cgccgccgt cgcgctgga ccagctcga ccagggcaa 
      agcgagcgg agaccgcgg cattctgtg ggttcgtgg 
      ccgaattca gcgccagct gcgctccct gggccgcat 
      cctcgatac gcacgccgg caagttcct cttcgtgag 
      cgtgctggc gatcgccac cttctccgt gggcctgaa 
      gagcatggc cttccactc ggacatcga gctgctgtg 
      ggccgagcc ctccacggg cgccgaggc gacgccgca 
      ggagatgct ctccacgca ccagatgat cgtgcagac 
      ggccgtcga gcccgacat cgacctcct gcatccgca 
      cggcctcct ggagcacct cgcgctcct ccagaaggc 
      cacgcaggt caccatcac gatgttcga catcacctg 
      gcgcctcaa ggacttctg ccaagcacc cagcattcc 
      caattttga tgcgcactt tattgaaca gattttcga 
      aaatatgat gccctgctc catcatcac ggcgctcga 
      ctgcttctg ggaaggctc caaattgct cggacctga 
      ttatcctgt ccatatacc atatggaat aggcaagca 
      tataaaatg gacaaacct caaccccag tcagttagt 
      atccgagtt aaagcaaaa agaatccca gtttgatta 
      tcattcttt agaggatta tttcaagcg tgctggaat 
      aacctctgg atatcagga aaagccgtg tttgaaccc 
      ccgcgaccc ggaatgtcc agtgacggc tccaaatta 
      caattcgag tcaaaattt agatattgg tgctgaact 
      aaccagtgg ttgttacgg tttcgccgc gaaatacat 
      gcactggcc ggaagactt gatcgttgg cggtgccaa 
      taagaataa atctggtca tattaaaca agccaaagc 
      tttacaaac tgttgtaca attaatggg cgaacacga 
      actgttcga attctggtc tggacatta caaagtgca 
      ccacatcgg atggaacca ggagaaggc cacaaccgt 
      actgaacgc ctggcagaa gaagttcgc acaggaggt 
      ggatcgtct gacgaagag caagaagtc gtcgaatta 
      catcttcgt gacgttctc caccgccaa tttgaacaa 
      gatgttgaa ggaggcgtc gaagacgga cgtggtgaa 
      gctgggggt ggtgctggg ggtggcggc ggtgtacgg 
      gtgggtggc ccagtcggg gctggctgc cttgggagt 
      gctggtctt ggcgagctc agccgccgc cgcgctggg 
      cgtttgcag tttgatcgg actgccgat aaacctact 
      gagcacgca tgtgttacc ttttgttac tgttggact 
      cgcaatgag agaaatgtt ccttatttt gtcgacgca 
      tatgaggaa tctgtcgcc accggaagt gctacaaag 
      gacgggtcc tagcattat ctcggcggc gttagtgaa 
      ttccgccgc gttcttagc ggctgctgt actgccagt 
      gccggcgtt acgtgtttt ctgtttaca atgtgcggt 
      acttgttat attccacgg tgctgcatt gcttattgt 
      gttcccttc gctcttggc cctcgagca gcgctgtcg 
      aaagactga cgtgccgtg cttccggaa cgagaccaa 
      gtccaagcc tcccacaaa caacaacaa tgacccgga 
      acatggtga aaacttgtt gactgcgga ggtgatctg 
      tcagcaaaa gaaaagtct tttaacgtg gttagtgaa 
      tcgttactt cacatcagt gcttgttaa accatttgt 
      gaaggtggt tttgtgttt gacgtacgc tgtgttgat 
      aatattttg cgtttttaa cggattgaa gttggagtt 
      tgacgtgag attatcgac atttttacc aaagaatac 
      gcaggagta caaatattt agaagctca aaataaatt 
      ctttggttt ttataattt ttatttagt gacgaccga 
      gttggagta ccctttaag ccaaccgct tctgtatga 
      ataccactc gtctttaac aaatgtacc tcatgtttt 
      gaaagactc gaatggggg cttgaatat gaacgattt 
      ttggttagc caatttccg ggatttttt actagattt 
      acagcaaga gtttgactt gaatcggaa aaacaattt 
      ccttaatag cgagaagtg gtttccgaa tgcgaccga 
      aaaggcagt attagcgtt caaattgtt agcgcagac 
      tggcattgt ggagtttcc agtcgataa aagtcaaat 
      ttttaagaa acaacttgt cgataaagg cattataga 
      tcccaaagc gttttataa ctatttatc agtttggaa 
      tagtaacga tcaaatgtc atattcaag ctcccaggc 
      gaatctaac accgaagcc ttgtcaaca ctatagttc 
      taaggccga gttcgattt aaaaattcc aaagtcacg 
      acctcttgt ttatactca aatgccatt ctacctcaa 
      gaatttgaa aagtactgg aaaaatcat agaaactct 
      caaacagat taaacaaat ttccgaaga gtacaatca 
      aaggggatt aaagaatta tcccattgg acttatatt 
      cgcttattt taatcagtt cctactact agaccgctt 
      gttggctgc ccagctggt actcacgtt actcgcggc 
      cgttctagt agcgtgtgt tcttgtaca gtggacaaa 
      actttggtg ttccgtatt cagcattat aatctgtat 
      gttgttgat gtgtttggt gaacgtaaa tgctttaag 
      tggaacaat aggtgcatt gcattttat aatagtgag 
      tcgcaatat cttccttat cctaacagg tttcttaag 
      cgctttagg aaattgtga acggagact caaaatgtc 
      gttagaatt atacgccga acctatact caaaggcga 
      ttttggact atttgtatg tgtctcagt attagtaac 
      ttcccagtt cgagttcat ccaatgcca ttttttcac 
      cgcattgct ggtatccct gtgcagttc aactatcaa 
      ttctctggt tttcttccc cattttgct actcctcgc 
      cggtcctcg agccgaact ctaccaact agaacacac 
      cgaccggat tgccacacc aacaccacc tccacctcc 
      tcctccccc tatccgaac cccaaccta ctcaaagcc 
      ctcccgccg aaacacttc cgtcaaagc cactcgaga 
      gcccagcct caccactat tacagagga gagttgcaa 
      tcagagtat tatagtcga gccacaagt cactgtaga 
      atacagtag tccagaatc cagttctag tggccctta 
      cacaaccaa agtaaccgc cactgctaa tattaaggt 
      agagttagt tgcccctgt atatagacc taaatgtag 
      taagtgtca cagatcgaa gtgtaagag gcgacagca 
      gtgtcgctg ttgcaagga ctcagacag tagcgatag 
      tagtgccga ggcagaacc gtgccccag tagctagca 
      ttttgttac catttaagg aaattatgt aaaatatgt 
      ctatattgc atatatgta tacttacca tattcactt 
      atttattta aaaagatga tgtaagatg acttctaac 
      tttaaatta tactcaagg tattattta tattacttt 
      taagtaaag aatattttg acaataagc gcgagtttc 
      tttaccccc ctccctaac taaaaacac aaccgacaa