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Gene information
General information
TC004745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6313356..6323959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004745
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03831

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature specific subset lateral not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature ventral mostlynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    leg musculature not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva bristle irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva dorsalcuticularisation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus sometimes duplicated - Penetrance (percentage of animals/eggs on slide): more than 80%
    mesothoracic tracheal opening not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment anteroposterior-axis decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen tracheal opening not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004745 RA
      MASTAASES PGRHESDLY TRPGWVDAA VALDQLDQG 
KASGDRGIL WVRGRIQRQ LRSLGRILD THAGKFLFV 
SVLAIATFS VGLKSMAFH SDIELLWAE PSTGAEATP 
QEMLSTHQM IVQTAVEPD IDLLHPHGL LEHLALLQK 
ATQVTITMF DITWRLKDF CQAPSIPNF DAHFIEQIF 
ENMMPCSII TALDCFWEG SKLLGPDYP VHIPYGIGK 
HIKWTNLNP SQLVSELKQ KESQFDYHS LEDYFKRAG 
ITSGYQEKP CLNPRDPEC PVTAPNYNS SQNLDIGAE 
LTSGCYGFA AKYMHWPED LIVGGANKN KSGHIKQAK 
ALQTVVQLM GEHELFEFW SGHYKVHHI GWNQEKATT 
VLNAWQKKF AQEVDRLTK SKKSSNYIF VTFSTANLN 
KMLKEASKT DVVKLGVVL GVAAVYGWV AQSGLAALG 
VLVLASSAA AALGVCSLI GLPINLLST HVLPFVTVG 
LAMREMFLI LSTHMRNLS PPEVLQRTG PSIISAALV 
NSAAFLAAA VLPVPALRV FCLQCAVLV IFHGAALLI 
VFPSLLALE QRCRKTDVP CFRNETKSK PPTNNNNDP 
EHGENLLTA EVICQQKKS LLTWLVNRY FTSVLVKPF 
VKVVLCLTY AVLIIFCVF NGLKLEFDV RLSTFLPKN 
TQEYKYLEA QNKFFGFYN FYLVTTELE YPLSQPLLY 
EYHSSLTNV PHVLKDSNG GLNMNDFWL ANFRDFLLD 
LQQEFDLNR KNNFLNSEK WFPNATEKA VLAFKLLAQ 
TGIVEFPVD KSQIFKKQL VDKGIIDPK AFYNYLSVW 
NSNDQMSYS SSQANLTPK PCQHYSSKA EFDLKIPKS 
RPLVYTQMP FYLKNLKST GKIIETLKQ IKQISEEYN 
QRGLKNYPI GLIFAYFNQ FLLLDRLLA AQLVLTLLA 
AVLVACVLV QWTKLWCSV FSIIICMLL MCLVNVNAL 
SGTIGALHF IIVSRNIFL ILTGFLSAL GNCERRLKM 
SLELYAEPI LKGDFGLFV CVSVLVTSQ FEFIQCHFF 
TALLVSLCS STINSLVFF PILLLLAGP RAELYQLEH 
TDRIATPTP PPPPPPPIR TPTYSKPSR RNTSVKATR 
EPSLTTITE ESCNQSIIV EPQVTVEYS SPESSSSGP 
YTTKVTATA NIKVELVAP VYRPKCSKC HRSKCKRRQ 
QCRCCKDSD SSDSSAEAE PCPSS
    CDS sequence
    • >TC004745 RA
      atggcctcc acggcggcg tcggagtcg ccggggcgc 
cacgagtcg gacctgtac accaggccg ggatgggtc 
gacgccgcc gtcgcgctg gaccagctc gaccagggc 
aaagcgagc ggagaccgc ggcattctg tgggttcgt 
ggccgaatt cagcgccag ctgcgctcc ctgggccgc 
atcctcgat acgcacgcc ggcaagttc ctcttcgtg 
agcgtgctg gcgatcgcc accttctcc gtgggcctg 
aagagcatg gccttccac tcggacatc gagctgctg 
tgggccgag ccctccacg ggcgccgag gcgacgccg 
caggagatg ctctccacg caccagatg atcgtgcag 
acggccgtc gagcccgac atcgacctc ctgcatccg 
cacggcctc ctggagcac ctcgcgctc ctccagaag 
gccacgcag gtcaccatc acgatgttc gacatcacc 
tggcgcctc aaggacttc tgccaagca cccagcatt 
cccaatttt gatgcgcac tttattgaa cagattttc 
gaaaatatg atgccctgc tccatcatc acggcgctc 
gactgcttc tgggaaggc tccaaattg ctcggacct 
gattatcct gtccatata ccatatgga ataggcaag 
catataaaa tggacaaac ctcaacccc agtcagtta 
gtatccgag ttaaagcaa aaagaatcc cagtttgat 
tatcattct ttagaggat tatttcaag cgtgctgga 
ataacctct ggatatcag gaaaagccg tgtttgaac 
ccccgcgac ccggaatgt ccagtgacg gctccaaat 
tacaattcg agtcaaaat ttagatatt ggtgctgaa 
ctaaccagt ggttgttac ggtttcgcc gcgaaatac 
atgcactgg ccggaagac ttgatcgtt ggcggtgcc 
aataagaat aaatctggt catattaaa caagccaaa 
gctttacaa actgttgta caattaatg ggcgaacac 
gaactgttc gaattctgg tctggacat tacaaagtg 
caccacatc ggatggaac caggagaag gccacaacc 
gtactgaac gcctggcag aagaagttc gcacaggag 
gtggatcgt ctgacgaag agcaagaag tcgtcgaat 
tacatcttc gtgacgttc tccaccgcc aatttgaac 
aagatgttg aaggaggcg tcgaagacg gacgtggtg 
aagctgggg gtggtgctg ggggtggcg gcggtgtac 
gggtgggtg gcccagtcg gggctggct gccttggga 
gtgctggtc ttggcgagc tcagccgcc gccgcgctg 
ggcgtttgc agtttgatc ggactgccg ataaaccta 
ctgagcacg catgtgtta ccttttgtt actgttgga 
ctcgcaatg agagaaatg ttccttatt ttgtcgacg 
catatgagg aatctgtcg ccaccggaa gtgctacaa 
aggacgggt cctagcatt atctcggcg gcgttagtg 
aattccgcc gcgttctta gcggctgct gtactgcca 
gtgccggcg ttacgtgtt ttctgttta caatgtgcg 
gtacttgtt atattccac ggtgctgca ttgcttatt 
gtgttccct tcgctcttg gccctcgag cagcgctgt 
cgaaagact gacgtgccg tgcttccgg aacgagacc 
aagtccaag cctcccaca aacaacaac aatgacccg 
gaacatggt gaaaacttg ttgactgcg gaggtgatc 
tgtcagcaa aagaaaagt cttttaacg tggttagtg 
aatcgttac ttcacatca gtgcttgtt aaaccattt 
gtgaaggtg gttttgtgt ttgacgtac gctgtgttg 
ataatattt tgcgttttt aacggattg aagttggag 
tttgacgtg agattatcg acattttta ccaaagaat 
acgcaggag tacaaatat ttagaagct caaaataaa 
ttctttggt ttttataat ttttattta gtgacgacc 
gagttggag tacccttta agccaaccg cttctgtat 
gaataccac tcgtcttta acaaatgta cctcatgtt 
ttgaaagac tcgaatggg ggcttgaat atgaacgat 
ttttggtta gccaatttc cgggatttt ttactagat 
ttacagcaa gagtttgac ttgaatcgg aaaaacaat 
ttccttaat agcgagaag tggtttccg aatgcgacc 
gaaaaggca gtattagcg ttcaaattg ttagcgcag 
actggcatt gtggagttt ccagtcgat aaaagtcaa 
atttttaag aaacaactt gtcgataaa ggcattata 
gatcccaaa gcgttttat aactattta tcagtttgg 
aatagtaac gatcaaatg tcatattca agctcccag 
gcgaatcta acaccgaag ccttgtcaa cactatagt 
tctaaggcc gagttcgat ttaaaaatt ccaaagtca 
cgacctctt gtttatact caaatgcca ttctacctc 
aagaatttg aaaagtact ggaaaaatc atagaaact 
ctcaaacag attaaacaa atttccgaa gagtacaat 
caaagggga ttaaagaat tatcccatt ggacttata 
ttcgcttat tttaatcag ttcctacta ctagaccgc 
ttgttggct gcccagctg gtactcacg ttactcgcg 
gccgttcta gtagcgtgt gttcttgta cagtggaca 
aaactttgg tgttccgta ttcagcatt ataatctgt 
atgttgttg atgtgtttg gtgaacgta aatgcttta 
agtggaaca ataggtgca ttgcatttt ataatagtg 
agtcgcaat atcttcctt atcctaaca ggtttctta 
agcgcttta ggaaattgt gaacggaga ctcaaaatg 
tcgttagaa ttatacgcc gaacctata ctcaaaggc 
gattttgga ctatttgta tgtgtctca gtattagta 
acttcccag ttcgagttc atccaatgc cattttttc 
accgcattg ctggtatcc ctgtgcagt tcaactatc 
aattctctg gttttcttc cccattttg ctactcctc 
gccggtcct cgagccgaa ctctaccaa ctagaacac 
accgaccgg attgccaca ccaacacca cctccacct 
cctcctccc cctatccga accccaacc tactcaaag 
ccctcccgc cgaaacact tccgtcaaa gccactcga 
gagcccagc ctcaccact attacagag gagagttgc 
aatcagagt attatagtc gagccacaa gtcactgta 
gaatacagt agtccagaa tccagttct agtggccct 
tacacaacc aaagtaacc gccactgct aatattaag 
gtagagtta gttgcccct gtatataga cctaaatgt 
agtaagtgt cacagatcg aagtgtaag aggcgacag 
cagtgtcgc tgttgcaag gactcagac agtagcgat 
agtagtgcc gaggcagaa ccgtgcccc agtagctag
    mRNA sequence
    • >TC004745 RA
      ggtgctatt agccggatt ccactgtac ttacgtttc 
aattgcaca gagaatcgg gttggggct tatcggcgg 
tggtggatg tgtggagtc tattagact gtctgtata 
tcgtatgat ggtgagaaa aaaaacaaa agtaatgta 
atttggaga atttacatt ctaaacata tactgtggg 
tggtcttcg ttcggagaa actcgacgc cggtcgcct 
gtagagagc gacccaagg cattgcgat tacaatgaa 
cgggcaaca gcttagcag tcggctcgt cagtcgaac 
tcgcagttg tgttggttg tggagtcgt gagtcgcac 
tgcgtgtca ctttgttgt tgttcccca cgttaaatg 
tgttttggt cttggccgt ttgtgcgtc cgagtgcga 
gtggcggaa gtgtcgaaa gcagcgtgt ccgaggatg 
cccggtgca cgcgccagg actataggg cccgagccc 
gcgccggag gccagtgcc gtggtctgt ggaatgatg 
cgcggtttg gagcgggcc cgcgcccgc taagaggat 
ggcctccac ggcggcgtc ggagtcgcc ggggcgcca 
cgagtcgga cctgtacac caggccggg atgggtcga 
cgccgccgt cgcgctgga ccagctcga ccagggcaa 
agcgagcgg agaccgcgg cattctgtg ggttcgtgg 
ccgaattca gcgccagct gcgctccct gggccgcat 
cctcgatac gcacgccgg caagttcct cttcgtgag 
cgtgctggc gatcgccac cttctccgt gggcctgaa 
gagcatggc cttccactc ggacatcga gctgctgtg 
ggccgagcc ctccacggg cgccgaggc gacgccgca 
ggagatgct ctccacgca ccagatgat cgtgcagac 
ggccgtcga gcccgacat cgacctcct gcatccgca 
cggcctcct ggagcacct cgcgctcct ccagaaggc 
cacgcaggt caccatcac gatgttcga catcacctg 
gcgcctcaa ggacttctg ccaagcacc cagcattcc 
caattttga tgcgcactt tattgaaca gattttcga 
aaatatgat gccctgctc catcatcac ggcgctcga 
ctgcttctg ggaaggctc caaattgct cggacctga 
ttatcctgt ccatatacc atatggaat aggcaagca 
tataaaatg gacaaacct caaccccag tcagttagt 
atccgagtt aaagcaaaa agaatccca gtttgatta 
tcattcttt agaggatta tttcaagcg tgctggaat 
aacctctgg atatcagga aaagccgtg tttgaaccc 
ccgcgaccc ggaatgtcc agtgacggc tccaaatta 
caattcgag tcaaaattt agatattgg tgctgaact 
aaccagtgg ttgttacgg tttcgccgc gaaatacat 
gcactggcc ggaagactt gatcgttgg cggtgccaa 
taagaataa atctggtca tattaaaca agccaaagc 
tttacaaac tgttgtaca attaatggg cgaacacga 
actgttcga attctggtc tggacatta caaagtgca 
ccacatcgg atggaacca ggagaaggc cacaaccgt 
actgaacgc ctggcagaa gaagttcgc acaggaggt 
ggatcgtct gacgaagag caagaagtc gtcgaatta 
catcttcgt gacgttctc caccgccaa tttgaacaa 
gatgttgaa ggaggcgtc gaagacgga cgtggtgaa 
gctgggggt ggtgctggg ggtggcggc ggtgtacgg 
gtgggtggc ccagtcggg gctggctgc cttgggagt 
gctggtctt ggcgagctc agccgccgc cgcgctggg 
cgtttgcag tttgatcgg actgccgat aaacctact 
gagcacgca tgtgttacc ttttgttac tgttggact 
cgcaatgag agaaatgtt ccttatttt gtcgacgca 
tatgaggaa tctgtcgcc accggaagt gctacaaag 
gacgggtcc tagcattat ctcggcggc gttagtgaa 
ttccgccgc gttcttagc ggctgctgt actgccagt 
gccggcgtt acgtgtttt ctgtttaca atgtgcggt 
acttgttat attccacgg tgctgcatt gcttattgt 
gttcccttc gctcttggc cctcgagca gcgctgtcg 
aaagactga cgtgccgtg cttccggaa cgagaccaa 
gtccaagcc tcccacaaa caacaacaa tgacccgga 
acatggtga aaacttgtt gactgcgga ggtgatctg 
tcagcaaaa gaaaagtct tttaacgtg gttagtgaa 
tcgttactt cacatcagt gcttgttaa accatttgt 
gaaggtggt tttgtgttt gacgtacgc tgtgttgat 
aatattttg cgtttttaa cggattgaa gttggagtt 
tgacgtgag attatcgac atttttacc aaagaatac 
gcaggagta caaatattt agaagctca aaataaatt 
ctttggttt ttataattt ttatttagt gacgaccga 
gttggagta ccctttaag ccaaccgct tctgtatga 
ataccactc gtctttaac aaatgtacc tcatgtttt 
gaaagactc gaatggggg cttgaatat gaacgattt 
ttggttagc caatttccg ggatttttt actagattt 
acagcaaga gtttgactt gaatcggaa aaacaattt 
ccttaatag cgagaagtg gtttccgaa tgcgaccga 
aaaggcagt attagcgtt caaattgtt agcgcagac 
tggcattgt ggagtttcc agtcgataa aagtcaaat 
ttttaagaa acaacttgt cgataaagg cattataga 
tcccaaagc gttttataa ctatttatc agtttggaa 
tagtaacga tcaaatgtc atattcaag ctcccaggc 
gaatctaac accgaagcc ttgtcaaca ctatagttc 
taaggccga gttcgattt aaaaattcc aaagtcacg 
acctcttgt ttatactca aatgccatt ctacctcaa 
gaatttgaa aagtactgg aaaaatcat agaaactct 
caaacagat taaacaaat ttccgaaga gtacaatca 
aaggggatt aaagaatta tcccattgg acttatatt 
cgcttattt taatcagtt cctactact agaccgctt 
gttggctgc ccagctggt actcacgtt actcgcggc 
cgttctagt agcgtgtgt tcttgtaca gtggacaaa 
actttggtg ttccgtatt cagcattat aatctgtat 
gttgttgat gtgtttggt gaacgtaaa tgctttaag 
tggaacaat aggtgcatt gcattttat aatagtgag 
tcgcaatat cttccttat cctaacagg tttcttaag 
cgctttagg aaattgtga acggagact caaaatgtc 
gttagaatt atacgccga acctatact caaaggcga 
ttttggact atttgtatg tgtctcagt attagtaac 
ttcccagtt cgagttcat ccaatgcca ttttttcac 
cgcattgct ggtatccct gtgcagttc aactatcaa 
ttctctggt tttcttccc cattttgct actcctcgc 
cggtcctcg agccgaact ctaccaact agaacacac 
cgaccggat tgccacacc aacaccacc tccacctcc 
tcctccccc tatccgaac cccaaccta ctcaaagcc 
ctcccgccg aaacacttc cgtcaaagc cactcgaga 
gcccagcct caccactat tacagagga gagttgcaa 
tcagagtat tatagtcga gccacaagt cactgtaga 
atacagtag tccagaatc cagttctag tggccctta 
cacaaccaa agtaaccgc cactgctaa tattaaggt 
agagttagt tgcccctgt atatagacc taaatgtag 
taagtgtca cagatcgaa gtgtaagag gcgacagca 
gtgtcgctg ttgcaagga ctcagacag tagcgatag 
tagtgccga ggcagaacc gtgccccag tagctagca 
ttttgttac catttaagg aaattatgt aaaatatgt 
ctatattgc atatatgta tacttacca tattcactt 
atttattta aaaagatga tgtaagatg acttctaac 
tttaaatta tactcaagg tattattta tattacttt 
taagtaaag aatattttg acaataagc gcgagtttc 
tttaccccc ctccctaac taaaaacac aaccgacaa
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