Status
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Gene information
General information
TC030889
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004741,TC004740 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6284540..6295459
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030889
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02952

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs broader - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Specifically the femur, tibiotarsus and pretarsus are affected.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03829

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Tibiotarsus & Femur potentially fused.
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030889 RA
      MQDENENKS LLFLNNSNK YGAMHSDSS GTPKKVIFC 
VHGKPSAGC CKVLQSEVI DIKEGDSVD TPTKENQDA 
TEMINKHCH RGKSPEIDR KARRKLIVA SVLCVLFMI 
GEVIGGYLS NSLAIASDA AHLLTDFAS FMISLFALW 
MATRPSTKT MHFGWYRAE VIGALTSVL LIWVVTGIL 
VYIAVQRVI HKSFDIDSK VMLITSGIG VIVNLIMGL 
TLHQHGHSH GGGSTHSHD NINVRAAFI HVIGDFLQS 
FGVFVAAAV IYFNEQWVI IDPICTFIF SVLVMFTTF 
NIIKDTLTV LMEGAPKGI DFNQVMKTL LNIEGVKRV 
HNLRIWGLS LDKIAMSAH VAISPDVNP QNVLMVATK 
NIHDKYNFF EMTLQIEEF QEAMEDFKA RLRWWIPKL 
LFDSRDNQV GYVTSGGSI TRRSLIGWR DTPPAPKRL 
KFRGSFFDF FTENKVKMP KDIVSCRSP YSRDLTLPD 
LDPPPHFWT RMKQRWNGG HQLLPTTDS PTISACTTS 
TNLSNLEFQ SSSTLNQQI YYGNNLRRA VPEIEPLQP 
HKNNLDMTI NATQQDFEY SNESLSRSK NDKSSITTR 
ALFRQVVAA VAVSWVSMV IGYVSGYTS PAGISLKAD 
LQITDLQFS WISGLMPLA ALLGGLLGG PLIEGLGRK 
WTLLLTNVL FLVSWIINY FAQEYWYLY ISRSISGCG 
VGIASLTLP VYLGETLQP EVRGTLGLL PTAFGNIGI 
LLCFSMGIV SEWKGIAGI GALLAVPFL FVIWFIPET 
PRWYISKNK TDQSRRALE WLRDKNNQD TLEKEFEEL 
LKSQKIADE KADKLKDLY SRPYVKSLL IVLGLMFFQ 
QFSGINAVI FYTTQIFED TGSDIDSSV QTIIVGAVN 
FASTFIATI LIDRLGRKV LLYISSVAM IITLAALGA 
YFYLMTVPD IDIAPYSWM PLASFVVYV LGFSFGFGP 
IPWLMMGEI LPAKIRGPA ASIATGFNW TCTFVVTTT 
FPIFKDIIG AHGTFWLFC AVCVLGLVF TIFWVPETK 
GQSLEDIER KLAGEKVRR MSSVANMKP LQSTF
    CDS sequence
    • >TC030889 RA
      atgcaggat gaaaacgaa aataagtct cttctattt 
ttaaataat agtaacaaa tacggagca atgcacagt 
gacagtagc ggcactcct aaaaaagtg atattttgt 
gttcatgga aaaccaagt gctggttgc tgcaaagtt 
ttacaaagt gaagtcatc gacatcaaa gagggagat 
tcggttgat acaccgacc aaagaaaat caggatgcc 
accgaaatg attaataaa cattgccat cgagggaaa 
tctccggaa attgatcgc aaagctagg cgaaaatta 
attgtagct agtgtatta tgtgtactt ttcatgatc 
ggagaagtt ataggtggc tacctgtca aatagcttg 
gcaatagcc tctgatgcc gctcacctt cttaccgac 
tttgcgagt tttatgata tctctgttt gccttatgg 
atggcaact aggccctcc accaagaca atgcacttt 
gggtggtac cgcgctgaa gtgataggt gctctgact 
tccgtccta ctaatttgg gtagttact ggaatactg 
gtctacatt gccgtacaa agagtcatc cataagtcc 
tttgatatc gatagtaaa gtaatgcta atcacctcc 
ggtatcggc gttattgtg aacttaata atgggttta 
acactccac caacatggg cattcccat ggaggtggt 
agcacacat tcccacgat aacatcaac gtcagagcg 
gcttttatt cacgttatc ggcgacttt ttgcaaagt 
tttggtgtt ttcgtggcc gctgctgtg atttatttc 
aacgaacaa tgggtcata attgacccc atctgtacc 
ttcattttt tcagtatta gtgatgttt accacgttc 
aatattatc aaagatacg ttaacagtg ctgatggaa 
ggagctccc aaaggaatt gatttcaat caagtcatg 
aaaacgctt ctaaatata gaaggagtt aaaagagtt 
cataacttg agaatatgg ggcctaagt ttggataaa 
attgcaatg tctgctcat gttgctatt agtcctgat 
gtgaatcca caaaatgtt ttaatggtc gctactaaa 
aatattcat gataaatac aacttcttt gagatgaca 
ctgcagatc gaggagttc caagaggcc atggaagac 
tttaaggca aggttaagg tggtggata cccaaattg 
ttatttgac tcacgggac aatcaagtt ggctacgtg 
acgtcggga ggctcgatc accaggcgc tcgctgatt 
ggctggcgg gacacacct ccggctccg aaacgtctg 
aaatttcgc gggtcgttt ttcgatttt tttactgaa 
aataaagtg aaaatgcca aaagacatt gtgtcatgc 
cggagtcct tattccagg gatttaacg ctcccagac 
ctggaccca cccccgcac ttctggacc cgaatgaag 
caaagatgg aacggtggt catcagttg cttccaacg 
acagacagc ccaactatc tctgcctgt acgacttcc 
acaaatttg tcgaatttg gaattccag tccagttcg 
actctcaat caacaaatt tactacggg aacaactta 
agaagagcg gtacctgaa atcgaaccc cttcaacct 
cacaagaat aatctcgac atgacaata aatgcaacg 
caacaagat ttcgaatat tccaatgag tctctctct 
agatcaaaa aatgacaag tcctcgatc accaccagg 
gcgctgttt cgccaagtt gtagcagca gttgccgtc 
tcatgggtg tccatggtg attgggtat gtctctggt 
tatacttca ccagctggc atttcctta aaggcagat 
ctccaaata acagactta cagttttcg tggatctca 
ggcctcatg ccattagcc gcccttctt ggagggttg 
ttaggaggg cccttaatt gagggttta ggaagaaaa 
tggactctt ttgttgacc aacgttctc ttcttagtt 
agttggata ataaattat ttcgctcaa gaatactgg 
tacttgtac atcagtcgc agcatctcc ggctgtggc 
gtagggata gcttctttg acccttccg gtttacttg 
ggagagaca ctgcagcca gaagtacgt ggcacttta 
ggcttgttg cccacagct ttcggaaac attgggatt 
ttgttatgt ttttcaatg ggaattgtc tcggaatgg 
aaaggaatc gcgggaatt ggggccttg ctagctgtg 
cctttcctc ttcgtgata tggtttata ccggaaacg 
cccagatgg tacatttcg aagaacaaa accgaccag 
tcacgccgg gcactggaa tggttgcga gacaagaac 
aaccaagac acccttgag aaggagttc gaagagttg 
ctgaaatct caaaaaatt gccgacgaa aaggccgac 
aagcttaaa gatttgtac agtcggcct tacgtaaaa 
tctctactg attgtgctt ggattgatg tttttccaa 
cagttcagc ggaatcaac gctgttatc ttctacacg 
actcaaatt tttgaagat accggttcg gacattgac 
tccagcgtt cagaccata attgttggc gctgttaat 
ttcgcctct acgttcatt gcaactatt ctgatcgac 
agactgggg cgaaaagtt ctcctgtac atttcaagt 
gttgccatg atcataact ttagcagca ctgggggcc 
tacttctat ttaatgacc gtgccagat attgacatt 
gcgccgtac agctggatg ccacttgcc agttttgtc 
gtttatgtg ttgggattt tctttcggc tttgggcca 
attccctgg ctgatgatg ggcgaaatt ttgccggcg 
aaaattcga ggacctgca gcctccata gccacaggg 
tttaactgg acttgcact ttcgtcgta acaacgaca 
tttccgata ttcaaagac attattggg gcgcacggc 
actttctgg ctgttttgc gcagtgtgt gtattagga 
cttgttttt acaatcttc tgggtgcca gaaacgaag 
ggacagagt ttagaggac attgaaaga aaattagcg 
ggtgaaaaa gtgcgaaga atgagttcc gtagcgaac 
atgaagcca ttgcaatct acattttag
    mRNA sequence
    • >TC030889 RA
      tcttgtttg tatttattc aactaggta cctacttga 
aatagataa gaaactgtc gggccgggt gcacaaaca 
ctccttagt tcacaaaga taacaataa cttattgcc 
cagaaactg aacatgaac atattctga cattctgag 
attaatccg tgcccacgt aaatgaaaa acaaactgg 
caagtggtt gaatagcca taaaaatgc aggatgaaa 
acgaaaata agtctcttc tatttttaa ataatagta 
acaaatacg gagcaatgc acagtgaca gtagcggca 
ctcctaaaa aagtgatat tttgtgttc atggaaaac 
caagtgctg gttgctgca aagttttac aaagtgaag 
tcatcgaca tcaaagagg gagattcgg ttgatacac 
cgaccaaag aaaatcagg atgccaccg aaatgatta 
ataaacatt gccatcgag ggaaatctc cggaaattg 
atcgcaaag ctaggcgaa aattaattg tagctagtg 
tattatgtg tacttttca tgatcggag aagttatag 
gtggctacc tgtcaaata gcttggcaa tagcctctg 
atgccgctc accttctta ccgactttg cgagtttta 
tgatatctc tgtttgcct tatggatgg caactaggc 
cctccacca agacaatgc actttgggt ggtaccgcg 
ctgaagtga taggtgctc tgacttccg tcctactaa 
tttgggtag ttactggaa tactggtct acattgccg 
tacaaagag tcatccata agtcctttg atatcgata 
gtaaagtaa tgctaatca cctccggta tcggcgtta 
ttgtgaact taataatgg gtttaacac tccaccaac 
atgggcatt cccatggag gtggtagca cacattccc 
acgataaca tcaacgtca gagcggctt ttattcacg 
ttatcggcg actttttgc aaagttttg gtgttttcg 
tggccgctg ctgtgattt atttcaacg aacaatggg 
tcataattg accccatct gtaccttca ttttttcag 
tattagtga tgtttacca cgttcaata ttatcaaag 
atacgttaa cagtgctga tggaaggag ctcccaaag 
gaattgatt tcaatcaag tcatgaaaa cgcttctaa 
atatagaag gagttaaaa gagttcata acttgagaa 
tatggggcc taagtttgg ataaaattg caatgtctg 
ctcatgttg ctattagtc ctgatgtga atccacaaa 
atgttttaa tggtcgcta ctaaaaata ttcatgata 
aatacaact tctttgaga tgacactgc agatcgagg 
agttccaag aggccatgg aagacttta aggcaaggt 
taaggtggt ggataccca aattgttat ttgactcac 
gggacaatc aagttggct acgtgacgt cgggaggct 
cgatcacca ggcgctcgc tgattggct ggcgggaca 
cacctccgg ctccgaaac gtctgaaat ttcgcgggt 
cgtttttcg attttttta ctgaaaata aagtgaaaa 
tgccaaaag acattgtgt catgccgga gtccttatt 
ccagggatt taacgctcc cagacctgg acccacccc 
cgcacttct ggacccgaa tgaagcaaa gatggaacg 
gtggtcatc agttgcttc caacgacag acagcccaa 
ctatctctg cctgtacga cttccacaa atttgtcga 
atttggaat tccagtcca gttcgactc tcaatcaac 
aaatttact acgggaaca acttaagaa gagcggtac 
ctgaaatcg aaccccttc aacctcaca agaataatc 
tcgacatga caataaatg caacgcaac aagatttcg 
aatattcca atgagtctc tctctagat caaaaaatg 
acaagtcct cgatcacca ccagggcgc tgtttcgcc 
aagttgtag cagcagttg ccgtctcat gggtgtcca 
tggtgattg ggtatgtct ctggttata cttcaccag 
ctggcattt ccttaaagg cagatctcc aaataacag 
acttacagt tttcgtgga tctcaggcc tcatgccat 
tagccgccc ttcttggag ggttgttag gagggccct 
taattgagg gtttaggaa gaaaatgga ctcttttgt 
tgaccaacg ttctcttct tagttagtt ggataataa 
attatttcg ctcaagaat actggtact tgtacatca 
gtcgcagca tctccggct gtggcgtag ggatagctt 
ctttgaccc ttccggttt acttgggag agacactgc 
agccagaag tacgtggca ctttaggct tgttgccca 
cagctttcg gaaacattg ggattttgt tatgttttt 
caatgggaa ttgtctcgg aatggaaag gaatcgcgg 
gaattgggg ccttgctag ctgtgcctt tcctcttcg 
tgatatggt ttataccgg aaacgccca gatggtaca 
tttcgaaga acaaaaccg accagtcac gccgggcac 
tggaatggt tgcgagaca agaacaacc aagacaccc 
ttgagaagg agttcgaag agttgctga aatctcaaa 
aaattgccg acgaaaagg ccgacaagc ttaaagatt 
tgtacagtc ggccttacg taaaatctc tactgattg 
tgcttggat tgatgtttt tccaacagt tcagcggaa 
tcaacgctg ttatcttct acacgactc aaatttttg 
aagataccg gttcggaca ttgactcca gcgttcaga 
ccataattg ttggcgctg ttaatttcg cctctacgt 
tcattgcaa ctattctga tcgacagac tggggcgaa 
aagttctcc tgtacattt caagtgttg ccatgatca 
taactttag cagcactgg gggcctact tctatttaa 
tgaccgtgc cagatattg acattgcgc cgtacagct 
ggatgccac ttgccagtt ttgtcgttt atgtgttgg 
gattttctt tcggctttg ggccaattc cctggctga 
tgatgggcg aaattttgc cggcgaaaa ttcgaggac 
ctgcagcct ccatagcca cagggttta actggactt 
gcactttcg tcgtaacaa cgacatttc cgatattca 
aagacatta ttggggcgc acggcactt tctggctgt 
tttgcgcag tgtgtgtat taggacttg tttttacaa 
tcttctggg tgccagaaa cgaagggac agagtttag 
aggacattg aaagaaaat tagcgggtg aaaaagtgc 
gaagaatga gttccgtag cgaacatga agccattgc 
aatctacat tttagatta aatgactga tttttttat 
ttagacgta agatttgta gatctgtat atacattaa 
attattttt atatgtaaa taaattatt atgttttat
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