Status
Please wait ...
Gene information
General information
TC004726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 3981278..3985684
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004726
    iBeetle screen iB_03827
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004726 RA
      MSYNNSTPH SEIFHYGYS TQSSTYHYI 
      QDGGSLNGD DSNFWSSSP RSDSPSPTS 
      NVQNPLVGS YSYFAGTSN KVSSQQRLS 
      HYRNTDEPL QHVQVPFVR VVKRRTTAN 
      KKERRRTQS INNAYADLR DCIPNVPPD 
      TKLSKIKTL RLATSYINY LVKALESDD 
      PAPGSFTAE LGSMGKKST NNTVTECTN 
      EQVSSKGDI GEDMIKTRK AKGRTGWPQ 
      HVWALELEQ EQAL
    • >TC004726 RB
      MSYNNSTPH SEIFHYGYS TQSSTYHYI 
      QDGGSLNGD DSNFWSSSP RSDSPSPTS 
      NVQNPLVGS YSYFAGTSN KVSSQQRLS 
      HYRNTYTAN IPVADEPLQ HVQVPFVRV 
      VKRRTTANK KERRRTQSI NNAYADLRD 
      CIPNVPPDT KLSKIKTLR LATSYINYL 
      VKALESDDP APGSFTAEL GSMGKKSTN 
      NTVTECTNE QVSSKGDIG EDMIKTRKA 
      KGRTGWPQH VWALELEQE QAL
    CDS sequence
    • >TC004726 RA
      atgtcatac aacaattcg acaccacat tcggaaatt 
      tttcattat ggatattcg acacaatcc tccacttac 
      cactatata caagatggt ggttcgctg aacggtgat 
      gactcgaac ttttggtca agcagccct cggtcagac 
      tcaccgtcc ccaacaagt aatgttcaa aaccccttg 
      gttggttca tattcttat tttgctgga acgtctaac 
      aaagtatct tcccagcaa cgactatct cactataga 
      aataccgat gagcccttg caacacgtc caagttcct 
      ttcgtaagg gtggtaaag cgaagaaca actgcaaat 
      aagaaagaa cgtcgccgt acgcaatcc atcaataac 
      gcttacgcc gatttacgt gactgcatt ccaaatgtt 
      ccaccagac acaaaatta tctaaaatt aaaacatta 
      agattagcc acttcctat attaattat ctcgtaaaa 
      gctttggaa tcggacgat ccagcgccc ggaagtttc 
      acagcagag ctgggttcg atgggcaaa aaatcgaca 
      aacaacacc gtcaccgaa tgcacaaat gaacaagtc 
      tcatcaaag ggcgatatt ggggaagat atgatcaag 
      acgagaaag gcaaaaggg cgaacaggt tggccccag 
      cacgtgtgg gccctggag ctggagcaa gaacaagcg 
      ctgtaa
    • >TC004726 RB
      atgtcatac aacaattcg acaccacat tcggaaatt 
      tttcattat ggatattcg acacaatcc tccacttac 
      cactatata caagatggt ggttcgctg aacggtgat 
      gactcgaac ttttggtca agcagccct cggtcagac 
      tcaccgtcc ccaacaagt aatgttcaa aaccccttg 
      gttggttca tattcttat tttgctgga acgtctaac 
      aaagtatct tcccagcaa cgactatct cactataga 
      aatacctat actgctaat atccctgta gccgatgag 
      cccttgcaa cacgtccaa gttcctttc gtaagggtg 
      gtaaagcga agaacaact gcaaataag aaagaacgt 
      cgccgtacg caatccatc aataacgct tacgccgat 
      ttacgtgac tgcattcca aatgttcca ccagacaca 
      aaattatct aaaattaaa acattaaga ttagccact 
      tcctatatt aattatctc gtaaaagct ttggaatcg 
      gacgatcca gcgcccgga agtttcaca gcagagctg 
      ggttcgatg ggcaaaaaa tcgacaaac aacaccgtc 
      accgaatgc acaaatgaa caagtctca tcaaagggc 
      gatattggg gaagatatg atcaagacg agaaaggca 
      aaagggcga acaggttgg ccccagcac gtgtgggcc 
      ctggagctg gagcaagaa caagcgctg taa
    mRNA sequence
    • >TC004726 RA
      tggtttaca agacaattt taaacacta taattatca 
      agttgaaaa atgtcatac aacaattcg acaccacat 
      tcggaaatt tttcattat ggatattcg acacaatcc 
      tccacttac cactatata caagatggt ggttcgctg 
      aacggtgat gactcgaac ttttggtca agcagccct 
      cggtcagac tcaccgtcc ccaacaagt aatgttcaa 
      aaccccttg gttggttca tattcttat tttgctgga 
      acgtctaac aaagtatct tcccagcaa cgactatct 
      cactataga aataccgat gagcccttg caacacgtc 
      caagttcct ttcgtaagg gtggtaaag cgaagaaca 
      actgcaaat aagaaagaa cgtcgccgt acgcaatcc 
      atcaataac gcttacgcc gatttacgt gactgcatt 
      ccaaatgtt ccaccagac acaaaatta tctaaaatt 
      aaaacatta agattagcc acttcctat attaattat 
      ctcgtaaaa gctttggaa tcggacgat ccagcgccc 
      ggaagtttc acagcagag ctgggttcg atgggcaaa 
      aaatcgaca aacaacacc gtcaccgaa tgcacaaat 
      gaacaagtc tcatcaaag ggcgatatt ggggaagat 
      atgatcaag acgagaaag gcaaaaggg cgaacaggt 
      tggccccag cacgtgtgg gccctggag ctggagcaa 
      gaacaagcg ctgtaacgg ctacgggat gtatgttca 
      tgtgcttta taacctgga agtgtatct taaaagtca 
      tttcatttg tagaaattt cctgaaatt gtatataat 
      atatctatt ttagtcta
    • >TC004726 RB
      tggtttaca agacaattt taaacacta taattatca 
      agttgaaaa atgtcatac aacaattcg acaccacat 
      tcggaaatt tttcattat ggatattcg acacaatcc 
      tccacttac cactatata caagatggt ggttcgctg 
      aacggtgat gactcgaac ttttggtca agcagccct 
      cggtcagac tcaccgtcc ccaacaagt aatgttcaa 
      aaccccttg gttggttca tattcttat tttgctgga 
      acgtctaac aaagtatct tcccagcaa cgactatct 
      cactataga aatacctat actgctaat atccctgta 
      gccgatgag cccttgcaa cacgtccaa gttcctttc 
      gtaagggtg gtaaagcga agaacaact gcaaataag 
      aaagaacgt cgccgtacg caatccatc aataacgct 
      tacgccgat ttacgtgac tgcattcca aatgttcca 
      ccagacaca aaattatct aaaattaaa acattaaga 
      ttagccact tcctatatt aattatctc gtaaaagct 
      ttggaatcg gacgatcca gcgcccgga agtttcaca 
      gcagagctg ggttcgatg ggcaaaaaa tcgacaaac 
      aacaccgtc accgaatgc acaaatgaa caagtctca 
      tcaaagggc gatattggg gaagatatg atcaagacg 
      agaaaggca aaagggcga acaggttgg ccccagcac 
      gtgtgggcc ctggagctg gagcaagaa caagcgctg 
      taacggcta cgggatgta tgttcatgt gctttataa 
      cctggaagt gtatcttaa aagtcattt catttgtag 
      aaatttcct gaaattgta tataatata tctatttta 
      gtcta