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Gene information
General information
TC004720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4468084..4471663
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004720
    iBeetle screen iB_06591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    iBeetle screen iB_03824

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004720 RA
      MENSNLRKK TLIFNVLDF LSGEVGASD LSEERKESI 
      EVAIQCLET AYEISSDDR QNLKKVDLL AQIRLNDAG 
      VQQEQALEA ERQKNLGNT AMKNGEYEE AVRYYSMAI 
      EANPTNPVY FCNRAAAYS RLENNEEAI KDCKQALVL 
      DPTYGKAYG RLGIAYSNL NQWADAVRA YESSLKYDP 
      HNASYQTNL TLARERLFE SMENAAPPQ HRPLDITQF 
      INNEQFLTM AREMMANPD FQNIMSGIM AMSQSGDTN 
      FEALFQAGQ NLASRMQDQ DPRFVENLR RHFDPNNAP 
      APGPDPPKR NDEGSSRDG Q
    CDS sequence
    • >TC004720 RA
      atggaaaat tcaaatcta cgcaaaaag actctaatc 
      ttcaacgtc ctggacttc ttatcaggt gaggtcggc 
      gcttccgac ctctccgag gaacgaaag gaatcaatt 
      gaggtggca attcagtgt ctagaaacc gcttacgaa 
      atttcaagc gacgatcgc caaaattta aaaaaagtt 
      gatctcctc gctcaaatc cgactgaac gatgcgggg 
      gtccaacaa gagcaggcc ctcgaggcg gagcgccag 
      aaaaatttg ggcaacaca gcgatgaaa aacggcgaa 
      tatgaggag gctgtgcgt tactactca atggctatc 
      gaggcaaac cccaccaac ccggtgtac ttttgcaac 
      agggccgcg gcctacagc cgcttagag aacaatgaa 
      gaggctatc aaagactgc aaacaggcc ctggttctc 
      gacccgact tacggcaaa gcctacggt agactgggc 
      attgcctat tcgaactta aaccaatgg gccgatgct 
      gtgcgtgct tacgagtcc tccctcaaa tatgacccc 
      cacaacgcc agttaccaa accaacctt actctagcc 
      cgcgaacgc ctgttcgag tcgatggaa aatgcagca 
      cctccacaa caccgccca cttgacatc acgcagttc 
      attaacaac gagcaattt ttgaccatg gcgcgggaa 
      atgatggcc aatcccgac ttccaaaat ataatgtct 
      ggcattatg gcaatgagc caatcgggg gataccaac 
      tttgaggct ctttttcaa gcggggcag aacttggcc 
      agcaggatg caagaccag gatccccgg tttgttgaa 
      aatttgcgg aggcacttc gaccctaac aacgccccc 
      gcccctgga ccagacccc ccgaagcgc aacgatgag 
      gggtcaagt agagacggg caatag
    mRNA sequence
    • >TC004720 RA
      gccgtcaat tacaagacg agaagttct taacctcaa 
      caaaatttc taaaaatta attccaaca atttccccc 
      aatttaatg tagaaatcg gcgcctgcc tttgccttg 
      aatttctct ttatttttg cagcgttcc gttgaaaac 
      ctgcttttc gtatttcct tgccttttt gtaagtacc 
      ttccgcata accagaaaa tctcataaa agtggcttc 
      gcagtgctc cctattttg caacacgtc gcctataac 
      cattttcac tccattcct tttccaaaa tggaaaatt 
      caaatctac gcaaaaaga ctctaatct tcaacgtcc 
      tggacttct tatcaggtg aggtcggcg cttccgacc 
      tctccgagg aacgaaagg aatcaattg aggtggcaa 
      ttcagtgtc tagaaaccg cttacgaaa tttcaagcg 
      acgatcgcc aaaatttaa aaaaagttg atctcctcg 
      ctcaaatcc gactgaacg atgcggggg tccaacaag 
      agcaggccc tcgaggcgg agcgccaga aaaatttgg 
      gcaacacag cgatgaaaa acggcgaat atgaggagg 
      ctgtgcgtt actactcaa tggctatcg aggcaaacc 
      ccaccaacc cggtgtact tttgcaaca gggccgcgg 
      cctacagcc gcttagaga acaatgaag aggctatca 
      aagactgca aacaggccc tggttctcg acccgactt 
      acggcaaag cctacggta gactgggca ttgcctatt 
      cgaacttaa accaatggg ccgatgctg tgcgtgctt 
      acgagtcct ccctcaaat atgaccccc acaacgcca 
      gttaccaaa ccaacctta ctctagccc gcgaacgcc 
      tgttcgagt cgatggaaa atgcagcac ctccacaac 
      accgcccac ttgacatca cgcagttca ttaacaacg 
      agcaatttt tgaccatgg cgcgggaaa tgatggcca 
      atcccgact tccaaaata taatgtctg gcattatgg 
      caatgagcc aatcggggg ataccaact ttgaggctc 
      tttttcaag cggggcaga acttggcca gcaggatgc 
      aagaccagg atccccggt ttgttgaaa atttgcgga 
      ggcacttcg accctaaca acgcccccg cccctggac 
      cagaccccc cgaagcgca acgatgagg ggtcaagta 
      gagacgggc aatagtgta tagggcaat taattcaaa 
      ttacttgat ttttccttc cacaatcac aatgtgtct 
      cgaaatcgt gtaattttt atcaatctt tatttgact 
      aggtctgtg gtaagggat aatggacag tcattcagt 
      tgtgtttcg atattgttg tcaatctca taaaaataa 
      taagagtgt aactgagta ggggatatt gttgggtga 
      aacaaagac tgtcacctt tgttttatg gcataattt 
      tttttattt aaattgttg acaatgttt gtgaatttt 
      atttcgctg aaaaaagtt ttttcttct tagtgtgct 
      gatttaaat atttttatt ggttttgag ggtagaaat 
      gttcgtaaa cgtataatt tgttcttat gttgtttat 
      tgtaaagtt cacattaaa ttttttttg ttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY