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Gene information
General information
TC004714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4540121..4542063
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004714
    iBeetle screen iB_03822

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    maxillary palp elongated - Number of animals showing the phenotype: 8
    maxillary palp thinner - Number of animals showing the phenotype: 8

    labial palp elongated - Number of animals showing the phenotype: 8

    wings bent - Number of animals showing the phenotype: 6

    female genital lobes shortened - Number of animals showing the phenotype: 8
    female genital lobes rounded - Number of animals showing the phenotype: 8

    Adult morphology
    club cuticularisation not present - Number of animals showing the phenotype: 9


    maxillary palp elongated - Number of animals showing the phenotype: 8

    labial palp elongated - Number of animals showing the phenotype: 8


    maxillary palp thinner - Number of animals showing the phenotype: 8


    elytra not closed - Number of animals showing the phenotype: 9


    legs sometimes not present - Number of animals showing the phenotype: 4


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3
    mature egg deposition 3 number 2nd lateral oviduct: 2 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004714 RA
      MSIIPEDGD LEEYFREYS DMYYEETAL DIWNNCVVL 
      SILSVVPSV LSLVVPNLV FGCLVSTGF IPLSVLHFS 
      TGCIGIYLV STLESHFGK IVLLFCWII SYVLLYTGF 
      QLKKYSKSS ISLKLTFII LLVLCEYYL IEAEVWLQI 
      RGIVMVLVM KVLSLADDI NKLDAMPGF IQYFGYTLC 
      GANVMFGPW ISFSDYLNF YKKPTKKNL IWICAIVKV 
      LTTSLFFLT LSNCWSGYI ISDDANRWL IAYKEAFAF 
      RTSHYFISY LAEVGMIAA GFTNSSKRD SLNQYRFVI 
      TEPLEIEFP SSLSIVVKK WNQPMHDFL KKYVYYSWL 
      GFGKFYAIL ATFLISSLL HGLELKVSM VLVSIGVFS 
      YLQVVPRDR IAEIFDYCV RVRPCKDCK HKYKRDNIR 
      VRIILLIYS LATVFHLAY LGVLMDPST NEIGIYEKW 
      QNLYFISHI VMFFNVMVI L
    CDS sequence
    • >TC004714 RA
      atgtccata atcccggaa gatggagat ctggaagaa 
      tactttcgt gaatactct gatatgtac tatgaagaa 
      accgcctta gatatttgg aacaattgt gttgtactc 
      tctatttta agtgtagtc ccttccgtc ctctcttta 
      gttgtacca aatttagta tttggatgt ctggtgtcc 
      acgggtttc atccccttg tcagtatta catttcagc 
      accggttgc attggtatt tatttagtg tcaacgctt 
      gaatctcat tttgggaaa attgtttta ttattttgt 
      tggattatt tcgtacgtt ttgctctat acgggcttc 
      caattgaaa aaatactct aaaagctcc atatcccta 
      aaactcacc tttataatc ctcttagtg ctatgcgaa 
      tattacttg attgaggca gaagtgtgg cttcaaatt 
      cgcggaatc gtcatggtt ttggtaatg aaggttttg 
      tcgttagct gacgatata aacaaatta gacgcaatg 
      ccgggcttt attcagtat tttgggtac acgttatgt 
      ggggccaat gtcatgttt gggccctgg atttcattt 
      agcgattac ttgaatttt tacaagaag cctacgaaa 
      aagaatctc atttggatt tgtgcaatt gttaaagtc 
      ctcaccacg tcgttgttt tttctaact ttgtcaaac 
      tgttggagc ggttacatc atatcagat gacgcgaat 
      cgatggctg atagcatac aaagaagca tttgcgttc 
      aggacaagt cattacttt attagttat ttggcagaa 
      gttggaatg atcgccgct ggtttcacg aactcctca 
      aagcgcgat tctttgaat cagtacaga ttcgtcata 
      accgagcct ctggagatc gaattcccg tcatctctg 
      tcaatagtc gtcaagaaa tggaaccaa ccgatgcac 
      gattttctc aaaaaatat gtttattat tcgtggttg 
      ggtttcggg aagttttac gcaattctc gcaaccttc 
      ctaatttct tcgttactc cacggcctt gaacttaaa 
      gtctccatg gttttagtc tcgattggg gtattctcg 
      tatttacaa gtggtgcca agggaccga attgccgaa 
      atatttgat tattgcgtg agagtccgt ccttgtaaa 
      gactgtaaa cataaatat aaacgtgat aatattaga 
      gttaggatt attttactc atttattcg ctcgcgaca 
      gtattccat ttggcttat ttaggggtc ctcatggac 
      ccctcaacc aacgaaatc ggaatctac gagaaatgg 
      cagaatctc tactttatt agtcacatc gtaatgttc 
      ttcaatgta atggtcatt ttgtga
    mRNA sequence
    • >TC004714 RA
      accttaaac attagacaa aacgaattt ttccttaaa 
      ttactgaaa taatgtgat aataatgtc cataatccc 
      ggaagatgg agatctgga agaatactt tcgtgaata 
      ctctgatat gtactatga agaaaccgc cttagatat 
      ttggaacaa ttgtgttgt actctctat tttaagtgt 
      agtcccttc cgtcctctc tttagttgt accaaattt 
      agtatttgg atgtctggt gtccacggg tttcatccc 
      cttgtcagt attacattt cagcaccgg ttgcattgg 
      tatttattt agtgtcaac gcttgaatc tcattttgg 
      gaaaattgt tttattatt ttgttggat tatttcgta 
      cgttttgct ctatacggg cttccaatt gaaaaaata 
      ctctaaaag ctccatatc cctaaaact cacctttat 
      aatcctctt agtgctatg cgaatatta cttgattga 
      ggcagaagt gtggcttca aattcgcgg aatcgtcat 
      ggttttggt aatgaaggt tttgtcgtt agctgacga 
      tataaacaa attagacgc aatgccggg ctttattca 
      gtattttgg gtacacgtt atgtggggc caatgtcat 
      gtttgggcc ctggatttc atttagcga ttacttgaa 
      tttttacaa gaagcctac gaaaaagaa tctcatttg 
      gatttgtgc aattgttaa agtcctcac cacgtcgtt 
      gttttttct aactttgtc aaactgttg gagcggtta 
      catcatatc agatgacgc gaatcgatg gctgatagc 
      atacaaaga agcatttgc gttcaggac aagtcatta 
      ctttattag ttatttggc agaagttgg aatgatcgc 
      cgctggttt cacgaactc ctcaaagcg cgattcttt 
      gaatcagta cagattcgt cataaccga gcctctgga 
      gatcgaatt cccgtcatc tctgtcaat agtcgtcaa 
      gaaatggaa ccaaccgat gcacgattt tctcaaaaa 
      atatgttta ttattcgtg gttgggttt cgggaagtt 
      ttacgcaat tctcgcaac cttcctaat ttcttcgtt 
      actccacgg ccttgaact taaagtctc catggtttt 
      agtctcgat tggggtatt ctcgtattt acaagtggt 
      gccaaggga ccgaattgc cgaaatatt tgattattg 
      cgtgagagt ccgtccttg taaagactg taaacataa 
      atataaacg tgataatat tagagttag gattatttt 
      actcattta ttcgctcgc gacagtatt ccatttggc 
      ttatttagg ggtcctcat ggacccctc aaccaacga 
      aatcggaat ctacgagaa atggcagaa tctctactt 
      tattagtca catcgtaat gttcttcaa tgtaatggt 
      cattttgtg aataaaaat agctaaaac cacactttc 
      ttccttcat tatttaacc catgaaact aatttcccc 
      aagttttct aatcaaaaa acttttata tggaaaagt 
      tcccaaacc gctacgcgt ttcgacagt taagtggca 
      tcatcaggt tataattta attgaatta gtttcgtta 
      aaaacaaaa caataagta tatttaaaa tttaccgag 
      tacgtaata ctttcttct ttaaaataa attattaaa 
      gctgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY