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Gene information
General information
TC004713 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 847728..856099
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004713
    iBeetle screen iB_03821

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostlynot present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: It seems that only one specific muscle in the head is still present.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004713 RA
      MILNIWYLF AFATCTKAL ISNPHTSSN QTEDNAPYF 
      TKRNLPVLV SGRQGQSSI LKCGAKGTP KPQISWYKG 
      TNLITQSNK KYHLIHQNL KILNLQSED NGLYKCLVC 
      NEVSCIHHN YNLVVEPFY AKSFANNDL EEKTTSDSF 
      RTQKVKLHF RRPNKMPKT LSVRINRRV ILNCRTFGT 
      PSPTISWYK NGEALAKSR RKIVQSRWS MLVKNFNRG 
      DSGNYTCVA CNSMDCINH TFTLSTKEL CGSNAIYSK 
      KSAVVSGSN VTLACRAGA AWVRTYPNG TNQVIKNNT 
      RCLNIDNIS QEDEGWYRC QRVSWYLTV IDSEEEENT 
      VPASSLRKK RETEKKAPY WVNPKKMSK MQVKPAGNM 
      VNLKCKAGG NPTPNITWY KDDKPPNRQ LGDIRYQHW 
      SLTLEDLVT GDTGDYTCV VCNELGCIN FTYRVDVIE 
      RYHNKPVLT HPLENTTAV VGTSINLTC TFLADLNPF 
      IQWTREYPN KSAVVVLQK SGDTDSPEV YEIKNVTYQ 
      DEGWYVCIA ANSLGQTAS KGYLKVVDS LEVEPKNNY 
      IVYTALIIF FTIIAFGTL VLLFFRKLK IEKQKKMID 
      IETVRAAVV TQWTKKVII EKIQNTNED VSEPLLMPV 
      VKIEKQKSQ NNNSGDGMI SEYELPMDS DWEIPRSML 
      CLGKSLGEG AFGKVVKAE AVGLLKPGA SSIVAVKML 
      KEGHTDNEM MDLVSEMEM MKMIGKHNN IINLLGCCT 
      QGGPLYVVV EYAPYGNLR DFLRQHRPS SGYEPAIGL 
      EEKEKKTLT QKDLVSFAY QVARGMEYL ASRRCIHRD 
      LAARNVLVS DDYFLKIAD FGLARDIHC NDYYRKTTD 
      GRLPVKWMA PEALFHRVY TTQSDVWSY GILLWEIMT 
      LGGTPYPSV PSVEKLFQL LRNGHRMEK PPCCSLEIY 
      MLMRECWSY KPNERPTFS QLVEDLDRI LTITANEEY 
      LDLGLPQLD TPPSSQESS GDEEDFPFD SLTSQCLNN 
      VQLS
    CDS sequence
    • >TC004713 RA
      atgatcctc aatatttgg tacctcttc gcctttgcg 
      acttgcacc aaggcactg atatcaaac ccacacaca 
      tcttcaaat caaaccgaa gacaatgcg ccatatttt 
      acaaaacgc aacttacct gttctagta tcaggaagg 
      caaggccaa tctagtata cttaaatgt ggggctaaa 
      ggtacccca aaaccccag atttcatgg tacaaaggc 
      accaacctc atcacccaa agcaataaa aagtatcac 
      ctcattcac caaaacctt aaaatccta aacctccaa 
      tctgaagac aacggtttg tacaaatgt cttgtttgt 
      aacgaagtc agctgcatc catcacaat tacaacctc 
      gttgttgaa ccattttat gcaaaaagt tttgctaac 
      aatgatctc gaagaaaaa accacttcc gattccttt 
      cggacccaa aaagtgaag ctacacttt cgtagaccg 
      aataaaatg ccgaaaaca ttatcagta cggatcaat 
      agacgagta attttaaat tgtagaacg tttggaact 
      cctagtcct actatatcc tggtacaaa aatggcgaa 
      gcattggcg aaaagtcgg agaaaaatt gttcaaagt 
      cggtggtca atgctcgta aaaaacttc aatcgagga 
      gattcagga aattatact tgtgtggct tgcaattct 
      atggattgt atcaatcac acgtttact ttatcgacg 
      aaggaactt tgtggttcc aatgctatt tattcaaaa 
      aaatccgct gtagtttcg ggttctaat gtaactcta 
      gcctgcaga gcgggggcg gcttgggta aggacgtac 
      ccaaatggc acaaatcag gtcatcaaa aataatacc 
      aggtgcctc aatattgac aatatatct caggaagac 
      gaagggtgg tacaggtgt caaagagtt agctggtat 
      ttaacggtt atagatagt gaagaggag gagaacact 
      gtgcctgca tcctctctt cggaaaaaa cgtgaaacg 
      gagaagaaa gccccctat tgggtgaac ccgaaaaaa 
      atgtccaaa atgcaggtg aaaccagct ggtaacatg 
      gtgaacctc aaatgcaaa gctgggggt aacccaact 
      cccaacatc acttggtac aaagacgac aagcctcca 
      aaccgccag ttgggcgat attcggtac caacactgg 
      agcttgact ttggaggac ctcgtcact ggtgacacc 
      ggtgactac acttgtgta gtgtgcaac gaactgggt 
      tgtattaat tttacgtac agagttgac gttatcgaa 
      cgctatcac aacaaacct gttcttaca catccatta 
      gaaaacacg actgcggtt gtgggcaca agtataaat 
      ctcacatgt accttcctt gctgatcta aaccctttt 
      attcagtgg actagggaa tacccaaat aagagtgcg 
      gttgtggtg ctacagaaa agtggcgac actgacagc 
      cctgaagtg tacgaaata aaaaatgta acttatcaa 
      gacgagggc tggtacgta tgcattgca gctaatagc 
      ttaggccaa acggcctca aaaggatat ctaaaagtt 
      gtagatagc ttagaagtt gaaccaaaa aataattac 
      attgtctac actgctctg atcattttc tttacaata 
      attgccttt ggaaccctg gtcctacta ttctttaga 
      aagttgaag attgaaaag cagaaaaaa atgattgat 
      atcgaaaca gttcgtgca gctgttgta actcaatgg 
      actaagaaa gttattatt gaaaaaata caaaacacc 
      aacgaggat gtctccgaa cctttgtta atgccggtc 
      gttaaaata gaaaaacaa aaaagtcaa aacaacaat 
      tccggagac ggaatgatc tctgagtat gagctacca 
      atggactcc gactgggaa attccaaga tcaatgctt 
      tgtctcggg aaaagttta ggcgaggga gcttttggt 
      aaagtagtc aaagctgaa gctgtaggt ttgctcaaa 
      cctggggca tcaagtata gtcgcggta aaaatgttg 
      aaagagggt cacactgac aacgaaatg atggacttg 
      gtgtcggaa atggaaatg atgaaaatg ataggcaaa 
      cacaacaat attattaat ttgctgggc tgttgtacc 
      cagggtggg cccttatac gttgtggta gaatacgct 
      ccttatggc aacttgagg gattttctt cgccaacac 
      agaccatca tctggttac gaaccagcg atcggactt 
      gaagaaaaa gagaagaaa accttgact cagaaagat 
      ttggtgtct tttgcctat caggtggca cgaggaatg 
      gaatatttg gcatcgcga cgatgtatt catcgagat 
      ttagctgcg cggaacgtt ttagtgagc gatgactat 
      tttttgaaa attgctgac tttggcctg gcccgggac 
      atccactgt aacgattat tacagaaaa acaaccgat 
      ggtcgactt ccggttaaa tggatggcg cccgaggcc 
      cttttccac agggtgtac accacccag tcagacgtt 
      tggtcgtac ggcatcctg ctgtgggag ataatgaca 
      cttggaggt actccatat ccgtctgtc ccgagtgtc 
      gaaaaactc ttccagctg ttacgtaat gggcaccgt 
      atggaaaag ccgccctgt tgttcgttg gaaatttac 
      atgttgatg cgcgaatgt tggagctac aaaccgaac 
      gaaaggccc actttttcg caactggtg gaggacctt 
      gacagaatt ttgacaatc acagcgaat gaagagtac 
      ctagatttg gggctcccc caattggac actcctcca 
      tcgagccag gaatcgagc ggcgacgag gaggatttc 
      cctttcgat agtctaact agtcagtgt ctcaataac 
      gtgcagctt tcgtga
    mRNA sequence
    • >TC004713 RA
      tggtagaaa tagacttgt agttaagaa cagaacaga 
      agtcgcatc tcgcccacg ccagacgtc gtctgcgtc 
      cgtagtact cctcagaga gtgccagag taaagtcag 
      agagtaaat cgcagccta ttgtccgcg cgtgtcaac 
      ctttatttt cattcataa acccgcatg tttcgaaaa 
      tgcggtcct tccgaggga ttaaacccg gccagtttt 
      gagcggagc tagaggagt gcggagtga tcttggacc 
      gaaatagaa gtgattttg tgcactctg gacgatttg 
      cgaccgatt ctgatggag acatcatga tcctcaata 
      tttggtacc tcttcgcct ttgcgactt gcaccaagg 
      cactgatat caaacccac acacatctt caaatcaaa 
      ccgaagaca atgcgccat attttacaa aacgcaact 
      tacctgttc tagtatcag gaaggcaag gccaatcta 
      gtatactta aatgtgggg ctaaaggta ccccaaaac 
      cccagattt catggtaca aaggcacca acctcatca 
      cccaaagca ataaaaagt atcacctca ttcaccaaa 
      accttaaaa tcctaaacc tccaatctg aagacaacg 
      gtttgtaca aatgtcttg tttgtaacg aagtcagct 
      gcatccatc acaattaca acctcgttg ttgaaccat 
      tttatgcaa aaagttttg ctaacaatg atctcgaag 
      aaaaaacca cttccgatt cctttcgga cccaaaaag 
      tgaagctac actttcgta gaccgaata aaatgccga 
      aaacattat cagtacgga tcaatagac gagtaattt 
      taaattgta gaacgtttg gaactccta gtcctacta 
      tatcctggt acaaaaatg gcgaagcat tggcgaaaa 
      gtcggagaa aaattgttc aaagtcggt ggtcaatgc 
      tcgtaaaaa acttcaatc gaggagatt caggaaatt 
      atacttgtg tggcttgca attctatgg attgtatca 
      atcacacgt ttactttat cgacgaagg aactttgtg 
      gttccaatg ctatttatt caaaaaaat ccgctgtag 
      tttcgggtt ctaatgtaa ctctagcct gcagagcgg 
      gggcggctt gggtaagga cgtacccaa atggcacaa 
      atcaggtca tcaaaaata ataccaggt gcctcaata 
      ttgacaata tatctcagg aagacgaag ggtggtaca 
      ggtgtcaaa gagttagct ggtatttaa cggttatag 
      atagtgaag aggaggaga acactgtgc ctgcatcct 
      ctcttcgga aaaaacgtg aaacggaga agaaagccc 
      cctattggg tgaacccga aaaaaatgt ccaaaatgc 
      aggtgaaac cagctggta acatggtga acctcaaat 
      gcaaagctg ggggtaacc caactccca acatcactt 
      ggtacaaag acgacaagc ctccaaacc gccagttgg 
      gcgatattc ggtaccaac actggagct tgactttgg 
      aggacctcg tcactggtg acaccggtg actacactt 
      gtgtagtgt gcaacgaac tgggttgta ttaatttta 
      cgtacagag ttgacgtta tcgaacgct atcacaaca 
      aacctgttc ttacacatc cattagaaa acacgactg 
      cggttgtgg gcacaagta taaatctca catgtacct 
      tccttgctg atctaaacc cttttattc agtggacta 
      gggaatacc caaataaga gtgcggttg tggtgctac 
      agaaaagtg gcgacactg acagccctg aagtgtacg 
      aaataaaaa atgtaactt atcaagacg agggctggt 
      acgtatgca ttgcagcta atagcttag gccaaacgg 
      cctcaaaag gatatctaa aagttgtag atagcttag 
      aagttgaac caaaaaata attacattg tctacactg 
      ctctgatca ttttcttta caataattg cctttggaa 
      ccctggtcc tactattct ttagaaagt tgaagattg 
      aaaagcaga aaaaaatga ttgatatcg aaacagttc 
      gtgcagctg ttgtaactc aatggacta agaaagtta 
      ttattgaaa aaatacaaa acaccaacg aggatgtct 
      ccgaacctt tgttaatgc cggtcgtta aaatagaaa 
      aacaaaaaa gtcaaaaca acaattccg gagacggaa 
      tgatctctg agtatgagc taccaatgg actccgact 
      gggaaattc caagatcaa tgctttgtc tcgggaaaa 
      gtttaggcg agggagctt ttggtaaag tagtcaaag 
      ctgaagctg taggtttgc tcaaacctg gggcatcaa 
      gtatagtcg cggtaaaaa tgttgaaag agggtcaca 
      ctgacaacg aaatgatgg acttggtgt cggaaatgg 
      aaatgatga aaatgatag gcaaacaca acaatatta 
      ttaatttgc tgggctgtt gtacccagg gtgggccct 
      tatacgttg tggtagaat acgctcctt atggcaact 
      tgagggatt ttcttcgcc aacacagac catcatctg 
      gttacgaac cagcgatcg gacttgaag aaaaagaga 
      agaaaacct tgactcaga aagatttgg tgtcttttg 
      cctatcagg tggcacgag gaatggaat atttggcat 
      cgcgacgat gtattcatc gagatttag ctgcgcgga 
      acgttttag tgagcgatg actattttt tgaaaattg 
      ctgactttg gcctggccc gggacatcc actgtaacg 
      attattaca gaaaaacaa ccgatggtc gacttccgg 
      ttaaatgga tggcgcccg aggcccttt tccacaggg 
      tgtacacca cccagtcag acgtttggt cgtacggca 
      tcctgctgt gggagataa tgacacttg gaggtactc 
      catatccgt ctgtcccga gtgtcgaaa aactcttcc 
      agctgttac gtaatgggc accgtatgg aaaagccgc 
      cctgttgtt cgttggaaa tttacatgt tgatgcgcg 
      aatgttgga gctacaaac cgaacgaaa ggcccactt 
      tttcgcaac tggtggagg accttgaca gaattttga 
      caatcacag cgaatgaag agtacctag atttggggc 
      tcccccaat tggacactc ctccatcga gccaggaat 
      cgagcggcg acgaggagg atttccctt tcgatagtc 
      taactagtc agtgtctca ataacgtgc agctttcgt 
      gattgactg attccaaat tcggactcg ttttttact 
      aaatgtgtt actattttg taatctaat cggaggtaa 
      atgttacac atgtgtatt tcatggttt tcagttcac 
      gttttatta ataccacca aagaaaatg caattttag 
      tgttaagtg gttacgtta aggttaaga ctgtaaatt 
      tatgtatta ataaaattt cgtaactca c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY