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Gene information
General information
TC004711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 882042..886569
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03820

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC004711 RA
    CDS sequence
    • >TC004711 RA
      atgaacaac tacttcaag ctatttttt cgccggata 
      tcgaaagat ttccagcag aagcagctc ctgccactc 
      aagctgctg tttttcatg cattcctca accgtgctg 
      gtcttgtac ccatatttg accatccac atgcgcgag 
      ttggggata aacgttgaa gaagcggcg acgatgtcc 
      gctgtggca cctgttata gctgttttg atgccgcct 
      ttggcagga ctgattgcc gacaagatt ggcaacttt 
      agggtgatg ttggccctg ttttcggcc atcggcgga 
      gcctcggct ctcctcttg ttgctagtc ccagtcggt 
      agaatcaca ataacttat cctgaccga gtagtattt 
      ggaatgtct tgcagtaca ggcagccct ctgtctctg 
      tcactctac caacaccat cagtgcaca ccgttagga 
      aaccaaaat cgcacggac cttcagctt gaatcttgc 
      ggttttgct tgtcatgct gttctagca gaaaaccag 
      accgaggcg attcttaaa acaaacacc tacagtctg 
      cagttatac aacctcgag aagaactca acacacacc 
      tacacctac accttaaca gaagacgac atacatatc 
      ccggaacta atctccgaa agcctcaac cacaaaacg 
      ctaaaaaac aacgaccga ttcctaaca tcaatccgg 
      cgactcgcc aaaaactcg tactatttc ccgacggcg 
      agccttttt aatttttcc tgcgacctc tcctcgaat 
      tcgtcgata gactgtgtt tttggcacc cgcagctct 
      attgatctc ttcggcctg aaactgggc cccaagttc 
      ggaggaatc ctgcacccg gttgaaacc tccgaagac 
      gacacactt gaggaacgc caaatctac gatttggcg 
      cttgataag agccgcaac acgtcttgc ttgacaggt 
      tttgcccaa cctggactc cacatcact gttaatatc 
      ccaataaga aaagcaaac tcgagcgag gtttccaaa 
      cacctggag ctggggagc tgctcaacg cggtgtttg 
      gtgacaggg gaccggaaa aaagtctgt tcgaaccaa 
      aacagagtc atcgagctt gacatgcaa ctgacgttt 
      tggtcgtat ctagtaatt cgggtgttt attgggatt 
      gtgagcggc acctccttc gcgatgttt gaaggggcg 
      gtgatcgcg atcctgcgg gagcataag gccgattac 
      gggctgcaa aggatttac gcaacgatt ggtggcatg 
      attagctcg ccgctgtcg gggtttttc atcgacttt 
      gccagtcgc gggaaaggg tatacggac tttacccca 
      attttcttc ttttacgca acacttaaa atcgtgagt 
      ggttttctg atgcttttc attaatttg gagttcaag 
      aagccggcg caaagtgta atttcggac gtgttggcg 
      gtacttaga aaaatggaa ctgattgtg cttttcgtg 
      gcgtgcttg atattaggc tcggcgtgg ggttacatt 
      gagagtttc ttgttttgg ttgttgcaa gatttaggt 
      ggttctaaa tctttgatg ggtctaact ataacagtt 
      ggtggtctt gttgggatt ccactctta gttttgtct 
      gggcctata attaagaag attggacat gccaacgtt 
      atttttata ggtttcgct ttctacgcc gtcagatta 
      attggttac tctcttatt tacaatcct tggttgtgc 
      ctgattttt gaggcaatg gaatcggtt actttcggt 
      ttaagtttc acggctgct gtaacttac gctgcgaag 
      ttatcgact gtgactact gacaccacc atacaagga 
      ttattagga ggcttgtat tttgcagtt ggtaaaggc 
      attggtagt ttgattggt gggcatatg attaaaatg 
      tatggaatt cggtcaacg tttcaaaga tttgcaatg 
      tttaccgcg gctaccggt ttagcctat tttgcattt 
      tatcacttg tatatgaag aagagacca tctgccggt 
      acagatatt acaaaaaaa gatgttgaa aaaccaccg 
      caaggcttc gtcgatgtt gatttaaac gccaaggct 
      cctgcccat catgacgtt cctgctgta tacgaagac 
      gctttggcg aaccctgct ttcgaagac accgaattt 
      acggaagaa acgaatcaa gagaaaaac aaagacgtc 
      aatcccaac acgagctaa
    mRNA sequence
    • >TC004711 RA
      agagtataa ttacagtaa tcgtaatga aatcggcga 
      ctcttagtg agaagcatg atggcaacg cgagttcca 
      aactagtat ggtcgcaac ttgccttct gaaccgtca 
      ctgttcgat tacacactc accaattgc tttcatccg 
      cttcgagtt tcaaattgt atttctagg tgtgctcta 
      aactattgt tatttgcat ctgatttaa tcaaattgt 
      gaagtttaa agtgactcg ttatagaaa tccggcaag 
      tttggtgtt attaagacg caatgaaca actacttca 
      agctatttt ttcgccgga tatcgaaag atttccagc 
      agaagcagc tcctgccac tcaagctgc tgtttttca 
      tgcattcct caaccgtgc tggtcttgt acccatatt 
      tgaccatcc acatgcgcg agttgggga taaacgttg 
      aagaagcgg cgacgatgt ccgctgtgg cacctgtta 
      tagctgttt tgatgccgc ctttggcag gactgattg 
      ccgacaaga ttggcaact ttagggtga tgttggccc 
      tgttttcgg ccatcggcg gagcctcgg ctctcctct 
      tgttgctag tcccagtcg gtagaatca caataactt 
      atcctgacc gagtagtat ttggaatgt cttgcagta 
      caggcagcc ctctgtctc tgtcactct accaacacc 
      atcagtgca caccgttag gaaaccaaa atcgcacgg 
      accttcagc ttgaatctt gcggttttg cttgtcatg 
      ctgttctag cagaaaacc agaccgagg cgattctta 
      aaacaaaca cctacagtc tgcagttat acaacctcg 
      agaagaact caacacaca cctacacct acaccttaa 
      cagaagacg acatacata tcccggaac taatctccg 
      aaagcctca accacaaaa cgctaaaaa acaacgacc 
      gattcctaa catcaatcc ggcgactcg ccaaaaact 
      cgtactatt tcccgacgg cgagccttt ttaattttt 
      cctgcgacc tctcctcga attcgtcga tagactgtg 
      tttttggca cccgcagct ctattgatc tcttcggcc 
      tgaaactgg gccccaagt tcggaggaa tcctgcacc 
      cggttgaaa cctccgaag acgacacac ttgaggaac 
      gccaaatct acgatttgg cgcttgata agagccgca 
      acacgtctt gcttgacag gttttgccc aacctggac 
      tccacatca ctgttaata tcccaataa gaaaagcaa 
      actcgagcg aggtttcca aacacctgg agctgggga 
      gctgctcaa cgcggtgtt tggtgacag gggaccgga 
      aaaaagtct gttcgaacc aaaacagag tcatcgagc 
      ttgacatgc aactgacgt tttggtcgt atctagtaa 
      ttcgggtgt ttattggga ttgtgagcg gcacctcct 
      tcgcgatgt ttgaagggg cggtgatcg cgatcctgc 
      gggagcata aggccgatt acgggctgc aaaggattt 
      acgcaacga ttggtggca tgattagct cgccgctgt 
      cggggtttt tcatcgact ttgccagtc gcgggaaag 
      ggtatacgg actttaccc caattttct tcttttacg 
      caacactta aaatcgtga gtggttttc tgatgcttt 
      tcattaatt tggagttca agaagccgg cgcaaagtg 
      taatttcgg acgtgttgg cggtactta gaaaaatgg 
      aactgattg tgcttttcg tggcgtgct tgatattag 
      gctcggcgt ggggttaca ttgagagtt tcttgtttt 
      ggttgttgc aagatttag gtggttcta aatctttga 
      tgggtctaa ctataacag ttggtggtc ttgttggga 
      ttccactct tagttttgt ctgggccta taattaaga 
      agattggac atgccaacg ttattttta taggtttcg 
      ctttctacg ccgtcagat taattggtt actctctta 
      tttacaatc cttggttgt gcctgattt ttgaggcaa 
      tggaatcgg ttactttcg gtttaagtt tcacggctg 
      ctgtaactt acgctgcga agttatcga ctgtgacta 
      ctgacacca ccatacaag gattattag gaggcttgt 
      attttgcag ttggtaaag gcattggta gtttgattg 
      gtgggcata tgattaaaa tgtatggaa ttcggtcaa 
      cgtttcaaa gatttgcaa tgtttaccg cggctaccg 
      gtttagcct attttgcat tttatcact tgtatatga 
      agaagagac catctgccg gtacagata ttacaaaaa 
      aagatgttg aaaaaccac cgcaaggct tcgtcgatg 
      ttgatttaa acgccaagg ctcctgccc atcatgacg 
      ttcctgctg tatacgaag acgctttgg cgaaccctg 
      ctttcgaag acaccgaat ttacggaag aaacgaatc 
      aagagaaaa acaaagacg tcaatccca acacgagct 
      aatcgttat tttccagta tttgaacat atttcatgg 
      ctagtctag acatttgtt ctgtacttg acaaggtta 
      aagtagtga aaagtccaa ttatatcta gtgatttca 
      acggatttt ccaccgttt gttattatt attgtatgt 
      aatttaatt aacacaatg ttattgcaa gtcagcggt 
      tcaatcggg gctcatggt ttagggtta caacaacga 
      ctgcgaaag tgttttcgg cgaaattga aaccgttgc 
      ataagaaac gtttgtttt gacgtctcc ttccaggat 
      gcgaacata taattgtat aacaagcac gagtataaa 
      ttattaagt ttaaatatt tccataaaa ggaaactta 
      acgggtttt ggccggtcg actaactga ccttataaa 
      taacgtaca ttgctttta acattaaat ttttagttt 
      ccgaccgaa aaaccggtc tttcattat cttgtattt 
      gcttccgga cccaattga actggctga cctgtactt 
      agttcctaa ttaatattt cggtcgttt aaaccatat 
      taattattt ttgcgtcgt aggtaattt gtatattta 
      gttattcta atcacacac tttgtacgt aaagcaggg 
      tctgtcagt tgacagtgc tagcagaac agctcttaa 
      atctgtgat atagcgaat aattgtaaa aaaatgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY