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Gene information
General information
TC004709 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 909579..924728
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004709
    iBeetle screen iB_03819

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004709 RA
      MCQCPTHCK QKVDLVCGT DGKTYANRC QLRVASCKA 
      RLNTRVKHD GECEQNDPC RDKQCGFGA RCVVSPDGR 
      NASCVCPDK CPSYGDHTT SRPVCGSDG IDYRNQCEL 
      QKAACTSNT NITIKFLGK CDPCAGVEC AEPEVCQLD 
      EHRNPVCRC GDSCPLEFT PVCGSDGKT YSNECTLRQ 
      EACRARKNL HIIYRGKCS SGINPCMSV RCTLGEECA 
      INKFGIARC QCPPSCEPI MRPVCSKDG RTFPSECEL 
      KRTACLTRT TIEISYSGV CGEKGPCSE YQCQNGATC 
      VERFGVAHC ECPVCPAEF EPVCGSDGI SYGNECKLR 
      LEACKHPRN ITVLYDGPC NGCETKKCD FYSVCERDS 
      ATEGRCVCP KSCTDPELN DGTVCGTDG VTYANECEL 
      RMTSCKMKQ FILVAYKGN CDLCQGVEC KYGGRCEAG 
      KCVCPTNCE GSGDEPVCA SNMMTFPNE CELQKAMCL 
      QPTNSPPLS VVFYGDCRE KFPVAGSLS TTLSPFTAQ 
      TNNIGPGTD LDTVGPENT IPDLSTVEK EACRDIHCD 
      FEATCELGP DNFPRCTCQ FDCASAALV SASKPVCAS 
      DLQIYPSLC AMKMEACQR QEELRLRPL DLCQGMEVK 
      PCNGEKPLT DPLTGKELD CGSGPKRQD CPSGSYCHQ 
      TTRFSRCCP KDQGLLEQK SCEDSWHGC CPDGKTAAQ 
      GPNHAGCPS LCGCNKLGS YSDTCDPET QQCRCRPGV 
      GGAKCDRCE PGYWGLPKI SSGYHGCIP CGCSTFGSV 
      RDDCEQMTG RCVCNPGVQ GQKCTVCTS HDKVLGPNG 
      CVPADLTTS PPTTCKELT CYFGATCVE RGGFAICEC 
      HTECPQEND AQVVCGSDG QTYASACEL RQVACRTQK 
      DIVVQAFGT CKDDMFPST DWPIRRYTP LQFTQPDDS 
      NSPLSKSTR HLLVPDPRY YYGSSGSIM RPVQGVSYP 
      AETDNKAAF EINVSATGP NAIYNTRGG NYAPAYRPT 
      PATVRVITA LLGDLCSDN SDCMIMYSH CVSGACTCL 
      PNHSESSDR QECIADPNP TDEYRACSS SPCHHGSTC 
      VDLPAATFT CVCETNFTG SLCETEVIH KQYNTPAFH 
      GRSYVKLKP LKAYHKLSI EVEFKTHSH DGLLLYNQQ 
      KPDGLGDFV SLAIVNGFV EFKYNLGNG HVLIRSVDK 
      IQLGVFHRV VIKRYHRDG ILKLDEGED VAGQAKGTL 
      KALDLLEDT YVGFLPSNY TRVYENIGT AKGFRGCIR 
      KLKIGRRPI ELHVNRDDW VLGAEAVSE CGDSPCSAA 
      PCANDAKCA LITPETYRC ECGPHYRGE FCEKPIEPC 
      RSNPCKFGS TCTPLSPDA YICECPPGR TGTRCEMED 
      KYMGIVNPE FSGSSYIRL PRLEGVRKT FSIEVYFMP 
      KAANGLILY NGQLKNGRG DFISLNLAR GHLQFRFNL 
      GSGIANLTT KETVNIGKW HWARIFRDG REGILQLDN 
      SSIVRGYSG TPLTELNLE LPFYIGSLS EWDEVHRLS 
      GASKGYKGV IQRILLNGK PLPISAKLP DCSFDSNRN 
      GCAFNVGSY DGLPCPISK NPCLNNGLC VPELDQFIC 
      KCPSNTKGK YCEISQEET PAIKFNGAT FLQFRNRGY 
      RSHNLTISD EYPDNEEDD IDNIEEDVV DNNTYDAYD 
      DEEYYFDFY DTARRKPER GNRYEIKLR TFASDALLL 
      WRSKSRSIR EDYFSIAVV NGYPEISYN LGKQDVFWA 
      IRSKTKIDD GKWHTIQVR RRKRVGFIS IDGEPPIKG 
      LSKYGAISL RTNSKLWIG GTANLPQGL PSAYYKGFE 
      GCIQHILVN AKPLDMVSN NELNKVNFC HDNEI
    CDS sequence
    • >TC004709 RA
      atgtgccaa tgtccaacc cattgcaag cagaaggtc 
      gacctcgtc tgcggaact gatggaaag acttacgct 
      aatcgctgt caacttcgt gtggcatct tgcaaagct 
      cgacttaac accagggtt aaacatgat ggggaatgc 
      gaacaaaac gatccatgt agagacaag caatgcggt 
      tttggggct cgatgtgtg gtttcccct gatggccgc 
      aatgccagc tgtgtctgt ccggataaa tgccccagc 
      tatggagat cataccacc tcgcgtcca gtctgtgga 
      agcgacggg atcgactat cgaaaccaa tgcgagctg 
      caaaaagcc gcctgcact tccaacaca aacatcacc 
      atcaagttt ctcggaaag tgcgatcct tgtgccgga 
      gtggaatgt gcagaaccc gaagtatgc cagttggac 
      gaacatcgc aatcctgtt tgtagatgt ggggacagt 
      tgtccttta gaatttact ccggtttgc ggatcggac 
      ggaaaaacc tactccaat gagtgcacg ctgcggcag 
      gaagcctgc agggcccgg aaaaacctc catatcata 
      tatcgcgga aaatgcagc tcaggcata aacccgtgc 
      atgagcgtg cggtgcacc ttaggggag gaatgtgcg 
      ataaataaa ttcggcatc gcccgctgt caatgcccg 
      ccgtcttgc gagcctatc atgaggccc gtttgttcg 
      aaagacggc cgaactttc ccatctgaa tgcgaattg 
      aaaagaacg gcttgtctg actcggacc actattgag 
      atatcttac agtggcgtt tgcggggag aaaggcccg 
      tgtagcgag taccagtgt caaaatgga gcgacctgc 
      gtcgaacga ttcggtgtt gcccactgc gagtgtccc 
      gtctgtccg gccgaattc gaacccgtc tgtggatcc 
      gacggtatt tcgtatggc aacgaatgt aagctaaga 
      ctggaagct tgcaagcat ccgcgcaat attacagtc 
      ctttacgac ggtccttgt aacggttgc gagactaaa 
      aaatgcgat ttttactcc gtttgcgag cgcgatagt 
      gcaactgag ggacgttgt gtttgcccc aagtcatgt 
      acggaccct gagctcaac gacggaacg gtttgtgga 
      acagatgga gtgacttac gcaaacgaa tgcgaactt 
      cgcatgacc tcttgcaaa atgaagcag ttcattttg 
      gtcgcgtat aagggaaac tgcgatcta tgtcaggga 
      gtggaatgt aaatacggg ggtagatgc gaagcgggc 
      aaatgcgta tgtccgacg aattgcgaa ggttccggc 
      gatgagccg gtgtgtgca tcaaacatg atgacgttc 
      cccaacgag tgcgaattg cagaaagcg atgtgttta 
      cagccgaca aattctccc cccttgagc gtagtgttc 
      tatggcgac tgtcgcgag aaattccca gtagcagga 
      tctctcagt acaactctt tcccccttc acggctcaa 
      actaacaac atcggcccg ggcacggat ctagacact 
      gtcggtccg gagaataca attccagac ttatcgacg 
      gtcgagaaa gaagcctgc cgtgatatt cattgcgat 
      ttcgaagcc acgtgcgag ttaggtcca gacaacttc 
      ccacgttgc acctgtcag ttcgattgt gccagtgcc 
      gcactcgtc tctgcctcg aaaccagta tgcgcctcc 
      gatttgcaa atctatcct tcgctttgc gctatgaag 
      atggaagcg tgtcaaagg caggaggag ttgcggttg 
      aggccactc gatctctgt caaggaatg gaggtaaaa 
      ccatgcaat ggcgaaaaa ccattaaca gaccccctc 
      acaggaaag gaattagat tgcggaagc ggcccgaaa 
      agacaagat tgcccttcc ggcagctac tgccatcaa 
      accacgcgg ttttcccgt tgttgtcca aaagaccag 
      ggcctttta gagcaaaag agctgcgag gatagctgg 
      cacgggtgt tgtcctgac ggaaaaacc gccgcccaa 
      ggccctaat cacgccgga tgtccttcc ctctgcggc 
      tgcaataaa ctcggctca tattccgac acgtgcgat 
      cccgaaacg caacagtgt cggtgtaga ccgggcgta 
      ggaggtgcc aaatgtgat cgatgcgaa cctggatat 
      tggggttta ccgaaaatc tcctccggc tatcacggt 
      tgcatcccg tgcggttgt tcgacgttc ggctccgtg 
      cgagacgat tgcgagcag atgacgggg agatgtgtg 
      tgcaacccg ggggtgcaa ggccaaaaa tgcacagta 
      tgcaccagc catgacaag gtcttagga ccgaacggg 
      tgcgtacca gcggatctg actacgtcg cccccgacg 
      acgtgcaaa gaattgact tgttatttt ggagcaaca 
      tgcgtcgaa cggggtgga tttgctata tgtgaatgt 
      cataccgaa tgccctcaa gaaaatgac gctcaagtc 
      gtgtgtgga agtgacgga cagacgtac gcgtcggcg 
      tgcgagttg cgacaagtc gcatgtcgg acgcaaaaa 
      gatatagtc gtccaagcg ttcgggacg tgtaaagat 
      gatatgttt ccaagtacg gattggcca attcgtcgt 
      tatactcct ctccagttt acacaaccc gacgattct 
      aattcgcct ttatcaaaa tcgacacga catttacta 
      gttcccgat cctcgttac tactacggg agcagcgga 
      tctattatg cgacccgtt caaggagtc tcttatccc 
      gctgaaaca gataataaa gccgcattc gaaataaac 
      gtatctgct acaggacct aacgccatt tacaatacc 
      cgcggtgga aactacgcc ccggcttac cgtccgacc 
      cccgcaacc gttcgcgta ataacagcc cttctcggg 
      gatctctgt tccgataac tccgattgc atgataatg 
      tatagccac tgcgtatca ggagcgtgc acttgtctt 
      ccgaatcac tctgaatcg tccgaccga caagagtgc 
      atagctgat ccgaatccg accgatgaa tatcgtgca 
      tgtagctca tccccctgt catcatggt agcacttgt 
      gttgatctt cccgccgcc acgttcact tgcgtttgc 
      gagacaaac tttacgggg tctctttgc gaaaccgaa 
      gtcattcac aaacaatac aacactcca gctttccat 
      gggcgttct tacgtcaaa ctcaaacct ctcaaagcc 
      taccacaaa cttagcatc gaagtcgag ttcaaaacg 
      cattcccac gacggattg ctgctctat aaccagcag 
      aaacctgac gggttgggc gatttcgtt tctcttgcc 
      atcgtcaac ggatttgtc gaattcaaa tacaatcta 
      ggcaacgga cacgttctt atccgttca gtcgacaaa 
      atacagctc ggcgtcttc caccgagtc gtcattaag 
      agatatcac agagacgga atactcaaa cttgatgaa 
      ggggaagac gtggccgga caggccaaa ggcacactc 
      aaagctctc gatttactt gaagataca tacgtcgga 
      tttctaccc tctaactac acaagagtt tacgaaaat 
      atcggcacc gcgaagggt tttcggggc tgcatcaga 
      aaattaaaa attggacga agaccgatc gagctgcac 
      gtcaacaga gacgattgg gtcttgggc gctgaggcg 
      gtatccgag tgcggtgac agcccctgc tcggcggcg 
      ccctgtgcc aacgacgcc aagtgcgcc ctaattaca 
      cctgaaacg taccgatgc gaatgcggc cctcattat 
      cggggcgag ttctgcgag aagccgatc gagccttgc 
      agatccaac ccttgcaaa ttcggatcg acgtgcacg 
      ccactcagc cccgacgct tacatctgc gaatgtcca 
      cctggacgg accggaact cggtgcgaa atggaagat 
      aaatatatg ggtatagta aatccggaa tttagtggg 
      tcaagttat atccgactt cctcgatta gagggtgtt 
      aggaagacg ttttcgatt gaggtgtat tttatgcca 
      aaggcagca aacggtctg atattgtat aacgggcaa 
      ctgaagaat ggccgcggt gattttatt tcgttgaac 
      ttagcacgt ggtcattta caatttaga ttcaatttg 
      ggaagcgga attgcgaat ttaacaact aaagagact 
      gtaaatatt gggaagtgg cattgggcc cgtattttc 
      cgagacgga cgagaagga attttacaa ttggataat 
      tctagcata gttcgtggt tattccggc acgccacta 
      acagaactt aatctggaa ttgccgttc tacatcgga 
      tctttatcg gagtgggat gaagtgcat cgtttatct 
      ggcgcatct aagggatat aagggcgta atacaaagg 
      attctccta aatggaaaa ccccttccc atttcggct 
      aaattgccg gattgctct tttgattct aatagaaac 
      ggatgcgca ttcaatgtc ggctcatat gacggatta 
      ccatgtccc atttccaaa aatccttgc ctaaataac 
      ggcctatgt gtaccagaa ctcgatcag tttatatgc 
      aaatgccct tctaacaca aagggaaaa tattgcgag 
      atatctcaa gaagaaacc cccgctatt aaattcaac 
      ggtgccact tttctccaa tttaggaac agaggatac 
      agaagtcac aatttaaca ataagtgat gaatatccc 
      gacaacgaa gaggacgat attgacaat attgaagaa 
      gacgtcgtc gataataac acttacgac gcttacgac 
      gacgaagaa tactacttc gatttttac gataccgct 
      agacgaaaa ccggaacgt ggcaacaga tacgaaatc 
      aaattgcgc acctttgcc agcgacgct cttctcttg 
      tggaggagt aaaagtaga agtataaga gaagactat 
      ttttctata gctgttgtt aatggttac cctgaaatt 
      agttataat ttaggcaag caagatgtc ttctgggca 
      atcagatcg aagacgaaa atcgacgac ggcaagtgg 
      catacaatc caagtaagg agacggaaa cgagtaggt 
      tttatatca atcgacggg gagccgcct attaagggc 
      ctctcgaaa tacggtgcc atttcactg agaacgaat 
      tctaaatta tggataggt ggtacggcg aatctgccc 
      caaggccta ccttcggcg tactacaaa ggcttcgaa 
      ggatgcatc caacatatt ttagttaac gcaaaaccc 
      ttagatatg gtttcaaat aatgaatta aataaagtt 
      aacttctgc cacgataac gaaatttaa
    mRNA sequence
    • >TC004709 RA
      ttggggggc ccagtgcat caccggccc cgacggcag 
      ggccatgtg ccaatgtcc aacccattg caagcagaa 
      ggtcgacct cgtctgcgg aactgatgg aaagactta 
      cgctaatcg ctgtcaact tcgtgtggc atcttgcaa 
      agctcgact taacaccag ggttaaaca tgatgggga 
      atgcgaaca aaacgatcc atgtagaga caagcaatg 
      cggttttgg ggctcgatg tgtggtttc ccctgatgg 
      ccgcaatgc cagctgtgt ctgtccgga taaatgccc 
      cagctatgg agatcatac cacctcgcg tccagtctg 
      tggaagcga cgggatcga ctatcgaaa ccaatgcga 
      gctgcaaaa agccgcctg cacttccaa cacaaacat 
      caccatcaa gtttctcgg aaagtgcga tccttgtgc 
      cggagtgga atgtgcaga acccgaagt atgccagtt 
      ggacgaaca tcgcaatcc tgtttgtag atgtgggga 
      cagttgtcc tttagaatt tactccggt ttgcggatc 
      ggacggaaa aacctactc caatgagtg cacgctgcg 
      gcaggaagc ctgcagggc ccggaaaaa cctccatat 
      catatatcg cggaaaatg cagctcagg cataaaccc 
      gtgcatgag cgtgcggtg caccttagg ggaggaatg 
      tgcgataaa taaattcgg catcgcccg ctgtcaatg 
      cccgccgtc ttgcgagcc tatcatgag gcccgtttg 
      ttcgaaaga cggccgaac tttcccatc tgaatgcga 
      attgaaaag aacggcttg tctgactcg gaccactat 
      tgagatatc ttacagtgg cgtttgcgg ggagaaagg 
      cccgtgtag cgagtacca gtgtcaaaa tggagcgac 
      ctgcgtcga acgattcgg tgttgccca ctgcgagtg 
      tcccgtctg tccggccga attcgaacc cgtctgtgg 
      atccgacgg tatttcgta tggcaacga atgtaagct 
      aagactgga agcttgcaa gcatccgcg caatattac 
      agtccttta cgacggtcc ttgtaacgg ttgcgagac 
      taaaaaatg cgattttta ctccgtttg cgagcgcga 
      tagtgcaac tgagggacg ttgtgtttg ccccaagtc 
      atgtacgga ccctgagct caacgacgg aacggtttg 
      tggaacaga tggagtgac ttacgcaaa cgaatgcga 
      acttcgcat gacctcttg caaaatgaa gcagttcat 
      tttggtcgc gtataaggg aaactgcga tctatgtca 
      gggagtgga atgtaaata cgggggtag atgcgaagc 
      gggcaaatg cgtatgtcc gacgaattg cgaaggttc 
      cggcgatga gccggtgtg tgcatcaaa catgatgac 
      gttccccaa cgagtgcga attgcagaa agcgatgtg 
      tttacagcc gacaaattc tcccccctt gagcgtagt 
      gttctatgg cgactgtcg cgagaaatt cccagtagc 
      aggatctct cagtacaac tctttcccc cttcacggc 
      tcaaactaa caacatcgg cccgggcac ggatctaga 
      cactgtcgg tccggagaa tacaattcc agacttatc 
      gacggtcga gaaagaagc ctgccgtga tattcattg 
      cgatttcga agccacgtg cgagttagg tccagacaa 
      cttcccacg ttgcacctg tcagttcga ttgtgccag 
      tgccgcact cgtctctgc ctcgaaacc agtatgcgc 
      ctccgattt gcaaatcta tccttcgct ttgcgctat 
      gaagatgga agcgtgtca aaggcagga ggagttgcg 
      gttgaggcc actcgatct ctgtcaagg aatggaggt 
      aaaaccatg caatggcga aaaaccatt aacagaccc 
      cctcacagg aaaggaatt agattgcgg aagcggccc 
      gaaaagaca agattgccc ttccggcag ctactgcca 
      tcaaaccac gcggttttc ccgttgttg tccaaaaga 
      ccagggcct tttagagca aaagagctg cgaggatag 
      ctggcacgg gtgttgtcc tgacggaaa aaccgccgc 
      ccaaggccc taatcacgc cggatgtcc ttccctctg 
      cggctgcaa taaactcgg ctcatattc cgacacgtg 
      cgatcccga aacgcaaca gtgtcggtg tagaccggg 
      cgtaggagg tgccaaatg tgatcgatg cgaacctgg 
      atattgggg tttaccgaa aatctcctc cggctatca 
      cggttgcat cccgtgcgg ttgttcgac gttcggctc 
      cgtgcgaga cgattgcga gcagatgac ggggagatg 
      tgtgtgcaa cccgggggt gcaaggcca aaaatgcac 
      agtatgcac cagccatga caaggtctt aggaccgaa 
      cgggtgcgt accagcgga tctgactac gtcgccccc 
      gacgacgtg caaagaatt gacttgtta ttttggagc 
      aacatgcgt cgaacgggg tggatttgc tatatgtga 
      atgtcatac cgaatgccc tcaagaaaa tgacgctca 
      agtcgtgtg tggaagtga cggacagac gtacgcgtc 
      ggcgtgcga gttgcgaca agtcgcatg tcggacgca 
      aaaagatat agtcgtcca agcgttcgg gacgtgtaa 
      agatgatat gtttccaag tacggattg gccaattcg 
      tcgttatac tcctctcca gtttacaca acccgacga 
      ttctaattc gcctttatc aaaatcgac acgacattt 
      actagttcc cgatcctcg ttactacta cgggagcag 
      cggatctat tatgcgacc cgttcaagg agtctctta 
      tcccgctga aacagataa taaagccgc attcgaaat 
      aaacgtatc tgctacagg acctaacgc catttacaa 
      tacccgcgg tggaaacta cgccccggc ttaccgtcc 
      gacccccgc aaccgttcg cgtaataac agcccttct 
      cggggatct ctgttccga taactccga ttgcatgat 
      aatgtatag ccactgcgt atcaggagc gtgcacttg 
      tcttccgaa tcactctga atcgtccga ccgacaaga 
      gtgcatagc tgatccgaa tccgaccga tgaatatcg 
      tgcatgtag ctcatcccc ctgtcatca tggtagcac 
      ttgtgttga tcttcccgc cgccacgtt cacttgcgt 
      ttgcgagac aaactttac ggggtctct ttgcgaaac 
      cgaagtcat tcacaaaca atacaacac tccagcttt 
      ccatgggcg ttcttacgt caaactcaa acctctcaa 
      agcctacca caaacttag catcgaagt cgagttcaa 
      aacgcattc ccacgacgg attgctgct ctataacca 
      gcagaaacc tgacgggtt gggcgattt cgtttctct 
      tgccatcgt caacggatt tgtcgaatt caaatacaa 
      tctaggcaa cggacacgt tcttatccg ttcagtcga 
      caaaataca gctcggcgt cttccaccg agtcgtcat 
      taagagata tcacagaga cggaatact caaacttga 
      tgaagggga agacgtggc cggacaggc caaaggcac 
      actcaaagc tctcgattt acttgaaga tacatacgt 
      cggatttct accctctaa ctacacaag agtttacga 
      aaatatcgg caccgcgaa gggttttcg gggctgcat 
      cagaaaatt aaaaattgg acgaagacc gatcgagct 
      gcacgtcaa cagagacga ttgggtctt gggcgctga 
      ggcggtatc cgagtgcgg tgacagccc ctgctcggc 
      ggcgccctg tgccaacga cgccaagtg cgccctaat 
      tacacctga aacgtaccg atgcgaatg cggccctca 
      ttatcgggg cgagttctg cgagaagcc gatcgagcc 
      ttgcagatc caacccttg caaattcgg atcgacgtg 
      cacgccact cagccccga cgcttacat ctgcgaatg 
      tccacctgg acggaccgg aactcggtg cgaaatgga 
      agataaata tatgggtat agtaaatcc ggaatttag 
      tgggtcaag ttatatccg acttcctcg attagaggg 
      tgttaggaa gacgttttc gattgaggt gtattttat 
      gccaaaggc agcaaacgg tctgatatt gtataacgg 
      gcaactgaa gaatggccg cggtgattt tatttcgtt 
      gaacttagc acgtggtca tttacaatt tagattcaa 
      tttgggaag cggaattgc gaatttaac aactaaaga 
      gactgtaaa tattgggaa gtggcattg ggcccgtat 
      tttccgaga cggacgaga aggaatttt acaattgga 
      taattctag catagttcg tggttattc cggcacgcc 
      actaacaga acttaatct ggaattgcc gttctacat 
      cggatcttt atcggagtg ggatgaagt gcatcgttt 
      atctggcgc atctaaggg atataaggg cgtaataca 
      aaggattct cctaaatgg aaaacccct tcccatttc 
      ggctaaatt gccggattg ctcttttga ttctaatag 
      aaacggatg cgcattcaa tgtcggctc atatgacgg 
      attaccatg tcccatttc caaaaatcc ttgcctaaa 
      taacggcct atgtgtacc agaactcga tcagtttat 
      atgcaaatg cccttctaa cacaaaggg aaaatattg 
      cgagatatc tcaagaaga aacccccgc tattaaatt 
      caacggtgc cacttttct ccaatttag gaacagagg 
      atacagaag tcacaattt aacaataag tgatgaata 
      tcccgacaa cgaagagga cgatattga caatattga 
      agaagacgt cgtcgataa taacactta cgacgctta 
      cgacgacga agaatacta cttcgattt ttacgatac 
      cgctagacg aaaaccgga acgtggcaa cagatacga 
      aatcaaatt gcgcacctt tgccagcga cgctcttct 
      cttgtggag gagtaaaag tagaagtat aagagaaga 
      ctatttttc tatagctgt tgttaatgg ttaccctga 
      aattagtta taatttagg caagcaaga tgtcttctg 
      ggcaatcag atcgaagac gaaaatcga cgacggcaa 
      gtggcatac aatccaagt aaggagacg gaaacgagt 
      aggttttat atcaatcga cggggagcc gcctattaa 
      gggcctctc gaaatacgg tgccatttc actgagaac 
      gaattctaa attatggat aggtggtac ggcgaatct 
      gccccaagg cctaccttc ggcgtacta caaaggctt 
      cgaaggatg catccaaca tattttagt taacgcaaa 
      acccttaga tatggtttc aaataatga attaaataa 
      agttaactt ctgccacga taacgaaat ttaaatgat 
      gtaaattat gttgcacgg cgcaatatt aaataattt 
      aaaactgtg agactgata tattgtact cctcaattt 
      tccatttac agaatttag cttagagat gatgttgta 
      tataaatgt actgttaat aaattacag cagttttat 
      gtagcaact ttgaaacat tggctgcca gtacaataa 
      ttacctact aacatatca aacgggaag tttttactt 
      aatttttca aatgtcttc agttgtaag cattagaga 
      ctggaaaca ttggagcca attaaaagt gtacaaata 
      ttttttgtt cctagtagt tgtaatttg gattaaaac 
      atatctttc gaagttggc agtcaatgt attaaatag 
      acacggtaa ataggagtg tttttacaa agctgtttg 
      tatctagtc ttgtattta tttccgaat taggtcttg 
      gattttgta aaggacgac gtaaaacct gccatttct 
      aagttctcg tggactgac actttatcg atagttgag 
      gctttaatt tgatactta taatgaatg cttaaataa 
      gaaatgtgg actaaaatt gtttgtatg gccaatgtt 
      gaaatttat tatcttgaa aatgtttag atgttaccc 
      gattccagt aacaactgt gtgaacttc atatctgta 
      actactgat taagtcgaa ttacttgtt ttctggcaa 
      taattttta tggtacata agaggagtt aatatgctt 
      gtatgttat gtaaatagc caatacgaa atgatagtt 
      attttaggt aatgttaat ttatagtaa aaattttat 
      tacgtaatg aatagattg atgagctaa attaattat 
      aatgataag ttttgttta gtatactga ttgtttcta 
      ttgtagacc tctgtacta taaatgcta caaaatata 
      tttttttcc tacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY