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Gene information
General information
TC004706 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 718218..754501
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004706
      iBeetle screen iB_03817
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      remarks: bad quality of preparation,
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection



      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      Adult morphology
      elytra not closed - Number of animals showing the phenotype: 2


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004706 RA
        MALKGCRPR ATILRLFLL ATLWLHSHA 
        SALARRQIS AHPVFLPRC KYDEHIIKS 
        EDKLQKQID LANFVFTGK VTSDIRFLD 
        NRTIVFSVY VRRYFKNNA GLSDNQEVR 
        VLKTLYDGE GVKCRQVLR YRYTAIFIG 
        RKPQKLDAD VQLTINPVP VTLTNLDRV 
        TAATKAKFK VSFFLNDKN REFLDSSLA 
        SQQHARKQV VRSDLLVDC DALSDLVQC 
        PIES
      • >TC004706 RB
        MALKGCRPR ATILRLFLL ATLWLHSHA 
        SALARRQIS AHPVFLPRC KYDEHIIKS 
        EDKLQKQID LANFVFTGK VTSDIRFLD 
        NRTIVFSVY VRRYFKNNA GLSDNQEVR 
        VLKTLYDGE GVKCRQVLR YRYTAIFIG 
        RKPQKLDAD VQLTINPVP VTLTNLDRV 
        TAATKDKNR EFLDSSLAS QQHARKQVV 
        RSDLLVDCD ALSDLVQCP IES
      CDS sequence
      • >TC004706 RA
        atggcactg aaagggtgt aggccacgt gccacaatc 
        ctgcgactg ttcctcctc gccaccctc tggctgcac 
        agccatgcc tcggcgctg gcacgccgc caaatttca 
        gcgcacccc gtcttcctg ccccgatgc aaatacgac 
        gagcatata attaagagc gaggataaa ctccaaaaa 
        cagatcgat ctcgccaat ttcgtcttc acggggaaa 
        gtgacgagt gacatacga ttcctcgac aaccgcact 
        attgtcttc agcgtttat gtcaggcgc tatttcaaa 
        aacaacgcc ggcctcagc gacaatcag gaagtgaga 
        gtgctcaaa acattatat gacggcgaa ggagttaag 
        tgtcgacaa gttttaaga tataggtac acggcgatc 
        tttattgga cgaaaaccg caaaagctc gacgcggat 
        gtgcaactc actattaac cccgtacca gttacttta 
        accaatctt gacagggtt accgccgcc actaaagcc 
        aaattcaaa gtctctttt ttccttaat gataaaaat 
        agggagttc ttggacagt agtttggcc agtcagcag 
        catgcaaga aaacaagtg gttcgatcg gatttgttg 
        gtcgattgc gatgcatta agtgatttg gttcagtgt 
        ccgattgag tcgtaa
      • >TC004706 RB
        atggcactg aaagggtgt aggccacgt gccacaatc 
        ctgcgactg ttcctcctc gccaccctc tggctgcac 
        agccatgcc tcggcgctg gcacgccgc caaatttca 
        gcgcacccc gtcttcctg ccccgatgc aaatacgac 
        gagcatata attaagagc gaggataaa ctccaaaaa 
        cagatcgat ctcgccaat ttcgtcttc acggggaaa 
        gtgacgagt gacatacga ttcctcgac aaccgcact 
        attgtcttc agcgtttat gtcaggcgc tatttcaaa 
        aacaacgcc ggcctcagc gacaatcag gaagtgaga 
        gtgctcaaa acattatat gacggcgaa ggagttaag 
        tgtcgacaa gttttaaga tataggtac acggcgatc 
        tttattgga cgaaaaccg caaaagctc gacgcggat 
        gtgcaactc actattaac cccgtacca gttacttta 
        accaatctt gacagggtt accgccgcc actaaagat 
        aaaaatagg gagttcttg gacagtagt ttggccagt 
        cagcagcat gcaagaaaa caagtggtt cgatcggat 
        ttgttggtc gattgcgat gcattaagt gatttggtt 
        cagtgtccg attgagtcg taa
      mRNA sequence
      • >TC004706 RA
        tagctgaag gaaggagcg gtaccgtgc gtggtagag 
        tgttgagcc agtcatgcc gcgtcttta gtcgtgcgt 
        gtttcttgc catggcact gaaagggtg taggccacg 
        tgccacaat cctgcgact gttcctcct cgccaccct 
        ctggctgca cagccatgc ctcggcgct ggcacgccg 
        ccaaatttc agcgcaccc cgtcttcct gccccgatg 
        caaatacga cgagcatat aattaagag cgaggataa 
        actccaaaa acagatcga tctcgccaa tttcgtctt 
        cacggggaa agtgacgag tgacatacg attcctcga 
        caaccgcac tattgtctt cagcgttta tgtcaggcg 
        ctatttcaa aaacaacgc cggcctcag cgacaatca 
        ggaagtgag agtgctcaa aacattata tgacggcga 
        aggagttaa gtgtcgaca agttttaag atataggta 
        cacggcgat ctttattgg acgaaaacc gcaaaagct 
        cgacgcgga tgtgcaact cactattaa ccccgtacc 
        agttacttt aaccaatct tgacagggt taccgccgc 
        cactaaagc caaattcaa agtctcttt tttccttaa 
        tgataaaaa tagggagtt cttggacag tagtttggc 
        cagtcagca gcatgcaag aaaacaagt ggttcgatc 
        ggatttgtt ggtcgattg cgatgcatt aagtgattt 
        ggttcagtg tccgattga gtcgtaacg gaataaacc 
        taagttgaa ttg
      • >TC004706 RB
        tagctgaag gaaggagcg gtaccgtgc gtggtagag 
        tgttgagcc agtcatgcc gcgtcttta gtcgtgcgt 
        gtttcttgc catggcact gaaagggtg taggccacg 
        tgccacaat cctgcgact gttcctcct cgccaccct 
        ctggctgca cagccatgc ctcggcgct ggcacgccg 
        ccaaatttc agcgcaccc cgtcttcct gccccgatg 
        caaatacga cgagcatat aattaagag cgaggataa 
        actccaaaa acagatcga tctcgccaa tttcgtctt 
        cacggggaa agtgacgag tgacatacg attcctcga 
        caaccgcac tattgtctt cagcgttta tgtcaggcg 
        ctatttcaa aaacaacgc cggcctcag cgacaatca 
        ggaagtgag agtgctcaa aacattata tgacggcga 
        aggagttaa gtgtcgaca agttttaag atataggta 
        cacggcgat ctttattgg acgaaaacc gcaaaagct 
        cgacgcgga tgtgcaact cactattaa ccccgtacc 
        agttacttt aaccaatct tgacagggt taccgccgc 
        cactaaaga taaaaatag ggagttctt ggacagtag 
        tttggccag tcagcagca tgcaagaaa acaagtggt 
        tcgatcgga tttgttggt cgattgcga tgcattaag 
        tgatttggt tcagtgtcc gattgagtc gtaacggaa 
        taaacctaa gttgaattg