Status
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Gene information
General information
TC031468
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004693,TC004694
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 111282..116449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031468
    iBeetle screen iB_06545

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Around 30 larvae on slide.

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs podomer partially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Pupal morphology
    female genital lobes potentially shortened - Number of animals showing the phenotype: 8

    iBeetle screen iB_03814

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031468 RA
      MGDKIKNIP EQKLKAFSI GTMGKRSLS KKELEEQRK 
      KEEERAAAH VFEEFVATF QESPISSSS KVWVKAGTY 
      DAGARKEDT KDKGKLYKP QSRLAPTEL MASTEKAQF 
      YAKLISDKK PERLGKKKQ HTKSNLEAF KEELRQIQE 
      EREERHKYK GQQLMEIFG RYGPLASIK IMWPRSDEE 
      KARGRNCGF VAYMSRKDG ERALRNLNG KEILGYEMK 
      LGWGKSVII PPHPIYIPP ALLELSFPP PPSGLPFNA 
      QPCARDKDV LPKSAEELN EILGRSVVK VVIPTDRNL 
      LMLIHRMVE FVVREGPML EAMIMNREI HNPNYRFLF 
      ENQSPAHIY YRWKLYSIM HGDSQKEWS VKEFRLFKN 
      GSIWKPPLM NSYTAGMPD ELVNDDEGK ESTKGSLSN 
      TQRDRLEDL IRSLTPERS KIGEAMVFC IEHSDAAEE 
      VVECITESL SNSSTLITK KIARLYLVS DILHNCGVK 
      VNNASYYRK AFESKLVDI MTEVKKSYD KLEGRLQAE 
      GCKLRVLRI LQALDDSIY HKDYVNKLR TVFLGIKEP 
      VREPKEDPA VESNIDIDG DPLQDQPET DDDVPMDGA 
      TLLKSAMKR YKNTTISND DIDGKEIVD DPNDVVLNQ 
      KKPPAPLSG FIMSKWETI DPEQVEAQA MTTSKWDLL 
      GSSADPSQD GSSNDKDDS NDYTDTRNM TEERRNKLR 
      EIEVKAVQY QDELESGQR ALKTGWTLP QQVEHYRRK 
      LLKKSEKER KEKDPKSDK HKKDDRKED SSEDELYYK 
      ELKSRKSKK LSRSSSSGS TSKHRSKSR SPPRKKAFR 
      GPSPPSPPH IRRQSPSSS RSRKSKDSL SPISSSNGK 
      YGSPARVAK SRRSPSPSR TKSNRRSTS PRNRRSPSP 
      RSRRGSTSP YRSRYHSPE SSRKHRHKH KY
    CDS sequence
    • >TC031468 RA
      atgggtgat aaaataaag aacattcct gagcaaaaa 
      ttaaaggct ttttcgatc ggcacgatg gggaaacgt 
      tcgttatct aaaaaagaa ttagaggaa caacggaaa 
      aaagaagag gaacgggct gcggctcat gtttttgag 
      gaatttgtc gctacgttc caagagtcc cctattagc 
      agctcgagt aaagtgtgg gtcaaagcg gggacttac 
      gatgcagga gcgcgaaag gaggatact aaagacaag 
      ggcaagttg tacaaaccg cagtcccga ctagccccc 
      actgaatta atggcatca acggaaaaa gctcagttt 
      tacgctaaa ttaatctca gataagaag cctgaacgg 
      ttagggaag aagaaacag cacactaag agcaaccta 
      gaggcgttt aaagaggaa ttaagacag attcaggag 
      gagcgggag gaaagacac aagtacaaa gggcaacag 
      ttgatggaa attttcggt cgttacggc ccccttgct 
      agtattaaa ataatgtgg ccccgctcg gacgaggag 
      aaggcccgg ggccgcaac tgcggcttc gtggcctac 
      atgagccga aaagacggc gaaagggcg cttcgtaac 
      ttaaacggc aaagaaatc ctaggttat gaaatgaaa 
      cttggttgg ggaaaatcg gtaataatc cctccacat 
      cccatttac atccctccc gcgctcctg gaactgtcc 
      ttccctccg cctccctca ggactgccc ttcaacgcc 
      cagccctgc gcccgggat aaggacgtt ctgcccaaa 
      tctgcagag gaactgaac gagattttg gggcgcagt 
      gttgtcaaa gttgtgatt ccgaccgat agaaatctg 
      ttgatgttg atacacaga atggtcgag tttgtggtt 
      cgggagggg cccatgttg gaagctatg attatgaac 
      cgggagata cacaaccct aattacagg tttcttttc 
      gaaaatcaa agcccggct catatctac taccggtgg 
      aaactctac tccatcatg cacggggat tcacagaaa 
      gagtggagc gtcaaagag tttcgactt ttcaaaaac 
      ggttcaatt tggaagccc cctctgatg aattcgtac 
      actgccggg atgcccgat gaactagtg aacgacgac 
      gagggtaaa gagagtacg aaaggctcc ctctctaac 
      acgcaacga gaccgcttg gaagacctg atacggtcc 
      ctaaccccc gaacggagc aaaatcggc gaagctatg 
      gtcttctgc attgaacac agtgacgct gctgaggaa 
      gtggtcgag tgtatcacc gaatcacta tcaaactcc 
      tcaaccctc atcacgaaa aaaatcgcc agactttac 
      ctcgtgtcg gatattttg cacaactgc ggcgtcaaa 
      gtcaacaat gcgtcttac taccgcaag gcgtttgag 
      tcaaaactt gtggatata atgaccgaa gtgaagaag 
      tcctacgac aagttggaa gggcggttg caggccgag 
      ggttgcaag ctgcgcgtt ttgcggata ttgcaggcg 
      ttggacgac tcgatttac cacaaggat tacgtgaac 
      aaattacga accgtgttt ttgggtatc aaggaaccg 
      gtcagggaa cctaaagag gaccccgct gttgagtca 
      aatattgat atagacggc gatccgttg caagaccag 
      cccgaaacc gacgatgat gtgccaatg gatggagca 
      acgttgctg aaaagtgca atgaaacgg tacaagaac 
      accacgatt tcgaatgat gatattgac gggaaggaa 
      attgtcgat gatccgaac gatgttgtg cttaatcag 
      aagaaacca cccgcgcct ttgtccggt tttattatg 
      tcaaagtgg gaaacaatc gacccggaa caagtcgaa 
      gcgcaagcg atgaccaca agcaagtgg gacttgctg 
      ggaagtagc gctgatccc agtcaggat ggttcctca 
      aacgacaag gacgattcc aatgattat accgacacg 
      agaaatatg acggaagaa agaaggaac aaattgcga 
      gaaattgaa gtgaaagcc gtacagtac caagacgaa 
      ttagagtca ggccaaaga gctttgaaa accgggtgg 
      accttacca caacaagtg gaacactat cgaagaaag 
      ttactaaag aagagcgag aaagaaagg aaggagaaa 
      gacccgaaa agcgacaaa cacaaaaaa gatgaccgg 
      aaagaggac agctcagag gacgaatta tattacaaa 
      gagcttaaa tctaggaaa tcaaaaaaa ctttcgcgc 
      agctcgtca agcggttcc acgtccaaa cacaggtcg 
      aaaagtcgt tcgcctccg aggaagaaa gcttttcga 
      gggccgagt ccgcccagc ccccctcac atccggcgt 
      cagtcgccc tcctcgtcc cgatcccgc aagtccaaa 
      gattccctc tctccgatt agttcctca aatggtaaa 
      tatggttca ccagctcgt gtggcgaaa agtcggcga 
      tcaccgtca ccttcacgt acaaaaagt aatcgtaga 
      tcgacttct ccgcgaaat cggagatct ccgtctccg 
      agaagtcgt cgtgggagc acgtcaccg taccggtcc 
      aggtatcac tcgccggag tcttcaaga aaacatcga 
      cacaaacac aaatactga
    mRNA sequence
    • >TC031468 RA
      aacgcaaac tccctaaaa ccaccagtg tattattat 
      tttttcata tcaggaaat taaacaatt atattatga 
      ctattatta ggtttcctt caatattaa ttagctaaa 
      accgttttg tgaagtgtt tggtgggaa ttgcgtcca 
      aaatgggtg ataaaataa agaacattc ctgagcaaa 
      aattaaagg ctttttcga tcggcacga tggggaaac 
      gttcgttat ctaaaaaag aattagagg aacaacgga 
      aaaaagaag aggaacggg ctgcggctc atgtttttg 
      aggaatttg tcgctacgt tccaagagt cccctatta 
      gcagctcga gtaaagtgt gggtcaaag cggggactt 
      acgatgcag gagcgcgaa aggaggata ctaaagaca 
      agggcaagt tgtacaaac cgcagtccc gactagccc 
      ccactgaat taatggcat caacggaaa aagctcagt 
      tttacgcta aattaatct cagataaga agcctgaac 
      ggttaggga agaagaaac agcacacta agagcaacc 
      tagaggcgt ttaaagagg aattaagac agattcagg 
      aggagcggg aggaaagac acaagtaca aagggcaac 
      agttgatgg aaattttcg gtcgttacg gcccccttg 
      ctagtatta aaataatgt ggccccgct cggacgagg 
      agaaggccc ggggccgca actgcggct tcgtggcct 
      acatgagcc gaaaagacg gcgaaaggg cgcttcgta 
      acttaaacg gcaaagaaa tcctaggtt atgaaatga 
      aacttggtt ggggaaaat cggtaataa tccctccac 
      atcccattt acatccctc ccgcgctcc tggaactgt 
      ccttccctc cgcctccct caggactgc ccttcaacg 
      cccagccct gcgcccggg ataaggacg ttctgccca 
      aatctgcag aggaactga acgagattt tggggcgca 
      gtgttgtca aagttgtga ttccgaccg atagaaatc 
      tgttgatgt tgatacaca gaatggtcg agtttgtgg 
      ttcgggagg ggcccatgt tggaagcta tgattatga 
      accgggaga tacacaacc ctaattaca ggtttcttt 
      tcgaaaatc aaagcccgg ctcatatct actaccggt 
      ggaaactct actccatca tgcacgggg attcacaga 
      aagagtgga gcgtcaaag agtttcgac ttttcaaaa 
      acggttcaa tttggaagc cccctctga tgaattcgt 
      acactgccg ggatgcccg atgaactag tgaacgacg 
      acgagggta aagagagta cgaaaggct ccctctcta 
      acacgcaac gagaccgct tggaagacc tgatacggt 
      ccctaaccc ccgaacgga gcaaaatcg gcgaagcta 
      tggtcttct gcattgaac acagtgacg ctgctgagg 
      aagtggtcg agtgtatca ccgaatcac tatcaaact 
      cctcaaccc tcatcacga aaaaaatcg ccagacttt 
      acctcgtgt cggatattt tgcacaact gcggcgtca 
      aagtcaaca atgcgtctt actaccgca aggcgtttg 
      agtcaaaac ttgtggata taatgaccg aagtgaaga 
      agtcctacg acaagttgg aagggcggt tgcaggccg 
      agggttgca agctgcgcg ttttgcgga tattgcagg 
      cgttggacg actcgattt accacaagg attacgtga 
      acaaattac gaaccgtgt ttttgggta tcaaggaac 
      cggtcaggg aacctaaag aggaccccg ctgttgagt 
      caaatattg atatagacg gcgatccgt tgcaagacc 
      agcccgaaa ccgacgatg atgtgccaa tggatggag 
      caacgttgc tgaaaagtg caatgaaac ggtacaaga 
      acaccacga tttcgaatg atgatattg acgggaagg 
      aaattgtcg atgatccga acgatgttg tgcttaatc 
      agaagaaac cacccgcgc ctttgtccg gttttatta 
      tgtcaaagt gggaaacaa tcgacccgg aacaagtcg 
      aagcgcaag cgatgacca caagcaagt gggacttgc 
      tgggaagta gcgctgatc ccagtcagg atggttcct 
      caaacgaca aggacgatt ccaatgatt ataccgaca 
      cgagaaata tgacggaag aaagaagga acaaattgc 
      gagaaattg aagtgaaag ccgtacagt accaagacg 
      aattagagt caggccaaa gagctttga aaaccgggt 
      ggaccttac cacaacaag tggaacact atcgaagaa 
      agttactaa agaagagcg agaaagaaa ggaaggaga 
      aagacccga aaagcgaca aacacaaaa aagatgacc 
      ggaaagagg acagctcag aggacgaat tatattaca 
      aagagctta aatctagga aatcaaaaa aactttcgc 
      gcagctcgt caagcggtt ccacgtcca aacacaggt 
      cgaaaagtc gttcgcctc cgaggaaga aagcttttc 
      gagggccga gtccgccca gcccccctc acatccggc 
      gtcagtcgc cctcctcgt cccgatccc gcaagtcca 
      aagattccc tctctccga ttagttcct caaatggta 
      aatatggtt caccagctc gtgtggcga aaagtcggc 
      gatcaccgt caccttcac gtacaaaaa gtaatcgta 
      gatcgactt ctccgcgaa atcggagat ctccgtctc 
      cgagaagtc gtcgtggga gcacgtcac cgtaccggt 
      ccaggtatc actcgccgg agtcttcaa gaaaacatc 
      gacacaaac acaaatact gagagcgat attttatta 
      cgaataatt gaggtattg aacacacgc tcaataata 
      ttgaaagga gtttattat gttgtcttc aattacttt 
      ttttctttt acaaataca atggatctt tacgccttc 
      gaagttagc gttgttttg tttttttta tgtaaatcc 
      aacactaaa taaaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY