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Gene information
General information
TC034513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 230196..246370
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034513
    iBeetle screen iB_03813

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034513 RA
      MSNGNSHLA NGSMWAEGK PSNNNGPSN GSTRLGMED 
      IPEEQTALT NQAEVYRSQ CFASEGDFP SDSDTECGL 
      GPFAPHWLQ MFASKQAFL IVFCIAWVL QGMYHTYFV 
      SAITTIEKL FQIQSKITG IIMSATEIG QIGSSLLLT 
      YYGGQGHRP KWIAWGWFC SRQLISAND LTGITKDPN 
      VCKLPSAID ALEPSAHWG NTTLPVTAS GVGVSNILQ 
      TCHEELDTE HRIQPSVTK IVLAIFFIS LLGVGMGQT 
      AVYTLGIPY IDDNVASKE SPLYFAITI GVRILGPAL 
      GFIVGSLCT SVYADLSVD PKIDTTDPR WVGAWYLGL 
      VLISGLLML ASFAMFAFP KRLKTAKPT PRIHQPGRK 
      HPSIRDFPK TVKRLLKND ILMFRTASS VLHILPIAG 
      LYTFLPKYL ESQFRLPTP SANMISGVG GILVMGVGI 
      IISGVFILR VKPNARFVA AWIAFTAII YAVGMGILM 
      FIGCPMDDL AGIQTRHEF ATFDLNCNK STCECNQDK 
      FSPICGQDG KTYLSPCHA GCRNYTEKE GKIVEYSDC 
      LCVAHDDNV TISLPDSVL YGNATIGYC DVQCPNFTW 
      YIILFSIFV FIHSTSEVG SMLLILRCV DPRDKAMAL 
      GLIQFAIGL FGNVPCPIV YGAVVDSAC LAGSINFVD 
      EQVPLEEEL TSMTKKALT EPVSLPQ
    CDS sequence
    • >TC034513 RA
      atgtcgaac ggcaacagc cacctggcc aacggctcg 
      atgtgggcc gaaggcaag ccgagcaac aacaacggc 
      ccctccaac ggcagcacc cgcctgggc atggaggac 
      atccccgag gagcagacg gccctcacg aaccaggcc 
      gaggtgtac cgatcgcag tgcttcgcc agtgagggt 
      gatttcccg agcgacagc gacacggaa tgcggtctg 
      gggccgttc gcgccccac tggctccaa atgttcgcc 
      tcgaagcaa gccttcctc atagttttc tgcatcgcc 
      tgggtcctc cagggcatg tatcacacg tacttcgtc 
      agtgcgata accacaatc gaaaaactc tttcaaatt 
      cagtcgaaa atcaccggg attatcatg agtgccacc 
      gaaatcggc cagatcggc tcgtcgctg ctcctgacg 
      tactacggg gggcagggg caccgcccc aagtggatc 
      gcgtgggga tggttctgt tcgcgacag ttaattagc 
      gccaatgat ctcacgggg atcaccaag gacccgaac 
      gtttgcaaa ctccccagt gcaatagac gcgctggag 
      cccagcgcc cactggggc aacaccacc ctcccggtc 
      accgcgagc ggggtgggg gtctcaaat attttgcaa 
      acttgtcat gaggagctc gacacggag caccggatc 
      caaccctcg gtcacgaaa attgttctc gcgattttt 
      tttattagc ttgttgggg gtgggaatg gggcaaacg 
      gctgtgtac actttgggg attccctac atcgacgac 
      aacgtcgcc agcaaggag tcgccgctt tatttcgcg 
      ataacaatc ggcgtgcga attctgggt ccggctctg 
      ggcttcatc gtcggatcc ctctgtact agcgtgtac 
      gcggattta tcagtcgat cctaaaata gatacgaca 
      gatccaagg tgggttggg gcatggtac ttgggtttg 
      gttttaata tcagggcta ctaatgctg gcctctttc 
      gccatgttc gccttcccg aaacggctg aaaacggcg 
      aaaccgacg cccaggata caccaacct ggacgaaaa 
      catcctagc ataagagac ttccccaaa acggtcaag 
      cggctgctc aaaaacgat atcctaatg tttcggacc 
      gcaagcagc gtcttgcac attcttcca atcgctgga 
      ttgtacaca tttttgcca aagtattta gaatctcag 
      ttcaggcta cccactcct tcggccaat atgatatca 
      ggagttggg ggcattttg gtgatgggc gtcggcata 
      attataagc ggggtcttc atccttcga gtcaaacct 
      aacgccagg tttgtcgca gcctggatc gcattcacg 
      gccatcatt tacgccgtc ggaatgggc attttgatg 
      tttattggt tgtccaatg gatgatttg gcaggaatt 
      caaacccgt cacgagttt gcgactttc gacctcaac 
      tgcaacaaa tcgacgtgc gaatgcaac caggacaaa 
      ttctcgccg atctgtggc caggacggt aaaacctac 
      ctgtcgccc tgtcacgca ggctgcagg aactacacg 
      gaaaaagag ggaaaaatt gtggaatat tcggactgt 
      ctctgcgtg gcgcatgat gacaacgtt acgatttct 
      ttgcctgat agtgtttta tacgggaac gcgacgatc 
      ggctactgt gatgtgcaa tgtccgaat ttcacgtgg 
      tacataatc ctgttctcg atttttgtt ttcatccac 
      tcgacctcg gaagttggc tcgatgttg ctgattttg 
      cggtgtgtg gacccgagg gacaaggcc atggcgctg 
      gggctgatc cagttcgcc atcggccta ttcgggaac 
      gttccttgc cccattgtc tacggggct gttgtggac 
      tccgcttgc ctcgcaggg agtataaat ttcgtcgac 
      gagcaagta cccttggag gaagagctg acctcgatg 
      acgaaaaaa gcgttgacc gaacctgtg tctctacct 
      caatag
    mRNA sequence
    • >TC034513 RA
      tctctctgg tggtgcagt ttgaagaat tatcgaacg 
      gtgccttaa gtcttgcgt gtagtggct taatcgcgt 
      tgatcccgg agcgaggct ataagatgg ggtgcgtgg 
      tgcgggtca cggtggtcg tgttatcat cagtgttcc 
      gggcgtgcg tgtaactca tgcgtgaat taataaaaa 
      aagaaatga gtgcgcgtg ctttcggcc ggttatagt 
      gtcgcaaac tccggcgat gcgaagtcg aagtcgcat 
      tatataacg atttggggt aaaatcggc gtcctagcg 
      ccccagact ttgaagaat ttctcacat attgtttga 
      ttccgcgtc gcgttcgct ctctgactc tactctctc 
      tcccttcgc tttcgtttc gatggaatc cgaccgctt 
      ctgactggg actgggctc atgtctgaa ttgaatgaa 
      cgggaatct agcagtctg tcgtctgtc tgggtagag 
      tttcgactg tttcggcag tggtgatgt gacgtgctc 
      gcaatatat cacaatcgt tcgtctgac atgtgctat 
      taaatcagt gatcgagga ctattttct gccatacgg 
      tcatacgga cctagatgt ctttgccta cgaacagac 
      ggcgataga cccggccct ggaaaatgt cgaacggca 
      acagccacc tggccaacg gctcgatgt gggccgaag 
      gcaagccga gcaacaaca acggcccct ccaacggca 
      gcacccgcc tgggcatgg aggacatcc ccgaggagc 
      agacggccc tcacgaacc aggccgagg tgtaccgat 
      cgcagtgct tcgccagtg agggtgatt tcccgagcg 
      acagcgaca cggaatgcg gtctggggc cgttcgcgc 
      cccactggc tccaaatgt tcgcctcga agcaagcct 
      tcctcatag ttttctgca tcgcctggg tcctccagg 
      gcatgtatc acacgtact tcgtcagtg cgataacca 
      caatcgaaa aactctttc aaattcagt cgaaaatca 
      ccgggatta tcatgagtg ccaccgaaa tcggccaga 
      tcggctcgt cgctgctcc tgacgtact acggggggc 
      aggggcacc gccccaagt ggatcgcgt ggggatggt 
      tctgttcgc gacagttaa ttagcgcca atgatctca 
      cggggatca ccaaggacc cgaacgttt gcaaactcc 
      ccagtgcaa tagacgcgc tggagccca gcgcccact 
      ggggcaaca ccaccctcc cggtcaccg cgagcgggg 
      tgggggtct caaatattt tgcaaactt gtcatgagg 
      agctcgaca cggagcacc ggatccaac cctcggtca 
      cgaaaattg ttctcgcga tttttttta ttagcttgt 
      tgggggtgg gaatggggc aaacggctg tgtacactt 
      tggggattc cctacatcg acgacaacg tcgccagca 
      aggagtcgc cgctttatt tcgcgataa caatcggcg 
      tgcgaattc tgggtccgg ctctgggct tcatcgtcg 
      gatccctct gtactagcg tgtacgcgg atttatcag 
      tcgatccta aaatagata cgacagatc caaggtggg 
      ttggggcat ggtacttgg gtttggttt taatatcag 
      ggctactaa tgctggcct ctttcgcca tgttcgcct 
      tcccgaaac ggctgaaaa cggcgaaac cgacgccca 
      ggatacacc aacctggac gaaaacatc ctagcataa 
      gagacttcc ccaaaacgg tcaagcggc tgctcaaaa 
      acgatatcc taatgtttc ggaccgcaa gcagcgtct 
      tgcacattc ttccaatcg ctggattgt acacatttt 
      tgccaaagt atttagaat ctcagttca ggctaccca 
      ctccttcgg ccaatatga tatcaggag ttgggggca 
      ttttggtga tgggcgtcg gcataatta taagcgggg 
      tcttcatcc ttcgagtca aacctaacg ccaggtttg 
      tcgcagcct ggatcgcat tcacggcca tcatttacg 
      ccgtcggaa tgggcattt tgatgttta ttggttgtc 
      caatggatg atttggcag gaattcaaa cccgtcacg 
      agtttgcga ctttcgacc tcaactgca acaaatcga 
      cgtgcgaat gcaaccagg acaaattct cgccgatct 
      gtggccagg acggtaaaa cctacctgt cgccctgtc 
      acgcaggct gcaggaact acacggaaa aagagggaa 
      aaattgtgg aatattcgg actgtctct gcgtggcgc 
      atgatgaca acgttacga tttctttgc ctgatagtg 
      ttttatacg ggaacgcga cgatcggct actgtgatg 
      tgcaatgtc cgaatttca cgtggtaca taatcctgt 
      tctcgattt ttgttttca tccactcga cctcggaag 
      ttggctcga tgttgctga ttttgcggt gtgtggacc 
      cgagggaca aggccatgg cgctggggc tgatccagt 
      tcgccatcg gcctattcg ggaacgttc cttgcccca 
      ttgtctacg gggctgttg tggactccg cttgcctcg 
      cagggagta taaatttcg tcgacgagc aagtaccct 
      tggaggaag agctgacct cgatgacga aaaaagcgt 
      tgaccgaac ctgtgtctc tacctcaat agcacatat 
      tgaaggtgg aggatccac cgcccaaat tctgatcgc 
      gcgatcaga aacgactga aattttcta acggcagct 
      ttcacattt ttataacgt tttgtatag aaaagtgta 
      aatatggaa gattttatt ttagcaatt ttataggga 
      attttatac aaattttac aataaatag ttgacttcg 
      catgcttga aaattacat tgtgtttga gatctgaat 
      accatgcat tcataaact gtgatactt aaatatctg 
      cttgtcttt tccatcttg gttttgtaa atattcttt 
      taatttaaa ctattctca atactcctt gaccttgtg 
      aagttacat gcttttatc agtttagga caattgaaa 
      atactaatt gtatatttt tttatatta gtttgtagt 
      taccatatt tttaatttt gtatagttt tacatcgcc 
      ctacatcta tcaattgat taaagtgtg ccatacatt 
      caataattt gggatcctt tctgttgga ctttctcta 
      tttataacg tgttatatt gaagttttt gtgtttgtt 
      cacttgtac attacgaga taatttaaa tgattaaca 
      aacttgtta gaaaaagtt aaatactct tcctgttca 
      agctttact cattgttgt tattaaatt tttctaact 
      cttacacga aatgttgtt tccttttct catttacat 
      ctaagaaac aaatcaaat tacgggtat agcgtaact 
      atttattac tgttcgttt aagtggagt attttcaac 
      atcacatgt gcattataa ttattatta atatttaat 
      atttagtta agggcttac gcaaacaca aaacttcaa 
      caattggca gaaccttac aacaaagta gtttctatt 
      tgtatattt agtactgcc atatcttac ttatattgt 
      tttcttcag ggttacttt tttgttgat acttaactg 
      ttttaccta ttgttatat aacaattca agctcaagc 
      attttaatc tgtgattag ctcacattc atgcaccac 
      aactgttaa attgtgcaa ttttttaca gaagaaata 
      tcactctca tgttcacct aagtttaaa gaaaactgt 
      agtatatgt tgtacatta tgttaaaca ttaattaaa 
      gaagcatca ccttgcgtg aatacttga taatgtaac 
      gattttaca taattgtac ctatgcaaa atatattca 
      ttaaataag c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY