Status
Please wait ...
Gene information
General information
TC004678 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 170411..171469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004678
    iBeetle screen iB_03812

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004678 RA
      MATNLKRKL SEKKPDVLE KVAKGGPNR EEAISRLIN 
      AVSEGPYGK FVITLPATG TSPYVMLQN AATYMNLPL 
      NVVKSESEI NGFELLING EEFAKGQFD NIQEARKGI 
      ADQALNKLK ETCFFITRK SEYVNVTKD NLHEKEAPS 
      VAAPTAFAD STAYKIMAK MGWSGGGLG AQEQGNPDS 
      ITVYENVNR QGLGTTNLT KQITEKLGN FAKSSSLLT 
      LVFDSDFTK EERVFIHKA ARKFNLKTK SIGRESRKI 
      TVSKKITRM DIVRELLRS GGEDGFYKL HVPTNFAHL 
      FTV
    CDS sequence
    • >TC004678 RA
      atggcaaca aatttgaaa cgcaaatta agcgaaaaa 
      aaaccggac gtgcttgaa aaagtggcc aaagggggg 
      cccaaccgt gaagaagca atctcaagg cttataaat 
      gcagtgtca gaagggccg tacggtaaa tttgttata 
      accctacca gcaactggc acaagccct tatgttatg 
      ctccaaaac gcggctact tacatgaac ttgccccta 
      aacgtcgtc aaatctgaa tctgagata aacggtttt 
      gaattgctc ataaacggc gaggagttt gcgaaagga 
      caatttgat aatatacaa gaggcgcgc aaagggata 
      gctgatcaa gcgttgaat aagctaaaa gagacgtgc 
      tttttcata acaaggaaa agtgagtat gttaacgta 
      actaaggat aatttgcac gaaaaggag gccccgagt 
      gtggcagcc cccactgcc tttgccgac agcacggcg 
      tacaagata atggccaaa atggggtgg tcggggggc 
      ggactgggg gcccaggaa cagggcaat cctgactcg 
      atcacagtt tacgaaaat gtcaacaga cagggcttg 
      gggaccacg aatttaacg aaacaaatc acagagaaa 
      ttggggaat tttgccaaa tcgtccagt ttgttaacg 
      ctagttttt gactcagat tttacaaag gaggagcgc 
      gttttcatc cacaaggcc gcgaggaaa tttaatctc 
      aaaactaag agtataggg cgcgagagt cggaaaata 
      acagtttcg aaaaaaatt accaggatg gatattgtg 
      agagagttg ctgaggtca ggcggcgag gacgggttt 
      tacaagttg cacgtcccg accaatttt gcgcattta 
      tttactgtg taa
    mRNA sequence
    • >TC004678 RA
      cattttttt gaaatggca acaaatttg aaacgcaaa 
      ttaagcgaa aaaaaaccg gacgtgctt gaaaaagtg 
      gccaaaggg gggcccaac cgtgaagaa gcaatctca 
      aggcttata aatgcagtg tcagaaggg ccgtacggt 
      aaatttgtt ataacccta ccagcaact ggcacaagc 
      ccttatgtt atgctccaa aacgcggct acttacatg 
      aacttgccc ctaaacgtc gtcaaatct gaatctgag 
      ataaacggt tttgaattg ctcataaac ggcgaggag 
      tttgcgaaa ggacaattt gataatata caagaggcg 
      cgcaaaggg atagctgat caagcgttg aataagcta 
      aaagagacg tgctttttc ataacaagg aaaagtgag 
      tatgttaac gtaactaag gataatttg cacgaaaag 
      gaggccccg agtgtggca gcccccact gcctttgcc 
      gacagcacg gcgtacaag ataatggcc aaaatgggg 
      tggtcgggg ggcggactg ggggcccag gaacagggc 
      aatcctgac tcgatcaca gtttacgaa aatgtcaac 
      agacagggc ttggggacc acgaattta acgaaacaa 
      atcacagag aaattgggg aattttgcc aaatcgtcc 
      agtttgtta acgctagtt tttgactca gattttaca 
      aaggaggag cgcgttttc atccacaag gccgcgagg 
      aaatttaat ctcaaaact aagagtata gggcgcgag 
      agtcggaaa ataacagtt tcgaaaaaa attaccagg 
      atggatatt gtgagagag ttgctgagg tcaggcggc 
      gaggacggg ttttacaag ttgcacgtc ccgaccaat 
      tttgcgcat ttatttact gtgtaaata ttcaaaaat 
      aataaattt aatttgagt tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY