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Gene information
General information
TC004674 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1: 142321..145265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004674
    iBeetle screen iB_03811
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004674 RA
      MYLIIFISA SVILYQYQL SNAWLISQN SQEFHVLSP 
      KTGDKPRSG SLFNASSIS NTTPCFQEP EDLLPPLYV 
      ITPTYRRPE QLAELTRLS HTLMLVKNV FWIVVEDAT 
      NKNHLVAEL LKKTGLKHE HLLAPMPEL YRKKKGPKP 
      RGVSNRNRG LQWIRQNAK TGVFYFADD DNTYDLQLF 
      TEIRYTKRV SMFPVGLIT KLGVSSPIV RNGRFSGFY 
      DGWVAGRKF PVDMAGFAV AVEFLLQRP KALMPFRPG 
      FEEDGFLKS LEPFEPQDI ELLASNCTK ILVWHTQTK 
      KNEPSQPLD LAKYNSTNL VMLKQILV
    CDS sequence
    • >TC004674 RA
      atgtatctc ataattttc ataagcgca tcggtgatt 
      ctgtaccaa taccagttg tcgaacgcg tggctgatc 
      tcccagaat tcgcaagag ttccacgtt ctgagcccc 
      aaaaccggc gacaaaccc cgcagtggc tccctgttt 
      aacgcctct tccattagc aatacgaca ccctgtttt 
      caagagccg gaagactta ttgccgccc ctgtatgta 
      ataaccccg acctatcgc aggccggaa caactcgcc 
      gaattgacc cgactgtcc cacactctg atgctcgtc 
      aaaaacgta ttttggata gtcgtcgaa gacgcaacg 
      aacaaaaac cacctagtt gccgagctt ttgaaaaaa 
      acgggcttg aaacacgag cacctactc gcgccgatg 
      cctgaactt taccgcaag aaaaaaggg cccaaacct 
      cgcggcgtc tcgaaccgg aaccgaggc ttgcagtgg 
      atacgccaa aacgccaaa accggagtt ttttatttc 
      gccgatgac gacaacact tacgacctc cagttattt 
      acagagatt cgctacaca aaacgcgtg tcgatgttt 
      cccgttggt ctgatcacc aaactgggc gtgagctcg 
      cccatcgtc cgcaacggt cgtttttcg gggttttat 
      gacggatgg gtggccggt cgcaagttt cccgttgat 
      atggccggc tttgccgtc gcggttgag tttctgttg 
      cagcgtccg aaagcttta atgccgttc aggcctggt 
      ttcgaagag gacggcttt ttgaaaagt ctcgaacct 
      tttgaacct caagacatc gaattgtta gcttcaaat 
      tgcacaaaa attttagta tggcacacg caaacgaag 
      aagaatgaa ccgtcacaa cccttagat ctcgccaag 
      tataatagc actaactta gtcatgctc aaacaaata 
      ctggtatag
    mRNA sequence
    • >TC004674 RA
      ggtgcaaaa gacacaaaa taatgtttt gtagcattg 
      tgcatcaat agtagaggc agcaagttg tgtatttcg 
      atgaaattt gaataaaac tgtgcagga tcgagtgtc 
      atttcgatg atgtagcgc tgggtacaa tacgctttg 
      aaaatgttc ctgtaatta ttgtcgaga tagctccaa 
      aatatgagg ctttccatc aaaatgtat ctcataatt 
      ttcataagc gcatcggtg attctgtac caataccag 
      ttgtcgaac gcgtggctg atctcccag aattcgcaa 
      gagttccac gttctgagc cccaaaacc ggcgacaaa 
      ccccgcagt ggctccctg tttaacgcc tcttccatt 
      agcaatacg acaccctgt tttcaagag ccggaagac 
      ttattgccg cccctgtat gtaataacc ccgacctat 
      cgcaggccg gaacaactc gccgaattg acccgactg 
      tcccacact ctgatgctc gtcaaaaac gtattttgg 
      atagtcgtc gaagacgca acgaacaaa aaccaccta 
      gttgccgag cttttgaaa aaaacgggc ttgaaacac 
      gagcaccta ctcgcgccg atgcctgaa ctttaccgc 
      aagaaaaaa gggcccaaa cctcgcggc gtctcgaac 
      cggaaccga ggcttgcag tggatacgc caaaacgcc 
      aaaaccgga gttttttat ttcgccgat gacgacaac 
      acttacgac ctccagtta tttacagag attcgctac 
      acaaaacgc gtgtcgatg tttcccgtt ggtctgatc 
      accaaactg ggcgtgagc tcgcccatc gtccgcaac 
      ggtcgtttt tcggggttt tatgacgga tgggtggcc 
      ggtcgcaag tttcccgtt gatatggcc ggctttgcc 
      gtcgcggtt gagtttctg ttgcagcgt ccgaaagct 
      ttaatgccg ttcaggcct ggtttcgaa gaggacggc 
      tttttgaaa agtctcgaa ccttttgaa cctcaagac 
      atcgaattg ttagcttca aattgcaca aaaatttta 
      gtatggcac acgcaaacg aagaagaat gaaccgtca 
      caaccctta gatctcgcc aagtataat agcactaac 
      ttagtcatg ctcaaacaa atactggta taggcaatc 
      agtccggaa gtccattta ctgtacaaa tttataagg 
      gttctgtag ttaacgact tgcatatcg gttcagttc 
      gagtttatt tatgtgata cttttgtat gttgtttcg 
      ttttatatt gacgtggat attttttat gaaagaggc 
      tgtggatga cttttttac catcattcg ctatttaga 
      acatgtaag ataaacaac aatcatgta gcgttttgc 
      gaaa