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Gene information
General information
TC004673 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1: 123101..135483
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004673
    iBeetle screen iB_03810
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment posterior everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004673 RA
      MAAESSTSL PEQSEPPSR SLNFSIAKI MEPDNKSKD 
      DSRSKIVNI PYPTPLESA FKKYVPNML RSQDLLQQY 
      PLVYYHPSQ LMLSSVYSS ESSTPPNPP PSLSTPIQF 
      PPKPCPPKR SESKPSIVT PSSTAKQKS FECGECGKV 
      FNAHYNLTR HMPVHTGAR PFVCKICGK GFRQASTLC 
      RHKIIHTSE KPHKCHTCG KAFNRSSTL NTHARIHAG 
      YKPFVCEYC GKGFHQKGN YKNHKLTHS GEKAYKCTV 
      CSKAFHQIY NLTFHMHTH NDKKPFTCR VCGKGFCRN 
      FDLKKHMRK LHESTSASD SDFVANAAP PAVYPNERD 
      LHHFISPFL RPPVPVYSK LL
    CDS sequence
    • >TC004673 RA
      atggcggcc gagagttca acgagtctc cccgagcag 
      tcggagccc ccctctcga agcctcaac ttttcgata 
      gcaaaaata atggagccc gacaacaaa tcaaaagac 
      gacagtcgt agcaaaatt gtaaatatc ccgtatcca 
      actccccta gaatcagcg ttcaagaaa tacgttcca 
      aacatgttg cgatcgcag gacttgtta caacaatac 
      cccctagtg tactaccac cctagtcaa ttaatgcta 
      agttcagtg tactcatcc gagagcagt acccctccc 
      aaccctcct cccagcctc agtaccccc attcagttc 
      ccccccaag ccgtgcccc cccaagcgc agcgagtcc 
      aagccctcc atcgtgacc ccctccagc accgccaag 
      cagaagagc ttcgagtgt ggcgagtgt ggtaaagtt 
      ttcaacgcg cattacaac ctcacccgc catatgccg 
      gtccacacc ggggcacgg cctttcgtc tgcaaaatc 
      tgcgggaag ggctttcgg caagcttcc actctgtgt 
      cgccacaaa atcatccac acgtccgag aagccccac 
      aaatgccac acttgcggg aaggccttc aaccgctcc 
      tccaccctc aacacgcac gcgcggatc cacgcgggc 
      tacaagccc ttcgtgtgc gagtactgc gggaagggc 
      ttccaccag aagggcaac tacaagaac cacaagctc 
      acccacagc ggggagaag gcctacaag tgtacagtg 
      tgctccaag gccttccac cagatctac aacttgacc 
      ttccacatg cacacgcac aacgacaag aagcccttc 
      acttgtcgc gtttgcggc aaagggttt tgtcggaat 
      tttgacttg aagaagcac atgcgcaag ctgcatgag 
      agtacttcc gcctccgat tcggacttc gtggccaat 
      gccgcgccg ccggctgtt tatccgaac gaaagggac 
      ttgcaccat ttcatcagt ccgttttta cggccgcct 
      gtgccggtc tattcgaaa ttattgtga
    mRNA sequence
    • >TC004673 RA
      gtggcctcc taattatgc aaatggccc tgctcacct 
      aatgttgcc accatcagt ctcttgttt gcgcttttc 
      ccaaaacta ccaggccaa agttcaacc agacatcag 
      ttttcagcc tcagattgc cttttgatg ctacaagcc 
      acaagtgac gaatcaaac aaaacctag tgtgatcgg 
      ttcttagct actgtaaaa tgcttttcg ttgatgtga 
      acttgatga ctctcaaag actgtttta gtgattttt 
      acgattaat tacctctca actttatgc tatattgat 
      tgagtgaag atggcggcc gagagttca acgagtctc 
      cccgagcag tcggagccc ccctctcga agcctcaac 
      ttttcgata gcaaaaata atggagccc gacaacaaa 
      tcaaaagac gacagtcgt agcaaaatt gtaaatatc 
      ccgtatcca actccccta gaatcagcg ttcaagaaa 
      tacgttcca aacatgttg cgatcgcag gacttgtta 
      caacaatac cccctagtg tactaccac cctagtcaa 
      ttaatgcta agttcagtg tactcatcc gagagcagt 
      acccctccc aaccctcct cccagcctc agtaccccc 
      attcagttc ccccccaag ccgtgcccc cccaagcgc 
      agcgagtcc aagccctcc atcgtgacc ccctccagc 
      accgccaag cagaagagc ttcgagtgt ggcgagtgt 
      ggtaaagtt ttcaacgcg cattacaac ctcacccgc 
      catatgccg gtccacacc ggggcacgg cctttcgtc 
      tgcaaaatc tgcgggaag ggctttcgg caagcttcc 
      actctgtgt cgccacaaa atcatccac acgtccgag 
      aagccccac aaatgccac acttgcggg aaggccttc 
      aaccgctcc tccaccctc aacacgcac gcgcggatc 
      cacgcgggc tacaagccc ttcgtgtgc gagtactgc 
      gggaagggc ttccaccag aagggcaac tacaagaac 
      cacaagctc acccacagc ggggagaag gcctacaag 
      tgtacagtg tgctccaag gccttccac cagatctac 
      aacttgacc ttccacatg cacacgcac aacgacaag 
      aagcccttc acttgtcgc gtttgcggc aaagggttt 
      tgtcggaat tttgacttg aagaagcac atgcgcaag 
      ctgcatgag agtacttcc gcctccgat tcggacttc 
      gtggccaat gccgcgccg ccggctgtt tatccgaac 
      gaaagggac ttgcaccat ttcatcagt ccgttttta 
      cggccgcct gtgccggtc tattcgaaa ttattgtga 
      ccgttttaa tttcactac ttgatgatc tcttatcga 
      tgtgcaata ttgcagggc tggccgcat ggggcacca 
      ctgcttaga ctaaattgt aaaaatgtg taatttagt 
      accacatgt aatactcaa ataaatgtt atttaatac 
      t