Status
Please wait ...
Gene information
General information
TC004667 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 69771..74466
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004667
    iBeetle screen iB_03809

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004667 RA
      MDLSPIINE FDTPFLVDS GTESFTESP VAEEESEHS 
      EDSTVCDID SSCIQKCHI LDKEVSVDE DILAVPLEP 
      IRTQTEPSI VEPNEQDET ALPSILRRE SSTSSTVRK 
      VSFPKEETE LATYREPEN DCPWTINTY LPPEKILQI 
      YQASCKQHQ TIELDIIKN LIMEIKNNT NHSAVLNLS 
      MINITNEVN ETLEDLLKV SNFRTLVLY ECKFTSETI 
      SEFFNMLEY YGSVCDIEV AMNFDETAW KCFCNACTN 
      LTVLEAISF KGMVINENY MRMLLNAVK SNTRITTLK 
      FDACMLIKL PSFYLVESL MTNTTLCEL YLPSTGLYT 
      KEADCLGRF LQKNFTLKV LDISNNFIG DRGLEVLAK 
      GLTKQTTAG CGLSVLVVF NNQITEKSG PVICSIISE 
      CKNLHTLNI GYNHLTDAV ISDIAPSLE VTTSLEGLG 
      LQCTLLTCK GIDYLANAI ENNNSLQKI NLKGNKAIQ 
      VPGLERLCQ ALTSSKISK IEIDETNRS SHDPQAYVQ 
      LVKKMNAIC TVNKNFKES SCDEIATIS QLVSRKVSL 
      SCEPRYVVP EPAPGATPL SVDTPPILS PVPAARPPK 
      NRFQITKVQ DPNTRPISR FKVTRVIPS PDEDLAIGK 
      FANVRSSVS SNDSVDSLQ LDVDSDRD
    • >TC004667 RB
      MVRKRDSGT ESFTESPVA EEESEHSED STVCDIDSS 
      CIQKCHILD KEVSVDEDI LAVPLEPIR TQTEPSIVE 
      PNEQDETAL PSILRRESS TSSTVRKVS FPKEETELA 
      TYREPENDC PWTINTYLP PEKILQIYQ ASCKQHQTI 
      ELDIIKNLI MEIKNNTNH SAVLNLSMI NITNEVNET 
      LEDLLKVSN FRTLVLYEC KFTSETISE FFNMLEYYG 
      SVCDIEVAM NFDETAWKC FCNACTNLT VLEAISFKG 
      MVINENYMR MLLNAVKSN TRITTLKFD ACMLIKLPS 
      FYLVESLMT NTTLCELYL PSTGLYTKE ADCLGRFLQ 
      KNFTLKVLD ISNNFIGDR GLEVLAKGL TKQTTAGCG 
      LSVLVVFNN QITEKSGPV ICSIISECK NLHTLNIGY 
      NHLTDAVIS DIAPSLEVT TSLEGLGLQ CTLLTCKGI 
      DYLANAIEN NNSLQKINL KGNKAIQVP GLERLCQAL 
      TSSKISKIE IDETNRSSH DPQAYVQLV KKMNAICTV 
      NKNFKESSC DEIATISQL VSRKVSLSC EPRYVVPEP 
      APGATPLSV DTPPILSPV PAARPPKNR FQITKVQDP 
      NTRPISRFK VTRVIPSPD EDLAIGKFA NVRSSVSSN 
      DSVDSLQLD VDSDRD
    CDS sequence
    • >TC004667 RA
      atggatttg tctccaatc ataaatgaa tttgacaca 
      cctttcctt gtagacagt ggcactgaa agttttacc 
      gaaagccct gttgcagaa gaagagagc gagcactcg 
      gaggactcc acagtctgc gacattgat tcgtcgtgc 
      atccaaaaa tgccacatt ttggacaaa gaggtctca 
      gtggacgag gacattctg gctgttcct ctggagccc 
      attcgcacc caaaccgag ccaagtatt gtggaaccg 
      aacgagcag gatgagacc gccctcccg tccatttta 
      cggagggag tcctccacc tcgagcacc gtcaggaag 
      gtctccttc cccaaggag gagacggag ttggccacc 
      tacagggag cccgaaaac gactgtcct tggaccata 
      aacacgtat ttgcccccc gaaaaaatc ctacaaatc 
      taccaagcc tcctgcaag cagcatcaa accatagag 
      ctggacata atcaaaaat ctgatcatg gaaataaaa 
      aacaacaca aaccattca gcagtattg aacttatcg 
      atgataaac ataacgaat gaggtgaac gagacgttg 
      gaagacttg ttaaaagtg tcgaacttc cgcaccttg 
      gtgttatac gaatgtaag ttcacgtcg gagacgata 
      agcgaattc ttcaacatg ttggaatat tacggttcg 
      gtgtgtgat attgaggtc gcgatgaat tttgacgaa 
      acggcctgg aagtgcttc tgcaacgct tgtacaaat 
      ctcaccgtc ttggaggcc atctccttc aaagggatg 
      gtaatcaac gagaactac atgcggatg ttacttaac 
      gccgttaag agcaacact agaatcacc acattgaag 
      tttgacgct tgtatgctt ataaagtta ccgtcgttt 
      tacttagtc gaaagtcta atgacaaac actactctc 
      tgcgaactg tacctgccc tcgactgga ctgtacaca 
      aaagaagct gactgtttg ggccgtttc ctgcagaaa 
      aacttcaca cttaaagtc ctagacata agtaataat 
      tttattggg gacaggggg ctggaagtc ctagctaaa 
      ggactcacc aaacagacg acggcaggc tgtggcttg 
      tcggtttta gtcgttttc aacaaccaa atcacggag 
      aaaagtgga ccggttatc tgtagtata atttccgag 
      tgtaaaaat ttgcacacc cttaacatc ggttataac 
      cacctgacg gatgcggtt atttccgac attgccccc 
      agtttggag gtgacgacg tctctggag ggcctagga 
      ctccagtgc acccttctg acgtgcaaa ggaattgac 
      tacctcgcg aatgccatc gagaataac aactcgttg 
      caaaaaatt aacctgaag gggaacaaa gcgattcaa 
      gtccctggc ttagagcgt ctgtgccag gcgttaaca 
      tcgtcgaag atttcgaaa atcgagatt gacgaaacg 
      aaccgttcg tctcacgac ccccaagcc tacgtccaa 
      ctcgtgaaa aaaatgaac gcaatttgc acagtcaac 
      aagaatttc aaagagtca agctgcgac gaaatcgcc 
      acgatttcg cagctcgtt tcgcggaag gtttccctg 
      agttgcgag ccccgttac gtcgttccg gagcccgcg 
      ccgggggct acgcccctc tcggtcgac actccgccg 
      attctgtcg cccgttccg gcggcacgg ccccccaag 
      aaccggttc caaataacc aaagtacaa gaccccaac 
      acgcgcccg atctcgcgg tttaaagtt acgagagtt 
      attccgtcg ccggatgaa gacttggcc attggcaag 
      ttcgcaaat gtgaggagc agtgtgtcg tcgaatgac 
      agcgtcgac tccctccaa cttgacgtg gatagtgat 
      agagattag
    • >TC004667 RB
      atggttcgc aaacgagac agtggcact gaaagtttt 
      accgaaagc cctgttgca gaagaagag agcgagcac 
      tcggaggac tccacagtc tgcgacatt gattcgtcg 
      tgcatccaa aaatgccac attttggac aaagaggtc 
      tcagtggac gaggacatt ctggctgtt cctctggag 
      cccattcgc acccaaacc gagccaagt attgtggaa 
      ccgaacgag caggatgag accgccctc ccgtccatt 
      ttacggagg gagtcctcc acctcgagc accgtcagg 
      aaggtctcc ttccccaag gaggagacg gagttggcc 
      acctacagg gagcccgaa aacgactgt ccttggacc 
      ataaacacg tatttgccc cccgaaaaa atcctacaa 
      atctaccaa gcctcctgc aagcagcat caaaccata 
      gagctggac ataatcaaa aatctgatc atggaaata 
      aaaaacaac acaaaccat tcagcagta ttgaactta 
      tcgatgata aacataacg aatgaggtg aacgagacg 
      ttggaagac ttgttaaaa gtgtcgaac ttccgcacc 
      ttggtgtta tacgaatgt aagttcacg tcggagacg 
      ataagcgaa ttcttcaac atgttggaa tattacggt 
      tcggtgtgt gatattgag gtcgcgatg aattttgac 
      gaaacggcc tggaagtgc ttctgcaac gcttgtaca 
      aatctcacc gtcttggag gccatctcc ttcaaaggg 
      atggtaatc aacgagaac tacatgcgg atgttactt 
      aacgccgtt aagagcaac actagaatc accacattg 
      aagtttgac gcttgtatg cttataaag ttaccgtcg 
      ttttactta gtcgaaagt ctaatgaca aacactact 
      ctctgcgaa ctgtacctg ccctcgact ggactgtac 
      acaaaagaa gctgactgt ttgggccgt ttcctgcag 
      aaaaacttc acacttaaa gtcctagac ataagtaat 
      aattttatt ggggacagg gggctggaa gtcctagct 
      aaaggactc accaaacag acgacggca ggctgtggc 
      ttgtcggtt ttagtcgtt ttcaacaac caaatcacg 
      gagaaaagt ggaccggtt atctgtagt ataatttcc 
      gagtgtaaa aatttgcac acccttaac atcggttat 
      aaccacctg acggatgcg gttatttcc gacattgcc 
      cccagtttg gaggtgacg acgtctctg gagggccta 
      ggactccag tgcaccctt ctgacgtgc aaaggaatt 
      gactacctc gcgaatgcc atcgagaat aacaactcg 
      ttgcaaaaa attaacctg aaggggaac aaagcgatt 
      caagtccct ggcttagag cgtctgtgc caggcgtta 
      acatcgtcg aagatttcg aaaatcgag attgacgaa 
      acgaaccgt tcgtctcac gacccccaa gcctacgtc 
      caactcgtg aaaaaaatg aacgcaatt tgcacagtc 
      aacaagaat ttcaaagag tcaagctgc gacgaaatc 
      gccacgatt tcgcagctc gtttcgcgg aaggtttcc 
      ctgagttgc gagccccgt tacgtcgtt ccggagccc 
      gcgccgggg gctacgccc ctctcggtc gacactccg 
      ccgattctg tcgcccgtt ccggcggca cggcccccc 
      aagaaccgg ttccaaata accaaagta caagacccc 
      aacacgcgc ccgatctcg cggtttaaa gttacgaga 
      gttattccg tcgccggat gaagacttg gccattggc 
      aagttcgca aatgtgagg agcagtgtg tcgtcgaat 
      gacagcgtc gactccctc caacttgac gtggatagt 
      gatagagat tag
    mRNA sequence
    • >TC004667 RA
      gaattgttc aatacaaaa aaaccaaaa caagccgcc 
      ggggccatg ccggggcct gctatcaat acaaaacaa 
      aaaatacgt ggttgtgcg ttcgttttt gggataatt 
      gaatgaatt ggtcatggg gcatttttt gtcggttgg 
      ggcgcttag tcgcttcgt gtgtgtaaa aaagcgcat 
      atttgcagc cgcggttca cacgcttat tgcgataaa 
      agtgcgtta tatctgtgt ttgtcttgg aggagtgta 
      gcctagtgt tacgtcgca atctaggtt tcatcttat 
      gtcagtggt tgttgcgct atcatccat gttgctttt 
      tgacttatt tttactttt ggattgtga atttgtgtg 
      tgtgttaaa aacccgact aaaaaatgc ttttccgag 
      aatgaggtt ggtttaatg aacagttcc gcaccggac 
      tttgccaat attgtggat gtggtgact taccaattc 
      aagaatggt tcgcaaacg agatcacaa taaaaagaa 
      aggtccgtt ctaaatgat gaacgtaat ggatttgtc 
      tccaatcat aaatgaatt tgacacacc tttccttgt 
      agacagtgg cactgaaag ttttaccga aagccctgt 
      tgcagaaga agagagcga gcactcgga ggactccac 
      agtctgcga cattgattc gtcgtgcat ccaaaaatg 
      ccacatttt ggacaaaga ggtctcagt ggacgagga 
      cattctggc tgttcctct ggagcccat tcgcaccca 
      aaccgagcc aagtattgt ggaaccgaa cgagcagga 
      tgagaccgc cctcccgtc cattttacg gagggagtc 
      ctccacctc gagcaccgt caggaaggt ctccttccc 
      caaggagga gacggagtt ggccaccta cagggagcc 
      cgaaaacga ctgtccttg gaccataaa cacgtattt 
      gccccccga aaaaatcct acaaatcta ccaagcctc 
      ctgcaagca gcatcaaac catagagct ggacataat 
      caaaaatct gatcatgga aataaaaaa caacacaaa 
      ccattcagc agtattgaa cttatcgat gataaacat 
      aacgaatga ggtgaacga gacgttgga agacttgtt 
      aaaagtgtc gaacttccg caccttggt gttatacga 
      atgtaagtt cacgtcgga gacgataag cgaattctt 
      caacatgtt ggaatatta cggttcggt gtgtgatat 
      tgaggtcgc gatgaattt tgacgaaac ggcctggaa 
      gtgcttctg caacgcttg tacaaatct caccgtctt 
      ggaggccat ctccttcaa agggatggt aatcaacga 
      gaactacat gcggatgtt acttaacgc cgttaagag 
      caacactag aatcaccac attgaagtt tgacgcttg 
      tatgcttat aaagttacc gtcgtttta cttagtcga 
      aagtctaat gacaaacac tactctctg cgaactgta 
      cctgccctc gactggact gtacacaaa agaagctga 
      ctgtttggg ccgtttcct gcagaaaaa cttcacact 
      taaagtcct agacataag taataattt tattgggga 
      cagggggct ggaagtcct agctaaagg actcaccaa 
      acagacgac ggcaggctg tggcttgtc ggttttagt 
      cgttttcaa caaccaaat cacggagaa aagtggacc 
      ggttatctg tagtataat ttccgagtg taaaaattt 
      gcacaccct taacatcgg ttataacca cctgacgga 
      tgcggttat ttccgacat tgcccccag tttggaggt 
      gacgacgtc tctggaggg cctaggact ccagtgcac 
      ccttctgac gtgcaaagg aattgacta cctcgcgaa 
      tgccatcga gaataacaa ctcgttgca aaaaattaa 
      cctgaaggg gaacaaagc gattcaagt ccctggctt 
      agagcgtct gtgccaggc gttaacatc gtcgaagat 
      ttcgaaaat cgagattga cgaaacgaa ccgttcgtc 
      tcacgaccc ccaagccta cgtccaact cgtgaaaaa 
      aatgaacgc aatttgcac agtcaacaa gaatttcaa 
      agagtcaag ctgcgacga aatcgccac gatttcgca 
      gctcgtttc gcggaaggt ttccctgag ttgcgagcc 
      ccgttacgt cgttccgga gcccgcgcc gggggctac 
      gcccctctc ggtcgacac tccgccgat tctgtcgcc 
      cgttccggc ggcacggcc ccccaagaa ccggttcca 
      aataaccaa agtacaaga ccccaacac gcgcccgat 
      ctcgcggtt taaagttac gagagttat tccgtcgcc 
      ggatgaaga cttggccat tggcaagtt cgcaaatgt 
      gaggagcag tgtgtcgtc gaatgacag cgtcgactc 
      cctccaact tgacgtgga tagtgatag agattagta 
      attaggcag attttgtat tagttctgt gataagaat 
      tttatacat aagttagtg gttcaaatt ttattcatt 
      actgcctta acgcgaaat tatagttca aatgtattt 
      tcttgcagc agttgagtt tccttagcc gtgatattt 
      ttgcagggt ttgatggaa gggcccaag gttctccct 
      taacttaag ttagttacg cagctatga cttaagtac 
      gtatcgttt tggcgtgca taccttcct gtttgttca 
      tgcatgcac gattccagt caaccaaac atccctatt 
      ttgttcaaa actgttaag tgataaagg gttaagtgt 
      ttcatgtgc aattctcgt acctggcgg tacaaagaa 
      attatttat tgtaataac acggagtta gatttagca 
      accgtacaa acctcagtt ctttgttgt attgttttg 
      ggcatgctg gagcatgtt tttggttat acgtttttt 
      taattagct ttaaagaga gtaaggccg acagaaaca 
      atgtacata ccggtcgaa taatttgtt ttgagacga 
      cgtttctgg agtcttgcc gactactca atgtgttta 
      aatagggaa tagtaactt aaccagttg taaaataaa 
      tatattttt tatatgaac gttgttagt aacttggga 
      tacgataat tagaaatag caaattttg gtacaaagt 
      gtagaaagt caaagttta ttattgcaa tgttcgagc 
      aaattctgt tgtttaaag catgcacta tgcatttca 
      ttactatag tttatattt atgtgtatg tgataaatg 
      catgtgata aataaaata tcttaaaga gtattttaa 
      caaataacc atattgtag agaaacttg taattaatt 
      atttataat aaattgaac gtttattga accgtgtca 
      tacacagct aagtaccaa cttttgact acctatcat 
      cgaccagtt ttgtgccta aactcgcac tcacagggt 
      ctcaatgta ttgttactt accgttgga gtgttgtta 
      acatccctc tgtttgtca cttgtttgt attattaac 
      tcaataaat aagatcaga caat
    • >TC004667 RB
      gaattgttc aatacaaaa aaaccaaaa caagccgcc 
      ggggccatg ccggggcct gctatcaat acaaaacaa 
      aaaatacgt ggttgtgcg ttcgttttt gggataatt 
      gaatgaatt ggtcatggg gcatttttt gtcggttgg 
      ggcgcttag tcgcttcgt gtgtgtaaa aaagcgcat 
      atttgcagc cgcggttca cacgcttat tgcgataaa 
      agtgcgtta tatctgtgt ttgtcttgg aggagtgta 
      gcctagtgt tacgtcgca atctaggtt tcatcttat 
      gtcagtggt tgttgcgct atcatccat gttgctttt 
      tgacttatt tttactttt ggattgtga atttgtgtg 
      tgtgttaaa aacccgact aaaaaatgc ttttccgag 
      aatgaggtt ggtttaatg aacagttcc gcaccggac 
      tttgccaat attgtggat gtggtgact taccaattc 
      aagaatggt tcgcaaacg agacagtgg cactgaaag 
      ttttaccga aagccctgt tgcagaaga agagagcga 
      gcactcgga ggactccac agtctgcga cattgattc 
      gtcgtgcat ccaaaaatg ccacatttt ggacaaaga 
      ggtctcagt ggacgagga cattctggc tgttcctct 
      ggagcccat tcgcaccca aaccgagcc aagtattgt 
      ggaaccgaa cgagcagga tgagaccgc cctcccgtc 
      cattttacg gagggagtc ctccacctc gagcaccgt 
      caggaaggt ctccttccc caaggagga gacggagtt 
      ggccaccta cagggagcc cgaaaacga ctgtccttg 
      gaccataaa cacgtattt gccccccga aaaaatcct 
      acaaatcta ccaagcctc ctgcaagca gcatcaaac 
      catagagct ggacataat caaaaatct gatcatgga 
      aataaaaaa caacacaaa ccattcagc agtattgaa 
      cttatcgat gataaacat aacgaatga ggtgaacga 
      gacgttgga agacttgtt aaaagtgtc gaacttccg 
      caccttggt gttatacga atgtaagtt cacgtcgga 
      gacgataag cgaattctt caacatgtt ggaatatta 
      cggttcggt gtgtgatat tgaggtcgc gatgaattt 
      tgacgaaac ggcctggaa gtgcttctg caacgcttg 
      tacaaatct caccgtctt ggaggccat ctccttcaa 
      agggatggt aatcaacga gaactacat gcggatgtt 
      acttaacgc cgttaagag caacactag aatcaccac 
      attgaagtt tgacgcttg tatgcttat aaagttacc 
      gtcgtttta cttagtcga aagtctaat gacaaacac 
      tactctctg cgaactgta cctgccctc gactggact 
      gtacacaaa agaagctga ctgtttggg ccgtttcct 
      gcagaaaaa cttcacact taaagtcct agacataag 
      taataattt tattgggga cagggggct ggaagtcct 
      agctaaagg actcaccaa acagacgac ggcaggctg 
      tggcttgtc ggttttagt cgttttcaa caaccaaat 
      cacggagaa aagtggacc ggttatctg tagtataat 
      ttccgagtg taaaaattt gcacaccct taacatcgg 
      ttataacca cctgacgga tgcggttat ttccgacat 
      tgcccccag tttggaggt gacgacgtc tctggaggg 
      cctaggact ccagtgcac ccttctgac gtgcaaagg 
      aattgacta cctcgcgaa tgccatcga gaataacaa 
      ctcgttgca aaaaattaa cctgaaggg gaacaaagc 
      gattcaagt ccctggctt agagcgtct gtgccaggc 
      gttaacatc gtcgaagat ttcgaaaat cgagattga 
      cgaaacgaa ccgttcgtc tcacgaccc ccaagccta 
      cgtccaact cgtgaaaaa aatgaacgc aatttgcac 
      agtcaacaa gaatttcaa agagtcaag ctgcgacga 
      aatcgccac gatttcgca gctcgtttc gcggaaggt 
      ttccctgag ttgcgagcc ccgttacgt cgttccgga 
      gcccgcgcc gggggctac gcccctctc ggtcgacac 
      tccgccgat tctgtcgcc cgttccggc ggcacggcc 
      ccccaagaa ccggttcca aataaccaa agtacaaga 
      ccccaacac gcgcccgat ctcgcggtt taaagttac 
      gagagttat tccgtcgcc ggatgaaga cttggccat 
      tggcaagtt cgcaaatgt gaggagcag tgtgtcgtc 
      gaatgacag cgtcgactc cctccaact tgacgtgga 
      tagtgatag agattagta attaggcag attttgtat 
      tagttctgt gataagaat tttatacat aagttagtg 
      gttcaaatt ttattcatt actgcctta acgcgaaat 
      tatagttca aatgtattt tcttgcagc agttgagtt 
      tccttagcc gtgatattt ttgcagggt ttgatggaa 
      gggcccaag gttctccct taacttaag ttagttacg 
      cagctatga cttaagtac gtatcgttt tggcgtgca 
      taccttcct gtttgttca tgcatgcac gattccagt 
      caaccaaac atccctatt ttgttcaaa actgttaag 
      tgataaagg gttaagtgt ttcatgtgc aattctcgt 
      acctggcgg tacaaagaa attatttat tgtaataac 
      acggagtta gatttagca accgtacaa acctcagtt 
      ctttgttgt attgttttg ggcatgctg gagcatgtt 
      tttggttat acgtttttt taattagct ttaaagaga 
      gtaaggccg acagaaaca atgtacata ccggtcgaa 
      taatttgtt ttgagacga cgtttctgg agtcttgcc 
      gactactca atgtgttta aatagggaa tagtaactt 
      aaccagttg taaaataaa tatattttt tatatgaac 
      gttgttagt aacttggga tacgataat tagaaatag 
      caaattttg gtacaaagt gtagaaagt caaagttta 
      ttattgcaa tgttcgagc aaattctgt tgtttaaag 
      catgcacta tgcatttca ttactatag tttatattt 
      atgtgtatg tgataaatg catgtgata aataaaata 
      tcttaaaga gtattttaa caaataacc atattgtag 
      agaaacttg taattaatt atttataat aaattgaac 
      gtttattga accgtgtca tacacagct aagtaccaa 
      cttttgact acctatcat cgaccagtt ttgtgccta 
      aactcgcac tcacagggt ctcaatgta ttgttactt 
      accgttgga gtgttgtta acatccctc tgtttgtca 
      cttgtttgt attattaac tcaataaat aagatcaga 
      caat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY